FastQCFastQC Report
Sat 31 Dec 2022
SRR5847713.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847713.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32434
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC5681.7512486896466672No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT4971.5323426034408336No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3040.9372880310784979No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2180.6721341801812912No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1050.3237343528396127No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT1000.30831843127582165No Hit
GCTCGCGGGATTTCTAGTGTAATTCAGTGCAAAAGGAGGAGGAACAGTTTTA810.24973792933341557No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA800.24665474502065735No Hit
TTAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC660.20349016464204228No Hit
AGCGAGGGGTATGGAAGGCCAGTGAAGAAGCAAAGGAAGACATGAAAATTGA570.17574150582721834No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCAACTCGTATGC560.17265832151446014No Hit
CTGTTAGGGGGATTGGCCCTGATCATAATAGAGAACCCTGAGGAAGTTTACT530.1634087685761855No Hit
AAGTCAGGGGCTGGAGTGGATCACGAAGGGTAGGAAGCTGAGAAAGGTGGAA530.1634087685761855No Hit
GATAGTGGGATTAAGCCCTTATTCCTAAGAGGCTAAAGAAACTCTTAGAAAA520.16032558426342727No Hit
GCTGTTGGGCTAAACCTCTGGGGGTATGAACGGGTAGATGAAATTATTTGGG520.16032558426342727No Hit
GGACTGGGTAGCTTACAAATGGCTATAAATTATTTGCATAGGAGGTGCACTT510.15724239995066905No Hit
TGCTGTGGGCAGTCTTGGGTATTGGGAAGACTTGGCCTGTCTAAGCTCACGA510.15724239995066905No Hit
AGCTGGGGGACGCTCTTCAGATCTAAGGCGGGTGAGGATGTTGCTTTGTTGC490.15107603132515263No Hit
GCCACTGGGGTACTTTGCATGGTACTTCATTGGCATTTGATAACCTATAAGA490.15107603132515263No Hit
CACTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA470.1449096626996362No Hit
AGCAAGGGGACAAATCACCAAAGAACAAATTCCAGCCGGCAAATAAATTCAA470.1449096626996362No Hit
ATCAATGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT470.1449096626996362No Hit
ACTTAAGGGTGGTTTCTGTATATGTGAAGGCTGTGGATTTCTGTATGTTAAT470.1449096626996362No Hit
AAGCGCGGGTAAGATGGACAAAAATAAAAAAGAATGAACAAAACCTTCAAGG470.1449096626996362No Hit
GGCTCTGGGGGGAAACTGAGTCTCCTTCATATCAATGAGTAAAGGTCTTTGG460.14182647838687795No Hit
TCTATAGGGCGGGTGCACCCAGTTTCCACCATGATTAAGGGTCTTTACGGAA450.13874329407411976No Hit
GAGCAGGGGTCAGCATAATAAAAGTGAATTAATTCTATATTAAATTAATAAA450.13874329407411976No Hit
ATTTACGGGAAGTAATCTTCTACTATTAACAAGTTCAGTGGTTTTATTTTTA450.13874329407411976No Hit
TGCTTAGGGCTTTGGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT440.13566010976136153No Hit
GTGCACGGGATCTGGGGAAACCTCAGTGTCAAACCAACTGGAATGGTTGATT440.13566010976136153No Hit
TGTGTTGGGTCATCTGGGAAGTGGGGAGCACCTCTGCCCGGCCACCCCGTCT440.13566010976136153No Hit
TGGCTGGGACCTGTTGGCCACCGCTGTTTGGTATATGCAAAAATGTGTAGTT430.13257692544860333No Hit
AAAGTAGGGGGGAACTGCAATTCAAGATGAGATTTGGGTGGGGACACAGCCA420.12949374113584508No Hit
TAACTGCGGGACAAAAACAAACAAAAAGAACCATGTTACTAAATAGCCTGGG420.12949374113584508No Hit
AGCCTGGGCAACAAGAGGGAAACTCCGTCTCCAAAGAAAAAAAAATGAATAG420.12949374113584508No Hit
TAATCGGGGTACTCTGGGGCCAGGTAAAAATTTCATCAGTTTTTGTTTAAAC420.12949374113584508No Hit
TTGGTGCGGGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCACGAGAAAG400.12332737251032867No Hit
GACTTAGGGGATAACAGTGTTAAATATGTGTGCATAAAGTCTTAAATGAAAA400.12332737251032867No Hit
GCTACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC400.12332737251032867No Hit
GAACCTGGGAGAGAACATGTAGGTACCACTGCCACTGCCATTGACATGTTCA400.12332737251032867No Hit
CGGACCGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT390.12024418819757046No Hit
CAACAAGGGATGTCATCCCAGGTGATACTTCATTTATATTCAAATTTTGCTT390.12024418819757046No Hit
AGACTAGGGGGGCTTCTCGGCACGGGGGCAGGGGCTGGAGCTGGGCCTCCCC390.12024418819757046No Hit
