FastQCFastQC Report
Sat 31 Dec 2022
SRR5847731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36238
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC8482.340084993653071No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT6491.7909376897179756No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3380.9327225564324741No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2570.7092002869915558No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1320.3642585131629781No Hit
GACTACGGGACTGCCACAGCCTTGGTCCAGCCAACCATCTTGTCTCACTTGG1280.3532203764004636No Hit
GCCTAGGGATCTTCATTATACTCATGATTATCATCATGATTATAATCATCAG1100.3035487609691484No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT1070.2952701583972625No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA1000.2759534190628622No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAACTCGTATGC770.21248413267840388No Hit
ACAAGAGGGTACCTCAGGTGAAGATAGGAGCAGCTCCAGTTCCTCTGAAGGT750.20696506429714664No Hit
GCAACGGGAGCAACACGAAGGTTTATTCCAAAAAGAAAAGGGGCTGACAGAC650.17936972239086044No Hit
CGACCCGGGTACTCTAGAGATTACACCTGTCAAATATTTTAAAAACATGGAA570.15729344886583146No Hit
ATGATTGGGAAGCAGAAAAAAGCAGGGGTTGCAATCCTAGTCTCTGATTAAA560.15453391467520283No Hit
TACGGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG560.15453391467520283No Hit
TCACATGGGTGGTCTTGTGTCCTATACTTCATGTGTGATGCCACCAGAAATG540.14901484629394557No Hit
ATCATGGGGAAGTGGAAGTCCTCAGAAATTCTCGGCACACCCTAGAGTATTG540.14901484629394557No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCG540.14901484629394557No Hit
GCTCTAGGGCAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGC530.14625531210331694No Hit
TATCTAGGGGGGACCCCCAGCACCCAGTCATTCATTAACACACAAAAGACAC520.14349577791268833No Hit
TCTTTTGGGGGGTGGCGGCGGCCGCGGAGCGGAACAAGGATCCCATCTTGCA510.1407362437220597No Hit
AATTGTGGGTCCAACCAACACAGATATACCTTTGACCTAGAGGATTTCATGT500.1379767095314311No Hit
ATGTATGGGAAATAGACCCAAAAAGAAAATAGCATAATGAACTCTAATGTGC500.1379767095314311No Hit
TCAAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA500.1379767095314311No Hit
GTTAAAGGGAGTCTATCGCTGCGGTTGCGAGCGCTGTAGGGAGCCTGTGCTG490.13521717534080246No Hit
GATCCGGGTGAATGCCCATCTGTGGCAAGATACCAGAGTAGGACAGAGCTAT490.13521717534080246No Hit
TCTTGGGAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT490.13521717534080246No Hit
TCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA480.13245764115017386No Hit
TGCTTTGGGCAAAACATGCGTGGGAACGCCCACTGTGTGGACTGTGAGACCC460.12693857276891662No Hit
ACAATTGGGCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCC460.12693857276891662No Hit
GCAAGTGGGCAGATGAGAGCCCCTCTGTGTGCAGCATTTGAAAATGAAATCA460.12693857276891662No Hit
CTATGCGGGCTGGTCTTAGTTGTAATTAGGATACAATGGTACAGTGTGTAAT450.12417903857828798No Hit
CATGTTGGGGTGGCTTCCCTGGATAGCTTGTATTCACGGAATAGGTTCTCTG450.12417903857828798No Hit
AAGCCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA450.12417903857828798No Hit
AACCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA450.12417903857828798No Hit
GCAGTGGGGATTCTTATTTTTAAAATTAATTTTTAGAGATAGTGTCGCTCTG440.12141950438765937No Hit
TCTCCTGGGATTGTAACCCCAAGCAAAATTCCAACAGTATATTTTAAATAAA440.12141950438765937No Hit
GTAATAGGGGGGGCCTGGTTCCGGGCGTGCGCAGCCGCAGAGCGCCGCAGCC440.12141950438765937No Hit
GGAAGTGGGTACTCAGGAGGCTGATGCAGAAGATTGCTTGAGCCCAGGAGTT430.11865997019703074No Hit
TATTGAGGGAAGGAAATACCCTGAAGAGTCATGATTAACTATTGACTATTTT430.11865997019703074No Hit
TCCTTAGGGGTGTGCCAGCGGTGTGGGGAGGACGGGGCAGGACGCTGCAGCT430.11865997019703074No Hit
CAAGCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC430.11865997019703074No Hit
CTTAACGGGGTACTTAATAATTCATAATTTAGCCATGATAGTATCTAAGCTC420.11590043600640212No Hit
