FastQCFastQC Report
Sat 31 Dec 2022
SRR5847714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32566
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC5691.7472210280660811No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT4951.5199901738008967No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC2640.8106614260271449No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA1940.5957133206411596No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1030.3162807836393785No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT850.2610084136829823No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA710.21801879260578516No Hit
AGCAAGGGGACAAATCACCAAAGAACAAATTCCAGCCGGCAAATAAATTCAA690.2118774181661856No Hit
GCTCGCGGGATTTCTAGTGTAATTCAGTGCAAAAGGAGGAGGAACAGTTTTA670.205736043726586No Hit
TGTATGGGGGCCGCCACGGAAAAGCAGTCTTCTGCACGGGACGGGGAGCGGC580.17809985874838788No Hit
AGCTGGGGGACGCTCTTCAGATCTAAGGCGGGTGAGGATGTTGCTTTGTTGC560.1719584843087883No Hit
TGCTTAGGGCTTTGGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT560.1719584843087883No Hit
GTGCACGGGATCTGGGGAAACCTCAGTGTCAAACCAACTGGAATGGTTGATT560.1719584843087883No Hit
CACTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA550.16888779708898852No Hit
TTTCCCGGGGCTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAA550.16888779708898852No Hit
AGACTAGGGGGGCGAGGACTCGGCTGTGTTGATGGAGAGACTGTGCAAGTAC530.16274642264938893No Hit
CTGTTAGGGGGATTGGCCCTGATCATAATAGAGAACCCTGAGGAAGTTTACT520.15967573542958913No Hit
CGGACCGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT490.15046367377018977No Hit
CAACAAGGGATGTCATCCCAGGTGATACTTCATTTATATTCAAATTTTGCTT480.14739298655039No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAACTCGTATGC480.14739298655039No Hit
AAGCGCGGGTAAGATGGACAAAAATAAAAAAGAATGAACAAAACCTTCAAGG480.14739298655039No Hit
GCATTAGGGTCTACAGCTAAATTTTGTTTACAATTAGGCTATTTTTTAATAT460.1412516121107904No Hit
AGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG460.1412516121107904No Hit
TTAGGTGGGCTAGGCGCGGGTTTGTTGAAGATGATCATTTGATATGTTTGAT460.1412516121107904No Hit
AGCGAGGGGTATGGAAGGCCAGTGAAGAAGCAAAGGAAGACATGAAAATTGA460.1412516121107904No Hit
GCTCCTGGGTTGCGGTGGGTATATACTGTTCATGTACATCCACAAATACACC460.1412516121107904No Hit
TACACAGGGGGAACAGATTTTATTGACTGCCTCGAAATCTTTTTGAACGATT460.1412516121107904No Hit
TTAACGGGGGGAGTGCAATGGCGTGATCTCGGCTCACCGCAACCTCTGCCTC450.1381809248909906No Hit
AACATAGGGTATACTAAGGTCCATTTCTTACAAATGTACTATCATAATGTAT450.1381809248909906No Hit
TCTATAGGGCGGGTGCACCCAGTTTCCACCATGATTAAGGGTCTTTACGGAA440.1351102376711908No Hit
CGTAGGGGGATAAATTTAAGCATATAAAAATCACACCAAGTATCTTTTCTGA440.1351102376711908No Hit
GGGCCTGGGTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGG440.1351102376711908No Hit
TACTGAGGGGCTTGGAGATGGAGTTTTTTTTTTCCACTTAGGATATAAAAAC440.1351102376711908No Hit
TTAAGTGGGGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAG430.13203955045139104No Hit
