FastQCFastQC Report
Sat 31 Dec 2022
SRR5847758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88633
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC5350.6036126499159455No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT4420.49868559114551014No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3390.3824760529373935No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA3170.35765459817449485No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1640.1850326627779721No Hit
AGTGGCGGGGAAAGAAAGTATCTGTCAACAGTGACTTCTGTGTCCAGTGAAA1480.16698069567768212No Hit
ATACGCGGGTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.14215924091478344No Hit
CCAGCAGGGACTCTCAGATATCAAGATTTATTGTAAGGACAGAATAATTATG1230.13877449708347905No Hit
TTATGTGCCTGCTCCTCTTAGTAGCTCTTCTTTCTTGGTACTGCCTTGCCAG1200.1353897532521747No Hit
CTTCAAGGAGAACTACAAACCACTGTACAAGGAAATCAGAGAGGACAGAAAC1180.13313325736463844No Hit
GGTTCCGGGAATCTTTGAGATACATCCTGTCATCAGTATATTTCAGAATACA1180.13313325736463844No Hit
TGGGTAGGGCGCCCCTCAACAGGCATGACTCTGCAGAGATGACTCAGCACTG1160.1308767614771022No Hit
TCTGCTGGGACCCAGCCTTGGGTATTCCATTATAGCAACAGAAAATGGACTA1110.12523552175826158No Hit
GGTTGCGGGGTAATAGGAGCTGAATGATGACAATTTGGCTTTGTGTGCATTG1090.12297902587072534No Hit
TCAGGGGTCCCTAAAATACTGCATGTGTGTGCATACATGCATTTTCCTGGGG1090.12297902587072534No Hit
TAAGTAGGGCTGCCAGTTTTCTTCATTTCTGAATTGAGTTTTCTTTTCTTGA1080.12185077792695723No Hit
TGTAGAGGGAGCCCTTTCTTCTAAATCAGAATGTTGTCTTTTCCACAGATGG1080.12185077792695723No Hit
CTACTGGGGGAAGTGACTCTTTCCAAGATGATATTAACCCAGAAAGTTTAGG1070.1207225299831891No Hit
TCTACTGGGGGGATCCAAATACATAAAATAGATATGTTTTACCAGTAAATTT1070.1207225299831891No Hit
TCTTTCGGGTGACTACAGACACTTGCAAGCTTTCCTTTTACTTTCCTGTGAT1060.11959428203942098No Hit
AGCCATGGGCAATTCAAATATGTGTTGTTCAAGTGTCAGCTGTATTGAAAAT1060.11959428203942098No Hit
CTTAGAGGGCATAATGAATTCAAACAAATTCATGCCTATTAAAAGGAGGCTT1060.11959428203942098No Hit
AATTAAGGGCAGTTCTGTGTGGGGGCAGCCAAGGGGGTGCTGAGCCCGTTTG1050.11846603409565286No Hit
GCCCGGCTAGTTTTTGTATTTTTGGTACAGACGGGGTTTCGCCATATTGGTC1040.11733778615188473No Hit
TGTGGAGGTGAACAGTGACTTATTTTTATGTCAGGGTCATTAATTTTCATTG1030.11620953820811661No Hit
CAATAGGGGATCTTTTAGTTTCAATCACACATTAAAGTAAAAAATTTGTTCA1030.11620953820811661No Hit
TCCCGGGATGATTGTCACTGTCACTATCATCCTCTCATTGTCGTCATCACCA1020.11508129026434849No Hit
TTGTATGGGGCAGTCCAGTCTGGATATAATGAAAATGCCCTAGCAGCTTCCC1020.11508129026434849No Hit
CGCTACGGGGGCTAACTGAAATGATGGCGACTGGAACGCCAGAGTCTCAAGC1020.11508129026434849No Hit
AGACTGGGGTATACCCACCAACAGAAGTTTAAGAGTTTTGATGATGTCATGA1020.11508129026434849No Hit
ATCTAAGGGATAATTCAAATTACTTTTAGTTGTTTTATTTATAACTGTATTC1010.11395304232058037No Hit
TACATCGGGAACACTTATAAACCCATAAACCCATGTGGTTCAGTCTCTGAAT1010.11395304232058037No Hit
GCTAAAGGGGCTTTTCCGTGCTACCTGCAGAGGGGTCCATACGGCGTTGTTC1010.11395304232058037No Hit
GCGTTAGGGACCATTTTTAACTAGTGTTTTGACTGAAGATTATTATGAAAAG1000.11282479437681224No Hit
GTGCCTGGGCTGTGGGTCAGGAGATCAGAAATGGTTCTATTTCAAGGTCTCT1000.11282479437681224No Hit
