FastQCFastQC Report
Sat 31 Dec 2022
SRR5847897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186127
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC5670.30463070913945856No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT5650.30355617401021884TruSeq Adapter, Index 3 (95% over 22bp)
GATAACGGGAACTATCGTGGCTCCACGGGCTTTGGCCAGGACAGCATCCTCT3070.1649411423382959No Hit
AATCTTGGGACAGCCAGGCGCCCCTGCCCGCCGCGGTGCCCGCCGCCTGAAG2750.1477485802704605No Hit
TGCCTTGGGATTATACTTAAAGGTGCAATTTTGCTTGGTTCTATGGTGGGAG2610.1402268343657825No Hit
ACTCAGGGGGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTAGGAGAAT2560.13754049654268322No Hit
TCGCTCGGGACTTCCCGCTTCGTGTACTGCACGTAGTAGAGAGCGTCGCCGA2560.13754049654268322No Hit
GATAATGGGCAGGATGGGAGACTAGAATCTATGAACATGAATTGTCTCCTCC2550.13700322897806338No Hit
AAGACAGGGATACTAAGCATGACTTCAACATTAAGACTTGAATTATGAGTTA2540.1364659614134435No Hit
ATAGCAACGGGGCATTTTGTTGACCTCAGGGAACTTGCTCAATTTCAAGACA2540.1364659614134435No Hit
TCGGTACGGGGAGCCAGAGGCTGCTAGCCCCACCACCAGAACTCGCCCAGTG2510.13485415871958395No Hit
GGGCTTGGGAACAGAAAAATAGTTTAATTTCTGTAGCTGTGTTCATTTTGCA2480.13324235602572437No Hit
ATCAATGGGCGAGGACGCTCCCTGCTCCGCGTCTGCGCCGCCGCCGGAGTCC2480.13324235602572437No Hit
AGCCATGGGGTGCATATGTGTGTAGAAATGTATTAGAATGAAATAACATTTT2450.1316305533318648No Hit
CACGCAGGGTGAAAAGCTGGTGTTTTTACAACTAGCATTGAATGCAGTCCAA2440.13109328576724494No Hit
GCCAAGGGGACCTTGGAAGTGGGAAGGAAAATATTTACTTTCCAGCAGTTTT2440.13109328576724494No Hit
TATCAAGGGGGTGGCCGCGAAGCTAGGGCCACCATGGCGGACCGCAGGCGGC2420.13001875063800525No Hit
GACCTTTCTTACTCCAAGTCAGAGTGGTGACTGTCATTGACAACATTTTAAG2410.12948148307338536No Hit
CTGCGCGGGGTTGTTTGTTGGTTTTCGTTTCTTGAGTTTTTGTTTTGTTTTC2400.12894421550876553No Hit
TAGCCGGGGTCAGCAATTCTGGGGCATTGATATGAAACACTAGATAAGTAGG2390.12840694794414567No Hit
TAAAGCGGGATAGTTGGGAATCAAATGATAATGCTGAAATAAATGCTCATGA2380.1278696803795258No Hit
CACAGGGAAGTAAGTGTGTGTTAACAGACTGAATTCTACCCCTACCTCCCAT2360.1267951452502861No Hit
TTGCTAGGGCCAGTTCTCCTACACAGCTGTCTTCGGTGCCTACACTGCTTTC2360.1267951452502861No Hit
CTCATGGGGACGGTAAGCTAGACCTTGAGGGCTAGTGTGTGTGTTTCTCTGT2350.12625787768566624No Hit
GATAATGGGAACAGGGTAAATTGATTTCTTTATAGTGTGTATAATTTCACTT2340.1257206101210464No Hit
AGAGAGGGGGGAAGCGCCAGAGATGGCTGCTCAGCGAGGGATGCCCAGCTCC2330.12518334255642652No Hit
