FastQCFastQC Report
Sat 31 Dec 2022
SRR5847704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73239
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC8021.0950449896912846No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT7931.0827564548942503No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3470.4737912860634362No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2600.3550021163587706No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1410.19252037848687176No Hit
CCTCTTGGGTGAGAAATAGTGTTTGCCCTAAACCTTTGGGAAAAAAATACAG1160.15838555960622072No Hit
AACTTAGGGCTCCGCCCTTGGCTGAGACTGGCGCTCCTCGCCGCTTCCGGCG1150.15702016685099468No Hit
AACCGCGGGCATAACATCCACAGGACTCTATGTTTGCAAAAGCCAGCTTAGC1130.15428938134054263No Hit
ACTTTGGGGGTGTTATATTTACAAGTATTTTGAATTCCCTGTTACACATTTT1090.14882781031963843No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA1090.14882781031963843No Hit
TGTTTTGGGATGGGACACAGCAAAAGCAGTACTGAGGGAATTTTATAGCTAT1070.14609702480918638No Hit
CACAAATGGCTGCCAAACTGGAACGTTTGCGAGAGAAGGATAAGCACATTGA1040.14200084654350822No Hit
CAACCAGGGACTGAGAAGGGCCTGGTAACTGTGACATCCCAATAAATCTGAA1040.14200084654350822No Hit
TACAGTGGGGGGATTACAGGTGTGAGCCACCATGCCTGGGCTAAACTCTTTA1020.13927006103305617No Hit
GTGCCTGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1020.13927006103305617No Hit
ATGCTGGGGCAGGAGAGTTGCTTGAGCTCGGGGGGCGGAGGTTGCGTTGAGC1010.13790466827783013No Hit
ACCTAGGGGACGGGATAGCATTCATGAAGTGAATGGAAAGTTGGAAAGTCTC1000.1365392755226041No Hit
ATGCACGGGACTGCAACCTCTCGGATTCAAGGGATTTTCCTGTCTCAGCCTC970.13244309725692596No Hit
AGCGCAGGGCTATACAAATACAGAAAACAGAATAAATAAATAATTTCATTAA960.13107770450169992No Hit
GGCGGTGGGGGCGGAAGGCTGGGAGGGCTGCGCGGGCTGCGCGTCGCCATGG960.13107770450169992No Hit
TTAAGCGGGGGTTTTAATTCTTGGGACTCGGCTAGTATCTCTTAATAGCTTC960.13107770450169992No Hit
GATATCGGGGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGC950.12971231174647388No Hit
AACACGGGTGCCTCAGCAAGTTTTACTTCAATATTAATGGGCAAGATGAATA950.12971231174647388No Hit
TCTCATGGGGATCTTTTCAAGTTTTTGTCTCATGGTATTTTTTCCTCAATGC950.12971231174647388No Hit
CGTACAGGAACTCAGGATTAAGAAACTCACTCAAATCCACACAACTACATGG940.12834691899124784No Hit
GCTCCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA930.1269815262360218No Hit
TCGCCTGGGTGCTCAAGGTCAGTGTCATCTTTGTGTTTAGCTCATCCAAAGG930.1269815262360218No Hit
AGGTTGGGGCATCAGCTTGTCTGCAGGGGATTTCTGGAGGTTTGGGGGCTTG910.12425074072556971No Hit
