FastQCFastQC Report
Sat 31 Dec 2022
SRR5847898.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847898.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187308
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC5940.3171247357293869No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT5010.2674738932667051TruSeq Adapter, Index 3 (95% over 22bp)
AAGACAGGGATACTAAGCATGACTTCAACATTAAGACTTGAATTATGAGTTA2720.14521536720268222No Hit
AATCTTGGGACAGCCAGGCGCCCCTGCCCGCCGCGGTGCCCGCCGCCTGAAG2720.14521536720268222No Hit
TAGAAGGGATTGTTTAAGAAAAATATACTGAATTTATAGAAAGCAAGTATTC2690.14361372712324086No Hit
TATCAAGGGGGTGGCCGCGAAGCTAGGGCCACCATGGCGGACCGCAGGCGGC2650.14147820701731906No Hit
ACTCAGGGGGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTAGGAGAAT2620.13987656693787773No Hit
AGCTTCGGGGAAAAAAGAAGCCTTAAGTAAGTGTTTCTATTAGAGAAAAGGA2600.1388088068849168No Hit
TCGCTCGGGACTTCCCGCTTCGTGTACTGCACGTAGTAGAGAGCGTCGCCGA2550.13613940675251457No Hit
GATAATGGGCAGGATGGGAGACTAGAATCTATGAACATGAATTGTCTCCTCC2540.13560552672603413No Hit
TAGCCGGGGTCAGCAATTCTGGGGCATTGATATGAAACACTAGATAAGTAGG2530.13507164669955368No Hit
TGCCTTGGGATTATACTTAAAGGTGCAATTTTGCTTGGTTCTATGGTGGGAG2500.13347000662011232No Hit
TGTCATGGGGTCAGAATGTGAAATTAAATAATGGTTTGAACAGAAAATTCAA2450.13080060648771008No Hit
AGCCATGGGGTGCATATGTGTGTAGAAATGTATTAGAATGAAATAACATTTT2430.1297328464347492No Hit
AGAGAGGGGGGAAGCGCCAGAGATGGCTGCTCAGCGAGGGATGCCCAGCTCC2410.12866508638178828No Hit
ATCAGCGGGCTTCATCAACCTGTTGGTTATTTTGAAATGTGTTGTTTACTTT2390.1275973263288274No Hit
GGGCTTGGGAACAGAAAAATAGTTTAATTTCTGTAGCTGTGTTCATTTTGCA2380.12706344630234692No Hit
AGCGGCGGGGAGCGACCAATGGGACCAAGAAGAAATCAAAAGGCAAATCCAA2380.12706344630234692No Hit
GATAACGGGAACTATCGTGGCTCCACGGGCTTTGGCCAGGACAGCATCCTCT2370.12652956627586648No Hit
TACATGGGGGGCGACGGAGCGGGGTGTGAAGATGGCGGACGAAGAGGCCGAG2370.12652956627586648No Hit
CTAGTCAGGGTCACTACTGACTTGTTGGCTCGCGGGATTGATGTGCAACAAG2360.12599568624938604No Hit
ATAGCAACGGGGCATTTTGTTGACCTCAGGGAACTTGCTCAATTTCAAGACA2360.12599568624938604No Hit
TACGCCGGGCATCACCATTGGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTG2350.1254618062229056No Hit
TCGGTACGGGGAGCCAGAGGCTGCTAGCCCCACCACCAGAACTCGCCCAGTG2340.12492792619642513No Hit
TCTGCCGGGGATCCCTGATAAATCCATCAGATCTCTTGAGACGTATTCACTA2330.12439404616994469No Hit
GACATTGGGTCAGGGCAAGTCTACAGTGCAAAGCAAAAGCAAGTTTATTAGG2330.12439404616994469No Hit
CGCTTAGGGGTATTAGTAACTCAAAATAAATTATCACTTCGAAAACTTGCTT2330.12439404616994469No Hit
ATGCAGGGGATTTCTGCCTGGAGCCAAGCGGGGAATTAGGAAACGAAGAGAA2310.12332628611698379No Hit