GTCTGCGGGGAGGCCTCCCCAGCCATGCTGAACTGTGAGTCAATTGAACCTC390.12024418819757046No Hit
CATGTAGGGTGAATGCAGTTTCTGCGTTTGACTATATGCAGTATGCCTTCTA380.11716100388481224No Hit
GGGCCTGGGTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGG380.11716100388481224No Hit
TATACTGGGAGAGGGTAGGAAGAATGTGGTGTGTGTGTGTTCTCATAAAGCA380.11716100388481224No Hit
AGCCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA380.11716100388481224No Hit
AAACATGGGCACTGAAAGACGTTTGGATTATTTTCAGTTTGGGATAATACTA370.11407781957205401No Hit
TCTTCTGGGATTTAACATAGACTCACAACATAGCTAATCATGACAAAAGACT370.11407781957205401No Hit
GGCCCTGGGAGCCAATTAGTAAATGTTTTAGAGCTTACGAGAATAAACATTG370.11407781957205401No Hit
TCGTACGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT360.1109946352592958No Hit
AGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG360.1109946352592958No Hit
TGTATGGGGGCCGCCACGGAAAAGCAGTCTTCTGCACGGGACGGGGAGCGGC360.1109946352592958No Hit
GCTAGGGCGCTGGGGGACTGGCTACTACTGCCTGAGCCCATTGGTCCATTGC360.1109946352592958No Hit
GCCCGCGGGGACACTAATGGGAGTTCCCGAGATGCTCGGGTTTGAGCAGGGA360.1109946352592958No Hit
TATTATGGGAACAAGGACAACCGGGTGTCCCTGGCGGAAGCCAAGTCCGTGT360.1109946352592958No Hit
AGTCAGGGGCAGGTATGAGCTACTGCACCTGGTCCCCTTCTCATACACTTTT360.1109946352592958No Hit
TATATAGGGAGTTCAAGTGATTCCCCTGCCTCAGCCTCGCGAGTAGCTAGGA350.10791145094653759No Hit
AATCTAGGGAGGCACTAGGAAGCAACAGCGATGGAACAAAGAGGAAGGAGTA350.10791145094653759No Hit
AGACTAGGGGGGCGAGGACTCGGCTGTGTTGATGGAGAGACTGTGCAAGTAC350.10791145094653759No Hit
TCTACGGGGGACTGCAAAAACTAGGAGCTCGCATTCATTTCCCAAGTGTGAC350.10791145094653759No Hit
AAAAGTGGGGGCCAGGGTGCTCCCTGGGATTCACAGTCCCTTCCCCTCTTTG350.10791145094653759No Hit
GCGACTGGGAGTATAAACTTGTTGTTTAGTAAACGTGTATTATTATTAATAA350.10791145094653759No Hit
TTGCTTGGGAGAAGAGTATGTGTTTGCTGCGCTGAACCTGTACACAGACATC340.10482826663377938No Hit
TGCATAGGGTGCTTTGGTCTTTACTAACTTAAATCATAGTAATAACAACAAC340.10482826663377938No Hit
CTTCATGGGGGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAA340.10482826663377938No Hit
CCAGCAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC340.10482826663377938No Hit
GCGCATGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTG340.10482826663377938No Hit
GTGCCTGGGATGAATGAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTT340.10482826663377938No Hit
GATGTTGGGAGATAAATGGGGAAGATTTTATCTTCCTGGAGTCCTAAGCCAC340.10482826663377938No Hit
TCGCTAGGGTATTTCTTGATTAGAGGTTTGGGCATAACCAGATTAAAGTGAA340.10482826663377938No Hit
ACTGGCTGGGCCAGTGCAGACAGCATCAGCTCTGAGGTTCATCATCTTCTTA340.10482826663377938No Hit
TTACTTGGGTCCAGCACCCAATCTAGCTGGAGCTGTTGAATTCAATGATGTG340.10482826663377938No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCT340.10482826663377938No Hit
GGTCCTGGGGTTTTTGATATTTGATAATGAAAAAAATCTTTGCTTGTTTATT340.10482826663377938No Hit
TGCAAAGGGTTATAAAATCATAAAATAATTGAATTTCTGTGCTGAAAAGGAC340.10482826663377938No Hit
TTTGCAGGGGGAAAAGAAAAGGTCAAAGTATTCTTGTTTGCTGATGACATTA330.10174508232102114No Hit
CGAGCCGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG330.10174508232102114No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGT330.10174508232102114No Hit
TTAGGTGGGCTAGGCGCGGGTTTGTTGAAGATGATCATTTGATATGTTTGAT330.10174508232102114No Hit
CACCAAGGGTATAAGAAAACCATTACAAAGTTGGGTTTGTATTAGTTTTTTG330.10174508232102114No Hit
AATTAAGGGATGAAAGAAAATATCAGATTTGCACTTTAAATGAGCTTAATTG330.10174508232102114No Hit
TTGCCGGGAATAAAGAGAGACACTACAAAGAGGAATTTTACAGCTGGGCTGC330.10174508232102114No Hit
TACACAGGGGGAACAGATTTTATTGACTGCCTCGAAATCTTTTTGAACGATT330.10174508232102114No Hit
AGTAGCGGGGCACACTTGTTATTAGGCTAGGAGTAGCCAAGCAGGTCTTGGG330.10174508232102114No Hit
CACGGTCGGGTTGGATTGCCTTATCAGACTTCAGGCTCAGAACATACAGCAA330.10174508232102114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)