FastQCFastQC Report
Sat 31 Dec 2022
SRR5847794.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847794.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33120
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCCGTCT13304.015700483091787No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGC12723.8405797101449277No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACT2810.8484299516908214No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCTTATAC1750.5283816425120773No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCT1300.392512077294686No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTA1190.3592995169082126No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAACTCGTATGC1100.3321256038647343No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTATACAC910.2747584541062802No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC760.22946859903381642No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGT650.196256038647343No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAT650.196256038647343No Hit
ATATGTGGGGACTGCAGCTGGAAGATGGCGGCGTCCGTGGTCTGTCGGGCCG650.196256038647343No Hit
CTCAGCGGGGCCATTAAGTTGTAAAATCAAACCAAGAAATCAAATTGTCAGT640.1932367149758454No Hit
CTGCAAGGGGTTCGGGTCTAGGCAAGTAGCTCACAGGAATATAGGAGAGGTA600.18115942028985507No Hit
GTTCGCGGGGAAACACAATATGGTACTACATAGCAAGGGCTGTAAAAATGAC590.17814009661835747No Hit
TTCAGTGGGTCTAGTGGAGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGC570.1721014492753623No Hit
TGATCAGGGATTACAGTTAGGTTCACATGAGAGAATGCTTGTAGAAGTACCT560.16908212560386474No Hit
GTCAATGGGAGTAACATTTATTTGAATTCTGTCTTGGATTAGCTTTTCCAAA540.16304347826086957No Hit
AAGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC540.16304347826086957No Hit
TGGGCTAGGGCTGTATTTGCGGAGTGAATAATACTCTTTCCTCCTAGTGCAT540.16304347826086957No Hit
GAGTGGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA540.16304347826086957No Hit
TGCTTAGGGTCAAGTGGAGGAAAGGACTTGTTCTGATTCAGAAGATATTGGA530.160024154589372No Hit
TGTAAGGGATTCTCCAGGCAGCACATGCGGTGGCTCTGGGACATTGGTGGGA530.160024154589372No Hit
ACATTGGGATATAAGACCTATAAGAACAGAAATTTTTTGTTTACTACATCTT530.160024154589372No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTA510.15398550724637683No Hit
TCGAGCGGGAAGTATATACTTTAAGAAATTCAGGTTTGACTGTATCTACTTC510.15398550724637683No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTAT510.15398550724637683No Hit
TCGTTGGGGAAACTAGTTATGACTGTTAACATGTTATTTCTCCCCATATTGA510.15398550724637683No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCC510.15398550724637683No Hit
TTCAAAGGGATCCCTAAATGCATTTGTGTTAATAACTCTGAAACGTTTGTAT510.15398550724637683No Hit
AGCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG490.14794685990338166No Hit
TCATAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA480.14492753623188406No Hit
AGACAGGGGAGCAAACCACCATGGCACATATATACCTATGTAACAAGCCTGC470.14190821256038647No Hit
CTTGTAGGGGAGGAGCTAAAATTGAATCATATAAATATTCAACTAAATCTAG470.14190821256038647No Hit
GTGTAAGGGGATATTATGACACTGACACTGCTATTTTGAAACCTGGAGGATG470.14190821256038647No Hit
GACCTTGGGTTCCGATCTCTGCGGGTGGGAGGGTGGAGCACTGGCTCCTTGA460.1388888888888889No Hit
CGTGCAGGGGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA460.1388888888888889No Hit
TAGTATGGGGACATCCCCTTAGCAATGAGATATTTTATTAATTAGAAAGAAA450.1358695652173913No Hit
AACCCCGGGGGCCTTTTGGCTTTCTCAAGGTGGGATTTAGAAATGGCTCCAT450.1358695652173913No Hit
CGTCATGGGATAGTGATTGTATTTTTTGTAATTTTCATTAAGCATTTAATTC450.1358695652173913No Hit
TCATAAGGGCACCTTAGCAATGACAGGGTTTGAAAGGAATTCCCAAGTGACT450.1358695652173913No Hit
GTCAAGGGGACAGACCAAACCATTTGTATCTGGCATCACTTACTAACACACG450.1358695652173913No Hit