TTTTGGGGATACTGGCATGAAACACTTTGAATGTAAGGAGTGTAAAAAAACC900.12288534797034367No Hit
GTTCAGGGAGCAAGAACTTGAGAGAAAAACACATCTGTCTCTGCAATGCAAA900.12288534797034367No Hit
TAAACAGGGAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGA900.12288534797034367No Hit
ATTCTGGGGTTGCATAATTCTTTATTACAAAAGACAAAAACTTCTACCCACC890.12151995521511763No Hit
GCAACTGGGCTACAACACAAAAGGCAAAAAATTCTGCTTGTTTTCATTGAAG890.12151995521511763No Hit
CTAAATGGGTAGTTATGTTAGGAATAGAGCTGGGTGTATTAGGAAGAGATTC880.12015456245989158No Hit
AATTATGGGTAACAGACAAGAAACCAGGACATGTGCCATAACACATACATTA880.12015456245989158No Hit
CTTGCTGGGAAGGGAAGGAAAAATGTAAGGTTTAACAGTAAGCACTTGCATT880.12015456245989158No Hit
ATGCTTGGGAGGAACCCAGGAGCCACGGCCTGCTGTTGCTTCAGCCTGGCCT880.12015456245989158No Hit
AAGCTAGGGAATTAAGTTTGTCATCTTCTATGGGCACAGTTAATGGCACTCC880.12015456245989158No Hit
GGTGTGGGAGTTTAACAATAGATCATGTATCAGATTTAATGACAATAAATTT880.12015456245989158No Hit
GGATAAGGGAGGTCCAACGCATAGTCACTGTTTTCACTAACAAATACTCTAA870.11878916970466553No Hit
GATTTTGGGAAGTACACTCATTTTCCCCTAAGTACTTCTAGAAGTCACCAGT870.11878916970466553No Hit
CCTAAGGGGTAATCGCTTGAAGAACTTAAACCTTCTGGATGAATGATTTCTA870.11878916970466553No Hit
TGAACTGGGGTGAACAGCCGTTAAAATCCCAGTTGATTTCTGCAGTTACGGG870.11878916970466553No Hit
TGAAAGGGGACCGGAAAAATCATTGATGTATTTGTGATCCTTAATAATGACT860.1174237769494395No Hit
TTGACCGGGAGTAATTATAATATGTAAGCTATTTGTTATGTCTACAAAGCAT860.1174237769494395No Hit
AGTAAAGGGGAAATCACAAAAGAGCATAGCGGTTATATCAGACCAAATAGAT860.1174237769494395No Hit
GGCATAGGGGAAATTTTAAATAAGTATGATTAATATACTAAAGGCTCTAATG860.1174237769494395No Hit
AGACAAGGGACATCTACCACACCCTGGGCTGTTTGTCAAACATGCCTGGTTT850.11605838419421347No Hit
TACTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC850.11605838419421347No Hit
TTAAAAGGGGTACCCTCTGCCTCTACAGTATTATGACATTTCCTAATAAAAC850.11605838419421347No Hit
TCTATAGGGATCTGATTTTATCTACTGGCACTTTTTTCTTTTCCCAGTTAAG840.11469299143898742No Hit
GACATAGGGGCCTCTCTGAGAATATGCACAAAATGAGACTTTATAAATATTG840.11469299143898742No Hit
GTATATGGGATGGCAACTTGGCAACTGCATGAGCTCGTACTAATTATGTTTC840.11469299143898742No Hit
ATAAGCGGGATGGGCAAAGCACTTAACATGTCTGCTATGGTTTGAATGTGTC840.11469299143898742No Hit
GTAACCGGGGCTTTTCATCTAAGTTACTAAGGTTCTTGTAGCTAAGATTGTA840.11469299143898742No Hit
TTAAATGGGCACCAGCACTGGGTTTTTGTTTTTTGTTTGTTTTTCCGCTTAA830.11332759868376138No Hit
AAAAAAGGGCCTTTGAAGGATGCCCTGTGTAGATTATTCAGTGCCACAAATT830.11332759868376138No Hit
AGACATGGGAGATGGAAAAATTCTCAAGAAAAATAGTATTTTGTAGTAATTA830.11332759868376138No Hit
