FastQCFastQC Report
Sat 31 Dec 2022
SRR5847799.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847799.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71821
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCCGTCT17112.3823115801784995No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGC14231.9813146572729423No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCTTATAC2350.32720235028752037No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACT2190.3049247434594339No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCT1700.23669957254841897No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTA1130.15733559822336085No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTATACAC1110.15455089736985003No Hit
GTCTTTGGGCTGGCGGGCCGATACTACGCGCTCAAGAGCATGACGGAGGCGG1040.1448044443825622No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAACTCGTATGC1010.140627393102296No Hit
TTTCCGGGGACAACAAAACGAGTGGCACGATTTATTTCAAACTAGGCCAGCT920.12809623926149732No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTA890.12391918798123111No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC890.12391918798123111No Hit
TAATCTGGGGTCTGTTATCCTTGGTATCATCCATATGCTGTTTGGAGTCAGC820.11417273499394329No Hit
GTATATGGGACCTAATTTATTGAGAGTTTTTTAGCATGAAGCGTTGTTGAAT810.11278038456718788No Hit
CTTGCGGGGGTACTCTGGCATATTATAGGTAAACGGTTTAAAAGAACAATTT780.10860333328692165No Hit
ACTACAGGGAGACAAATCTGCTGGGTGCTGGGAGGGAATAAAAAGTAATGCA760.10581863243341084No Hit
ATAACACGGGGATTTGAATTCATCCAGAAAAACAAATGGAGGAGAAAAGCCT750.10442628200665544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)