CACAGGGAAGTAAGTGTGTGTTAACAGACTGAATTCTACCCCTACCTCCCAT2290.1222585260640229No Hit
GAACAAGGGATTAAAACAAGATCTTAAACATTTGATAAAACTAGTAAAAATG2290.1222585260640229No Hit
TTTGCTGGGGCTCCAAATTCGTGTGTCCATTAGACATTTGGCTACATCTGGC2280.12172464603754243No Hit
TCAAGGGGGGTGCCCATGCGTGACCTGGACTGTGGACCCTCTGCTGCTCCGC2280.12172464603754243No Hit
GCCAAGGGGACCTTGGAAGTGGGAAGGAAAATATTTACTTTCCAGCAGTTTT2270.12119076601106199No Hit
GAGTTGGGGCGATCTGATCCTGGGGTCCTGTGACTGGCTTATTTCCCTTAGC2240.11958912593162065No Hit
GTCTTAGGGCATTATTTGTACCTGAGGAAAAAAAGCTGAAAACAACTCAAAG2240.11958912593162065No Hit
CGAGTTGGGACTTACTTTTTTCTGCACTTATAACTGATTTGGTTTTAAAATT2230.1190552459051402No Hit
CTCATGGGGACGGTAAGCTAGACCTTGAGGGCTAGTGTGTGTGTTTCTCTGT2210.1179874858521793No Hit
CACGCAGGGTGAAAAGCTGGTGTTTTTACAACTAGCATTGAATGCAGTCCAA2210.1179874858521793No Hit
GATCGAGGGTAGGCCGGGGAACTTAATAGTCATGTATTTCAAAATTTGGCTT2200.11745360582569886No Hit
ATTGGCGGGGAGATTGGTCAATGCATTTCATAATTCTTTTATGTCTCTTTTA2190.11691972579921839No Hit
CGTAGTGGGATGACCCTTTTCCTCCATAACTTTTGATTTCTAAGGAAAATAT2190.11691972579921839No Hit
GCTAACGGGAAAATCACCAAACTACAGATAATCGCAAATCTAGCAGAGGGAA2180.11638584577273794No Hit
GGTTCAGGGAAGTAACAAGCTTCTAGAGGGCCCGGTAATCACCTGGCCGGAT2180.11638584577273794No Hit
CATTCGGGGTCTTGTCCTGAAGTTTCTTGCATCCTGATTCTGGTTGTAACAC2180.11638584577273794No Hit
ATGAATGGGATCTCGAATGTCTGTTGGCTGCAATTTAAATAATATACGACCT2170.1158519657462575No Hit
GACCTTTCTTACTCCAAGTCAGAGTGGTGACTGTCATTGACAACATTTTAAG2170.1158519657462575No Hit
TCGCCTGGGTACATTCTTGTGTCTAGTCACTTGTGTGGGAATGAAGGAGGGA2150.1147842056932966No Hit
ACTATGGGACCAAAGATGTTTGGTAACTTGAATAGACTTAATAGACAGAAGT2150.1147842056932966No Hit
CTCTGGATTCACTGCTCAAAGACCTAGGTTCAAATTCTGGTTTTTCCCTGGG2140.11425032566681616No Hit
TTTCTGGGGCATTCTCTCAGTCTATTTCTCTCACATATATTTAGAACATGTA2130.11371644564033571No Hit
ACATGAGGGTGTTTGCTGAAATTTTGTCACCAAAAACCTTCTTAAAAGAGAA2130.11371644564033571No Hit
GACGCTGGGCAGGAGACCAAGGAATCCCAGAAAGTGGAGTTGAGTGAATCCA2130.11371644564033571No Hit
ATCTTTGGGGAGCCCCTCTAGCTACAGTCTGGCTTAAATGAAAGGGGAATTA2110.11264868558737481No Hit
GATACTGGGGGAAGATTGAATTGCTCAACTCTCTTTTTAGACATTGAACTAG2110.11264868558737481No Hit
TCTACTGGGTGGGGGTAGGAAAATAGCTGAAGATTCATGTGCATTATTTGAC2110.11264868558737481No Hit
CGTGGGGGAATATGAAAGAAAAAAATCCAATTTACCCAAAAGTTCTCAGAAA2100.11211480556089437No Hit
TTGCTAGGGCCAGTTCTCCTACACAGCTGTCTTCGGTGCCTACACTGCTTTC2100.11211480556089437No Hit
CTGCGCGGGGTTGTTTGTTGGTTTTCGTTTCTTGAGTTTTTGTTTTGTTTTC2100.11211480556089437No Hit
GGCTATGGGAGCCAATACTGGCCAATGCTTCACCAGTTAGTCATGCTCCAAG2100.11211480556089437No Hit
AATAAAGGGAGGCTGGAAAACCGATTTTGTTTTTGTTTTATTCTTGTGATTT2100.11211480556089437No Hit
