FastQCFastQC Report
Sat 31 Dec 2022
SRR5847715.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847715.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32300
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC5861.8142414860681115No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT5341.653250773993808No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC2970.9195046439628483No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2460.761609907120743No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1100.3405572755417957No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT1050.32507739938080493No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA730.22600619195046442No Hit
GCTCGCGGGATTTCTAGTGTAATTCAGTGCAAAAGGAGGAGGAACAGTTTTA650.20123839009287925No Hit
CAACAAGGGATGTCATCCCAGGTGATACTTCATTTATATTCAAATTTTGCTT620.19195046439628483No Hit
AGACTAGGGGGGCGAGGACTCGGCTGTGTTGATGGAGAGACTGTGCAAGTAC610.18885448916408668No Hit
CACTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA570.17647058823529413No Hit
GATAGTGGGATTAAGCCCTTATTCCTAAGAGGCTAAAGAAACTCTTAGAAAA540.16718266253869968No Hit
AACATAGGGTATACTAAGGTCCATTTCTTACAAATGTACTATCATAATGTAT530.16408668730650153No Hit
GTGTAAGGGATATTCAAAAGAAAATAAATCATTCTACCAAAAAGATGCATGC530.16408668730650153No Hit
TAGCTTGGGGAGGGGAGCAGTCACAGGGATCACCCCCATTATTTGCTGAGAA500.15479876160990713No Hit
AGCTGGGGGACGCTCTTCAGATCTAAGGCGGGTGAGGATGTTGCTTTGTTGC490.15170278637770898No Hit
TGTATGGGGGCCGCCACGGAAAAGCAGTCTTCTGCACGGGACGGGGAGCGGC490.15170278637770898No Hit
GTGCACGGGATCTGGGGAAACCTCAGTGTCAAACCAACTGGAATGGTTGATT480.14860681114551083No Hit
TATATAGGGAGTTCAAGTGATTCCCCTGCCTCAGCCTCGCGAGTAGCTAGGA470.1455108359133127No Hit
TCTATAGGGCGGGTGCACCCAGTTTCCACCATGATTAAGGGTCTTTACGGAA460.14241486068111456No Hit
AGCAAGGGGACAAATCACCAAAGAACAAATTCCAGCCGGCAAATAAATTCAA460.14241486068111456No Hit
GGGCCTGGGTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGG460.14241486068111456No Hit
GCTGTTGGGCTAAACCTCTGGGGGTATGAACGGGTAGATGAAATTATTTGGG460.14241486068111456No Hit
TGCTGTGGGCAGTCTTGGGTATTGGGAAGACTTGGCCTGTCTAAGCTCACGA460.14241486068111456No Hit
GGACTGGGTAGCTTACAAATGGCTATAAATTATTTGCATAGGAGGTGCACTT450.1393188854489164No Hit
GCTACGGGGCAGAAATTCCAAGGTGTGCGACTGCTGCGACAAGACCCCATCG450.1393188854489164No Hit
TTGGTGCGGGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCACGAGAAAG440.13622291021671826No Hit
AAGCGCGGGTAAGATGGACAAAAATAAAAAAGAATGAACAAAACCTTCAAGG440.13622291021671826No Hit
AGTACAGGGATATAGTGATTCGGAAGATGATTCAGAATTTGATGAAGTAGAT430.1331269349845201No Hit
ATTTACGGGAAGTAATCTTCTACTATTAACAAGTTCAGTGGTTTTATTTTTA430.1331269349845201No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAACTCGTATGC430.1331269349845201No Hit
AAAGTAGGGGGGAACTGCAATTCAAGATGAGATTTGGGTGGGGACACAGCCA410.12693498452012383No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT410.12693498452012383No Hit
TTAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC400.12383900928792571No Hit
TCTGTTGGGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGG400.12383900928792571No Hit
ACTTAAGGGTGGTTTCTGTATATGTGAAGGCTGTGGATTTCTGTATGTTAAT390.12074303405572755No Hit
CTTGCGGGGCGCCTGCTGTTTCGCTTCTACGACATCAGCTCTGGCTGCATCC390.12074303405572755No Hit
GAACCTGGGAGAGAACATGTAGGTACCACTGCCACTGCCATTGACATGTTCA390.12074303405572755No Hit
TGCGTGGGGAGGAATGGGGAGTTTAGTTTAAAAGACAACACTAGATACTTTT380.1176470588235294No Hit
GGCGATGGGACCTCGTGCTGCCCATGCCTGTGCAACTGTCGGAAATAGAGGC380.1176470588235294No Hit
GGCCCTGGGAGCCAATTAGTAAATGTTTTAGAGCTTACGAGAATAAACATTG380.1176470588235294No Hit
