FastQCFastQC Report
Sat 31 Dec 2022
SRR5847801.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847801.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43688
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCCGTCT14333.2800769089910276No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGC12982.9710675700421167No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACT2890.6615088811572972No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCTTATAC2200.5035707745834096No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTA1510.3456326680095221No Hit
CACCATGGGCTGATAGATGATGCTGAAAACCCAACATGGAGTTTTTTAGTCT1280.2929866324848929No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCT1250.2861197582860282No Hit
GAAATTGGGGAGGAACAGAATCAATGAAACAAAACCAATGTTAGGGAATTGG1080.24720747115912836No Hit
GTATGTGGGGGTCAACTTAAGATTTGATTCAAGGCATTGATTTCCTAATTAA990.22660684856253432No Hit
TCATTTGGGCCATCAGTTGGGGTCATACCACTAGTCTCTGTCCTATACCCCA940.2151620582310932No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTATACAC930.21287310016480498No Hit
GAACGGGGGGTGCGCGCCCCGGATCCGGCGGCCACCCAGAGGAGAAGGCCAC900.20600622596594031No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAT870.19913935176707562No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAACTCGTATGC860.19685039370078738No Hit
TTCGTGGGGATTTGGAGCAGGGTGGTCACACTGACCTTGCAGAGTTCTGAGA860.19685039370078738No Hit
TAGAAAGGGGATTAAGACATTTTTGGTACTTGCATTTGACTTACGATGTATC770.17624977110419338No Hit
AATTGTGGGCTACTCACTCTCACTGCCCAAGAACTATCAAACTCCTGAGCCA760.17396081303790514No Hit
CTCGCAGGGGAGGGCTGGCTGGGCTCCAGCTGGCCTCCGCATCAATATTTCA750.1716718549716169No Hit
GCTATAGGGAGCAGCAGCAGCAGCTCGGGCGTGGGCAGCCCGGCCGTGACGC740.1693828969053287No Hit
CGTAATGGGGTATCCAAAAAAAATTATTGCCCAAAGCAGTACCATTGAGCTT710.162516022706464No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTA710.162516022706464No Hit
CGTGCCGGGTAGGGTAGGGGATCTTCTTGCGGGAGATTTCAGATGTGAAATC700.16022706464017578No Hit
GTACGTGGGAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATT690.15793810657388757No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGT680.15564914850759934No Hit
AAGTTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG670.1533601904413111No Hit
ATAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA670.1533601904413111No Hit
ACAGAGGGGGATCTATTGCAGGGTCTTTTTCAAGTTATTTTTACAGAAAGAA670.1533601904413111No Hit
CATCTAGGGATCTTGAGATGACTATATTTAGCATCAACAAAGGAATGCATTA670.1533601904413111No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC660.1510712323750229No Hit
CGCCATGGGGAGCGCTTCGGCGAGAGCCCCTTCCGCGACGAGGAGGAGTTGC660.1510712323750229No Hit
AGCCACGGGCATTCTCAAGGAAACCTTATCATAAGTGTAGTCTGTTGATGAG650.14878227430873467No Hit
GTACAGGGGAGTTTAAGAGCAGAACAGATCTTTTACTTTGGCCGCTTGAAAA650.14878227430873467No Hit
ACTCCTGGGAAACAAATAAACCAAAAAACAAATAGAGGGAAGGGGATATAGC650.14878227430873467No Hit
AACCAGGGGGGATAGCAAAGAATAATTTAGAGATATTAAATGAGAAGATGAC640.14649331624244644No Hit
TAGAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAA630.1442043581761582No Hit
AATTAGGGGTACTGCGTAAATAACAAAATTAAGACAGAAATAAATAAGTTCT630.1442043581761582No Hit