ATTAGCGGGGTTCCAGCAGCTTGCCCCTTTATATGAAATTGTCATCTTTACG2100.11211480556089437No Hit
AGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCC2090.1115809255344139No Hit
TTAGCTGGGAACCTTACAAGTGTAATGAGTGTGGGAAAGCCTTTAGTGTGCA2090.1115809255344139No Hit
GTGCGCGCGGGAAAGAGAGCGGAGTTCATAAGCCAAAAGATTGTCAAACATC2090.1115809255344139No Hit
CGTAGGGGGTGTTGAGACGGAGTCTTGCCCTATTGCCCAGGCTGGAGTGCAG2080.11104704550793346No Hit
TACTATGGGAGTGACTCACAACCTCCCGACAGTGGCACATCCTTCCCAAGCG2080.11104704550793346No Hit
GACCTTGGGCAATCTCTCCAGCCTTCTTCGTTTTACTAAATGGCCACTTCAT2070.11051316548145301No Hit
GAATTAGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG2070.11051316548145301No Hit
TGATATGGGGATCTTACACTGGGGCAACAAGGACAGTGTTGGTAGGGTGAGA2070.11051316548145301No Hit
GCTGTAGGGCTTCAGGTCCACCTTCTTACTTTATTAAAAGGGAATCGTGGGG2070.11051316548145301No Hit
TTACACGGGGGACAATGCAGCCAGAAGCTGAGGGACCAGCACTTGGTGCCTT2070.11051316548145301No Hit
TACACATGGGATCTGGTAGTGGGGAACCTTAAATTTGGATTGAAATCAGGTG2060.10997928545497256No Hit
AGCCTTGGGACAAGCCACGGAGGATGCAAAGGAAGAGGGTCTGGAAGCCCAG2060.10997928545497256No Hit
GATTTGGGGACTTGTTTTCCAGTCTGCAGAACAACTTAGCTCTTAATTTGAC2060.10997928545497256No Hit
TCTTCAGGGCTACTGATTTAAACTTTGATCTTATAAAAAAGCCTACCTTCAC2060.10997928545497256No Hit
GACTTAGGGGAAATATGGGGACAAGATGTTGCGCATGCAGAAAGGTGACCCA2060.10997928545497256No Hit
CATTTAGGGGCTTGTCGGGTCTTCAGGGAGGTGGCCGCGCCGGGCGGAGAGG2060.10997928545497256No Hit
GTGGTCCGGGAAGAACTGCAAAGTCTGAGAAATAGGAAAAATGTCTGAGACA2050.10944540542849211No Hit
CTAGCTGGGAGTTGTTTTTGTGTGGAATCTTGTCTTTCCGTATAAGTTGTAA2050.10944540542849211No Hit
ACGATAGGGAGCATCTATTGATAAAATTGTATGATTTTTTTTCTCTTTAATT2050.10944540542849211No Hit
GCCAAAGGGTTAGGAACTCAGCTTTCTATGAAGTGCCTACTGATGGATAGAG2050.10944540542849211No Hit
TAAAGCGGGATAGTTGGGAATCAAATGATAATGCTGAAATAAATGCTCATGA2050.10944540542849211No Hit
TAGAACGGGATAGCCCCGGATTCAAAAATAATTAGGATTCAGATTCTGAAAA2040.10891152540201167No Hit
AGCTCAGGGATCACTCACAAATGATGTATTTCTACAGAACTTGGGAGATTCA2040.10891152540201167No Hit
CAATACTGGCCAATGCTTCACCAGTTAGTCATGCTCCAAGGATTGAGGTCAT2040.10891152540201167No Hit
TCTTTAGGGAGATGATAAATTTTAGGTTGTGTGTATGTTACTGCTTTTTCAC2030.1083776453755312No Hit
TCTGCTGGGTGGATCTTACTGTTTTGAGGTATGTTCCGTCAATTACCTAGTT2030.1083776453755312No Hit
CAGCGCGGGTATGGACGGGATGTTGGCCAGGATGGTCTCAATCTCCTGACCC2030.1083776453755312No Hit
TATAGCGGGAGAGTTAGTGGTTACCACAGGGGCCTGCAATGCCTAAGGCATT2020.10784376534905075No Hit
TGTCCAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2020.10784376534905075No Hit
CGAGCGGGGGCCCATCGATGAGCATTTTTAACATACTCCATAGTCTTTTCCT2020.10784376534905075No Hit
