FastQCFastQC Report
Sat 31 Dec 2022
SRR5847732.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847732.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36905
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC8642.341146186153638No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT7281.9726324346294541No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3340.9050264191843923No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2200.5961251862891207No Hit
GACTACGGGACTGCCACAGCCTTGGTCCAGCCAACCATCTTGTCTCACTTGG1370.37122341146186155No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1360.36851375152418375No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT1020.2763853136431378No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA900.2438693943910039No Hit
GCCTAGGGATCTTCATTATACTCATGATTATCATCATGATTATAATCATCAG890.2411597344533261No Hit
ACAAGAGGGTACCTCAGGTGAAGATAGGAGCAGCTCCAGTTCCTCTGAAGGT720.19509551551280313No Hit
CGACCCGGGTACTCTAGAGATTACACCTGTCAAATATTTTAAAAACATGGAA710.19238585557512533No Hit
ATGATTGGGAAGCAGAAAAAAGCAGGGGTTGCAATCCTAGTCTCTGATTAAA680.18425687576209188No Hit
CTATGCGGGCTGGTCTTAGTTGTAATTAGGATACAATGGTACAGTGTGTAAT660.17883755588673622No Hit
GCAACGGGAGCAACACGAAGGTTTATTCCAAAAAGAAAAGGGGCTGACAGAC590.15986993632299146No Hit
GTAATAGGGGGGGCCTGGTTCCGGGCGTGCGCAGCCGCAGAGCGCCGCAGCC590.15986993632299146No Hit
TAAAATGGGAATCTTAACATTTGTGCAAATGGCAGTGAACAAGGTGGAGTAT590.15986993632299146No Hit
TTAAAAGGGCTTGGAAGCCCTCTTATGAGCGAATTCAGGAAATGAAAGCTAA580.15716027638531363No Hit
TGCTTTGGGCAAAACATGCGTGGGAACGCCCACTGTGTGGACTGTGAGACCC570.15445061644763583No Hit
TCAAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA570.15445061644763583No Hit
CATGTTGGGGTGGCTTCCCTGGATAGCTTGTATTCACGGAATAGGTTCTCTG560.151740956509958No Hit
GCATGAGGAGAGTCGCGGCCGCCAGCTAGGGGCGCGGGAAGGCGGGGCTCGG550.14903129657228018No Hit
TATCTAGGGGGGACCCCCAGCACCCAGTCATTCATTAACACACAAAAGACAC550.14903129657228018No Hit
CACGCTGGGGGGACCAGGTAGCCGAGGACAGTGAGGCGTGTCTGAGCCACTT550.14903129657228018No Hit
TACTGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA540.14632163663460238No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAACTCGTATGC540.14632163663460238No Hit
TCACATGGGATAACCTGAGACTTACTTTCATTTTCTAGGTGCTGGAGAATCT540.14632163663460238No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCT530.14361197669692452No Hit
CTCTAGGGGGGCGTCCCAAGATGGCGTCGTGGCTGCCGGAGACTCTCTTTGA530.14361197669692452No Hit
CTCTGATAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC520.14090231675924672No Hit
TTTAGCGGGGTATGACTTCAGTATATTCCTGGGAATGCCATAGGATTTTTTG520.14090231675924672No Hit
AATTAAACTTAAAAGCTTCTGCTCAGCAAAAGAAATTATCAATAGAGTGAAC510.1381926568215689No Hit
TCACACGGGACACCTGCAAGCAAGCAAGTCCAGTAGACTCTGGTAGAAACTA500.13548299688389107No Hit
GATAAAGGGGCAATTCTGAATGGGAGTTCACTCATGATTTGGCCTGTTATTG500.13548299688389107No Hit
AATTGTGGGTCCAACCAACACAGATATACCTTTGACCTAGAGGATTTCATGT490.13277333694621327No Hit
GTTAAAGGGAGTCTATCGCTGCGGTTGCGAGCGCTGTAGGGAGCCTGTGCTG490.13277333694621327No Hit
AAAATTGGGAAACTGAATCCAGGCAGTGCCATGACCCTGGGTGTCCCTTGGT490.13277333694621327No Hit
GTAGTAGGGGGTCCCCGGAGCGGGGATCACCTCAACTTGACTGTGGTCCCCC490.13277333694621327No Hit
TACATAGGGGTGATTCAAGATCAAATGAATGAAATGAAGCAAGAAGAGAAGT490.13277333694621327No Hit