ATTTGCGGGACTCATTTTATTCATGCCTTGGTATGTAATTTGTCAAAATATC620.14191540010986997No Hit
CTCGTAGGGGAAAGGGATGCAAAAGATGCTGTTGATTTGATGAAGGCGCTGA620.14191540010986997No Hit
CAATAGGGGAGTGAAGAATGTCAATGGTAGTTTAATGGGAATAGCATTGACT610.13962644204358177No Hit
AATTTTGGGAAGGAGGCGATCTGCAGCTCACTTAAGGCCTACAACCACCACA610.13962644204358177No Hit
AATGCAGGGAATGATTTCAATGACACTGCCTTACAAAAAAATGAATTTCAGG600.13733748397729353No Hit
CTGTTCGGGGGAGGCTCTGTGACTGGGGCAACCTGCAAGGAGCTGGCCAGCC600.13733748397729353No Hit
TCTAGAGGGCACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACCACTA600.13733748397729353No Hit
ACATAGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.13733748397729353No Hit
GATTCCGGGGATTCCTTTTAATGTTCTTTGTTGTTTTCTTCTGGACCCACTG590.1350485259110053No Hit
CGGTCAGGGATTCATTGTGAATCCTGTAGCTTTTCTAGTTAACAAAAAATCG590.1350485259110053No Hit
TCCGACGGGGGCCCCCAGGGGAAAGGGCTGTGAGCTGCTCCGCCATTAAACT590.1350485259110053No Hit
CGGCGTGGGATGTCCAGAGTCCGTGAGGATTGGGAATCAGTCTAAAGCTGTG590.1350485259110053No Hit
AAACTGGGATAGCACCTCCTCCGAGTAGCCTATCAGGATCCCAGCAGTGGCT590.1350485259110053No Hit
GATCAGGGGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAA590.1350485259110053No Hit
TAACCAGAATATATAAGAAGCTCCAACAACTTTATAGGAAAAATATCAAATA580.1327595678447171No Hit
CTGTTTAAGGGGCAGGGCAGCATGAGGATGACTCCTTGGAGCTACAGGGGCT580.1327595678447171No Hit
GTTACTGGGATATCCAGAATATATAAAGAACTCTTAGAACCCAATAATAAAA580.1327595678447171No Hit
GGGCATGGGATCTCCACTTCTTCGCGTTCGGTGATGCTGCATTACCGCTCAG580.1327595678447171No Hit
TCGCTGGGTGTAAAAAACCTAATACTTTCCAGTCTTCTCAGCTTCCTGATCA580.1327595678447171No Hit
CTTAAAGGGACCTTTCCCTTCTCTCCCCAATACCCTTCCCAGCTTCTGGTAA570.13047060977842886No Hit
ACAGTTGGGACATCAACCTGGAGCTGCTGGAGTGTGTCTACCTGGTGTCTGC570.13047060977842886No Hit
CGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA570.13047060977842886No Hit
ACTACAGGGGAGACCCTGTCTCTAATAAAAAGAAAATGCATAGTATTTATTT560.12818165171214063No Hit
TAGAATGGGCCTCCACCTCTGCTCTGACCTCTCCCCATCTCTCTGCCCCTCC560.12818165171214063No Hit
GGTCTAGGGGGAGCTAACTATCCTAAATATATATGCACCCAATACAGGAGCA560.12818165171214063No Hit
ATTTTAGGGATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGGCTGG560.12818165171214063No Hit
ATTAAGGGGACAGTGACTTCTTAGTAAGCCCCTACATTATATGCATACTTGT560.12818165171214063No Hit
ATACGAGGGCATCAAAAAGTCTGAAAGAGCACAAATAAAAAAATCTAAGATT560.12818165171214063No Hit
GTGACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC560.12818165171214063No Hit
TACACCGGGACAACAAAAGCATTTGTTGACACTCGTGCTGTTAAGAGATTTG550.1258926936458524No Hit
TAGTGCGGGACCCCAGAGATTTAAGAAGAAAACCTCTAAATTTCAAAATTAT550.1258926936458524No Hit
AGGCCTGTAATTATGGTACTTTAAAAATTCAGAATATTTAGAAAAGCATCTA550.1258926936458524No Hit
TAAAGTGGGCATTGAGGATTGGTATAGCCATGATTTCAGTCATAGCAAGCTT550.1258926936458524No Hit
TATAAGGGGTAACAACATCTTTGAGAGAAGAACATCTCTTGGTGAAGTCTCT550.1258926936458524No Hit
GCTGTCGGGAAGGAGAAGCTGTGCTACGTCGCCCTGGACTTCGAGCAAGAGA540.12360373557956418No Hit
ATTGCTGGGGTATACTCTACTAACAACAGTAATCAACTACATTGACATCATT540.12360373557956418No Hit
CAAGCCGGCTTGAGCAATAGTTGTTTCTTTGTCAGTTTCAGTTACAATCATC540.12360373557956418No Hit
TTAGTGGGGTACCAGAGGAAAATTTCTTTATCACCTAAGTTCTTTCTAAATG540.12360373557956418No Hit
