FastQCFastQC Report
Sat 31 Dec 2022
SRR5847729.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847729.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102762
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT7310.7113524454564918No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC5660.5507872559895681No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC4240.4126038808119733No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2610.2539849360658609No Hit
TGACTGGGTGGTTATGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGAG1420.13818337517759485No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1380.13429088573597242No Hit
GTGCATGGGATGGCCCACAATGCAGAGCATGAGGCTTGCGACCTGCTTATGG1300.12650590685272767No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1300.12650590685272767No Hit
TCTGCAGGGCTCCGCTCACTGCAACCTCTGCCTTCTGGGTTCAAGCGATTCT1300.12650590685272767No Hit
AACAATGGGCAGAGGGAAACTGAGGCCAGCAGAAAGGATGTCACTGGACCCA1270.12358653977151088No Hit
TTCGAGCGGGATAGGATGCAAGAAAAGGAGTGAGGAATGGGAAAATTGGAAG1230.11969405032988849No Hit
GGCCATGGGATAAAACAAAGAAGAATTGGTACCAATTCTACAGAAACTATTC1210.11774780560907729No Hit
GCAAGTGGGCTGGACGGCGACCTGGCGGGCCGATACTACGCGCTCAAGAGCA1200.11677468324867168No Hit
TGCCAAGGGACCAGGAATAAGGCATTCTGGGAAACCCCTGCCTCCATGTGGG1160.1128821938070493No Hit
TCAGTAGGGAGGTGGAGCCTGCTCTTAGAACACACCAGAAGGCTGGAAGGCT1160.1128821938070493No Hit
TTTCGAGGGTCTTGTACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC1120.1089897043654269No Hit
TAATGCGGGGGTGAGTTCTAAGAGGAATAGCTGGGGAAAATCTCCAAGCTGA1100.10704345964461572No Hit
TTAGGCGGATACTAGCTACCGAAGCAAAAACAAAGCCCTAGGAATAAATTTA1080.10509721492380451No Hit
GCTCTAGGGAAATAAGAAGCATGAATAACATTTAAATTCCACAGATTATCAA1050.10217784784258774No Hit
TCAGCTGGGATTTTAGGCATGCACCATCACGCCCGACCAATTTTTGTATTTT1050.10217784784258774No Hit
AGCTTTGGGATAGAAAAGAATAATATTCTGTGGTCTTGGTTGGTGGGCCCAG1040.10120472548218212No Hit
AAAATAGGGCTCTTGAAGTTTGGATGCATATTCTAACAATCTGACCTCCCTT1040.10120472548218212No Hit
ACTTTCGGGCAGCGATTCACAGCTTTCTACGCCAGCCGCCACAGTGGCCGAA1040.10120472548218212No Hit
TGCATAGGGATTGGATGAACCAAGAGATATGTGGGAGAGAGAGAGAAATAAG1040.10120472548218212No Hit
ATAGAAGGGATCGTGCCACTGTACTCCAGCCTGGGCGACAGAGTGAGACTCT1030.10023160312177654No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)