TTAATGGGACCTGGCATTCGACCATCATCACCTGTCACAGTTACAGCTCTTG430.13203955045139104No Hit
GATATTGGGGGAGCAAGGACTCGGGGCCCGCGCGGCGCCTCTCGGGGCCGGG430.13203955045139104No Hit
GCTGTTGGGCTAAACCTCTGGGGGTATGAACGGGTAGATGAAATTATTTGGG430.13203955045139104No Hit
TTAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC420.12896886323159124No Hit
GGACTGGGTAGCTTACAAATGGCTATAAATTATTTGCATAGGAGGTGCACTT420.12896886323159124No Hit
CTTGCGGGGCGCCTGCTGTTTCGCTTCTACGACATCAGCTCTGGCTGCATCC420.12896886323159124No Hit
AGTACAGGGATATAGTGATTCGGAAGATGATTCAGAATTTGATGAAGTAGAT410.12589817601179143No Hit
GTGTAAGGGATATTCAAAAGAAAATAAATCATTCTACCAAAAAGATGCATGC410.12589817601179143No Hit
ACTGGCTGGGCCAGTGCAGACAGCATCAGCTCTGAGGTTCATCATCTTCTTA410.12589817601179143No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCT410.12589817601179143No Hit
GCCACTGGGGTACTTTGCATGGTACTTCATTGGCATTTGATAACCTATAAGA410.12589817601179143No Hit
TAGCTTGGGGAGGGGAGCAGTCACAGGGATCACCCCCATTATTTGCTGAGAA410.12589817601179143No Hit
TAATCGGGGTACTCTGGGGCCAGGTAAAAATTTCATCAGTTTTTGTTTAAAC410.12589817601179143No Hit
TGGTGATGGGTTATTGCTGCATCACATATAGACTCATTAGGTGGAAATTATG400.12282748879199165No Hit
AAAGTAGGGGGGAACTGCAATTCAAGATGAGATTTGGGTGGGGACACAGCCA400.12282748879199165No Hit
TGCTGTGGGCAGTCTTGGGTATTGGGAAGACTTGGCCTGTCTAAGCTCACGA400.12282748879199165No Hit
ATTTTAGGGCTTCACTGTCAACCACCCAGATAACCAGGTGATCGTGCAGTCC400.12282748879199165No Hit
TATATAGGGAGTTCAAGTGATTCCCCTGCCTCAGCCTCGCGAGTAGCTAGGA390.11975680157219186No Hit
GGCTCTGGGGGGAAACTGAGTCTCCTTCATATCAATGAGTAAAGGTCTTTGG390.11975680157219186No Hit
GGCCCTGGGAGCCAATTAGTAAATGTTTTAGAGCTTACGAGAATAAACATTG390.11975680157219186No Hit
TGGCAAGGGGAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCG390.11975680157219186No Hit
GGTCATGGGGAGGCTTCAATGAGCCATAATTGCACCACTGCACTGCAGCCTG390.11975680157219186No Hit
TCTGGCAGGGAACATACTTCTAAAAAGTTGCCAAGCATAGTATTCTTTTATT390.11975680157219186No Hit
AGACTAGGGGGGCTTCTCGGCACGGGGGCAGGGGCTGGAGCTGGGCCTCCCC390.11975680157219186No Hit
ATTTACGGGAAGTAATCTTCTACTATTAACAAGTTCAGTGGTTTTATTTTTA390.11975680157219186No Hit
CTATAGGGAGGGGATACAAAGACAAAACATGTTTGTGCCAGCAAAGACCTAA390.11975680157219186No Hit
TGGCTGGGACCTGTTGGCCACCGCTGTTTGGTATATGCAAAAATGTGTAGTT380.11668611435239205No Hit
ACTTGAGGGAACTTGAGCATTCTTTTGGATTCTTTGGTTTACATTTGTGCAG380.11668611435239205No Hit
GGCCTGGGATGATAAAGACATTAAGTTAACAAACATATAAGAAATGTATGCA380.11668611435239205No Hit
CAAGTTGGGCCTGAAAGATAGTGCCACCAGCAAAACTTTTGAAAAGAATGTT380.11668611435239205No Hit
GATGTTGGGAGATAAATGGGGAAGATTTTATCTTCCTGGAGTCCTAAGCCAC380.11668611435239205No Hit
TTGAATGGGGGAGGCCCTGGAGCGCGGCGGTGATGGCGGGGCCGGTGAAGGA380.11668611435239205No Hit
TTTGCAGGGGGAAAAGAAAAGGTCAAAGTATTCTTGTTTGCTGATGACATTA370.11361542713259228No Hit
TTGGTGCGGGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCACGAGAAAG370.11361542713259228No Hit