GTACGTGGGCGATCTGCGGACGGGCATTTCTTAAGCCATGATACTTAATGAT420.11590043600640212No Hit
TATTAGGGGGGAGGGAAGGAATGTTACATAACTAAGTGGAGGCCTGGAACTG420.11590043600640212No Hit
TTTAGCGGGGTATGACTTCAGTATATTCCTGGGAATGCCATAGGATTTTTTG420.11590043600640212No Hit
CAAAAGGGGACATAAAAAGAATTTTGTGTAACTGGCAGTTTGAGCAACAATG420.11590043600640212No Hit
CACGCTGGGGGGACCAGGTAGCCGAGGACAGTGAGGCGTGTCTGAGCCACTT420.11590043600640212No Hit
TACTGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA410.11314090181577349No Hit
AAAGATGGGCTGCTTTAAAATTAGCATATTTTATAATAAGCTGATTTTAGAT410.11314090181577349No Hit
TATAGCGGGTGAGGGGTGCTACTAATGTTAGGAGTCAGGTATGCCACTAAAT410.11314090181577349No Hit
ACCAGGGGCCAAATAAACTTCTTTTCTTTTTAAATTACCCAGTCTCAGATAT400.11038136762514486No Hit
ACACGGGATCGGGCAAGCAACTGCAGCAGACCAGGAGAAAAACCCTGAAGGT400.11038136762514486No Hit
GCAACTGGGGCTGCCGGGGATCTGGGGGTGGGAGTTGTTTTTTCAAAGCACC400.11038136762514486No Hit
GGACACGGGAAGTGGGACCGAATTAAAGAGCTTCTGCATAGCCAAAGAAACT400.11038136762514486No Hit
GTCGTAGGGGGCACGCTGGCACTCAGTGCCCTTCTGGTATCATCTGCCAAGA400.11038136762514486No Hit
TGCAGCGGGTACTTCCTTTAAATGTCCCATGGATGTAGGACAGTGCCATGTT390.10762183343451626No Hit
TTTGCCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC390.10762183343451626No Hit
CTTCACGGGGGCTCACTGCAACCTCCGCCTCCCAGGTACAAGCGATTCTCCT390.10762183343451626No Hit
GTAGCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA390.10762183343451626No Hit
TGTCTAGGGCAAGTGGCTCATCCCTGACAGCGCCGACACCACGGTGGGCAAC390.10762183343451626No Hit
GCGTTGGGGATAACAAAGCCAGGTTGAGTGGGAAAAAAATTTTGCCAAAAGA390.10762183343451626No Hit
CGTACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC390.10762183343451626No Hit
ACATTTGGGGGAAAGAAGCAGCTGATATGCTAAAGGCATTACAAGGAGCCAG390.10762183343451626No Hit
CTCTTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG390.10762183343451626No Hit
GTAGCTGGGATAGTTAAGTAACTGAATGAGTAGTTTAGGGACATTACAAACC390.10762183343451626No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAT390.10762183343451626No Hit
CCCGGCGGGTGGAATGTGAATGAGCATAGTTATGTTTTTGATTGTGCAATAG380.10486229924388762No Hit
CGCCTTGGGATCGAAGTATATTGATGTATTCTAAAGCTAGTAAACTTCCCTA380.10486229924388762No Hit
TTGATCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC380.10486229924388762No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTAT380.10486229924388762No Hit
TGCCGAGGGAGCCTCCTGGCTCAAGCGATCCTTCCACCTCAGCCTTCGGAGT380.10486229924388762No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT380.10486229924388762No Hit
GAGTCAGGGATCTTCAAAGCTGAACTGAATGAGTTTCTTACTCGGGAGCTGG380.10486229924388762No Hit
GCACATAGGGGTAGAGGCATATTAAACATCCAAGTGGAGATATCAAGGAGAC380.10486229924388762No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG380.10486229924388762No Hit
TACATAGGGGTGATTCAAGATCAAATGAATGAAATGAAGCAAGAAGAGAAGT380.10486229924388762No Hit
CAAAATGGGGAGAACGAAAGATGGCGGCGCCCGTAAGGCGGACGCTGTTAGG370.102102765053259No Hit
ATACCTGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG370.102102765053259No Hit
TTTCGTGGGATCTCTGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTG370.102102765053259No Hit
GTAACAGGGTGTGCTTCCTAACAAATTTGAAAGAAAGAGATCTTAGTGTAGC370.102102765053259No Hit
GCAGTTTGGGCTCAAGCAGTCCTCTTGCCTCAGTCTCCCAAGTAGCTGGGAC370.102102765053259No Hit
GCGATTGGGTTATTCCTGACATGTAAAAATCAATTTTACGTAGAGTCAACAT370.102102765053259No Hit
ATGCTGGGGGAGTGAGGTATGTTTTCTGCACAGCTCCTATCAACAAGAAGCA370.102102765053259No Hit
ATAGTAGGGATCGAGATGTCCCTACAGATGGATCCTGAATACCGTGTGAGAA370.102102765053259No Hit
TAAAATGGGAATCTTAACATTTGTGCAAATGGCAGTGAACAAGGTGGAGTAT370.102102765053259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)