GTTTGAGGGAGAAGCTCTCCGGGCCCTCAGGGGCTTAGATCCCACTTGAGTC820.11196220592853534No Hit
GCCACTGGGTGATAGGAAAATCATGTTTATCTTTTCAAAAATATCTTACCCA820.11196220592853534No Hit
AGTGCTGGGATTACAGGTGTGAGCCATCGCATCTGGCCTACATTTCTATATC820.11196220592853534No Hit
AATGCAGGAGTAAAGAAACAGGTTGTTCACTTGCTGAGGTGCAAAAATTCTT820.11196220592853534No Hit
GGTACTGGGTGGGAGGTAATTGAATCATGGGGGTGGTTACCTCCATGCTATT820.11196220592853534No Hit
GAAGGGCGATCTATGTCGGGAAGACAAGTTTCCCAACAGCTAGGCTAAGAAC810.11059681317330931No Hit
TGCTCTGGGTATTCCAAATAAAACTTGTATCTTCAGGCTTATGTTACTTGTG810.11059681317330931No Hit
TTCACGGGAGGCCCCTGAGGAGCGATGACGGAATATAAGCTGGTGGTGGTGG800.10923142041808327No Hit
ACTGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC800.10923142041808327No Hit
TTCGCAGGGATCTCCAGATTATCATCTCCTTGCTATTCCTGCCCTCTTCTAA800.10923142041808327No Hit
GGTTATGGGCTGTGTGTGCTGCTTGCTCCTGTTCTGTTCGCTTGCTCCCGTT800.10923142041808327No Hit
TACGGCGGGCTCTCCAGGGGTCTGGAAGCCCTGCTGGTTTTGGGAGTTCCCC800.10923142041808327No Hit
AAAGCGGGGCGATCTGATGTCGGGGATCTCTCGTGGGGGCACATCTGAAAGA800.10923142041808327No Hit
TCGAGCGGGTCTCATTTAAGATTCTCTTTTCATATTGTCCATATTATAAATG800.10923142041808327No Hit
TGGCACGGGAAGGACTTTTCTTTTCAAGATACTCCATTTATCATTACAGAAA790.10786602766285722No Hit
CACTCTGGGTGCTAAATGCTCAACAAGAAGATGATGAATTTGTGTGTCAGAT790.10786602766285722No Hit
GCAGAAGGGGTGCTGATCTGGACCATCTTTGTCTTGCCTTTTCACGGTAGTG790.10786602766285722No Hit
AGAATAGGGGGAAGGTGCTGTAAAGAGTCACAGCAGTTGCAGCCCTGAGAAG790.10786602766285722No Hit
AGTCAGGGGCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGAC780.10650063490763118No Hit
TTTGCGGGGATTGTGATTTTGTCTTCCTTAAAGATTGGAATATGAATAAAAA780.10650063490763118No Hit
AACTCAGGGCGGCATTGGTGTGGTGATCAAGATCGGATCTGCGAACTCTGGT780.10650063490763118No Hit
CTGTGAGGGCATAAGGAACCATGCTGTAACCTATGGTAGCAGCAGCTGGGCT780.10650063490763118No Hit
ATCGCAGGGATTTGTGAATTTGTTGTTCAGCTTTTCATTCAAACAAAAAATA780.10650063490763118No Hit
GTGCTGGGATTACAGGTGTGAGCCACCATGCCTGGGCTAAACTCTTTAAGTC780.10650063490763118No Hit
GCCATGGGGTCAGAACAAATGCAAGCCAGCACCAGCACACCACTGCCTCTTC780.10650063490763118No Hit
AATTATGGGTACAAAGCTGGCTGCTGTCCTGACCAAGCAAGTTCTCTTATCA780.10650063490763118No Hit
GCCTCCAGCACATGCACTAAAATTTAAACTCCATGCCTGGACAAAATCAATG780.10650063490763118No Hit
AAGCCTGGGCTATGTAGTTGGAACACAAACTGAACAAAGCAAACAAAAATCC780.10650063490763118No Hit
GGGTGTGGGACACACACCAAGATGCAGATCACAAACACAGAAAAAGAAATTG770.10513524215240513No Hit
