FastQCFastQC Report
Sat 31 Dec 2022
SRR5847901.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847901.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111414
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT5970.5358393020625773TruSeq Adapter, Index 3 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC5640.5062200441596209No Hit
GCGTTTGGGAATGGGAGACTTTAACACTCCACTGTCAATATTAGACAGATCA1620.145403629705423No Hit
TAATGCGGGTCATCAGGTTGATGTGAGAAATGAGGGAGATGTCTTGAATGGT1600.1436085231658499No Hit
GGCGACGGGAGGAAATGTCCAATCAGGGAAGTAAGTACGTCAATAAGGAAAT1570.1409158633564902No Hit
GGAGGTGGCAACAAAAGTAACAGATCCCAAAACAAAGGCCAGAAGCGGAGTT1500.13463299046798427No Hit
CGTAATGGGTTAGCATGGGCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.13283788392841114No Hit
CATACAGGGAGAACTGCATCAGGATATGGTGGTACCAGGAGACGATAAAGTA1470.1319403306586246No Hit
TCATTAGGGCGTGGAGCTACTGCCCATGATGCTTTCCTAGTGCCTGGTTACT1470.1319403306586246No Hit
TCTGCAGGGATCACAGGCAAGGAAGATGCTGCCAATAACTATGCCCGAGGGC1430.12835011757947834No Hit
AGTCAAGGGAGGGTTCAAGGCCAAGCTGAAGTTGTTGTCCTCTATTTTCCAC1410.1265550110399052No Hit
GCACATGGGTGGCAGAGAGTATAATGAAGAATCTTAGGCGGGTGCACCCAGT1410.1265550110399052No Hit
GTTGATGGGCACTTTACAAAGAAGCAATCACTCAATGAAACTGCTGGCTTTT1400.12565745777011864No Hit
GCACCCAACCCCACATCTGGGTGGCCAACTTGGCAGGACTTGGCCAGCAGCT1350.12116969142118585No Hit
ACAATAGGGGAGTGAGAGACTTCGGCGGACATGGCTCCCAGCGTGCCAGCGG1340.12027213815139928No Hit
GTTGGAGGGCTTTCACCACCCACACCCGGCTGCGCTTCTCAGTGCCATATGT1340.12027213815139928No Hit
GAAACAGGGCGGGTGTTGGAATCCACACCAAACAATGGCTACCTCTATCACC1330.11937458488161272No Hit
TCTGCAGGTACCCTGAGAAAAGTGAGAAGAGAGATTGCAGCCCAGGTCCCTG1330.11937458488161272No Hit
CAATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGAC1320.11847703161182617No Hit
CACACCGGGCCGATCTCGCTGAGGGACAAGATCAAGAGGAAAGCTTCCAAAA1310.1175794783420396No Hit
ACGTAGGGGATTTCACATTTTAATAGAATTCTGAAATATAGATTTGACCCCA1310.1175794783420396No Hit
GCTGCAGGGAATATATGTTTCTAGTACTGGTGGTCATGATGAAAGAAACAGG1300.11668192507225303No Hit
GCTACCGGGAACATTCAGCAATATCTGATGACCAATAATGTACCGGAGGCAG1290.11578437180246647No Hit
AAGACAGGGGGAAAACAAAAATCAGAAGGCTGCATTCTAGAGGGCAGAGAAA1290.11578437180246647No Hit
CTGTCTGGGCATACAGTCTTATCACCTCACATCTTCCACTTGGGTATCTTGC1280.11488681853267992No Hit
GTGTGAGGGCATGAAGCATGGAGACCTGAATAAGTTCCTCAGGGCCCATGGG1280.11488681853267992No Hit
CGCGTAGGGGGACTCGCTTTCCGTGCGGTGCGGCGAGTGAGGCCCCGGTCTT1270.11398926526289335No Hit
GTCTTAGGGGCTCATGATTCCCAAATATACTTCTCAGGGAGCTCTGTCACCA1270.11398926526289335No Hit
GTGTAAGGGCTTCCGATCTTCACGAGGGGAGGCATTGAGGCAGCCAGCGCAG1260.11309171199310679No Hit
AACGCCGGGGCAAAAAATTTCCTGGGACATTTTTCTGGCTTATTTCATCCCC1260.11309171199310679No Hit
CCAGCCTGGGCAACAGAGCAAGACCCTGTCTCAAAAAATAATAAATAAATAA1260.11309171199310679No Hit
TGCATGGGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG1250.11219415872332024No Hit
TAATAAGGGGATCTTCAGATGGGGCTGCCATCTCGTTCAGGCCCCACGTCGC1250.11219415872332024No Hit
AATGCTGGGATTACAGGTGTGAGCCACTGCACCTGGCCTGTAGTAGCTTAAA1250.11219415872332024No Hit
CAACCAGGGCTGCAGGCTAATGGCAGGAAATGGTCATATTTGGGGTTTTTCC1250.11219415872332024No Hit
ATTGAGGGGCATGAAGAACTCATATAATTCAATAATAGAAAAATAATCTGAT1250.11219415872332024No Hit
TTTGCAGGGGACCTTCCTGTTTATAATTTTCTAATGGAATTCTCAAATTCAT1240.11129660545353368No Hit
ACCTTGGGAGTCAACATGATGGACGAGTTTGCCAGATATGCCAGGCTGGAAA1230.1103990521837471No Hit
CTGACGGGAATTTATATTTAGTAACTTCTGTGTTAATACTGGATAGCATGAA1230.1103990521837471No Hit
GACTGAGGGGAACGATGCCATCGGCAAAGTCTTTGTGGGCTACAACAGCACC1230.1103990521837471No Hit
AAATGTGGGGGTCTGCCAGAAGGATAGGTTGGAATATTTAAAACAAAAAATT1230.1103990521837471No Hit
TATTGAGGGACTTTCATGGGATATTAATATTGATCTTCATTTGTAGTAAACT1220.10950149891396053No Hit
CGACTTGGGATAGAGAATTTGCATACTTGCCAACATGCACAATTTTTGTGAT1220.10950149891396053No Hit
ACTCGCGGGAATCCGAATTATCAAGGTAGTCATTATTTGAAGCTGAAGTTTG1220.10950149891396053No Hit
ACGTCAGGGCGAATACATGTATGGATAAGGGATTTGTTCCAGGTCACATAGC1210.10860394564417399No Hit
GTTGACGGGATTGATGATGTGAAAACCTAAATTACTTGCAGCATAGTATTAC1210.10860394564417399No Hit
TTGTAGGGGCAAGGTAGGGGACTAGTGGATCTTATCATAGTTTTGGCTGACT1210.10860394564417399No Hit
GCACCCGGCTTGAATCATCCTTTTGTCCAGTATTATATCCATGCTGTATATG1210.10860394564417399No Hit
TATGTGGGGGCACCGTCATCACACACACTTCTATTTATATTTCTTTCTTTGA1210.10860394564417399No Hit
GCTTTGGGGGAGACGCTGATGGGAGGATGGACGTACTGGTGTCTGAGTGCTC1200.10770639237438742No Hit
TAGCGTGGGCAAGTGGCCCTGTTAAAGGCTCTTTATGGACATGGCCAGATCT1200.10770639237438742No Hit
GGTACTGGGAGAATTTAAAATATAGAATAAAGTATATACCAAGTACTTAAAC1200.10770639237438742No Hit
GCGTAAGGGGCTCTCCTTTGGGTTTCTTTCCTTTTACTTTTTCTTTTGAAAC1200.10770639237438742No Hit
TACAAAGGGGTGGCCCTGTTAAAGGCTCTTTATGGACATGGCCAGATCTCTC1190.10680883910460086No Hit
CAAAGTGGGAATACTGTTCTGTGTAGCTTAATCTATTTCTTTATACATCTCT1190.10680883910460086No Hit
TATATGGGATGGATGGTCAGATCCTACTGGGCTCTTCAAAGACATGGGAATA1190.10680883910460086No Hit
TGTACCGGGTTGACGAGTGTGGTGATGCTTTAGAAATTTCTGAAGGAGTCAC1180.10591128583481431No Hit
TTGTATGGGATTTTCTGTTCGTGCATGAATTCACTTGGGATAATGGCCTCCA1180.10591128583481431No Hit
GCTATGGGGGCCGGCCGATCCCACCTCTCCTCGACCCTGGACGTCTACCTTC1180.10591128583481431No Hit
GCACATGGGCTGTGAAGTTCAGTACCACATTATTTTTCATTTCGACCACAGC1170.10501373256502775No Hit
TTAGGCGGGTAGCCTAAGTATACTAAAAATAGAAGGTTATTTCCTTCACTCA1170.10501373256502775No Hit
TTCCCGGGATGGCTTTGAATGCAGCCCAACACAAATTTGTACACTTTCCTAA1170.10501373256502775No Hit
AACATTGGGGTTGCTACTGAAATGATGTACTTTGACGTAATAAAAAATCAAA1160.10411617929524117No Hit
