FastQCFastQC Report
Sat 31 Dec 2022
SRR5847796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33172
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCCGTCT13614.1028578319064275No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGC13093.9460991197395394No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACT2860.8621729169178826No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCTTATAC2000.6029181237187989No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCT1400.4220426866031593No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTA1350.4069697335101893No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAACTCGTATGC1020.30748824309658745No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAT940.28337151814783557No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTATACAC820.2471964307247076No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC790.2381526588689256No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTA760.22910888701314364No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGT640.19293379959001566No Hit
CTGCAAGGGGTTCGGGTCTAGGCAAGTAGCTCACAGGAATATAGGAGAGGTA600.1808754371156397No Hit
TGTAAGGGATTCTCCAGGCAGCACATGCGGTGGCTCTGGGACATTGGTGGGA580.1748462558784517No Hit
TTATGCCGGGGAGGTGGCGGCGGCGAAGGATGCACCCGGCAGGCTTGGCGGC570.1718316652598577No Hit
AAGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC570.1718316652598577No Hit
CTCAGCGGGGCCATTAAGTTGTAAAATCAAACCAAGAAATCAAATTGTCAGT570.1718316652598577No Hit
TCTCCTGGGAAGTACTCAAAATTACATAGTTCATTTCTAAGAGAAGGCCATG560.16881707464126372No Hit
AGACACGGGGGGATTGCACCATTGCACTCCAGCCTGGGCAACAAGAGCGAAA540.16278789340407573No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTAT540.16278789340407573No Hit
GACCTTGGGTTCCGATCTCTGCGGGTGGGAGGGTGGAGCACTGGCTCCTTGA530.1597733027854817No Hit
AAAGTCGGGACTTCCATAGAAGAACATCACTTTTAACCTTGCTTTGGCGAAG520.15675871216688772No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCC520.15675871216688772No Hit
ATATGTGGGGACTGCAGCTGGAAGATGGCGGCGTCCGTGGTCTGTCGGGCCG520.15675871216688772No Hit
GGTTATGGGGAAAAAAGTAGACCTAGAAACCCTAAAAGAGGATTCTGAGTTC510.15374412154829373No Hit
TGCTTAGGGTCAAGTGGAGGAAAGGACTTGTTCTGATTCAGAAGATATTGGA480.14470034969251175No Hit
AAAGCAGGGATATTATCCACCTGCAAGCAGTGCCCAGTGGTCTATCCCAGCC480.14470034969251175No Hit
GTGACAGGGATTGAAACATAAAATTAATAATGAAAGTGAGAACAGATGTCTG480.14470034969251175No Hit
AAACTGGGGGGCAGATAAAGATTATTCTGTGACCGCCAATTCTAAGATTGTA470.14168575907391778No Hit
CGTGCAGGGGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA470.14168575907391778No Hit
TCATAAGGGCACCTTAGCAATGACAGGGTTTGAAAGGAATTCCCAAGTGACT470.14168575907391778No Hit
GCTTCAGGGAGGTTGAGAAAGGAATGGTTTGATATTTACCACAGCGCTGTGC470.14168575907391778No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTACCTCGTATGC460.1386711684553238No Hit
TCATTTGGGACAAAGAAGAGCATCCGTTCGAGAAGCGCCGCTCTGAGGGCGA460.1386711684553238No Hit
GTGTAAGGGGATATTATGACACTGACACTGCTATTTTGAAACCTGGAGGATG460.1386711684553238No Hit
AAGCTAGGGGAGATTGACTTGGACACTTCAGACCATGCCCACCTGGAGCACA450.13565657783672977No Hit
TGTTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC450.13565657783672977No Hit
ATACTAGGGCTGAGTTTTGGTTCTTATAAAAGTACTTTTTGTGTAGCTAGTG450.13565657783672977No Hit
ACGACGGGGATGGGGTTGTTTGTTTTTCACTGACCATCAGAGAAATGCAAAT450.13565657783672977No Hit
GAGTGGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA450.13565657783672977No Hit
CTTGAGGGGCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCT440.13264198721813578No Hit
ACTGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC440.13264198721813578No Hit
GCGCGGGGCTACGGGTGGGGGAATTTAATTAACCTGCATTGCCTCCCAACTG430.12962739659954178No Hit