TTACACGGGGGACAATGCAGCCAGAAGCTGAGGGACCAGCACTTGGTGCCTT2310.12410880742718682No Hit
TCATATCGGGATGAAAACAAAAGTTAACTCTTTGAAAAGATCAACAAAAATT2270.1219597371687074No Hit
TACACATGGGATCTGGTAGTGGGGAACCTTAAATTTGGATTGAAATCAGGTG2270.1219597371687074No Hit
ATTGCAGGGGGGCTGGTCCTGGTGCAATCCTTCTTTATAAGAAGTGTTATGA2270.1219597371687074No Hit
TCTTTAGGGAGATGATAAATTTTAGGTTGTGTGTATGTTACTGCTTTTTCAC2260.12142246960408754No Hit
AGCGGCGGGGAGCGACCAATGGGACCAAGAAGAAATCAAAAGGCAAATCCAA2260.12142246960408754No Hit
GCTAGGGGCATTAATATGTAGGGTCTCATGTCCACATCAGATGTGCCTAGAG2260.12142246960408754No Hit
TCTACTGGGTGGGGGTAGGAAAATAGCTGAAGATTCATGTGCATTATTTGAC2250.12088520203946766No Hit
GAACAAGGGATTAAAACAAGATCTTAAACATTTGATAAAACTAGTAAAAATG2240.12034793447484782No Hit
AATAAAGGGAGGCTGGAAAACCGATTTTGTTTTTGTTTTATTCTTGTGATTT2240.12034793447484782No Hit
ATTGGCGGGGAGATTGGTCAATGCATTTCATAATTCTTTTATGTCTCTTTTA2220.11927339934560811No Hit
TTTGCTGGGGCTCCAAATTCGTGTGTCCATTAGACATTTGGCTACATCTGGC2220.11927339934560811No Hit
AGCTTCGGGGAAAAAAGAAGCCTTAAGTAAGTGTTTCTATTAGAGAAAAGGA2220.11927339934560811No Hit
ATCAGCGGGCTTCATCAACCTGTTGGTTATTTTGAAATGTGTTGTTTACTTT2220.11927339934560811No Hit
CTAGTCAGGGTCACTACTGACTTGTTGGCTCGCGGGATTGATGTGCAACAAG2220.11927339934560811No Hit
AGCGCGGGGCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAA2210.11873613178098824No Hit
GATTTGGGGACTTGTTTTCCAGTCTGCAGAACAACTTAGCTCTTAATTTGAC2210.11873613178098824No Hit
CTTGTAGGGAGTCAACATGATGTAGGGGCTCAGTATTACATGGCAGGGCTAT2200.1181988642163684No Hit
GAATTAGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG2200.1181988642163684No Hit
GTCTTAGGGCATTATTTGTACCTGAGGAAAAAAAGCTGAAAACAACTCAAAG2200.1181988642163684No Hit
GCTGTAGGGCTTCAGGTCCACCTTCTTACTTTATTAAAAGGGAATCGTGGGG2200.1181988642163684No Hit
GCGCTAGGGCATGAAAATTGATTTCAAGTGCGACCACTAAATTGTCATGTAA2190.11766159665174854No Hit
ATCTTTGGGGAGCCCCTCTAGCTACAGTCTGGCTTAAATGAAAGGGGAATTA2180.11712432908712869No Hit
TGTCATGGGGTCAGAATGTGAAATTAAATAATGGTTTGAACAGAAAATTCAA2180.11712432908712869No Hit
GAGTTGGGGCGATCTGATCCTGGGGTCCTGTGACTGGCTTATTTCCCTTAGC2170.11658706152250882No Hit
AGCTCAGGGATCACTCACAAATGATGTATTTCTACAGAACTTGGGAGATTCA2170.11658706152250882No Hit
TCTTCAGGGCTACTGATTTAAACTTTGATCTTATAAAAAAGCCTACCTTCAC2170.11658706152250882No Hit