AATCGCGGGCAATCTTTGTGATATTACTTCGCTATTAAATAAAGAAAATTGG530.12131477751327596No Hit
GCACAAGGGGAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCG530.12131477751327596No Hit
TAACATGGGTATAATTATTTATGGTACAATTCTTGTACTTTAGCAAATCTGG530.12131477751327596No Hit
TTTGCGTGGGGATCACCAAAGGTCAGATTGTCATCAATGGATTATGAAAGGC530.12131477751327596No Hit
GCACCTGGGTCTTACAAAAATTTAATGGCACTATTTGTTCTGCCTTTCCATA530.12131477751327596No Hit
GTCAAAGGGCATACCCATGTTGATAGAGTTATTACCACACTCTAGGTCAATT520.11902581944698773No Hit
GTTAAGGGGGCTAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGT520.11902581944698773No Hit
CATGCTGGGGAAGTTGGAGTCCCATCCCCCAAGGCACATTTTTTTTTTTTTT520.11902581944698773No Hit
GTAATTGGGACCTAGCCCGGTGCGTCTGCATGCCCTGGCAGGGTTCCAGCAG520.11902581944698773No Hit
GGCGCTGGGATGTGACACCAGAGCTCACGGGAGATTCAGAAATCATTGTCAA520.11902581944698773No Hit
TTGTTAGGGCCGGCTGTGCACCCGGCCCTTCGGGCTGCTCAGCGTGCGCGCA520.11902581944698773No Hit
ATTGCTGGGGGGTCAAGTGACTAAGTGCATGTGGTGGATGCCTTGGCGATTA520.11902581944698773No Hit
TTGAAGGGGTGAAGAAAATTGAGCTCTCTGAAGTGACGGATACATTATTCAA520.11902581944698773No Hit
TAACTGGGCTTTAGGTAGTGAATTAGTTAAAATGAATCTAAATTGTGTGTTT520.11902581944698773No Hit
TCGTCCGGGGACCCTCCCTAATTTCCACTCCCCCCACCCCACTTCGCCTGCC510.1167368613806995No Hit
GGATTGGGGGTAGGTTGGGAAACATTGGAGAATGGTTTATCCAGTTTAATTA510.1167368613806995No Hit
GTCAGAGGGGGTTGATAAAGTATGTGTATCTTTACCTTTACTGGGCAGTTTT510.1167368613806995No Hit
AGTTTAGGGGATCTCCTGGCCTCAGATGATTCTTCCGCCTCAGCTTCCCAAA510.1167368613806995No Hit
GAGCTGGGGAGAATAAGGTCCGAGAGACCCCACGAGTGTGCGTAGGGAACGG510.1167368613806995No Hit
GTCAAGGGGGGTGAATACTTTTCAGGAATTCTTCCAGTTTTAAAATAGCCCC500.11444790331441128No Hit
AGTCATGGGGCAGCAAGAGCTGTAAGTATCAAGAATTTTATTTTACAATTCA500.11444790331441128No Hit
GGGGCGGGAGAATGGCGTGGGCCCGGGAGGCGGAGCTTGCAGTGAGCCGAGA500.11444790331441128No Hit
CGTTAAGGGAGTCAAATCCATTTTAGGTACTCTACTGACTTTTTCCTTCACT500.11444790331441128No Hit
GTTCAGGGGCACCTAAGGTGTCTGCATCCTCCTGCCAACGCATGGGCTCCAC500.11444790331441128No Hit
ACCTAGGGGATGTCACAGATCCGCAAAGGCCACGACACCATGTGTGTGGTGC490.11215894524812306No Hit
CAATAAGGGTGGAGTGGGTGAGAACGGAATTAAGGACAAAATGATAAGCTCC490.11215894524812306No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCC490.11215894524812306No Hit
ACTGCAGGGTGACACCAGATGTAGATATTTATCTGGAGCTGGAAAGAAAAAT490.11215894524812306No Hit
GAATTAGGGGGAAGGTGAAGATGGCGGTGGCCAGGGCCGGGGTCTTGGGAGT490.11215894524812306No Hit
TGAACAGGGCCAAGCTCACTTGGGCCAAGAGGTTTGAACATGAGCTTTTACC490.11215894524812306No Hit
AGATGGGGGCTTTCAGTTTATAGCAATATATTAGTCTACAAAAGAAGCCTTA490.11215894524812306No Hit
GCAGGCTGGGACACTAGTGGTGGTGGAGATGGAAAGGGACAGATTCAAGACA490.11215894524812306No Hit
ATAATAGGGCAGGGGAGTCTGAGGTCATGGCTTTATTTTTGCTAAGAGATGA480.10986998718183483No Hit
CTAAGGGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC480.10986998718183483No Hit
AGCACTGGGATTTATAGTTTATAGAGAATTTTCTTATTTGATCATTCACTTT480.10986998718183483No Hit
CTAAAGGGGGCATTTCGGGGCTGTCATCACCAGAAACGCCGGCTGCCGGCCG480.10986998718183483No Hit
TATTGAGGGGGACCACACACAAGTGTTATAGGAGGAGTCTGGCCCTTGAGTA480.10986998718183483No Hit
TTAGTGGGGGGGCCAGGACCCCCTCCTGCTCCTGCAGGCCCTGCAGACCCTG480.10986998718183483No Hit
