FastQCFastQC Report
Sat 31 Dec 2022
SRR5847725.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847725.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100484
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT7340.7304645515703992No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC5550.5523267385852474No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3890.3871263086660563No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2410.23983917837665697No Hit
CATACTGGGTGAGCTGAATAATTTTAGTTTCTTAAATCAACCGCACTCAAAA1460.14529676366386687No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1370.13634011384897096No Hit
AAAATCGGGGATCTCCAGCTTGGAAATGAGACTTCTCATGAGCTTATTCTAA1290.1283786473468413No Hit
GATATCGGGCATCAGTTGGGGTCATACCACTAGTCTCTGTCCTATACCCCAT1210.12041718084471159No Hit
GTGCTAAATGCTCCCTTTGCTTGAAAATTTCCCAGACCACAGTCAGAGTGAC1210.12041718084471159No Hit
TGATTTGGGACTCCAATTTTTAAAAGAATTGTTAGAAATGATTCAAATGCAA1060.10548943115321842No Hit
TACAGCAGGGCATGGCCTTGCTACTTGGATTAGCTCCTTTAAGCCTGAAAAT1030.1025038812149198No Hit
TTTGATGGGGGTCGCGCGGCCGGGTGGCCTCGGTCGGTACCCTGGGCGCGGA1030.1025038812149198No Hit
TATCTCTGGGCCTCTGTATGTTTTGCATTCTCATAGCTTAGCTCCCACTTAT1010.10051351458938737No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)