TCAAGGGGGGTGCCCATGCGTGACCTGGACTGTGGACCCTCTGCTGCTCCGC2170.11658706152250882No Hit
ATGCAGGGGATTTCTGCCTGGAGCCAAGCGGGGAATTAGGAAACGAAGAGAA2160.11604979395788896No Hit
ATTCATGGGCAGGCTGAGGCAGCAGCTGAGGCCCTGTCCAAAGAGAACTTAG2160.11604979395788896No Hit
CGTGGGGGAATATGAAAGAAAAAAATCCAATTTACCCAAAAGTTCTCAGAAA2150.11551252639326912No Hit
TAGAAGGGATTGTTTAAGAAAAATATACTGAATTTATAGAAAGCAAGTATTC2130.11443799126402938No Hit
GACTTAGGGGAAATATGGGGACAAGATGTTGCGCATGCAGAAAGGTGACCCA2130.11443799126402938No Hit
GCGCACGGGAAGAAAAGAAAGGAAAAGTGAAGTAGGCATATGAAAGAAAAGG2120.11390072369940954No Hit
CTAGCTGGGAGTTGTTTTTGTGTGGAATCTTGTCTTTCCGTATAAGTTGTAA2120.11390072369940954No Hit
ACGATAGGGAGCATCTATTGATAAAATTGTATGATTTTTTTTCTCTTTAATT2120.11390072369940954No Hit
GACATTGGGTCAGGGCAAGTCTACAGTGCAAAGCAAAAGCAAGTTTATTAGG2110.1133634561347897No Hit
GTCAGGGGTCTCTTAGTCAGCATCCTTCACCACACAGTTGGAATCGAGGTCG2110.1133634561347897No Hit
GCATAAGGGGAACAAATGAATGAATAAATTGTGTGTGTTTACATATATATCT2110.1133634561347897No Hit
TCAGTCGGGAACCACTGATTCTGCTACACCACACTGTGCTGCTTCCATTAGT2100.11282618857016984No Hit
TCTAAGAGGGGGTTTATAAAAGAGTGGCTTTAAAATTTGAAATACTGGGAAA2100.11282618857016984No Hit
TCTGCCGGGGATCCCTGATAAATCCATCAGATCTCTTGAGACGTATTCACTA2090.11228892100554996No Hit
CCGTCCGGGTTATGGAAATGTAATAGAATCTCACACATTATTTTATAGAAAT2090.11228892100554996No Hit
GGGTCCGGGGGGTGCTTCTAGCACCGCCCTGAGGCAGTCATTCTGTTAGACC2090.11228892100554996No Hit
CGCTCCGGGAGGACTGAAGAGAGTTAGGGCCTTGCTGTGGGTTAGATTTTGG2090.11228892100554996No Hit
CAGCGCGGGTATGGACGGGATGTTGGCCAGGATGGTCTCAATCTCCTGACCC2090.11228892100554996No Hit
GGTCAGGGGCTTGGTAACAAAGTCTGTACATATTATGTATAAAATGTGTATT2080.11175165344093012No Hit
GTGGTCCGGGAAGAACTGCAAAGTCTGAGAAATAGGAAAAATGTCTGAGACA2070.11121438587631026No Hit
GGAGTGGGGAGACGGGGAAGCGGAGCCAACATGCCAGTGGCCCGGAGCTGGG2070.11121438587631026No Hit
CATTCGGGGTCTTGTCCTGAAGTTTCTTGCATCCTGATTCTGGTTGTAACAC2070.11121438587631026No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCC2060.11067711831169041No Hit
TGCAAAGGGGAATCTTAGGAAGAAGGTAAAAGAAAGGAGGCAAGAGAATAGT2060.11067711831169041No Hit
TAATGTGGGCTACCCAGCAGGGCCACCTGTACCAGCTACTGAATACACCGGA2050.11013985074707054No Hit
CCTGCATACGGGAGGCTTTATTTCTTCTTCAGTACTAGTACTACTGAAAACA2050.11013985074707054No Hit
CGAGTTGGGACTTACTTTTTTCTGCACTTATAACTGATTTGGTTTTAAAATT2050.11013985074707054No Hit