GCACATGGGGATCTGCAAGCGGGTCGTCATCTTCTCCATGCAGACCCGCTAC430.12962739659954178No Hit
GGCCTGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC430.12962739659954178No Hit
ATACCAGGGCAGGCGGTAATGCTCACTCGCCGCCTCTCCCCCGCCCCGACTC420.1266128059809478No Hit
ACATTCGGGTGTCAACATGTACTTTGCTACTGGCTTCAGTGGTCACGGGCTC420.1266128059809478No Hit
TATAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA420.1266128059809478No Hit
GGCACGGGGGGGCACAGCGAGGCGCTAGGGGGAACGCTGGCCTCTGAAACTA420.1266128059809478No Hit
TGCAAAGGGAGGTGCAGACTGTGTTGGTGGCGGTAATTTTGTCCATCAAATG420.1266128059809478No Hit
ACTGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA420.1266128059809478No Hit
AGCATTGGGTGCCGTAACTTCCTACCTTGATTGTCTATTGCCACTCTCTTTG420.1266128059809478No Hit
AGTCTAGGGGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAG420.1266128059809478No Hit
TGTACAGGGCCCCGAGTGTGACTGGGAAGGAGAACTTATTCCTTACCAGGAA410.1235982153623538No Hit
CAAACCGGGCAGAGAAATTAAAGAAACCAAAGCTTTGCTTTTTTTTCTGGAT410.1235982153623538No Hit
CGTCATGGGATAGTGATTGTATTTTTTGTAATTTTCATTAAGCATTTAATTC410.1235982153623538No Hit
TCGTTGGGGAAACTAGTTATGACTGTTAACATGTTATTTCTCCCCATATTGA410.1235982153623538No Hit
AGTTGCGGGCTTTCTGGCTCTTTTCCACCTTTAACACTCTTATGTTGAAATC410.1235982153623538No Hit
ACACATGGGGAGGAAACATTCAGGAAATTCAGACTCGCCAGGACAACAAAGT410.1235982153623538No Hit
AGCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG410.1235982153623538No Hit
GTCAAGGGGACAGACCAAACCATTTGTATCTGGCATCACTTACTAACACACG410.1235982153623538No Hit
TGGCGAGGGACCCTGATGAATTTAGTTACCATTCTATGTCTCTTACTTCCTC400.1205836247437598No Hit
AAAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC400.1205836247437598No Hit
CTTCAGGGACCACAATAAGTAATAAAACATTTTGTAATTCATTCTGTAACAA400.1205836247437598No Hit
TTTGTGGCCAGACAAGAACACTAGCAAAAAGTGACATTTATTGAACACATGA400.1205836247437598No Hit
CAAATAGGGTGGTCCCTCTCCTCGGAAGAACTGCCCAGCCACGGGTTTTTAA400.1205836247437598No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTACCTCGTATGCCGTCT400.1205836247437598No Hit
TCGAGCGGGAAGTATATACTTTAAGAAATTCAGGTTTGACTGTATCTACTTC390.11756903412516581No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCTGTCTCTTATA390.11756903412516581No Hit
GTCAATGGGAGTAACATTTATTTGAATTCTGTCTTGGATTAGCTTTTCCAAA390.11756903412516581No Hit
TACTGAGGGGTACGTATTATTTTGTGTGGCATGCTAACTTTGAGCCGGGCAC390.11756903412516581No Hit
TACTACGGGATCAAAAGTGACTGCCTTAATAAGAATGGTTGCCTTAAAGAAA390.11756903412516581No Hit
CTCCGGGGTCTGTGAGCTGAGACTGGTTTTTACATTTTTTAATGGTGAAAAA390.11756903412516581No Hit
TGCTTCGGGAACTTGTGTGCACTACAGTTGTTCACCAGGGCCAGTGATTCAC390.11756903412516581No Hit
GTATATGGGGACATTGGATTTTAGCTATCTGGATACAAAGGTCAGTTTTCAC380.11455444350657182No Hit
AAAGCAGGGCAAGGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGC380.11455444350657182No Hit
GAACGCGGGATTCTGACAAACCTAAAAGTTGTATGGAACATATTTTTTAATG380.11455444350657182No Hit
TTCGGTGGGATTATTGCATTACCAAGAAACAGTAGCCAAAGATATTTGAAGA380.11455444350657182No Hit
TGGGCTAGGGCTGTATTTGCGGAGTGAATAATACTCTTTCCTCCTAGTGCAT380.11455444350657182No Hit
CAGCTGGGGGCGGCGGGCGGAACGGTGTCTCCTTCACTTCGCCCTCCAGCTG380.11455444350657182No Hit
TGTTAGGGGACTTAGAGCTGTATTCAAGTTGTTTGATACCAGACAGAAAGCT370.11153985288797781No Hit
TAGCATGGGATTGCTCAGAGCTTTTATTTCTTATACCTAGAATAAATATAAA370.11153985288797781No Hit
TACTACCGGGACAAGTAGCTCTCATTCTTTAACCTTAAATAAAAATGTTAGC370.11153985288797781No Hit
TTTAATGGGAGAGGTATGTCCTTTTATGTAATGGCCACCGCATTTAGTATTA370.11153985288797781No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC370.11153985288797781No Hit
TTAAGTGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA370.11153985288797781No Hit
TCGAATGGGAATGAATGAGTTTCTAGAAGTCTCACTTATCTTCCTAACCTTA370.11153985288797781No Hit
