FastQCFastQC Report
Sat 31 Dec 2022
SRR5847728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98642
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT7400.7501875468867216No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC5890.5971087366436203No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3820.3872589769063888No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2450.24837290403682002No Hit
TGACTGGGTGGTTATGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGAG1520.15409257719835365No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1350.13685853895906408No Hit
AACAATGGGCAGAGGGAAACTGAGGCCAGCAGAAAGGATGTCACTGGACCCA1280.1297621702722978No Hit
TTCGAGCGGGATAGGATGCAAGAAAAGGAGTGAGGAATGGGAAAATTGGAAG1220.12367956854078385No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1210.12266580158553152No Hit
GCAAGTGGGCTGGACGGCGACCTGGCGGGCCGATACTACGCGCTCAAGAGCA1150.11658319985401756No Hit
TTTCCAGGGCAGGAAGATGATATTTGCGTCTTTTGCCCACCCCCCTGGCATT1130.1145556659435129No Hit
CTGCCCGGGCCGGCGGTCAGTGCCCCGAGGTGAGGCGGCGGGGGCGGTGCAG1080.10948683116725128No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA1060.1074592972567466No Hit
TGCCAAGGGACCAGGAATAAGGCATTCTGGGAAACCCCTGCCTCCATGTGGG1010.102390462480485No Hit
TTTCGAGGGGAGAGAGGATGGTGTCTCCCATCGAAAGTGCTGAGGATCTTTC1000.10137669552523267No Hit
TAATGCGGGGGTGAGTTCTAAGAGGAATAGCTGGGGAAAATCTCCAAGCTGA990.10036292856998033No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)