GGTTATGGGGAAAAAAGTAGACCTAGAAACCCTAAAAGAGGATTCTGAGTTC440.13285024154589373No Hit
TGTGCAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC440.13285024154589373No Hit
TACTACCGGGACAAGTAGCTCTCATTCTTTAACCTTAAATAAAAATGTTAGC440.13285024154589373No Hit
AATCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA440.13285024154589373No Hit
GAACCAGGGAGTTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGTG440.13285024154589373No Hit
TGTACAGGGCCCCGAGTGTGACTGGGAAGGAGAACTTATTCCTTACCAGGAA430.12983091787439613No Hit
TGTTAGGGGACTTAGAGCTGTATTCAAGTTGTTTGATACCAGACAGAAAGCT430.12983091787439613No Hit
TATAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA430.12983091787439613No Hit
AGCATTGGGTGCCGTAACTTCCTACCTTGATTGTCTATTGCCACTCTCTTTG430.12983091787439613No Hit
ATACTAGGGCTGAGTTTTGGTTCTTATAAAAGTACTTTTTGTGTAGCTAGTG430.12983091787439613No Hit
AGTTGCGGGCTTTCTGGCTCTTTTCCACCTTTAACACTCTTATGTTGAAATC430.12983091787439613No Hit
CTTGAGGGGCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCT420.12681159420289853No Hit
TCTCCTGGGAAGTACTCAAAATTACATAGTTCATTTCTAAGAGAAGGCCATG420.12681159420289853No Hit
AAAGCAGGGCAAGGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGC420.12681159420289853No Hit
CAAATAGGGTGGTCCCTCTCCTCGGAAGAACTGCCCAGCCACGGGTTTTTAA420.12681159420289853No Hit
TGCTTCGGGAACTTGTGTGCACTACAGTTGTTCACCAGGGCCAGTGATTCAC420.12681159420289853No Hit
TCATTTGGGACAAAGAAGAGCATCCGTTCGAGAAGCGCCGCTCTGAGGGCGA420.12681159420289853No Hit
GATCGGGGGTCCATAACGGGGAGGACGTTGTGCTCCTCACCACCTGCAAGCA410.12379227053140097No Hit
CACAAGGGGATAAACATAAATGTCACAAAAACTTTAAAAGAAGTGCAATTCT410.12379227053140097No Hit
TTAAGGGAGTTAGTAAAGAAAAGTTTACCATCTGAAAAAGCTGGATTTTCTT410.12379227053140097No Hit
ACACATGGGGAGGAAACATTCAGGAAATTCAGACTCGCCAGGACAACAAAGT410.12379227053140097No Hit
AGTCTAGGGGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAG410.12379227053140097No Hit
AAATATGGGAAGGTTTTCAGCAAGTTGTAACTTATTTTGGCCTAAAAATGAG400.12077294685990338No Hit
TTCGCCGGGGTCTCAGAGTCTTTTTATTTTTCTTCTCAAAAATTAAGGTGAT400.12077294685990338No Hit
CTCCGGGGTCTGTGAGCTGAGACTGGTTTTTACATTTTTTAATGGTGAAAAA400.12077294685990338No Hit
ACTCGCGGGATGTTTACCAAAAAACTCAAACAAATGAGCAGTGCCTGTTCAA400.12077294685990338No Hit
AGCGCCAAGGGAGTAAACAAACATTAGAAATCCCCCTGTGGTTTATCAGACA400.12077294685990338No Hit
CAGCTAGGGAGAACAAGGTAGATGGGACTTATAACTTTCTGTAGTATGGTGT400.12077294685990338No Hit
ACATTCGGGTGTCAACATGTACTTTGCTACTGGCTTCAGTGGTCACGGGCTC390.1177536231884058No Hit
TTAGGCGGGGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTG390.1177536231884058No Hit
GCTTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC390.1177536231884058No Hit
TGTTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC390.1177536231884058No Hit
TAGCATGGGATTGCTCAGAGCTTTTATTTCTTATACCTAGAATAAATATAAA390.1177536231884058No Hit
GATGATGGGACAATATTCACATCTTTATCAACATCAGAAAACTCATAATGTA390.1177536231884058No Hit
AGCATGGGGATCTCTTGTGAGAAGGAATCTTTCACTTCTTTTGTTAAATTAA390.1177536231884058No Hit
GAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC390.1177536231884058No Hit
GCTTCAGGGAGGTTGAGAAAGGAATGGTTTGATATTTACCACAGCGCTGTGC390.1177536231884058No Hit
AAGCTAGGGGAGATTGACTTGGACACTTCAGACCATGCCCACCTGGAGCACA380.11473429951690821No Hit
TAGAAAGGGTGGAATAGTGCTGCTGCCAGCTTATTTTTCTGGTACTTGTATT380.11473429951690821No Hit
AAAGACGGGCTACTTCACTGTAATGTTGCTTTTCTTATTTTAGTCGGGCAAA380.11473429951690821No Hit
TTATGCCGGGGAGGTGGCGGCGGCGAAGGATGCACCCGGCAGGCTTGGCGGC380.11473429951690821No Hit
TATGCGGGGGGCTGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCAC380.11473429951690821No Hit
ACTGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC380.11473429951690821No Hit
