FastQCFastQC Report
Sat 31 Dec 2022
SRR5847894.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847894.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162491
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC5030.3095556061566487No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT4650.28616969555236904TruSeq Adapter, Index 3 (95% over 22bp)
TCTCTAGGGAGATGTAGATGGAACCAAAGTCCAGAAGTTACGCGTCACCCTT2250.13846920752533987No Hit
GTCTGCGGGAGCCTCAGGGAGCTTTTGATCAAATTGTGAAGAAAGACAATAA2190.13477669532466413No Hit
GAGCTGGGGCTGGCGTGATTGATCATGACTTGGATCATGTGCTTCTCCCGCT1940.11939122782184859No Hit
TCTCTTGGGCTGAGCTGCCTTGGGAGAAGATGCTGATTGTCCTTGTCCAGAG1800.11077536602027188No Hit
CGACCGGGATAATAGGGATTTGATAAGTGTTCATTACAGTTTAAAAAATGAG1790.11015994732015927No Hit
AGTTATGGGATTTCACCTGGCATTTGTTAGCACACAACTGACTGCCACCACC1780.10954452862004666No Hit
TAACTGGGATACAAAAACAGTAAAATCTTGTGCCCGATAAGATAAAAAGTAC1690.10400576031903307No Hit
AATCAAGGGAGGATTTTCAAATTGGGTTTCCAGGTTAATTGTTACTTGCATT1680.10339034161892043No Hit
ATCTGAGGGGTGGTCTGTGTCCTGGGGGTTGGGGACCCCTGATTTAAAGCAC1680.10339034161892043No Hit
TTGAACGGGTATCCCATTTAGGGTGGTTGGAGGGCCTTTTTAATTTAATTGA1670.10277492291880781No Hit
AAGCTTGGGGTTGCAGCCTGAGGCTGTCAGGTCCTCCCCCAGACACCTGCGG1640.10092866681846994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)