CCGTCACGGGAGGATAAGGAGCTGAAAGATTAACTTGGATCTTCTAATGGTC370.11361542713259228No Hit
AGTAGCGGGGCACACTTGTTATTAGGCTAGGAGTAGCCAAGCAGGTCTTGGG370.11361542713259228No Hit
TATGATGGGTTTTGTGAAGTGTCTGTTTAAAATGTTTGCCAATTTAAAACTT360.11054473991279248No Hit
CTCGAGGGGTAAGGACGCAGACTTGTACACGTCCAGGGTGATGCTCAGTAGT360.11054473991279248No Hit
TATTATGGGAACAAGGACAACCGGGTGTCCCTGGCGGAAGCCAAGTCCGTGT360.11054473991279248No Hit
GTCTGCGGGGAGGCCTCCCCAGCCATGCTGAACTGTGAGTCAATTGAACCTC360.11054473991279248No Hit
GTTAATGGGATTCCTATTTCACCTTCCTCCATCTCCAGTTTTGCTTTCATAC350.1074740526929927No Hit
AGAGATAGGGGTGACAGAGCCAGTAAAGATGTTTTTCAGAGTGAGGAATTCC350.1074740526929927No Hit
TAGAGGGATCTGGCTACATTACTGAATTGTTTATTATTTAATAGCTTTTTAG350.1074740526929927No Hit
TGTGGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA350.1074740526929927No Hit
TTTCCTGGGAGAAAAGGACATAACCAAAAAAGAAAACTGCAGACCAATATCC350.1074740526929927No Hit
TATTCAGGGGACTCTCGCGTGCGCCCGTGAGCGCCACGAACTCGCTCCCACG350.1074740526929927No Hit
TCAACAGGGGAATCTGCAGCAGAGATTTACCCGAGCGTGTTGCGGCAGCGGC340.1044033654731929No Hit
AAACATGGGCACTGAAAGACGTTTGGATTATTTTCAGTTTGGGATAATACTA340.1044033654731929No Hit
TGCATAGGGTGCTTTGGTCTTTACTAACTTAAATCATAGTAATAACAACAAC340.1044033654731929No Hit
ATCCCAGGGATCCCCGGGTCTTTATCATTCAGTTTGTTCTGTGCACCTGAGG340.1044033654731929No Hit
ATCTAAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC340.1044033654731929No Hit
AATGGCGGAATCTATTTAGCAAACGTTTTTGGCATAAAAGGAATGATAATTA340.1044033654731929No Hit
TAACAGGGGAAACTTCATAGATATGGACATTCCCACTGGGGAGATTCCCTGT340.1044033654731929No Hit
AAGTCAGGGGCTGGAGTGGATCACGAAGGGTAGGAAGCTGAGAAAGGTGGAA340.1044033654731929No Hit
CAATGCGGGACTAGGAGTGTGCCCTTTTAATCTTTACTAGTTATTGTGAGAT340.1044033654731929No Hit
CACGGTCGGGTTGGATTGCCTTATCAGACTTCAGGCTCAGAACATACAGCAA340.1044033654731929No Hit
AGCCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA340.1044033654731929No Hit
AGTCAGGGGCAGGTATGAGCTACTGCACCTGGTCCCCTTCTCATACACTTTT340.1044033654731929No Hit
ATAGCGGGGGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTC330.10133267825339311No Hit
AGCTTGGGACTGTGGCAATGCAGGCAAGAGGCTGAGGAACAAGGTGCCAGCC330.10133267825339311No Hit
GAAAGAGGGTAACTTCAGGCATACTGAGGGAGCCTTTTTGTTTTCTGTTTTC330.10133267825339311No Hit
CAACACGGGCTAGTTTGACCTTTGAAATTTGCATGTGACCTCATCTAGCTAT330.10133267825339311No Hit
AATCTAGGGAGGCACTAGGAAGCAACAGCGATGGAACAAAGAGGAAGGAGTA330.10133267825339311No Hit
AAGTAGGGGAGCTAGAAGACGAGGCCAAAGCTGCCCGAGCCCGAGCTCTGGC330.10133267825339311No Hit
GACCATCGGGTAGCTTTCTTCAAAAGAGGTGATTCCAAAGAGTCACCCACTC330.10133267825339311No Hit
GTTCTTGGGAAAGAGACAGAATGTGTGAGGATTGGGAATTAACCCATAGGGG330.10133267825339311No Hit
TAAGGTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT330.10133267825339311No Hit
TTGTCCGGGTCTACAAGTGGGGTCTATCTATTATTATGTCTAATACCACATT330.10133267825339311No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAT330.10133267825339311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)