FastQCFastQC Report
Sat 31 Dec 2022
SRR5847733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37062
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC9252.4958178187901354No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT7121.9211051751119745No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3350.9038907776158869No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2530.6826399007069235No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1650.44519993524364576No Hit
GACTACGGGACTGCCACAGCCTTGGTCCAGCCAACCATCTTGTCTCACTTGG1310.3534617667691976No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA1080.291403593977659No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT1050.28330904970050186No Hit
GCCTAGGGATCTTCATTATACTCATGATTATCATCATGATTATAATCATCAG960.2590254168690303No Hit
GCAACGGGAGCAACACGAAGGTTTATTCCAAAAAGAAAAGGGGCTGACAGAC810.2185526954832443No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAACTCGTATGC700.18887269980033458No Hit
AAAATTGGGAAACTGAATCCAGGCAGTGCCATGACCCTGGGTGTCCCTTGGT620.16728724839458203No Hit
CGACCCGGGTACTCTAGAGATTACACCTGTCAAATATTTTAAAAACATGGAA600.16189088554314393No Hit
TGCTTTGGGCAAAACATGCGTGGGAACGCCCACTGTGTGGACTGTGAGACCC580.1564945226917058No Hit
ATGATTGGGAAGCAGAAAAAAGCAGGGGTTGCAATCCTAGTCTCTGATTAAA560.15109815984026767No Hit
CTATGCGGGCTGGTCTTAGTTGTAATTAGGATACAATGGTACAGTGTGTAAT560.15109815984026767No Hit
AATCTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA560.15109815984026767No Hit
TCAAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA560.15109815984026767No Hit
AACCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA560.15109815984026767No Hit
GTCGTAGGGGGCACGCTGGCACTCAGTGCCCTTCTGGTATCATCTGCCAAGA560.15109815984026767No Hit
ACAAGAGGGTACCTCAGGTGAAGATAGGAGCAGCTCCAGTTCCTCTGAAGGT540.1457017969888295No Hit
TATCTAGGGGGGACCCCCAGCACCCAGTCATTCATTAACACACAAAAGACAC540.1457017969888295No Hit
CATGTTGGGGTGGCTTCCCTGGATAGCTTGTATTCACGGAATAGGTTCTCTG540.1457017969888295No Hit
CCATAAGGGATGCAACAACATGGACGAATTTCAAAATAATTATGCGGAGTGA530.14300361556311045No Hit
TACATAGGGGTGATTCAAGATCAAATGAATGAAATGAAGCAAGAAGAGAAGT530.14300361556311045No Hit
TACTGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA510.13760725271167235No Hit
ACATTTGGGGGAAAGAAGCAGCTGATATGCTAAAGGCATTACAAGGAGCCAG510.13760725271167235No Hit
GAGTCAGGGATCTTCAAAGCTGAACTGAATGAGTTTCTTACTCGGGAGCTGG500.13490907128595328No Hit
AATTGTGGGTCCAACCAACACAGATATACCTTTGACCTAGAGGATTTCATGT490.13221088986023422No Hit
CACGCTGGGGGGACCAGGTAGCCGAGGACAGTGAGGCGTGTCTGAGCCACTT490.13221088986023422No Hit
CTTGAAGGGTGACTGTCCTGTTTCCCCCAGGCCACTTTTTTTTTTTTTTTTT480.12951270843451515No Hit
TTTAGCGGGGTATGACTTCAGTATATTCCTGGGAATGCCATAGGATTTTTTG480.12951270843451515No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCG480.12951270843451515No Hit
TCTTTTGGGGGGTGGCGGCGGCCGCGGAGCGGAACAAGGATCCCATCTTGCA480.12951270843451515No Hit
TCACACGGGACACCTGCAAGCAAGCAAGTCCAGTAGACTCTGGTAGAAACTA470.12681452700879609No Hit
GACGAAGGGGCCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAAG470.12681452700879609No Hit
TGCGCTGGGTTAAATCTTTAGTTCTGGTTAAAATCTTTAAAAAATATTTTTC470.12681452700879609No Hit
AAGCCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA470.12681452700879609No Hit
TTAAAAGGGCTTGGAAGCCCTCTTATGAGCGAATTCAGGAAATGAAAGCTAA470.12681452700879609No Hit
TATTGAGGGAAGGAAATACCCTGAAGAGTCATGATTAACTATTGACTATTTT460.124116345583077No Hit
ATCATGGGGAAGTGGAAGTCCTCAGAAATTCTCGGCACACCCTAGAGTATTG460.124116345583077No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCT460.124116345583077No Hit
ATAGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC460.124116345583077No Hit
TTTCGTGGGATCTCTGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTG460.124116345583077No Hit
ACATTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA450.12141816415735794No Hit
