FastQCFastQC Report
Sat 31 Dec 2022
SRR5847896.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847896.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184604
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT5580.3022686398994605TruSeq Adapter, Index 3 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC5300.287101037897337No Hit
AAGACAGGGATACTAAGCATGACTTCAACATTAAGACTTGAATTATGAGTTA3150.17063552252388897No Hit
TGCCTTGGGATTATACTTAAAGGTGCAATTTTGCTTGGTTCTATGGTGGGAG2840.1538428203072523No Hit
TCGCTCGGGACTTCCCGCTTCGTGTACTGCACGTAGTAGAGAGCGTCGCCGA2780.15059261987822584No Hit
TAGAAGGGATTGTTTAAGAAAAATATACTGAATTTATAGAAAGCAAGTATTC2700.14625901930619054No Hit
GGGCTTGGGAACAGAAAAATAGTTTAATTTCTGTAGCTGTGTTCATTTTGCA2660.1440922190201729No Hit
GTCTTAGGGCATTATTTGTACCTGAGGAAAAAAAGCTGAAAACAACTCAAAG2630.14246711880565968No Hit
GCCAAGGGGACCTTGGAAGTGGGAAGGAAAATATTTACTTTCCAGCAGTTTT2630.14246711880565968No Hit
GAATTAGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG2570.13921691837663322No Hit
GGGTCCGGGGGGTGCTTCTAGCACCGCCCTGAGGCAGTCATTCTGTTAGACC2520.13650841801911118No Hit
AGCTTCGGGGAAAAAAGAAGCCTTAAGTAAGTGTTTCTATTAGAGAAAAGGA2480.13434161773309353No Hit
AATCTTGGGACAGCCAGGCGCCCCTGCCCGCCGCGGTGCCCGCCGCCTGAAG2470.13379991766158914No Hit
TCTTCAGGGCTACTGATTTAAACTTTGATCTTATAAAAAAGCCTACCTTCAC2470.13379991766158914No Hit
ATGCAGGGGATTTCTGCCTGGAGCCAAGCGGGGAATTAGGAAACGAAGAGAA2400.13000801716105825No Hit
ATAGCAACGGGGCATTTTGTTGACCTCAGGGAACTTGCTCAATTTCAAGACA2390.12946631708955386No Hit
GATAACGGGAACTATCGTGGCTCCACGGGCTTTGGCCAGGACAGCATCCTCT2380.12892461701804944No Hit
CACGCAGGGTGAAAAGCTGGTGTTTTTACAACTAGCATTGAATGCAGTCCAA2370.12838291694654502No Hit
AGCCATGGGGTGCATATGTGTGTAGAAATGTATTAGAATGAAATAACATTTT2370.12838291694654502No Hit
AGCGGCGGGGAGCGACCAATGGGACCAAGAAGAAATCAAAAGGCAAATCCAA2360.12784121687504063No Hit
TTACACGGGGGACAATGCAGCCAGAAGCTGAGGGACCAGCACTTGGTGCCTT2360.12784121687504063No Hit
GATAATGGGCAGGATGGGAGACTAGAATCTATGAACATGAATTGTCTCCTCC2320.12567441658902298No Hit
ATCAGCGGGCTTCATCAACCTGTTGGTTATTTTGAAATGTGTTGTTTACTTT2310.1251327165175186No Hit
GATTTGGGGACTTGTTTTCCAGTCTGCAGAACAACTTAGCTCTTAATTTGAC2310.1251327165175186No Hit
GGCTATGGGAGCCAATACTGGCCAATGCTTCACCAGTTAGTCATGCTCCAAG2300.12459101644601417No Hit
AGCTCAGGGATCACTCACAAATGATGTATTTCTACAGAACTTGGGAGATTCA2300.12459101644601417No Hit
GACCTTTCTTACTCCAAGTCAGAGTGGTGACTGTCATTGACAACATTTTAAG2300.12459101644601417No Hit
ATCTTTGGGGAGCCCCTCTAGCTACAGTCTGGCTTAAATGAAAGGGGAATTA2260.12242421615999653No Hit
GTGGTCCGGGAAGAACTGCAAAGTCTGAGAAATAGGAAAAATGTCTGAGACA2250.12188251608849213No Hit
GAACAAGGGATTAAAACAAGATCTTAAACATTTGATAAAACTAGTAAAAATG2250.12188251608849213No Hit