CTAACTGGGACTCTTGAGCAACATGAACGTTTGTGTCATCTTGTAATACACA1160.10411617929524117No Hit
ACTTCTGGGAAGTAAAGGGCCGGACCTCTTGCTTCTTAAGTTATTTTTTAAA1160.10411617929524117No Hit
AGTTTAGGGAAGTGAAAGGAGAAACCCCTTATAAAACCATTAGCTCTTGTGA1160.10411617929524117No Hit
GTGCTTGGGGTAGAAATAGGTTTTATTATAACATAAATGAACTTACTATTGT1160.10411617929524117No Hit
AAAGAAGGGATGTCGTCACAAGGATATCTGGCAAAGGAAACCAAGCTGCTTC1160.10411617929524117No Hit
TACAATGGGGATAGGCTCCCACCATTTATGCTTCTTCACTGATAGGGTTGCA1160.10411617929524117No Hit
AATAGCGGGAATTCACACTGTATATAAAATGGAAACAAACTGATTGCTCAGA1160.10411617929524117No Hit
ATTCACGGGCTGCAGAGGTGGCAAGAAGATGGTGGTGTTATGATCATAGGCT1150.1032186260254546No Hit
ATCAATGGGAAGCAGCTCTTCTTAGGGGGTGTGGATCGGCATAAGCAGTTCT1150.1032186260254546No Hit
TTCAAGGGGGAATAATGAATATAAGAAAAAATGACGAAAAGTGCTAGGCCAG1150.1032186260254546No Hit
TTTAGTGGGATAATAATGGGAGACTTTAACACCCCACCGTCAATATTAGACA1150.1032186260254546No Hit
CCGTCAGGGGCCATTTTACTTTTTGCTTCTATGAGTTTGACTGCTCTCGCCA1150.1032186260254546No Hit
TCAAAGCGGGAAGGCTAATGTAATGACTGTAGATTATCTATGTCCACATTGT1150.1032186260254546No Hit
GCCCAGAGCCTTGAGTTTTGATTCCAGTACCTAGTCATTTTCTTCTTCACTA1140.10232107275566805No Hit
TACCTAGGGAGAAGTAACTGTGATTGGCCACTTTCATATTTTCAGCTCTGCT1140.10232107275566805No Hit
GCAATCTGGGATATGTGATGCGTCTTTATATATATATGTGATAGTTGTAAAT1140.10232107275566805No Hit
GCTTGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1140.10232107275566805No Hit
TACGCTGGGGCTGGTGTTCGGCCTGGGCGCGATCCAGGTGGTCGGAGGCTCG1140.10232107275566805No Hit
ACAGCCGGGTTATCAAAGAGATATATGTCATAAAAAAGCAAATGCTGGAATA1140.10232107275566805No Hit
GCGCACGGGGGAATAAAAGAAAGGAGACCGCAGAAGTAAAGAAGTGGGGAGT1140.10232107275566805No Hit
TGGTATGGGATATGCTGAAATTACAAAATGCTGATTACAAAAATGAAAAAAG1130.10142351948588149No Hit
CGAGGTGGGGTGGAAGAAGGGTGCAAAGAGTAAAGGAAGCAGAGAGGAGCAA1130.10142351948588149No Hit
ATCAACGGGACTTTGTCTGCATTAGTTCTTGTGCAAAAATAGTTGCTTACCA1130.10142351948588149No Hit
TTCTTCGGGTGTGTGGAAACATGATATTCAGTTGAAAAACAGTACTGTTTGT1130.10142351948588149No Hit
GCATTCGGGGGTCTCAGCAAGACATAGGTACTAATTTGGTCGAAGCAGATAA1120.10052596621609493No Hit
ATCGCGGGCTGTAAAGGAGTCTTGTTTATCCTCTAATGGCCAGGCTTTTGGG1120.10052596621609493No Hit
ATTCGCGGGTTGCAGAGGGGAGTCTCAACTCATGAGTTGTTATACTTTCATT1120.10052596621609493No Hit
GCTCAAGGGTACCTTCATGTTATATGTATAGGTATATATGACACATAAATGA1120.10052596621609493No Hit
TTCACGAGGAAGCTCTAGCTCCGGGCCTGAGTTTCCCCGAGGCTGCGTCGGA1120.10052596621609493No Hit
GTTCCAGGGTCCTAGATTTGTGACTATCCAGGGCAAGTGAAACCCTTACTAT1120.10052596621609493No Hit
GCTGATGGGAATGGGCACATTTATGTTTTTCATCTCTAGTTACTGTCATCTC1120.10052596621609493No Hit
AAGCCAGGGGAATGTAAAGCCAATTTGATGAAATGGTTCAGGTTGTAGCAGT1120.10052596621609493No Hit
GTATAGGGAGAACATTCCCAGTGCCCCTGGCTAGGGGCAGAGGGCACTGCCA1120.10052596621609493No Hit

[OK]Adapter Content

Adapter graph