FastQCFastQC Report
Sat 31 Dec 2022
SRR5847893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160176
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC4990.31153231445410046No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT4880.3046648686444911TruSeq Adapter, Index 3 (95% over 22bp)
GTCTGCGGGAGCCTCAGGGAGCTTTTGATCAAATTGTGAAGAAAGACAATAA2070.12923284387174108No Hit
TCTCTAGGGAGATGTAGATGGAACCAAAGTCCAGAAGTTACGCGTCACCCTT1990.12423833782838876No Hit
GTTACAGGGCTATTAAACTGATTAAATCTATTTTTTGAAAATGTCATCCACT1830.11424932574168414No Hit
TAATAAGGCAACTTTATTAAGGATTACTTTGTCTGCCCACCACCTAGTGTAA1720.10738187993207472No Hit
CGACCGGGATAATAGGGATTTGATAAGTGTTCATTACAGTTTAAAAAATGAG1690.1055089401658176No Hit
TAACTGGGATACAAAAACAGTAAAATCTTGTGCCCGATAAGATAAAAAGTAC1680.10488462691039856No Hit
GAGCTGGGGCTGGCGTGATTGATCATGACTTGGATCATGTGCTTCTCCCGCT1630.10176306063330337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)