GCTGTAGGGCTTCAGGTCCACCTTCTTACTTTATTAAAAGGGAATCGTGGGG2240.12134081601698772No Hit
GAGTTGGGGCGATCTGATCCTGGGGTCCTGTGACTGGCTTATTTCCCTTAGC2230.1207991159454833No Hit
CTAGTCAGGGTCACTACTGACTTGTTGGCTCGCGGGATTGATGTGCAACAAG2220.1202574158739789No Hit
TACGTTGGGCTTGACATATGCTATTATAGGATAAATTTATAAAAAATATACT2220.1202574158739789No Hit
TAGCCGGGGTCAGCAATTCTGGGGCATTGATATGAAACACTAGATAAGTAGG2210.11971571580247449No Hit
GTGCGCGCGGGAAAGAGAGCGGAGTTCATAAGCCAAAAGATTGTCAAACATC2210.11971571580247449No Hit
ACTCAGGGGGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTAGGAGAAT2200.11917401573097008No Hit
CGTAGGGGGTGTTGAGACGGAGTCTTGCCCTATTGCCCAGGCTGGAGTGCAG2190.11863231565946566No Hit
AGAGAGGGGGGAAGCGCCAGAGATGGCTGCTCAGCGAGGGATGCCCAGCTCC2180.11809061558796126No Hit
TCGGTACGGGGAGCCAGAGGCTGCTAGCCCCACCACCAGAACTCGCCCAGTG2180.11809061558796126No Hit
TTTCTGGGGCATTCTCTCAGTCTATTTCTCTCACATATATTTAGAACATGTA2170.11754891551645685No Hit
CACAGGGAAGTAAGTGTGTGTTAACAGACTGAATTCTACCCCTACCTCCCAT2170.11754891551645685No Hit
CGAGTTGGGACTTACTTTTTTCTGCACTTATAACTGATTTGGTTTTAAAATT2160.11700721544495245No Hit
CTCTGGATTCACTGCTCAAAGACCTAGGTTCAAATTCTGGTTTTTCCCTGGG2140.11592381530194362No Hit
CGTGGGGGAATATGAAAGAAAAAAATCCAATTTACCCAAAAGTTCTCAGAAA2140.11592381530194362No Hit
ATCAATGGGCGAGGACGCTCCCTGCTCCGCGTCTGCGCCGCCGCCGGAGTCC2140.11592381530194362No Hit
TTGCTAGGGCCAGTTCTCCTACACAGCTGTCTTCGGTGCCTACACTGCTTTC2140.11592381530194362No Hit
ACGATGGGAGCAAAAGCAATACTAAGAGGGAAGTTCTAGCAATAAATGCTTA2140.11592381530194362No Hit
GACTTAGGGGAAATATGGGGACAAGATGTTGCGCATGCAGAAAGGTGACCCA2140.11592381530194362No Hit
GCTAACGGGAAAATCACCAAACTACAGATAATCGCAAATCTAGCAGAGGGAA2130.11538211523043922No Hit
CTAGCTGGGAGTTGTTTTTGTGTGGAATCTTGTCTTTCCGTATAAGTTGTAA2120.11484041515893481No Hit
TAGCTCGGGAAATATCATAGAGAAACTTTATTGTCAAATGAATACCTAAATG2110.11429871508743039No Hit
TGCATTGGGCATGGGTGTTTCACCTGTATCGGTATACACATTTATTTTCAAG2110.11429871508743039No Hit
CTGCGCGGGGTTGTTTGTTGGTTTTCGTTTCTTGAGTTTTTGTTTTGTTTTC2110.11429871508743039No Hit
TACACATGGGATCTGGTAGTGGGGAACCTTAAATTTGGATTGAAATCAGGTG2100.11375701501592599No Hit
TAAAGCGGGATAGTTGGGAATCAAATGATAATGCTGAAATAAATGCTCATGA2100.11375701501592599No Hit
CAATACTGGCCAATGCTTCACCAGTTAGTCATGCTCCAAGGATTGAGGTCAT2100.11375701501592599No Hit
ATTGGCGGGGAGATTGGTCAATGCATTTCATAATTCTTTTATGTCTCTTTTA2090.11321531494442158No Hit
TCAAGGGGGGTGCCCATGCGTGACCTGGACTGTGGACCCTCTGCTGCTCCGC2090.11321531494442158No Hit
TACGCAGGGTAACCGCTTTACCACTAATTTATTGTTTTGTGTTTTCATCTAT2080.11267361487291717No Hit
ATGTCAGGGACACATCTGTGATTTTTAATAGAAAGGATGAAAGTATTACATT2080.11267361487291717No Hit
TGTCATGGGGTCAGAATGTGAAATTAAATAATGGTTTGAACAGAAAATTCAA2080.11267361487291717No Hit