AAATATGGGAAGGTTTTCAGCAAGTTGTAACTTATTTTGGCCTAAAAATGAG360.10852526226938382No Hit
TAGTAGTGGGGTCAGCGGCGCTGAGGGGCATCTCCTGTTATCTGAAGGAGTT360.10852526226938382No Hit
TTTGTTGGGGTGACTTCGGGTCCCTGTCCCCACCCTTGTGCACTCACATGAA360.10852526226938382No Hit
GCTATGGGGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAATTTGATT360.10852526226938382No Hit
TCTTAAGGTTCCAAAGGGATTTAGCAGTGAGGCAGCAATGCTGAGTAGGTAG360.10852526226938382No Hit
GATGATGGGACAATATTCACATCTTTATCAACATCAGAAAACTCATAATGTA360.10852526226938382No Hit
TGTCGAGGGATTGGAAGTAGTTACAACTCAAGGATTTTGGTAGAAAGGTATG360.10852526226938382No Hit
CTTGTAGGGGAGGAGCTAAAATTGAATCATATAAATATTCAACTAAATCTAG360.10852526226938382No Hit
TTAAGGGAGTTAGTAAAGAAAAGTTTACCATCTGAAAAAGCTGGATTTTCTT360.10852526226938382No Hit
GTTCGCGGGGAAACACAATATGGTACTACATAGCAAGGGCTGTAAAAATGAC360.10852526226938382No Hit
GCATCAGGGAAGTTACTTCAATTATAAAATTGAATTTTGCAGACTAAATTAT360.10852526226938382No Hit
AGTCAAGGGAATCATACATTGCCTAAATCATAACTTGATGATGCTTGGGAAA360.10852526226938382No Hit
GAAGCGGGAAATGTTTTTAGGAAGTACTTACTGAAACATTTTTGTAAGACAT360.10852526226938382No Hit
TTCAGTGGGTCTAGTGGAGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGC360.10852526226938382No Hit
TTCAAAGGGATCCCTAAATGCATTTGTGTTAATAACTCTGAAACGTTTGTAT360.10852526226938382No Hit
GGTCGGGGGGAAATGTCGATTTTCAATAATAGACTTATATGCAGGCTGTCGT360.10852526226938382No Hit
TAGACGGGACGCTCTTCCGATCTTGTCTAGGGATTCCCCCCTGGTTAATTTT360.10852526226938382No Hit
GTTACAGGGCCGATCTATTGGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGG350.10551067165078983No Hit
GGCATGGGATATTGTACATGACTGCTAGTAGGTATGAAATGCATTTCAGAGT350.10551067165078983No Hit
GCTTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC350.10551067165078983No Hit
TGTCAGGGGCTTTTATATTTATTCATTATTTACCTGAAATTAAAATTTAACT350.10551067165078983No Hit
TATAACGGGCTTGGGACCTGGGAAGGTTTGCAGCACTTTGTCATCATTCTTC350.10551067165078983No Hit
TAATAAGGGCTGGGAATAAAAGGAACTTTTTTAAAAAAATTAATTAGTCCAC350.10551067165078983No Hit
GAGAATGGGATCGACGCCGGTCTGGGCCGCCCGCCGCGCCTGGCCGCCGCCA350.10551067165078983No Hit
ACCACGGGGGTTCACAGCTTGCTTGAAGAGAAAGGATGCTAGAATAAAGTAA350.10551067165078983No Hit
TTAGTGGGGATGTTGCTGCTGTGCTTGTACAGCCTGCAGAACTGCAAGCCAA350.10551067165078983No Hit
TTTGGCCGGGCAGTAAAAAGTATAGATAATTTAATTAACTTAGTGACACCAC350.10551067165078983No Hit
TCCAAAGGGATAAAAGTGTTAATTCTAATTGAAATGGTTCCTTTAGTAGATT350.10551067165078983No Hit
ATCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC350.10551067165078983No Hit
ACGAGGGGGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGG340.10249608103219583No Hit
GCCAATGGGATGACAATACAATATTCTAAAATAAGCATAGAAAAAGATATCA340.10249608103219583No Hit
TTCGCCGGGGTCTCAGAGTCTTTTTATTTTTCTTCTCAAAAATTAAGGTGAT340.10249608103219583No Hit
CTTACCTGGGTAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAG340.10249608103219583No Hit
TAAGTTGGGTTAGAGGTCCCTCTTTACCCCAGACCTGGGAGGGAAGTAGATG340.10249608103219583No Hit
GTTTGGGGGGTTCTATAGCACCACAGGGCGAATACAGTTAAATATAATTTAT340.10249608103219583No Hit
GTACAAACGGGATTATCAATAAGATAAAATAATGCATGTAGGTACCAACTGA340.10249608103219583No Hit
ATTCGCGGGAAGAGAGGCCCATATATTAAAATGTGTGAGGTAAAACCTGATG340.10249608103219583No Hit
TTAGGTAGGGGACGCAGCCTGCGGCCAGGCTTTTTATTTAATGTAAATAGTT340.10249608103219583No Hit
TCACTGGGGATCGGGTCAGGCAAGTCCATCCCGTCCTGAGAGCCCCAGGCCC340.10249608103219583No Hit
ACTCGCGGGATGTTTACCAAAAAACTCAAACAAATGAGCAGTGCCTGTTCAA340.10249608103219583No Hit
AGTGTAGGGTTTCCAGGAGGGAGGGGCGATTCTACCTTGTTTGCAGTGAATG340.10249608103219583No Hit
TCATAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA340.10249608103219583No Hit
CTGTTCGGGAGAGTTACAGGACATGATTAATGAAGTAGATGCTGATGGTAAT340.10249608103219583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)