ATGTATGGGAAATAGACCCAAAAAGAAAATAGCATAATGAACTCTAATGTGC480.1300636770085354No Hit
ATGGCTGAATGAACAATCAGTGAAATAATAGATGGATAGATAAGCGGATGGA480.1300636770085354No Hit
TATTGAGGGAAGGAAATACCCTGAAGAGTCATGATTAACTATTGACTATTTT480.1300636770085354No Hit
AATCTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA480.1300636770085354No Hit
GACTGGGGATAAAACTGAAGGTGGAGAGCAGCCCTTCTCGGAGCGATTACAT470.1273540170708576No Hit
TCTTACGGGTAATTTATTTTGTCGAATTTTTATTATTTGTTTTCCCGCCAAG470.1273540170708576No Hit
CTCTCGGGGCAGAGTGACAAATTCGTTTTATAACACAGGCAACTGTTTGTGT470.1273540170708576No Hit
CTTGAAGGGTGACTGTCCTGTTTCCCCCAGGCCACTTTTTTTTTTTTTTTTT460.1246443571331798No Hit
AAGCAGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC460.1246443571331798No Hit
TATGCTGGGATGCCTGTGTGAAGACAAGCGACCAACCTGCATTCATGCCCTT460.1246443571331798No Hit
TTAAAGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC460.1246443571331798No Hit
TGCGCTGGGTTAAATCTTTAGTTCTGGTTAAAATCTTTAAAAAATATTTTTC460.1246443571331798No Hit
GTCGTAGGGGGCACGCTGGCACTCAGTGCCCTTCTGGTATCATCTGCCAAGA460.1246443571331798No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATG450.12193469719550196No Hit
CCTAACGTGGGGGTGAGGAAGCTCCTCCAATCCTCTGGCTATTTAAGGTTCA450.12193469719550196No Hit
AAGCCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA450.12193469719550196No Hit
TCTTTTGGGGGGTGGCGGCGGCCGCGGAGCGGAACAAGGATCCCATCTTGCA450.12193469719550196No Hit
GCATATGGGTGCTATATCCCTTTAAATAAAAAGAATGTTGACATAAATCCAG440.11922503725782414No Hit
GTAACAGGGTGTGCTTCCTAACAAATTTGAAAGAAAGAGATCTTAGTGTAGC440.11922503725782414No Hit
CGGGCCGGGAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA440.11922503725782414No Hit
ATGAATGGGTGAATGCAGGAAGGAAGTAATGAGTGAGACGTGGAACCGCACG430.11651537732014633No Hit
TTTCGTGGGATCTCTGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTG430.11651537732014633No Hit
CCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACC430.11651537732014633No Hit
GGACACGGGAAGTGGGACCGAATTAAAGAGCTTCTGCATAGCCAAAGAAACT430.11651537732014633No Hit
ATTGCTGGGGCTGTAGGAATTGGATGCCTTCCCTCTGCGGCTCCTGGAATCA430.11651537732014633No Hit
GAATAAGGGCTGCGAGGGGGGCCGAGGCCTACCACATAGAGGACTCCCACAC420.11380571738246849No Hit
AGATCCGGGTAAAAGCCCAATACCACTGATCATTAGAGAAATGTAAATCAAA420.11380571738246849No Hit
TTGATCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC420.11380571738246849No Hit
CTGAGGGAGTGATTGAAGATCAGATGAATGAAATGAAGCGAGAAGAGAAATT420.11380571738246849No Hit
CGTACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC420.11380571738246849No Hit
ACAAAGGGGTCCCCCCTTACATCTTTTCCTCTATTTAGATACAAAATATGTC420.11380571738246849No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCG420.11380571738246849No Hit
TAGACAGGGTGTTCACAAACACCAAAGTAAAGGAAAAATGCAGGATGTTACC420.11380571738246849No Hit
CATCAGGGGATTTAAAGTTCACATAATCATCTGTAAGACATTATGTATTTTG420.11380571738246849No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTAT410.11109605744479067No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACCTCGTATGC410.11109605744479067No Hit
CAAAAGGGGACATAAAAAGAATTTTGTGTAACTGGCAGTTTGAGCAACAATG410.11109605744479067No Hit
TCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA400.10838639750711286No Hit
ACATTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA400.10838639750711286No Hit
CCATAAGGGATGCAACAACATGGACGAATTTCAAAATAATTATGCGGAGTGA400.10838639750711286No Hit