AAGTTTGGGTAGAAGCAATGTTAAATCATTCAGGTTTTATTTTCTGCCCTGA2020.10784376534905075No Hit
AACGGGGTCGGGGAGACGCGGAGCAGTACTCCAGAAATGCCTGCCGTGTCTG2020.10784376534905075No Hit
GTACTAGGGGGTTAAATTTTTCAGACCAACCAAATCTGACTCAGTGGATTAG2020.10784376534905075No Hit
TATTTAGGGGTAGGACAGAGACCACACTGGGTCGGCGGACACAAAGTCCATC2010.10730988532257031No Hit
GCGCACGGGAAGAAAAGAAAGGAAAAGTGAAGTAGGCATATGAAAGAAAAGG2000.10677600529608985No Hit
CTATAGGGGGTTTTCAAAACAAGCAAGAATTATTTGCCGCTTGGTTTAGCAA2000.10677600529608985No Hit
TAGCTCGGGAAATATCATAGAGAAACTTTATTGTCAAATGAATACCTAAATG1990.10624212526960941No Hit
GTCACAGGGCATGGCGCAGGATCAAGGTGAAAAGGAGAACCCCATGCGGGAA1990.10624212526960941No Hit
AATCGAGGGGGTCCATAAGCGTGAAAAGAGGCAGGAAGAGAAGTGTCAGGGA1990.10624212526960941No Hit
TCGCACGGGAGCTACGTGTGCCCCTCTCATGGTCATGGGTCTGCCCTTGAGC1990.10624212526960941No Hit
GATTGGGGAAGTCTTTATGGGTCTGTTTCTGTTGTTGTTTTTGCTGTTTCCT1990.10624212526960941No Hit
GCATAAGGGGAACAAATGAATGAATAAATTGTGTGTGTTTACATATATATCT1990.10624212526960941No Hit
GGGTCCGGGGGGTGCTTCTAGCACCGCCCTGAGGCAGTCATTCTGTTAGACC1980.10570824524312897No Hit
CGCTCCGGGAGGACTGAAGAGAGTTAGGGCCTTGCTGTGGGTTAGATTTTGG1980.10570824524312897No Hit
GATAATGGGAACAGGGTAAATTGATTTCTTTATAGTGTGTATAATTTCACTT1980.10570824524312897No Hit
TCGCGGGAGCCCTCCTTCCATGTTTCTGTCTCAGCCCCACCAGCACTCTGCC1980.10570824524312897No Hit
CTTACTGGGCTTTACAAATGTTGTTTCCATTCCATGCAGGACAATTAAACCA1980.10570824524312897No Hit
TTGAATGGGGGAAGGTTGATAGGCCTTACGACAGTCTGCCTACCTCACAGAC1970.10517436521664851No Hit
ATCCAAGGGCGATCTCAAAATGGGGCCCCCCGGAGCCGACAAATGGCCGCGG1970.10517436521664851No Hit
TCACTTGGGAGTAAAGGACTATTTGGTTAATTGACAATTCCTAACACAACAA1970.10517436521664851No Hit
GCTAGGGGCATTAATATGTAGGGTCTCATGTCCACATCAGATGTGCCTAGAG1970.10517436521664851No Hit
GCACACGGGGGCGAGGGCGCCGCACCCACACCGCGCTGCGCAGTTTTGTTCT1960.10464048519016807No Hit
AACAACGGGACATCGGTCCGTCCTGCTTCCAGCTGCTGCAGCGCGCCTTCGC1950.10410660516368762No Hit
ACGTAAGGGAGCATTAAATAAACGCTGTAATTTAAAATGGCACATCCCATTC1950.10410660516368762No Hit
TCGACAGGGTGAATAACAGAATTACCCAGTACCTTGATGTTTTCTCATTTCT1950.10410660516368762No Hit
AAGCCGGGAGCCAGCTGCAGTCCGTACCGTTCTTAGGTTAGCCAGAGACAGG1950.10410660516368762No Hit
CATCCAGGGACATTGATTATTACCAGTGACTTCCCATTATAGTTTTTGGATC1950.10410660516368762No Hit
AATTTTGGGAGGGAAAAAAATATAGTTTTCTATCTCTTTCAAGGGCAGAAGA1940.10357272513720718No Hit
AGCGCGGGGCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAA1930.10303884511072671No Hit
CCGTCCGGGTTATGGAAATGTAATAGAATCTCACACATTATTTTATAGAAAT1930.10303884511072671No Hit
CAATAGGGTAATATCTTTACTTCTATCATCCCAAATCAAAGAACTCTTCTCT1930.10303884511072671No Hit