CAATTAGGGGTGTGCTCATTGTGTCCCAGACCCATGCTAAGTCATCTATATG480.10986998718183483No Hit
CACATGGGGGACGGATGGACTTATTAATCAACTTCTCGAGAAATACTAAAGC480.10986998718183483No Hit
GAACAAGGGGGAGAGAAAGGCAGTCAGAGGAGAAAATGAGGTCCAGCTCGTC470.1075810291155466No Hit
ACTTATGGGTATCCTTTGAAAATAAGGATTTTAATTTCTACATGTTAATAAC470.1075810291155466No Hit
AGTATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC470.1075810291155466No Hit
TTAGGTGGGCGCACCCACCCTGTTCTCTACCTCTTCTATGAAGACATGAAGG470.1075810291155466No Hit
CTGCTGGGATAGTGACTAGACCATTTTACATTCCCACCAACAATGTACAAGG470.1075810291155466No Hit
GGTTGAATCTGAGATTTTGACTGGCTGGTTTATGAAGATTTCCTGCTTTAGG470.1075810291155466No Hit
TGGCTAGGGAGCTAAAACCCACTCTATGAGAAGGAAGATTACTGAAAAGCAT470.1075810291155466No Hit
TAATTGGGGTCTCATTAAACACCAGAGAGCACACGCAGGGGGGAAGCCTCAT470.1075810291155466No Hit
CTAACAGGGGAGTTGCTGCTGCAGCTGAGGTACAGCGGCGGTTTCTGAGGTT470.1075810291155466No Hit
GCATGCGGGTGAGTCTTACAGCCTGTGGGATGTTCTCCCCAAGCTTCTAACT460.10529207104925838No Hit
CATCCGGGGGTTAAGCCAGAACTTTATATTTTCAGAACAGTTTTGCTCTGTG460.10529207104925838No Hit
GCTACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC460.10529207104925838No Hit
GCTTATGGGGTTTTCTCATTTTTATTTGCTTTATGTCTGGATTGAAGATTAA460.10529207104925838No Hit
GATAATGGGCAGCTGATTATTTAATTACAGATTAGATTTCCTAGAAAAGGAG460.10529207104925838No Hit
CTGGATGGGGACAGCAGCTCCAAGCCCACCAGCTGTCCCAGCTGCAGGCCCT460.10529207104925838No Hit
CAGCGTGGGGAAATATGAAATACCTCATTTCAGACTTAAGAAGGTGGAGAAT460.10529207104925838No Hit
TACTGGGGGAACGAATTAAGAAGAAATAGAAAATTTAAACAAGCCAATAACA460.10529207104925838No Hit
AAGAATGGGGACTTGCCATGGTCGTCTTTGTCTGCCGGCCTGCTTCCTTGTA460.10529207104925838No Hit
TTAAATGGGAGAACTCCCCTTTGGTAATGCTTCTTTGTTTTTTTATGGCCCT450.10300311298297016No Hit
GTTGGAGGGGTACTGAAGGAAGAATACTTTTATCCTAGAATAGGATAGCCAG450.10300311298297016No Hit
ATACTGGGATGAGTTGAGTTATGTAAATTTTGTCATTGTAGTGTACATGGAG450.10300311298297016No Hit
ACACGAGGGATTGAGGAAAATTAATATCACAGCATAACCCCCACCCTTTACA450.10300311298297016No Hit
ATAGCGGGGAGGTATCAATGTAAGGACACAAGAAACATGAAAAGGAAACATA450.10300311298297016No Hit
AAATCCGGGGTAAGAATTAAAATGAATGATTTAAAAGTTTAATTCAAACAAT450.10300311298297016No Hit
TACTAGGGGATAATGAATGTACTTAACAACATGAACTATAAACTTAAAAATG450.10300311298297016No Hit
AATAAGGGATTCTAATCTTACCTGAAAGATATGTTAGTGTTTTATGTTTATA450.10300311298297016No Hit
CGAACGGGGCGATCTGACAGAGGGAAGCAACTGGATTTACAGATTTATTGTG450.10300311298297016No Hit
AAGTCGGGGGAGGCATTGATGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC450.10300311298297016No Hit
GTAATGGGGAAGTAATCCACCTGGCCTGCTCTTTTCATGTCTTAACATGGCA450.10300311298297016No Hit
ATGTCAGGGAGCTCACCCTTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGG450.10300311298297016No Hit
GTGTAAGGGGAGCAAGAGAGGCATCCTCACCCTGAAGTACCCCATCGAGCAC450.10300311298297016No Hit
AGACCAGGGGTGATAACAGTGTTAAATATGTGTGCATAAAGTCTTAAATGAA440.10071415491668192No Hit
TTATCAGGGAGCTAATTCTTGGGCAGCTTTCTTAGTTCTTTACAATGTTATC440.10071415491668192No Hit
AGCTATGGGACAGCCCGGGGCAGCCAGCAGTACCGGGCCCTGACCGTGCCCG440.10071415491668192No Hit
CCACCAGGGTGAGGGTTCCACCCCCATGACCCAAACACCTCCCACTAGATCC440.10071415491668192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)