TCAGTAGGGAGTGGGCCATTCATTTCTATAGGCAAAGAAAGCTCTAGACAGA1000.11282479437681224No Hit
TGCCACGGGCGGAAGCTCAGTGCTGTAGTCCAACTGTCCCTTGTGGGCACTG1000.11282479437681224No Hit
TCTTGGGCTCTTCTGTGACTGCTATTGAAAGGTATCCTTGGGAGGAGGAATA990.11169654643304412No Hit
TATTAAGGGGATCTTGCCAAGGGTGCCCAATTCACTCCAATAAGATAAACTT990.11169654643304412No Hit
CTCTCCGGGATATGATTGATATTTCAGTATTGATGTTAGCTTTGTTCGTGTA990.11169654643304412No Hit
CAGAAAGGGACTCACATACATATATGCATACACATGTGTGTGTGTGTGTGCA990.11169654643304412No Hit
CAAAGTGGGTAAAGTAGTAGTATGCACAATGAAGTTATAGCTTTGACCTAAT990.11169654643304412No Hit
GACTATGGGTTGTGATGAAAATGCAAAGGAGCCCGAAAATGCACTTGACAAG990.11169654643304412No Hit
GGTCCTGGGACAAACAAAGTGTTACATCTTCTGCATAAAATTACCTACTTAA990.11169654643304412No Hit
GTTGCCGGGCTCTCCACCTTAGTACTTTAGACTAATTGTGTTTGTCCGTCCA990.11169654643304412No Hit
AAAATGGGGTTCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGTTAC990.11169654643304412No Hit
CTTTTAGGGGATCTACGTATGGGATAATGATAAGAGATTATTTTAAATATGT980.110568298489276No Hit
ACGAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC980.110568298489276No Hit
ACATCAGGGAGCTGGGACCACTTGTGAAACGGCTAGTTGTGCCTAACAGACA980.110568298489276No Hit
TCGCGGGTTACAAACAAAAAAGTCACATCCTTGCCCTTTAGAGGCCAACAGT980.110568298489276No Hit
ATAATAGGGAGAGAAGCAGTCAGAGATGAGAGGATGGCAGCTAGCTGAAAGA980.110568298489276No Hit
TATATCGGGAGCTCAGCAAGGAAAGAATGGGCTCAGAAAAAGGAGGGCTGGC970.10944005054550787No Hit
GCACATGGGAGTCCTACCTGCCTGAAGACATAGCTGGTGGAGTTGAGATCTA970.10944005054550787No Hit
TGTAAAGGGCAAGAAGCTTTCTTCACCTATGAGAAACTGTAGACACACATAA970.10944005054550787No Hit
AGAAGAGGGTGCCAGAAATTGTTTCAAGATCTTTACATGGATTATTTCATTT970.10944005054550787No Hit
ATCATGGGGTAATCCATGTACTTTGTCCAATGCTGAAAGTAAGTATTTTTAA970.10944005054550787No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA970.10944005054550787No Hit
GCCCGCCTTGGCCTCCCAAGGTTCTGGGATTGAAGGTACGAGCCACCGCAAC970.10944005054550787No Hit
TTAACAGGGGGCTGGACTGAAATTACTAGGGCACTTGAGTATAAAATTTGAA970.10944005054550787No Hit
GTAACAGGGCATTCTCTCTCCATTTACTTCTGGTGGTTACCCTGACTCTTGA970.10944005054550787No Hit
AGACCAGGGGAATTTCGTTCTTAGTGCAGGTTACTTGTAGATTCTAGTCTTC960.10831180260173975No Hit
CATTTAGGGATCCGGTACATTTCTTGAAAGGATCCCAAAGCCCAGTGTATTT960.10831180260173975No Hit
GTAGTGGGGAATGTATCCATGCATTTGGATACTTCTGAAAATAATGACTAGA960.10831180260173975No Hit
ACAGTAGGGGTATAAAGCAAATTATCACAAAATCAGATGTGCAGTTACATGT960.10831180260173975No Hit
ACGCTCGGGTAGATAGGAATCGGCCATATGACGAAATGAGATCAATAGGAAA960.10831180260173975No Hit
GCACGGAGGGAATGAGTACCTGGATGAAACTACACTAGTGGTGGTGGAGATG960.10831180260173975No Hit
TTATTAGGGACACGCTATGGTAAACAAACTACATTGTTCGGTAGATATCATT960.10831180260173975No Hit
TGTGCCTGGGATCCCAGTTTTCCGTCTGGGAACAGTACCTCTGCCCCTGATT950.10718355465797164No Hit
GCTTTAGGGACTTGTGAGGACTCACATAAATGCTGTTGTGGCAAAGATTCAG950.10718355465797164No Hit
AGGTGCGGTGGCTAGTAATTACTGCCATTATTGTCTGTGATACAGCAGTGTT950.10718355465797164No Hit
AAAGATGGGAAACATTAAATGTGTCAAGAAATCTTGGCAACAGAGACTCTGC940.1060553067142035No Hit
CGCAGGGGGTCAGTGTGAGAATACTTTTTACCCTAGGACACACAGTTCCCTT940.1060553067142035No Hit
