FastQCFastQC Report
Sat 31 Dec 2022
SRR5847730.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847730.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101656
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT7510.7387660344691902No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC5890.5794050523333596No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC4160.4092232627685528No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2660.2616667978279688No Hit
TGACTGGGTGGTTATGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGAG1470.14460533564177225No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1450.14263791610923113No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1450.14263791610923113No Hit
AACAATGGGCAGAGGGAAACTGAGGCCAGCAGAAAGGATGTCACTGGACCCA1440.14165420634296058No Hit
GCAAGTGGGCTGGACGGCGACCTGGCGGGCCGATACTACGCGCTCAAGAGCA1370.13476823797906665No Hit
GTAGGTGGGGGCAACAGGGCCGACTGCAGCTGGAAGATGGCGGCGTCCGTGG1140.11214291335484379No Hit
TTTCGAGGGGAGAGAGGATGGTGTCTCCCATCGAAAGTGCTGAGGATCTTTC1110.10919178405603212No Hit
TCAGTAGGGAGGTGGAGCCTGCTCTTAGAACACACCAGAAGGCTGGAAGGCT1080.10624065475722043No Hit
CGAAGCGGGATCCTCATCCGCAAGTACCAGGCCAACTCGGGCACTGCCATGT1060.10427323522467931No Hit
CTTCGAGGGATAGCAGTAATTCATTTTTATTGCTGAATAAGTATTACATTGC1030.10132210592586763No Hit
ATAGAAGGGATCGTGCCACTGTACTCCAGCCTGGGCGACAGAGTGAGACTCT1020.10033839615959707No Hit
GTAACCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1020.10033839615959707No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)