ATTAGCGGGGTTCCAGCAGCTTGCCCCTTTATATGAAATTGTCATCTTTACG2070.11213191480141275No Hit
TTTGCTGGGGCTCCAAATTCGTGTGTCCATTAGACATTTGGCTACATCTGGC2060.11159021472990835No Hit
TACTTAGGGAATGAGATTTAATCTTTTATACCAATGTGTAGCTCATGATAGC2060.11159021472990835No Hit
GACATTGGGTCAGGGCAAGTCTACAGTGCAAAGCAAAAGCAAGTTTATTAGG2060.11159021472990835No Hit
GACGCTGGGCAGGAGACCAAGGAATCCCAGAAAGTGGAGTTGAGTGAATCCA2060.11159021472990835No Hit
ATTCATGGGCAGGCTGAGGCAGCAGCTGAGGCCCTGTCCAAAGAGAACTTAG2060.11159021472990835No Hit
GCCAAAGGGTTAGGAACTCAGCTTTCTATGAAGTGCCTACTGATGGATAGAG2060.11159021472990835No Hit
CGTAGTGGGATGACCCTTTTCCTCCATAACTTTTGATTTCTAAGGAAAATAT2060.11159021472990835No Hit
TCGCACGGGAGCTACGTGTGCCCCTCTCATGGTCATGGGTCTGCCCTTGAGC2050.11104851465840394No Hit
CCTGCATACGGGAGGCTTTATTTCTTCTTCAGTACTAGTACTACTGAAAACA2050.11104851465840394No Hit
ACAACGGGATCAGAATAAAGTTCTGTATTTTGGTTTTGTTGGCTGGAAAAGT2030.10996511451539512No Hit
CAGGAATGGGCACCAAAAGGTACCTGCCCTTGGAAAAGGATAAAAGCAAGAA2030.10996511451539512No Hit
TACATGGGGGGCGACGGAGCGGGGTGTGAAGATGGCGGACGAAGAGGCCGAG2030.10996511451539512No Hit
GGTTCAGGGAAGTAACAAGCTTCTAGAGGGCCCGGTAATCACCTGGCCGGAT2030.10996511451539512No Hit
ATTGCAGGGGGGCTGGTCCTGGTGCAATCCTTCTTTATAAGAAGTGTTATGA2030.10996511451539512No Hit
CTATAGGGGGTTTTCAAAACAAGCAAGAATTATTTGCCGCTTGGTTTAGCAA2030.10996511451539512No Hit
CATTCGGGGTCTTGTCCTGAAGTTTCTTGCATCCTGATTCTGGTTGTAACAC2030.10996511451539512No Hit
TACTATGGGAGTGACTCACAACCTCCCGACAGTGGCACATCCTTCCCAAGCG2020.10942341444389071No Hit
AGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCC2010.1088817143723863No Hit
GATAATGGGAACAGGGTAAATTGATTTCTTTATAGTGTGTATAATTTCACTT2010.1088817143723863No Hit
AACAACGGGACATCGGTCCGTCCTGCTTCCAGCTGCTGCAGCGCGCCTTCGC2000.1083400143008819No Hit
TGTCCAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2000.1083400143008819No Hit
AATCGAGGGGGTCCATAAGCGTGAAAAGAGGCAGGAAGAGAAGTGTCAGGGA2000.1083400143008819No Hit
TGGCCCGGGAACTCTGATATAATACTGGTGCTTAGAACATTATTCAAAAATA2000.1083400143008819No Hit
AACGGGGTCGGGGAGACGCGGAGCAGTACTCCAGAAATGCCTGCCGTGTCTG2000.1083400143008819No Hit
AAGCCGGGAGCCAGCTGCAGTCCGTACCGTTCTTAGGTTAGCCAGAGACAGG2000.1083400143008819No Hit
TCGCCTGGGTACATTCTTGTGTCTAGTCACTTGTGTGGGAATGAAGGAGGGA1990.10779831422937748No Hit
TTGCTCGGGCTTCTTCCCAATTTTAACCGTGCTTCAGGCAGTTGGTGGAGGC1990.10779831422937748No Hit
CAGCCTCCCATAGTGCTGGGACCCAATTAATTCTTTAGTCAACCAACTTCAT1990.10779831422937748No Hit
CATCCAGGGACATTGATTATTACCAGTGACTTCCCATTATAGTTTTTGGATC1990.10779831422937748No Hit
GCGAATGGGATGGTAGTGATAGTACTGACCTCTAATGTGTGCATTTGTGGGT1980.10725661415787308No Hit
ATGAATGGGATCTCGAATGTCTGTTGGCTGCAATTTAAATAATATACGACCT1970.10671491408636867No Hit