ACGATGGGAGCAAAAGCAATACTAAGAGGGAAGTTCTAGCAATAAATGCTTA1930.10303884511072671No Hit
ACTAAGGGGGGAATGGTAGTTGAGAAGAAAACTATTTGCACACAACAGATTT1930.10303884511072671No Hit
ACACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1920.10250496508424627No Hit
ATTTGAGGGAATCCATGGACCTTGTGGAACTTGTGTCTCTTTTTCCCACGCT1920.10250496508424627No Hit
TGGCCCGGGAACTCTGATATAATACTGGTGCTTAGAACATTATTCAAAAATA1920.10250496508424627No Hit
GCACGAGGGGAAGAATAAAACTAGAACCCTGTCTCTCAGCATATAGAAGAAT1920.10250496508424627No Hit
TACGTTGGGCTTGACATATGCTATTATAGGATAAATTTATAAAAAATATACT1920.10250496508424627No Hit
TCATTAGGGGGATTTATTTCTGAGCTCTCTATTCTGTTCTATTGGTCTATGT1910.10197108505776582No Hit
TCCATAGGGGACTCACACAGACTTAAAGCCTGAAAACATCTTATTTGTGCAG1910.10197108505776582No Hit
AGGAAAGGGTTCAAAATTTTACATAAATATGTGATAAAATTTAGCTCATATT1910.10197108505776582No Hit
TCATTAGGGACCAAAGACAGAATGGGGAGCCTAGACTTCCACCCTTGACAGG1910.10197108505776582No Hit
GTCAGGGGTCTCTTAGTCAGCATCCTTCACCACACAGTTGGAATCGAGGTCG1910.10197108505776582No Hit
ATTCATGGGCAGGCTGAGGCAGCAGCTGAGGCCCTGTCCAAAGAGAACTTAG1910.10197108505776582No Hit
CAGCCTCCCATAGTGCTGGGACCCAATTAATTCTTTAGTCAACCAACTTCAT1910.10197108505776582No Hit
AATCTAGGGAACTGCTAGGATTATAGGCATGAGCCACCGCACCCAGCCTATT1900.10143720503128537No Hit
ACACTCGGGGATTGCCAGCCAGATAGGATCCTGCGAAAGATGGTCGGGGGCA1900.10143720503128537No Hit
CTATCTGGGATAGATTTAAAGGAACTGCATGAGTAAGCAATCACACATGTTA1900.10143720503128537No Hit
GCGCTAGGGCATGAAAATTGATTTCAAGTGCGACCACTAAATTGTCATGTAA1900.10143720503128537No Hit
CGCTGGGGGGAGAATTGCTTGAACTTGGGAGGCAGAGGTTGCAGTAAGCCAA1900.10143720503128537No Hit
ATTGCAGGGGGGCTGGTCCTGGTGCAATCCTTCTTTATAAGAAGTGTTATGA1900.10143720503128537No Hit
CTTTATGGGCTGTTCAGCCACTTGCAACACAATGTTTCCAACTCTCTAACAT1900.10143720503128537No Hit
TCATATCGGGATGAAAACAAAAGTTAACTCTTTGAAAAGATCAACAAAAATT1890.10090332500480492No Hit
TAAACTAGGGAGCCTAGGAGGCTGGGCCGGAGGGAGGCGGAGGAACCGGTGT1890.10090332500480492No Hit
GGTCAGGGGCTTGGTAACAAAGTCTGTACATATTATGTATAAAATGTGTATT1890.10090332500480492No Hit
CTTAAAGGGAGAAGAGCCCAAGCCGGTGGAGGCCCCGGCAGCTAATTCCGAC1890.10090332500480492No Hit
CTTAAAGGGGAGGAAGAAGAGTGTTTACAAAGTCCTGTAGCCCCCTCACCTT1890.10090332500480492No Hit
TATGTAGGGAGCATCACTATTTTAGATTGCAAAAATACCTTAATTGTGTGGA1890.10090332500480492No Hit
TCTAAAGGCATGCCTGAGGCGATCTTTTATGCTTTTAAAAATGTTTCCCGTT1890.10090332500480492No Hit
GTTTATGGGGGACTGGGTTCTAGTGGGATGCGTTTGCTTGAACCCAGACCTT1880.10036944497832448No Hit
GGAGTGGGGAGACGGGGAAGCGGAGCCAACATGCCAGTGGCCCGGAGCTGGG1880.10036944497832448No Hit
GCAAACGGGCAGTTGTTTTGGAAATTTTATGTAAAATTAAAACTATCAGTAT1880.10036944497832448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)