AATTTAGGGATGTATTGCTAGTATTATGCCCATTTACAGGTGAGACAACAGT450.12141816415735794No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTAT440.11871998273163886No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACCTCGTATGC440.11871998273163886No Hit
CGCAGGGGGAGGTCGAAAGTTTATTCAAAGGGATAATAACAGAGAACTTCCC430.1160218013059198No Hit
ACCAGGGGCCAAATAAACTTCTTTTCTTTTTAAATTACCCAGTCTCAGATAT430.1160218013059198No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT430.1160218013059198No Hit
ATTGCTGGGGCTGTAGGAATTGGATGCCTTCCCTCTGCGGCTCCTGGAATCA430.1160218013059198No Hit
GCATGAGGAGAGTCGCGGCCGCCAGCTAGGGGCGCGGGAAGGCGGGGCTCGG420.11332361988020076No Hit
ACGCTAGGGTGTGTAAGTTTTCAGATATATAACATATATACCTTTTATTCAA420.11332361988020076No Hit
GCATCTGGATTTGGGGGTGTTTTTTGGCATGGCACCCTCACCTCTCTCCCTG420.11332361988020076No Hit
TAAAATGGGAATCTTAACATTTGTGCAAATGGCAGTGAACAAGGTGGAGTAT420.11332361988020076No Hit
ATGTATGGGAAATAGACCCAAAAAGAAAATAGCATAATGAACTCTAATGTGC410.11062543845448168No Hit
CAAAATGGGGAGAACGAAAGATGGCGGCGCCCGTAAGGCGGACGCTGTTAGG410.11062543845448168No Hit
GAATAAGGGCTGCGAGGGGGGCCGAGGCCTACCACATAGAGGACTCCCACAC410.11062543845448168No Hit
GTACGTGGGCGATCTGCGGACGGGCATTTCTTAAGCCATGATACTTAATGAT410.11062543845448168No Hit
AGATCCGGGTAAAAGCCCAATACCACTGATCATTAGAGAAATGTAAATCAAA410.11062543845448168No Hit
TATTAGGGGGGAGGGAAGGAATGTTACATAACTAAGTGGAGGCCTGGAACTG410.11062543845448168No Hit
TATAGCGGGTGAGGGGTGCTACTAATGTTAGGAGTCAGGTATGCCACTAAAT410.11062543845448168No Hit
CTGAGGGAGTGATTGAAGATCAGATGAATGAAATGAAGCGAGAAGAGAAATT410.11062543845448168No Hit
TTGACTGGGTTTACTTTGCCTGTACAATCGATGTGTATTGGATCCTCATCTC410.11062543845448168No Hit
CTGCCGGGAGATTCAAGTGCAATGGCACGTTTTGGCTCACTGCAACCTCTGC400.10792725702876262No Hit
GGGCCAGGCAGGAAACCCACAGGAAGAAAATGGGGCAGAAGTGGAATGTTTG400.10792725702876262No Hit
CTACATGGGAACCAACTAAACAGTCATAATCTTGGCACTGTTAATAGAAAGT400.10792725702876262No Hit
TACGGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG400.10792725702876262No Hit
CTTCAGAAGGGTGATAGTATTTGGTTTAAAACAGTGGTTTCTAACCACTGGA400.10792725702876262No Hit
CAAGCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC400.10792725702876262No Hit
ATAGTAGGGATCGAGATGTCCCTACAGATGGATCCTGAATACCGTGTGAGAA400.10792725702876262No Hit
TGCAGCGGGTACTTCCTTTAAATGTCCCATGGATGTAGGACAGTGCCATGTT390.10522907560304355No Hit
CTTCACGGGGGCTCACTGCAACCTCCGCCTCCCAGGTACAAGCGATTCTCCT390.10522907560304355No Hit
GCTGCGGGCGATCTTCGGATAGGGATATCAGAAAGATGGCATATCAGATATG390.10522907560304355No Hit
ACAATTGGGCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCC390.10522907560304355No Hit
GGCTGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC390.10522907560304355No Hit
CAAAAGGGGACATAAAAAGAATTTTGTGTAACTGGCAGTTTGAGCAACAATG390.10522907560304355No Hit
GCAAGTGGGCAGATGAGAGCCCCTCTGTGTGCAGCATTTGAAAATGAAATCA390.10522907560304355No Hit
CTAAATGGGACCTCAGCACACATTACAAGGCCCCTCGAATGGTGCTGGCAGC380.10253089417732449No Hit
AGCGTAGGGAGAACCTAGGGGAAATACAAGGCTAGATTTCTGCAAGCCACTG380.10253089417732449No Hit
CTAAGAGGGTAAATAATGAGGACCCTTTCATCAGGGTTTTTGAGTGAGTTTT380.10253089417732449No Hit
TCACATGGGTGGTCTTGTGTCCTATACTTCATGTGTGATGCCACCAGAAATG380.10253089417732449No Hit
GCTTAAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG380.10253089417732449No Hit
ATGACTGGGGGCAGTGCTGGTGGAAAAATCTGTGGTACTGGTGATCTGGTTA380.10253089417732449No Hit
TATACCGGGGATTCCAGAAGCCTCAGATGATTCAGGTTTGGAAGTCAGAAGG380.10253089417732449No Hit
GCGATTGGGTTATTCCTGACATGTAAAAATCAATTTTACGTAGAGTCAACAT380.10253089417732449No Hit
GTAATAGGGGGGGCCTGGTTCCGGGCGTGCGCAGCCGCAGAGCGCCGCAGCC380.10253089417732449No Hit
TCTTGGGAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT380.10253089417732449No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAT380.10253089417732449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)