FastQCFastQC Report
Thu 19 Jan 2023
SRR5847709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155263
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT11310.7284414187539852No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC11090.7142719128188945No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC5140.3310511841198483No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA4130.26600027050874964No Hit
TTCTGAGGGGGAGGCCCTGCAGGAGGGCCGCACCGCCACCCTCCGGTACCCT1970.126881484964222No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1810.11657638973870144No Hit
TACGCAGGGCTGACATGGGGGGGATCCTGAAAAGAAGTGAACTTTTACTTTT1730.11142384212594116No Hit
TTTTGTGGGGGAGGAGATTCAGATCAAAGAGCCAGCCGAGAAGCAAAAATTC1610.10369502070680071No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAG206.276282E-446.0270731
AACCGGT206.276282E-446.0270731
GTCGCGG206.276282E-446.0270732
ACCGGTG206.276282E-446.0270732
GCGACTG206.276282E-446.0270731
TAAAGCG351.0072654E-746.0270731
GACAACG206.276282E-446.0270731
GCACGAG206.276282E-446.0270731
TTCGTAG206.276282E-446.0270731
CGACATG405.527909E-946.0270731
CGACACG206.276282E-446.0270731
CCTAACG206.276282E-446.0270731
ATTCCGG206.276282E-446.0270732
TACGCAG206.276282E-446.0270731
TCAGCCG206.276282E-446.0270731
TGACACG206.276282E-446.0270731
AAACCGG206.276282E-446.0270732
AGACCGG206.276282E-446.0270732
AACGAGG206.276282E-446.0270732
CGTACTG206.276282E-446.0270731
Produced by FastQC (version 0.11.5)