TCTGCTGGGTGGATCTTACTGTTTTGAGGTATGTTCCGTCAATTACCTAGTT2030.10906531561783084No Hit
GACGCTGGGCAGGAGACCAAGGAATCCCAGAAAGTGGAGTTGAGTGAATCCA2030.10906531561783084No Hit
TACGTTGGGCTTGACATATGCTATTATAGGATAAATTTATAAAAAATATACT2030.10906531561783084No Hit
GATACTGGGGGAAGATTGAATTGCTCAACTCTCTTTTTAGACATTGAACTAG2020.10852804805321098No Hit
TTGCTCGGGCTTCTTCCCAATTTTAACCGTGCTTCAGGCAGTTGGTGGAGGC2020.10852804805321098No Hit
GGTGAAGGGTGGTAAGCTGACTGTTGTCATTCTGGAGGCAAAGAACCTGAAG2020.10852804805321098No Hit
CGTAGTGGGATGACCCTTTTCCTCCATAACTTTTGATTTCTAAGGAAAATAT2020.10852804805321098No Hit
AAGAATGGGGATTGAGGTCTCCATCTTCTCTCCCTCACCCTCTCCGTCTCCT2010.10799078048859112No Hit
TGCATTGGGCATGGGTGTTTCACCTGTATCGGTATACACATTTATTTTCAAG2000.10745351292397126No Hit
CTATAGGGGGTTTTCAAAACAAGCAAGAATTATTTGCCGCTTGGTTTAGCAA2000.10745351292397126No Hit
ACTCACGGGTGGCTCATTTAATTCTCACAATAATCCTATGAGGCAGGTACTA2000.10745351292397126No Hit
ACAACGGGATCAGAATAAAGTTCTGTATTTTGGTTTTGTTGGCTGGAAAAGT1990.10691624535935142No Hit
CAATAGGGTAATATCTTTACTTCTATCATCCCAAATCAAAGAACTCTTCTCT1990.10691624535935142No Hit
GGCTATGGGAGCCAATACTGGCCAATGCTTCACCAGTTAGTCATGCTCCAAG1990.10691624535935142No Hit
TTAGCTGGGAACCTTACAAGTGTAATGAGTGTGGGAAAGCCTTTAGTGTGCA1990.10691624535935142No Hit
TACGCCGGGCATCACCATTGGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTG1990.10691624535935142No Hit
ATTATAGGGAAGACTTTTCCTAGAGTACCCTTTTTCATTTTTTTCTGATAAT1980.10637897779473156No Hit
CGTAGGGGGTGTTGAGACGGAGTCTTGCCCTATTGCCCAGGCTGGAGTGCAG1980.10637897779473156No Hit
GTTTATGGGGGACTGGGTTCTAGTGGGATGCGTTTGCTTGAACCCAGACCTT1980.10637897779473156No Hit
AACGGGGTCGGGGAGACGCGGAGCAGTACTCCAGAAATGCCTGCCGTGTCTG1980.10637897779473156No Hit
TCGACAGGGTGAATAACAGAATTACCCAGTACCTTGATGTTTTCTCATTTCT1980.10637897779473156No Hit
TTTCTGGGGCATTCTCTCAGTCTATTTCTCTCACATATATTTAGAACATGTA1970.10584171023011169No Hit
GTATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1970.10584171023011169No Hit
ACTATGGGACCAAAGATGTTTGGTAACTTGAATAGACTTAATAGACAGAAGT1970.10584171023011169No Hit
ACACACGGAAGAGTCAATGATTCTGTTCGACTAAATAGCTGTCCTTTCCAAC1970.10584171023011169No Hit
AGTCTGGGCATTCATCAACATGTGTCTCTGAATTTTATTCAGTAGTTGTCAT1970.10584171023011169No Hit
CTCTGGATTCACTGCTCAAAGACCTAGGTTCAAATTCTGGTTTTTCCCTGGG1960.10530444266549184No Hit