GTAAAAGGGAAAGGATTATTGTTTACAAAGGTGTAATTATTGAATGGTCATC380.1176470588235294No Hit
GCCACTGGGGTACTTTGCATGGTACTTCATTGGCATTTGATAACCTATAAGA380.1176470588235294No Hit
CACGGTCGGGTTGGATTGCCTTATCAGACTTCAGGCTCAGAACATACAGCAA380.1176470588235294No Hit
TTATTGGGGCGCTGGTGATGGTATTGCTGCTGTAACAGCCTTACATTTTAGG370.11455108359133127No Hit
TGCTTAGGGCTTTGGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT370.11455108359133127No Hit
ACTGGCTGGGCCAGTGCAGACAGCATCAGCTCTGAGGTTCATCATCTTCTTA370.11455108359133127No Hit
AAAAGTGGGGGCCAGGGTGCTCCCTGGGATTCACAGTCCCTTCCCCTCTTTG370.11455108359133127No Hit
GCTCCTGGGTTGCGGTGGGTATATACTGTTCATGTACATCCACAAATACACC370.11455108359133127No Hit
TGTGGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA370.11455108359133127No Hit
ATTAAAGGGGTGGAGAAACATTTCCTAACTCATTCTATGAGGTCAGAATTAC370.11455108359133127No Hit
TTTACTGGGTATCACAAATAATCTGTTATCAATAAAGTACTGGAATTTATCA360.11145510835913312No Hit
GATATTGGGGGAGCAAGGACTCGGGGCCCGCGCGGCGCCTCTCGGGGCCGGG360.11145510835913312No Hit
AGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGGCACAGAC360.11145510835913312No Hit
AATGCTGGGATGTACTTTTAAAATATGTATGTGTTTAATCAGTAATAGAAAT360.11145510835913312No Hit
ACAGAAGGGAAGGTATTGAATAAGGTATTCTTGTCAGTTTAGAAATGGACTG360.11145510835913312No Hit
GTTAATGGGATTCCTATTTCACCTTCCTCCATCTCCAGTTTTGCTTTCATAC350.10835913312693499No Hit
CGGACCGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT350.10835913312693499No Hit
AGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG350.10835913312693499No Hit
GTCCAAGGGAGACCTATGCTAGTTCCTGTCATCTATTCGCTACTAAATGCAG350.10835913312693499No Hit
TAACTGCGGGACAAAAACAAACAAAAAGAACCATGTTACTAAATAGCCTGGG350.10835913312693499No Hit
TTGTTTGGGTGAAGTCATCTGGATTCCAATCCAAGTTCCCTATTAGGTTAAG350.10835913312693499No Hit
AGACTAGGGGGGCTTCTCGGCACGGGGGCAGGGGCTGGAGCTGGGCCTCCCC350.10835913312693499No Hit
GTCTGAGGGGGTTCATCTTCTGTATATAACCAATCTCTCAATCATGCTGGCC350.10835913312693499No Hit
GTCTGCGGGGAGGCCTCCCCAGCCATGCTGAACTGTGAGTCAATTGAACCTC350.10835913312693499No Hit
ATAGCGGGGGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTC340.10526315789473684No Hit
GGCCTGGGATGATAAAGACATTAAGTTAACAAACATATAAGAAATGTATGCA340.10526315789473684No Hit
CCTTGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC340.10526315789473684No Hit
CAACTAGGGATAGAGTGCTAGAGCTCTTACGCCCCTCCCCTTCTTCCCAACT340.10526315789473684No Hit
TTACTTGGGTCCAGCACCCAATCTAGCTGGAGCTGTTGAATTCAATGATGTG340.10526315789473684No Hit
AGTAGCGGGGCACACTTGTTATTAGGCTAGGAGTAGCCAAGCAGGTCTTGGG340.10526315789473684No Hit
GCTACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC340.10526315789473684No Hit
TATTCAGGGGACTCTCGCGTGCGCCCGTGAGCGCCACGAACTCGCTCCCACG340.10526315789473684No Hit
CATGTAGGGTGAATGCAGTTTCTGCGTTTGACTATATGCAGTATGCCTTCTA330.10216718266253871No Hit
TCTTCTGGGATTTAACATAGACTCACAACATAGCTAATCATGACAAAAGACT330.10216718266253871No Hit
ACCTGTGGGAAGATAGGAAACCCACTCAATTCCTTGGGTTACACTTTACATT330.10216718266253871No Hit
TTAAGTGGGGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAG330.10216718266253871No Hit
AACCAAGGGAAGACAAGGAATCCGGATTTGATCTGATAGGAACAGGAAATTC330.10216718266253871No Hit
CTGTTAGGGGGATTGGCCCTGATCATAATAGAGAACCCTGAGGAAGTTTACT330.10216718266253871No Hit
AGCGGTGGGAGTAGGAAATGGGAGTGTAGAGATCACATTCTGGGCATTACTG330.10216718266253871No Hit
AATTAAGGGATGAAAGAAAATATCAGATTTGCACTTTAAATGAGCTTAATTG330.10216718266253871No Hit
TGTGTTGGGTCATCTGGGAAGTGGGGAGCACCTCTGCCCGGCCACCCCGTCT330.10216718266253871No Hit
TAACAGGGGAAACTTCATAGATATGGACATTCCCACTGGGGAGATTCCCTGT330.10216718266253871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)