GATACTGGGGGAAGATTGAATTGCTCAACTCTCTTTTTAGACATTGAACTAG1970.10671491408636867No Hit
CTGTAGGGGTAGAAAGTCCGGAGGGCAACTTCCAATTTAATTCTGCTCTGTC1970.10671491408636867No Hit
CTCATGGGGACGGTAAGCTAGACCTTGAGGGCTAGTGTGTGTGTTTCTCTGT1970.10671491408636867No Hit
GCGCTAGGGCATGAAAATTGATTTCAAGTGCGACCACTAAATTGTCATGTAA1970.10671491408636867No Hit
TATGTAGGGAGCATCACTATTTTAGATTGCAAAAATACCTTAATTGTGTGGA1970.10671491408636867No Hit
TATCAAGGGGGTGGCCGCGAAGCTAGGGCCACCATGGCGGACCGCAGGCGGC1970.10671491408636867No Hit
TAGAACGGGATAGCCCCGGATTCAAAAATAATTAGGATTCAGATTCTGAAAA1960.10617321401486426No Hit
CGCTTAGGGGTATTAGTAACTCAAAATAAATTATCACTTCGAAAACTTGCTT1960.10617321401486426No Hit
ACGATAGGGAGCATCTATTGATAAAATTGTATGATTTTTTTTCTCTTTAATT1960.10617321401486426No Hit
GCATAAGGGGAACAAATGAATGAATAAATTGTGTGTGTTTACATATATATCT1960.10617321401486426No Hit
CAAGGTGGGATATTTAAAATGGAACACTTCTATGAAAAAAAATCTAAAATAA1950.10563151394335984No Hit
ATTTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1950.10563151394335984No Hit
GCGCAAGGGCAATAGAAAGATAAGACCTACTGAGGTCTTTTTCCCATCATTT1940.10508981387185544No Hit
GATCGAGGGTAGGCCGGGGAACTTAATAGTCATGTATTTCAAAATTTGGCTT1940.10508981387185544No Hit
ATCCATGGGAAATCCTGTTGAAAAGCCATGTTTAAGATTTAATTTGCCAAAA1930.104548113800351No Hit
AGCGCGGGGCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAA1930.104548113800351No Hit
AATTTTGGGAGGGAAAAAAATATAGTTTTCTATCTCTTTCAAGGGCAGAAGA1930.104548113800351No Hit
GCTAGGGGCATTAATATGTAGGGTCTCATGTCCACATCAGATGTGCCTAGAG1930.104548113800351No Hit
ACATGAGGGTGTTTGCTGAAATTTTGTCACCAAAAACCTTCTTAAAAGAGAA1920.1040064137288466No Hit
GAGTCGGGAAGGTGAACTAATCAATGTCACAGGATAAAAGTCAATATATAAA1920.1040064137288466No Hit
TATAGCGGGAGAGTTAGTGGTTACCACAGGGGCCTGCAATGCCTAAGGCATT1920.1040064137288466No Hit
TCGGTTGGGTACTTATCTCTAGAAAGAAGAAGGCATGCTACAAATAGGAAGG1920.1040064137288466No Hit
GATTGGGGAAGTCTTTATGGGTCTGTTTCTGTTGTTGTTTTTGCTGTTTCCT1920.1040064137288466No Hit
GACGCTGGGGCACCGTGTAGGCACTGCAGTGACAGTGTGGAATGAAATGGTT1910.1034647136573422No Hit
TGATATGGGGATCTTACACTGGGGCAACAAGGACAGTGTTGGTAGGGTGAGA1910.1034647136573422No Hit
AAGAATGGGGATTGAGGTCTCCATCTTCTCTCCCTCACCCTCTCCGTCTCCT1910.1034647136573422No Hit
ACGCCAGGGGGGATAGCTATGTGTTTGCTGAAGCAGGTGGAGTGTCTCCAAA1910.1034647136573422No Hit
TCTGCCGGGGATCCCTGATAAATCCATCAGATCTCTTGAGACGTATTCACTA1900.10292301358583779No Hit
TATTTGGGGGCAAGTTTGGGATGAATGACAAGATTGTTATTGAAAAGCAGGG1900.10292301358583779No Hit
ACGTAAGGGAGCATTAAATAAACGCTGTAATTTAAAATGGCACATCCCATTC1900.10292301358583779No Hit
TTAGCTGGGGCTGCTGGTTGAGGCTGTGTGGGTGGGGGACGGGCCGAGGCGA1900.10292301358583779No Hit
TAAATGGGGATAACATTACCTATGCTAATTACAGCATGTTGTCCAACACCAA1900.10292301358583779No Hit