GCGATTGGGAGCTTAAAGCAGTCCCAAGAGGGAAATTTATAGCACTGTTATA400.10838639750711286No Hit
ATAGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC400.10838639750711286No Hit
ATCACTAGGGATAGTTCATTAAGTAACATAGTAGCTAAGAACATGGACTATA400.10838639750711286No Hit
GAAAACGGGTAAGGCTGTGGTGCCTCAGCCTCTGGGCGCGGACTCCAAGGGG390.10567673756943503No Hit
GAGTCAGGGATCTTCAAAGCTGAACTGAATGAGTTTCTTACTCGGGAGCTGG390.10567673756943503No Hit
CGACAGGGGCTGCACCCGCGCTGCATCCACGTCGACACGAGCCAGTGCTGCC390.10567673756943503No Hit
TACTTTGGGGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTA390.10567673756943503No Hit
GCAGTGGGGATTCTTATTTTTAAAATTAATTTTTAGAGATAGTGTCGCTCTG380.10296707763175722No Hit
AAGTCAGGGCATTGAGAGTTGTCTCCAAGGAACCACTTTAAAATCCAAATCA380.10296707763175722No Hit
GGGCCAGGCAGGAAACCCACAGGAAGAAAATGGGGCAGAAGTGGAATGTTTG380.10296707763175722No Hit
TTTCTAGGGGATCTGCATTGGGAAGTGGGGCGATGCTGGCGCTGAGTACGTC380.10296707763175722No Hit
GCTTGTGGGCGATCTTGATGAGGGAATAAAGTAAAATACAGATGACCCTCAA380.10296707763175722No Hit
ATCATGGGGAAGTGGAAGTCCTCAGAAATTCTCGGCACACCCTAGAGTATTG380.10296707763175722No Hit
GGCTGATATTAAGGTCTTTGATTGTCACAATATTCTCAATAAATGAGACATT380.10296707763175722No Hit
GGCCTCGGGGATCTGATTCAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAG380.10296707763175722No Hit
CGTCGCGGGGACACCGGGGACAAGGCAATTCAGTGTGGCCGCCACGTGGATA380.10296707763175722No Hit
CGCTAGGGATTGCTTCATTTGGCAAGGAAGAGTAGATTCATATTTGGCTATT380.10296707763175722No Hit
AACCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA380.10296707763175722No Hit
GAAAGGGGGGTTTCTGTGTGAGCCTGACCCGGTGCACTTCTCAGAAGCAATA380.10296707763175722No Hit
GCAAGTGGGCAGATGAGAGCCCCTCTGTGTGCAGCATTTGAAAATGAAATCA380.10296707763175722No Hit
AACGCGGGGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCTCCTCC370.10025741769407938No Hit
TGCAGCGGGTACTTCCTTTAAATGTCCCATGGATGTAGGACAGTGCCATGTT370.10025741769407938No Hit
AGTAATGGGCTTCCGATCTGGAATAGGGAGAATTTGATGAAGTAGATTGGTT370.10025741769407938No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTC370.10025741769407938No Hit
CAAAATGGGGAGAACGAAAGATGGCGGCGCCCGTAAGGCGGACGCTGTTAGG370.10025741769407938No Hit
GTACGTGGGCGATCTGCGGACGGGCATTTCTTAAGCCATGATACTTAATGAT370.10025741769407938No Hit
GTAGCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA370.10025741769407938No Hit
GGAGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAGA370.10025741769407938No Hit
TGGCCAGGGCATTTAGAAATAACTCAAACAACTCAAGTTTATCACTATGTTT370.10025741769407938No Hit
ATTGCCGGGAAGAAAGTGGATTTGATCCCCAGTCTCATTGCTTAGTAGAATA370.10025741769407938No Hit
TGTTGTGGGAGTGGCTTACTCTACAAATTTTGGTGCTGGCAAATACATAGGC370.10025741769407938No Hit
AAACACGGGCCAGCTAAGCAGCTAAGGTGGGCAGGATCCCAGGAGATGGAGG370.10025741769407938No Hit
ACACGGGATCGGGCAAGCAACTGCAGCAGACCAGGAGAAAAACCCTGAAGGT370.10025741769407938No Hit
AGCACTGGGCGATCTCACTATGGGAGACAAGGAGGCCCTGCAGGAGGGCCGC370.10025741769407938No Hit
TTGACTGGGTTTACTTTGCCTGTACAATCGATGTGTATTGGATCCTCATCTC370.10025741769407938No Hit
GCAATGGGACTATACTTATCTTCAGAAATGCCTGCCTTAGAATTCACTTGGT370.10025741769407938No Hit
CTCCATAGGGGATATGTTAAAGTATAGATAACATTTCACACTTGGATACATA370.10025741769407938No Hit
TCTTGGGAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT370.10025741769407938No Hit
GGCTTTGGGATCTGTGCGTGGGACAGCACTCCAGCCTGGGCAACCCCTGTAT370.10025741769407938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)