FastQCFastQC Report
Sat 31 Dec 2022
SRR5847918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137763
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT3820.2772878058695005TruSeq Adapter, Index 3 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC2920.21195821809919935No Hit
TGAATAGGGGGCTGAACACAGACAATTTAAAAAAACATGTGGTCAAAGGACC1620.1175932579865421No Hit
GCCAGAGGGCAAGCAGTGTGGCAGATCCTTCAACTGTTCGAGCTCCTTTCGA1590.11541560506086539No Hit
CAACTAGGGTCTTTCGCAGGGATCTGCTCATAGAGTAAAATAAAACATGACT1520.11033441490095308No Hit
ATTAAAGGGTGGAAAGGTAGTGATTCTCAAAAGTTATTCTCAGTCTTCCTTC1490.10815676197527638No Hit
ACTTGGGGGACTATTACAATTTGATAGCATTATATATCAGATTATCTCTCTT1480.10743087766671748No Hit
TAAAGGAGGGCACTGATGAGACCCATCTGGCGGATCTGGAGGACCAGGTGGC1420.10307557181536407No Hit
CCAGCCTGGGTGACAGAGACCCTGTGTCAAACAAAACAAAACAAAAACAAAA1410.10234968750680518No Hit
ACATCTGGGGCGGAGCTTCATGTCTTATGTAACCATAGTATAATTATCAAAA1380.10017203458112846No Hit
GAGGCCGAGGCAAGAGAATCGCTTGAACCCGGGAGGCGGAGGATGCAGAGAG1380.10017203458112846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)