GCTAACGGGAAAATCACCAAACTACAGATAATCGCAAATCTAGCAGAGGGAA1960.10530444266549184No Hit
AGCCTTGGGACAAGCCACGGAGGATGCAAAGGAAGAGGGTCTGGAAGCCCAG1960.10530444266549184No Hit
TGCCATGGGCATTGAATAAAGGGCACAACAGAACTAGAAAACCTAAAAACTA1960.10530444266549184No Hit
CTTAAAGGGGAGGAAGAAGAGTGTTTACAAAGTCCTGTAGCCCCCTCACCTT1960.10530444266549184No Hit
GTGCGCGCGGGAAAGAGAGCGGAGTTCATAAGCCAAAAGATTGTCAAACATC1960.10530444266549184No Hit
TCGCCTGGGTACATTCTTGTGTCTAGTCACTTGTGTGGGAATGAAGGAGGGA1950.10476717510087198No Hit
AACTATGGGATCTGCTATGGGGGCAACAAGTCTGCTAACTCATTCCAGTGGT1950.10476717510087198No Hit
TATAGCGGGAGAGTTAGTGGTTACCACAGGGGCCTGCAATGCCTAAGGCATT1950.10476717510087198No Hit
ACGTAAGGGAGCATTAAATAAACGCTGTAATTTAAAATGGCACATCCCATTC1950.10476717510087198No Hit
CTTCCTGGGCATGGACTCCCTGAAATTGTATGCAAAATGTTGTTTGTACATG1950.10476717510087198No Hit
CAGCCTCCCATAGTGCTGGGACCCAATTAATTCTTTAGTCAACCAACTTCAT1950.10476717510087198No Hit
AAGCCGGGAGCCAGCTGCAGTCCGTACCGTTCTTAGGTTAGCCAGAGACAGG1950.10476717510087198No Hit
GAGTCGGGAAGGTGAACTAATCAATGTCACAGGATAAAAGTCAATATATAAA1940.10422990753625214No Hit
TATTTAGGGGTAGGACAGAGACCACACTGGGTCGGCGGACACAAAGTCCATC1940.10422990753625214No Hit
TGGCCGGGGCGATTGAGTATTCCACAGGCTGTGAGATAGATTGGAATGAAGG1940.10422990753625214No Hit
AGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCC1940.10422990753625214No Hit
TCGGCTGGGGAAGTAGGAACCCTCTTCCTATGACAGGAGATCATTCTGCTTA1940.10422990753625214No Hit
GATCGAGGGTAGGCCGGGGAACTTAATAGTCATGTATTTCAAAATTTGGCTT1940.10422990753625214No Hit
TAGCTCGGGAAATATCATAGAGAAACTTTATTGTCAAATGAATACCTAAATG1930.10369263997163226No Hit
AACAACGGGACATCGGTCCGTCCTGCTTCCAGCTGCTGCAGCGCGCCTTCGC1930.10369263997163226No Hit
TGTCCAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA1930.10369263997163226No Hit
TTGGAAAGGGTGAGGACACACTAAACATTTGATAACTGCAAATGTGCTTTAA1930.10369263997163226No Hit
TACGCAGGGTAACCGCTTTACCACTAATTTATTGTTTTGTGTTTTCATCTAT1920.1031553724070124No Hit
CTTAAAGGGAGAAGAGCCCAAGCCGGTGGAGGCCCCGGCAGCTAATTCCGAC1920.1031553724070124No Hit
GCACACGGGGGCGAGGGCGCCGCACCCACACCGCGCTGCGCAGTTTTGTTCT1920.1031553724070124No Hit
AATTTTGGGAGGGAAAAAAATATAGTTTTCTATCTCTTTCAAGGGCAGAAGA1920.1031553724070124No Hit
ACGCCAGGGGGGATAGCTATGTGTTTGCTGAAGCAGGTGGAGTGTCTCCAAA1920.1031553724070124No Hit