TGGAGTGGGACCTCATAGGATTATTGTGAGAATTAAATTAACTTCACTATAG940.1060553067142035No Hit
GCTTCAGGGCTCTTAAAGGTACTACTACCTATCTAACAAAGCTTGAAGCCCC940.1060553067142035No Hit
TGGCGTTGGGCCGATCTTCTACTGGGTGCCAGACATTTATAGGTACTCAACT940.1060553067142035No Hit
GTACATGGGATGATCATTTCAAATGATGCTAAAAAAGCATTCAACAAAATTT930.10492705877043539No Hit
TGCCACGGGTCCGATCTACTACTGGGAGAAGGGAAACGTGAAGAAGGTGAAG930.10492705877043539No Hit
AGAAATGGGGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTGTA930.10492705877043539No Hit
TATCACAGGGCCCTCTGACGAGGTCTGTAGCGGCCCTTCTCAGAGTGGAACA930.10492705877043539No Hit
ATAATTGGGCTGTATCTCTAATGGATGGGCCATGCATGTTTCCATGCTGACA930.10492705877043539No Hit
TCTGCATGGGCAACGGTGAGCTTAAAATATTCAGTAAACCATGCTATAAACA920.10379881082666727No Hit
GCGACCGGGATTTGGTCAAAACCTGCCAGTGCAGCCTCTTCTTACAGACTAG920.10379881082666727No Hit
GCACAGGGATGTGTTTTAGATTGATTTCCCTGTTTCAGGGAAATCACGGACA920.10379881082666727No Hit
ATTCGGGGGCCACAGCCTATGTGAGTCCAACCGTTCAAGGCAGCAGTAACAT920.10379881082666727No Hit
CTGACAGGGTTTCGGTGACATTAATGAAGAAAATTTCTCATGCCAGGCTTTA920.10379881082666727No Hit
GACTCTGGGATATTATGAGGTGCTGTAAGGCATGGAAACCATGAGACACAAC920.10379881082666727No Hit
TCACACGGGTAGCCTTGGTCATGCTTCCTAGAATCTACTTGTCCAAGTTCTC910.10267056288289914No Hit
TTACTGGGGGAGAATAATTATTTGATTGCTAGATGCAGCTTAACACCTACTG900.10154231493913102No Hit
ACTGTTGGGGACGCTCTTCCGATCTACAATAGGGAACCCTGATTTATGTTCT900.10154231493913102No Hit
ATGGATGGGACAGGATGCCAGCCTGAAGCCAAAAGTAGAATGTAGTGTGGTG900.10154231493913102No Hit
AAGACCGGGGGAAAAAGAACTGCAGCAGGAACTTCAAGAATATGAAGTGGTG900.10154231493913102No Hit
TTATATGGGACTCAAGACATGTGTGGACGAGATGCAGAAGATTACGCTATTT900.10154231493913102No Hit
ATATAGGGACAATGCAGCTTGAGAATGTTGGTATTGGCATTTCTTTAACTGA900.10154231493913102No Hit
GAGCCTGGGTAACAGAGTGAGACCCCCAACTTAAAACAAAAATGAGTAAGAC900.10154231493913102No Hit
TCACTTGGGATGGGGAATGATTTAGGAGTGATTTAGATAACCACTGACCCCT900.10154231493913102No Hit
AAGCTCGGGTATCTAAGTGTATCTAAACATAGAAAAGGAAATGCATTGTGCT900.10154231493913102No Hit
CTCTCAGGGATTAGTAACTGTGCTGTTCATTATTTTTAGTAACAGCACATAG900.10154231493913102No Hit
ATTGGTGGGACTACATCAATGGCAGTTTATTTTGTGAACATGATAGACCTAC900.10154231493913102No Hit
CTACGAGGGATACCAGAACGTCTAGTATAATTACAGTCATGCATTGCTTAAC900.10154231493913102No Hit
TCTATTGGGATGACTCAGAAATACCACATTTTTCTTAGTGACCTGTTTTACC900.10154231493913102No Hit
ACGATAGGGAACCCTTTCAACTCTAAAAATTGACCCTATCAACTGGAGAGCC890.1004140669953629No Hit
CTAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA890.1004140669953629No Hit
ACTCGGGGGACCAAGGATTGAAACTTTCAACCCCACCTCTGACTTCCAGCAG890.1004140669953629No Hit
AACTAGGGCTGGGTGAACAATCCTTTGTCATAGTTTCTGCAATCAGAATACA890.1004140669953629No Hit
ATTATAGGGCCTCACCACCTACTTTGTCTCTCTGTCTCTCTCACATACACAC890.1004140669953629No Hit
ATAACGGGGCAGCAATGCATCGAGGTATGAGCTATCAACTTTCTTCCTTTTT890.1004140669953629No Hit
AATGGGGATAAGCGCCGGCTATGCCCCTGTATTGGATTGCCACACGGCTCAC890.1004140669953629No Hit
CGAAAAGGGCTGCAGTGCAGTTGTGAGGGCAGTTAGCAGCCGCCTCTTCTGC890.1004140669953629No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)