TCGCGGGAGCCCTCCTTCCATGTTTCTGTCTCAGCCCCACCAGCACTCTGCC1900.10292301358583779No Hit
CTTAAAGGGGAGGAAGAAGAGTGTTTACAAAGTCCTGTAGCCCCCTCACCTT1900.10292301358583779No Hit
CTTACTGGGCTTTACAAATGTTGTTTCCATTCCATGCAGGACAATTAAACCA1900.10292301358583779No Hit
TGGGCAGGGAGGGGTACAGAAGAAACCCAGTTCCAAAAAGGTCCCTTCAGTG1900.10292301358583779No Hit
CGCTGAGGGGGTTTTCTCTTAAAAGATGCATGTATCATACGTGCTTTAAGCA1890.10238131351433337No Hit
GTCACAGGGCATGGCGCAGGATCAAGGTGAAAAGGAGAACCCCATGCGGGAA1890.10238131351433337No Hit
GCGCACGGGAAGAAAAGAAAGGAAAAGTGAAGTAGGCATATGAAAGAAAAGG1890.10238131351433337No Hit
TCATATCGGGATGAAAACAAAAGTTAACTCTTTGAAAAGATCAACAAAAATT1880.10183961344282896No Hit
GTCAACGGGATCCTGTTATTGATACAGATCATAGTTATAAGACAGGCTCATG1880.10183961344282896No Hit
TTTCTTGGGATGCTAAGAAAATAGTGACTATTTTGAAGTATGCTACTTCCCT1880.10183961344282896No Hit
GGTCAGGGGCTTGGTAACAAAGTCTGTACATATTATGTATAAAATGTGTATT1880.10183961344282896No Hit
AAGTTTGGGTAGAAGCAATGTTAAATCATTCAGGTTTTATTTTCTGCCCTGA1880.10183961344282896No Hit
TCTACTGGGTGGGGGTAGGAAAATAGCTGAAGATTCATGTGCATTATTTGAC1880.10183961344282896No Hit
GCAACCGGGGAGATCTAATGACTTCAGCATTGTTGGAAGCTTACCAAGAGAT1880.10183961344282896No Hit
GTTTTGGGGCAATTCTTCCGTGCTAACCCCTCATCAGCCTCCCTTGACCCCC1880.10183961344282896No Hit
CAGCCTGGCTAGGAGTCCTGCTTGTAATTTAGCCCAGGATCTCACCTGCAGA1880.10183961344282896No Hit
AATAAAGGGAGGCTGGAAAACCGATTTTGTTTTTGTTTTATTCTTGTGATTT1880.10183961344282896No Hit
ATTTGAGGGAATCCATGGACCTTGTGGAACTTGTGTCTCTTTTTCCCACGCT1870.10129791337132456No Hit
ACACTCGGGGATTGCCAGCCAGATAGGATCCTGCGAAAGATGGTCGGGGGCA1870.10129791337132456No Hit
GATAATGGGGGGGTAAGAATGATAGGGGGATGTTCAAACATTTATTGTGATA1870.10129791337132456No Hit
TCTGCTGGGTGGATCTTACTGTTTTGAGGTATGTTCCGTCAATTACCTAGTT1870.10129791337132456No Hit
GTCAGGGGTCTCTTAGTCAGCATCCTTCACCACACAGTTGGAATCGAGGTCG1870.10129791337132456No Hit
TCTAAGAGGGGGTTTATAAAAGAGTGGCTTTAAAATTTGAAATACTGGGAAA1870.10129791337132456No Hit
TTAGCTGGGAACCTTACAAGTGTAATGAGTGTGGGAAAGCCTTTAGTGTGCA1870.10129791337132456No Hit
ATTAAAGGGATTGATAATGTGGGACATATGTGTGATTCAATATATACATATA1870.10129791337132456No Hit
GTCAGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1870.10129791337132456No Hit
TGTGGGGGAGCTAGTCCTAGTGAACCTCACCCCGAGGCCCTGCATGGGCCAG1860.10075621329982015No Hit
AACTATGGGATCTGCTATGGGGGCAACAAGTCTGCTAACTCATTCCAGTGGT1860.10075621329982015No Hit
TAGTTAGGGCAAACACTCATTTCCCCGTCAGGAAGTTTGAAAAAAAGGCAAC1860.10075621329982015No Hit
AGTTACGGGGTAGCGTTGAGTGATACTTGGGTGATTTAGAGACCAGATGAAA1850.10021451322831573No Hit
CTTAAAGGGAGAAGAGCCCAAGCCGGTGGAGGCCCCGGCAGCTAATTCCGAC1850.10021451322831573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)