FastQCFastQC Report
Sat 31 Dec 2022
SRR5847895.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847895.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162472
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC5250.3231326013097642No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT4380.2695849130927175TruSeq Adapter, Index 3 (95% over 22bp)
TCTCTAGGGAGATGTAGATGGAACCAAAGTCCAGAAGTTACGCGTCACCCTT2370.14587128859126494No Hit
GTCTGCGGGAGCCTCAGGGAGCTTTTGATCAAATTGTGAAGAAAGACAATAA1910.11755871780983801No Hit
AGTTATGGGATTTCACCTGGCATTTGTTAGCACACAACTGACTGCCACCACC1890.11632773647151509No Hit
GTTACAGGGCTATTAAACTGATTAAATCTATTTTTTGAAAATGTCATCCACT1870.11509675513319219No Hit
GAGCTGGGGCTGGCGTGATTGATCATGACTTGGATCATGTGCTTCTCCCGCT1850.11386577379486927No Hit
CAGTCAGGGACTTTCTAATGAGGAACCAAGTGCTCAAAAGCAGTCATAAAGT1820.11201930178738491No Hit
TTAAAAGGGACAACAATGGTTGCTGATTTGGCATCAAAGAGGTGGCACTGTC1800.110788320449062No Hit
TAACGCGGGGTTCATGAAGGACTTCTTTTTGTTTCTAACTATAAACTTGGAT1760.10832635777241618No Hit
TAACTGGGATACAAAAACAGTAAAATCTTGTGCCCGATAAGATAAAAAGTAC1760.10832635777241618No Hit
TCAGCAGGGATACTTGAAGGAAATGTTTCACCTTATTTTTGGTCTTTGTTTA1720.10586439509577034No Hit
ACTCATGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG1700.10463341375744743No Hit
ATCTGAGGGGTGGTCTGTGTCCTGGGGGTTGGGGACCCCTGATTTAAAGCAC1700.10463341375744743No Hit
AATCAAGGGAGGATTTTCAAATTGGGTTTCCAGGTTAATTGTTACTTGCATT1690.10401792308828597No Hit
TCTATGGGGGAGCTGGGGCCGGGCTTCGGCGCAGAGGCAGGAAGAGGAAGCG1690.10401792308828597No Hit
TCTCTTGGGCTGAGCTGCCTTGGGAGAAGATGCTGATTGTCCTTGTCCAGAG1680.10340243241912452No Hit
GCAAATGGGATGGGATATTTCAAATTTAATTTTTTGAATTATTCAAATCCTG1660.10217145108080161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)