AGTATAGGGGAGCTGAGCGATCCAGCTTCCACTTTTGTCCCTGAAACTGTCC380.11473429951690821No Hit
TTTGTAGGGTAACATTTTCATAGAAAATTCAGAAAACTGCTTGACACAGCAG380.11473429951690821No Hit
GTATAGGGGTGGTTTCACTTTGCATTTCCCTGATTATTAATGATGTTGAGTA380.11473429951690821No Hit
TGCACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC380.11473429951690821No Hit
CACGCAGGGAAAGATCATTTGTACAGTTCAGTGATCTAGTTTGGTGTGTCCT380.11473429951690821No Hit
TAAACAGGGCGCGGGGGGGTAGTGCCTGCATATCAGAGATACAAAAAGCATC380.11473429951690821No Hit
TGACCTGGGATTCATTGTTTAAAATAGTTAAAGATTCATGTTTTAAAAATAT370.11171497584541064No Hit
TGGCGAGGGACCCTGATGAATTTAGTTACCATTCTATGTCTCTTACTTCCTC370.11171497584541064No Hit
ACTCATGGGTGAGTACCAATCTGTGTATGCATGCGAATAAGCTACCCATGTC370.11171497584541064No Hit
TATGCTCGGGGCATCTGTTCTCTGAGGAACTCAAGTTTGGTTGCCAGAAAAA370.11171497584541064No Hit
TAATAAGGGCTGGGAATAAAAGGAACTTTTTTAAAAAAATTAATTAGTCCAC370.11171497584541064No Hit
TGTCGAGGGATTGGAAGTAGTTACAACTCAAGGATTTTGGTAGAAAGGTATG370.11171497584541064No Hit
GTTTCAGGGAGGGAGCGGGCCCGGGCGGCGCGCGATCGAGGTCGGGTCGCCG370.11171497584541064No Hit
GGCTATGGGCTGCTGCCCTTCCGCAAGCCGCTGATTATCTTCACACCTAAAT370.11171497584541064No Hit
TTAAGTGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA370.11171497584541064No Hit
CTGTTCGGGAGAGTTACAGGACATGATTAATGAAGTAGATGCTGATGGTAAT370.11171497584541064No Hit
TGCTCAGGGATTCAGAGCCAAGATAAATTCATATTATAGATTTTTATTTAAA360.10869565217391304No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCTGTCTCTTATA360.10869565217391304No Hit
GCCAATGGGATGACAATACAATATTCTAAAATAAGCATAGAAAAAGATATCA360.10869565217391304No Hit
TGCAAAGGGAGGTGCAGACTGTGTTGGTGGCGGTAATTTTGTCCATCAAATG360.10869565217391304No Hit
CAAACCGGGCAGAGAAATTAAAGAAACCAAAGCTTTGCTTTTTTTTCTGGAT360.10869565217391304No Hit
GAAAATGGGGGGGTTTAAAATCTTATTCTTATGACCCCAGGAAGCTGACCAG360.10869565217391304No Hit
TACAAAGGGATGTGTATGATGTGACTTCCATGTAGATATAAAAATGATTGTG360.10869565217391304No Hit
GGCGGTTGGGGTAATGCTGGTTCTCAGTGGAGTTTGTTCTGTGATGTTCAGC360.10869565217391304No Hit
TTTTTAGGGACGACACCTTCCTGGCTGTGCGCTACTCCCGGGGCCGTCTGAC360.10869565217391304No Hit
CGAGATGGGTCTGGGGCCTTGGGCACACCAGGGGCTGCAGGCTCCAAGGAGT360.10869565217391304No Hit
ATGTCTGGGGATCATTCAGAGAGACAGGTCGTTGCTCCGGAGTGATACAGAT360.10869565217391304No Hit
CAGCTGGGGGCGGCGGGCGGAACGGTGTCTCCTTCACTTCGCCCTCCAGCTG360.10869565217391304No Hit
GCGCATGGGTGCCATAGAAAGAAAAATAAAGTAGCCAATTCTAAAAGTAGCC350.10567632850241546No Hit
TGGTACAGGGCACTAGTCTAAGGGTGGAACAATTTCTTCTTGGTATAAGGTT350.10567632850241546No Hit
GGTAGTGGGAGGTACATTTTCCTCCCATTTTCTGAAAGTGTAGCAAATATGC350.10567632850241546No Hit
ACCACGGGGGTTCACAGCTTGCTTGAAGAGAAAGGATGCTAGAATAAAGTAA350.10567632850241546No Hit
ATAGTTGGGTAGCTAAGTCCCTCTCTGTACAGTAGAGTGGCTGCTGTGTGTT350.10567632850241546No Hit
GGCTACGGGAACTGAACTTTGACCTAAAACTAACCCTTATACAAAATTAACT350.10567632850241546No Hit
TCCAAAGGGATAAAAGTGTTAATTCTAATTGAAATGGTTCCTTTAGTAGATT350.10567632850241546No Hit
GATAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC350.10567632850241546No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC350.10567632850241546No Hit
GGTCGCGGGGGAACTAAAATATATAATGCCAAATGTGTTTTTGTCAATTACT340.10265700483091789No Hit
AATACTGGGGACCGCGCTCCGGTAACGGAAGAAACAAGATGGCGGCTGAAGG340.10265700483091789No Hit
TTGCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC340.10265700483091789No Hit
AGACACGGGGGGATTGCACCATTGCACTCCAGCCTGGGCAACAAGAGCGAAA340.10265700483091789No Hit
GCATCAGGGAAGTTACTTCAATTATAAAATTGAATTTTGCAGACTAAATTAT340.10265700483091789No Hit
TTCAAAGGGGCTGCCACGTAAAACTTCAGAGAAAAATCTTAAAAGCAGACCA340.10265700483091789No Hit
CACCAAGGGCTGAAATTGGGCACTGTGATACCGAAGTTAGTAAAGAGGCTTA340.10265700483091789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)