GGCTCTGGGGTGGTTTTGATTTGCATTTCTCTGATGGGTCTATTTCTTTAAA770.10513524215240513No Hit
TTCGAGGGGACCATCCCCCAGCTGGGCATTATGAGAAAAGCAAGATGACTAT770.10513524215240513No Hit
ACGCCGGATTTAAAACAGTATGGAAATACGTAATGTGTGAAGTGAAAATGCA770.10513524215240513No Hit
TATGCTGGGTAGTTTATGGGTTTGGAAACTTGCATGAAAATATTTTAGCCCC770.10513524215240513No Hit
AGTAGTGGGCAAAGTTTCCAAATTCTAATGTGTGCTTGAAAATTCAAATTTT770.10513524215240513No Hit
TTTTCAGGGCATCTCTCTCATGAACTGTATTCATTTTCAAAAAAATTGATTT770.10513524215240513No Hit
CGCATCGGGTGGAAATGGTTTTTGGCTATCATTGCTTTAAACTCACCTCTAA770.10513524215240513No Hit
TGCAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.1037698493971791No Hit
TTGCCTGGGTGCCACCACACCCAGCTAATTTTTTGTATTTTTAGTAGAGACA760.1037698493971791No Hit
GCACCAGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC760.1037698493971791No Hit
ATATCCGGGATTAAAGTCATATCCCCCAACTCAGACTAAAAATGAGGTCATT760.1037698493971791No Hit
CCAGGGGGAGGCGGGTGGATTAATTATTCAGCATCCTCTCCCTTTGTAACTA760.1037698493971791No Hit
TGATGGGGGGCTGGGCACAGTGGCTCACGCCTGTACTCCCATCACTTGGGAG760.1037698493971791No Hit
AGCCATGGGGGCCAGTGTGGCTGGAATATGGTATGTATCAATGGTATTTTTA760.1037698493971791No Hit
ACTCGTGGGAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTT760.1037698493971791No Hit
TTGCTTGGGTACCTGTGCCAGAGAGCTAGAAAGAAGGTCATAAAGGGTTAAA760.1037698493971791No Hit
AGCCATGGATGTGCATCAAGATTCCCTCCTCCCTTTTGGAATTACCAATAGA750.10240445664195306No Hit
GGCAACGGAGGCTGTATGAACGAGGTGACGCATCCAAAGAAGACATTGACAC750.10240445664195306No Hit
ACGTCGGGGTCCTTAGCGCCTGTCTGCTCTCCTCTAACTAGGACCCAGGGCC750.10240445664195306No Hit
AGACGGGGGTCTTTCCGCGGGGTTGGTAACTGAGGTATAAAATAACCAAAGC750.10240445664195306No Hit
GTGCTTGGTGGAGCCTCAACTAGTCTTTCCTAAGCACTGAAATAGAAGAGCC750.10240445664195306No Hit
GTGTAAGGGGTTTGCTGGCAATTTCCCTGATATGAACAAACTCATACAAAGC740.10103906388672702No Hit
GCACGAGGGCTTAGCTCGGGCTCTAACAATTTCTACAGATCAGGAATTCAAG740.10103906388672702No Hit
GATCGTTGGGATAGATTGTTCTGCTGAAAGTTGGGGTCTAAAACCAATCTGC740.10103906388672702No Hit
CCGTCAGGGGGTGCTGAGCCAAGGTGGATGATGTCGAGGTCTCTGAAGGCAA740.10103906388672702No Hit
GTAGTAGGGATGCTGCTGGCGTCTAAGTGTTTGGGAAGCAACAAAGAGCGAG740.10103906388672702No Hit
CAAGTTGGGATACATTCTTTAGGAAAAGACCAGTGCCCTCAGTCTGAGCAAC740.10103906388672702No Hit
ATCGTGGGGGAATGTATTAAAATTCTCTACTTCCTTGTTACATGGTCTTTCC740.10103906388672702No Hit
GTTTTAGGGTCTTGAGGTGGGTACTAACAGACCGCAACCTCAACACCACCTT740.10103906388672702No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)