GCGAATGGGATGGTAGTGATAGTACTGACCTCTAATGTGTGCATTTGTGGGT1910.10261810484239256No Hit
GCGCAAGGGCAATAGAAAGATAAGACCTACTGAGGTCTTTTTCCCATCATTT1910.10261810484239256No Hit
ATCCAAGGGCGATCTCAAAATGGGGCCCCCCGGAGCCGACAAATGGCCGCGG1910.10261810484239256No Hit
TCGCGGGAGCCCTCCTTCCATGTTTCTGTCTCAGCCCCACCAGCACTCTGCC1910.10261810484239256No Hit
ATGAATGGGATCTCGAATGTCTGTTGGCTGCAATTTAAATAATATACGACCT1900.10208083727777272No Hit
GCGACGGGGCCACTGTTCTGAAAGGAGACATTGCTCTATGTCTGCCTTCGAC1900.10208083727777272No Hit
CTGTAGGGGTAGAAAGTCCGGAGGGCAACTTCCAATTTAATTCTGCTCTGTC1900.10208083727777272No Hit
ACGATGGGAGCAAAAGCAATACTAAGAGGGAAGTTCTAGCAATAAATGCTTA1900.10208083727777272No Hit
TTTGCCGGGGGGATGTGGGATCAAGGAGAACAGCCTTGGCAGCAGTGGCCCT1900.10208083727777272No Hit
TTCGGATGGGAGTGAAAATAATTTAAATCATTATTTGTATCTTAAGTCTACT1890.10154356971315284No Hit
AATCGAGGGGGTCCATAAGCGTGAAAAGAGGCAGGAAGAGAAGTGTCAGGGA1890.10154356971315284No Hit
ACACTCGGGGATTGCCAGCCAGATAGGATCCTGCGAAAGATGGTCGGGGGCA1890.10154356971315284No Hit
TACTATGGGAGTGACTCACAACCTCCCGACAGTGGCACATCCTTCCCAAGCG1890.10154356971315284No Hit
GATAATGGGGGGGTAAGAATGATAGGGGGATGTTCAAACATTTATTGTGATA1890.10154356971315284No Hit
TACTTAGGGAATGAGATTTAATCTTTTATACCAATGTGTAGCTCATGATAGC1880.10100630214853298No Hit
TATTTGGGGGCAAGTTTGGGATGAATGACAAGATTGTTATTGAAAAGCAGGG1880.10100630214853298No Hit
AAGTTTGGGTAGAAGCAATGTTAAATCATTCAGGTTTTATTTTCTGCCCTGA1880.10100630214853298No Hit
TCTACTGGGCTTAGAGGAAATTTGAACAAGTTGGAAAAAAACAATTTTTGTT1880.10100630214853298No Hit
TAGGTGGGGACTACATCATTCTCATCATTCCCAGACAAGTGGATTTTACTTA1880.10100630214853298No Hit
ATCGATGGGAATTTAGTAAACCAATCCTATGCATGGTTTCAGCACTAGCCAA1880.10100630214853298No Hit
ACAACAGGGTATCCCACTGGCTTGGTGTGTATACATTAGGGGAGGATAATCT1870.10046903458391314No Hit
TGATATGGGGATCTTACACTGGGGCAACAAGGACAGTGTTGGTAGGGTGAGA1870.10046903458391314No Hit
CGCTTAGGGGTATTAGTAACTCAAAATAAATTATCACTTCGAAAACTTGCTT1870.10046903458391314No Hit
AGTTTAGGGCATTGTGTCTTTTTTGAGGCGTTCCTTCACTTGTCCCAGATTT1870.10046903458391314No Hit
TTAGCTGGGGCTGCTGGTTGAGGCTGTGTGGGTGGGGGACGGGCCGAGGCGA1870.10046903458391314No Hit
TATGTAGGGAGCATCACTATTTTAGATTGCAAAAATACCTTAATTGTGTGGA1870.10046903458391314No Hit
CATAGAGGGGTTTAGCCGAGTACATTGCACTGTAAATCCCTGTTAAGTGGAT1870.10046903458391314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)