FastQCFastQC Report
Sat 31 Dec 2022
SRR5847686.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847686.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81650
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGGGTGAATCTCGTATGCCGTCT12001.4696876913655847No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAATCTCGTATGC11991.46846295162278No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGA1760.21555419473361911No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAA1290.15799142682180037No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAAACTCGTATGC1220.1494182486221678No Hit
TACACAGGGCATAGGTAATATGTGGAATCTGTTTCCAAGTATCAAGTCTTCT1020.12492345376607472No Hit
GCCATGGGGAGATTTAACAGGTTAAGGTTGAATATATCTTATCTTTAATGGT990.12124923453766075No Hit
TTCATAGGGACAGACGGTCGGACTGACAGACGTGCAGACGGACAGAAGAGCA980.12002449479485608No Hit
ATAATAGGGGAAGGAAATATGTATAAAACAAGCAGGATTATCTTTAATGAAA980.12002449479485608No Hit
ATGGTGGGGCTTTAAACCATCACCTCGGCCAAGGAGTATGTTGAAAAAGAAA980.12002449479485608No Hit
TGGTACGGGCAGTCGGGTTGATTTCCTCATCTTCTATTTGATGGGCTAACTG960.11757501530924679No Hit
GGTAGGGGATGTAATTGTCAGGTAATTTCAGAATGTTGAAAATTATTCAGTG950.11635027556644213No Hit
CACCTAGGGGAATGCAGAGCCAAAGCATAAAGATGGAAATAAAAGAATTGCA950.11635027556644213No Hit
ATTACTGGGCTTTACTGCCAGTGATTACAAAGTGGCTTCTGCTGAGTACCAC950.11635027556644213No Hit
AGTAACGGGATATTAGATGATATTATTCACTTGTCATTTTGTTTCTTTATCA950.11635027556644213No Hit
CAGTGTGGGAACTATGTAAGTGTTTGGAATCATTTCACAGCAACATAACAGG920.11267605633802817No Hit
AGCAATGGGGAGTCAGACGCTGTGGTACTTGGCATTTAACAACTAGAATTCA920.11267605633802817No Hit
TCGCATGGGATTTGGAGTTGAAATTAGCAAATTGCATTTAAAGTTGAGAGTA910.11145131659522352No Hit
CTAACGTAGGGTGAACCACTGGGTGGGAAACTTTTGGAAGAATACACCCAGC910.11145131659522352No Hit
AGGCAGGGGTGTCCCAGCTAGTTCACTCCTTTCGCCGTGCGTGGTGGAGGCT900.11022657685241885No Hit
ATTACTGGGGATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAA900.11022657685241885No Hit
GCTCATGGGGTTGAAAAAAAGGATGCAAAATAAAAAGCTGCCTAAGGTGAAA900.11022657685241885No Hit
GGATATGGGGAATTTCGAAAGAAATAATTGGCAAGATAATGAGAAAAGAAAA890.1090018371096142No Hit
GCGTAAGGGAGTTAAAGAAAGGAAAAGAAAACAAAAAACGAATGAAAATAAC880.10777709736680956No Hit
TGACAAGGGTAAGGAATGCTGATTTCCTCAGTTCCATTTTGAGGAATGGGGA880.10777709736680956No Hit
CAATTAGGGTTACTGGAATTACAAAACCTATTTTTCCTTTAAATTTCAGCTT880.10777709736680956No Hit
CAAGAAGGGTGGAAATAAAAATTATCATGGGGAAATGTATAGGCTATTTCAT870.1065523576240049No Hit
TATGCTGGGAAGGAGCGCATTGATACTAGAAATGAGTTGGAAAGCTATGCCT870.1065523576240049No Hit
TCTGCGGGCACAACTAAAGTTAATATTTGTTTATGCCATTAAATATTTGTTT870.1065523576240049No Hit
AGTACAGGGAGGTTCAAATTCACACATAACAATATTAACTTTAAATGTAAAT870.1065523576240049No Hit
TCGTTTGGGTGTGCAATTCATAATAAAAGTGAAGTTCAGAAATGTTGCAAAT860.10532761788120025No Hit
GGGCATGGGGAGAAGCAGCATGTAGCAGGCTGCTGGGTAAAACTAACCTTTT860.10532761788120025No Hit
TAAAACGGGCAAGGAGGGGATTGCATTACAAATTCTGGTTATGACAGCTCCA860.10532761788120025No Hit
TGTATTGGGGATCTTATATAGGGACTGACCTTTGATCTAGTTTGACCTTTGA850.10410287813839558No Hit
GTGCTCGGGTTATAAGTGGCATTTTGTTATACATATTTTCATGAGAAGCTGA850.10410287813839558No Hit
AACTGTGGGCCTGGTGGAACCCACTTTCATGTTCTTATCATTGTTTTGATCT850.10410287813839558No Hit
CTTCGCGGGGAATGTAAGTGGATAAACTCTCTAATCAAAAAATATACAGTGG840.10287813839559093No Hit
GTGTACGGGCTTCTAGCATCCTTCATCCTTCAGGTACCAGCCATCCAGACAG840.10287813839559093No Hit
TTTGGCGGGATCTGGCTACAGTGTGGATAACAGATTGGAGGGGGCAAGAATG840.10287813839559093No Hit
GTATAAGGGCTGATTTTTCAATTTAACATCTTCCACTTAGCTTTTTTCATAA840.10287813839559093No Hit
CTGGATTTGGGCATGGCACACGCATTTCCTGAATGTACAAAGAAATGCTGGC830.10165339865278629No Hit
CGTTTGGGGGGAAATCCTAAAGGCCGCGGAGTCGGCGGTGTTGTAGGTAGCG830.10165339865278629No Hit
CGTGTCGGGTCTGCACAGCAAAAGAAACTATGATCAAAGTGAACAGGCAACC830.10165339865278629No Hit
AAAGGCGGGACCACATTCTTTGTAGCTTCAACACTTTAGGACTAAGGCATGC830.10165339865278629No Hit
GACGCGGGATGGTCTTGTTTGTTGACATGACAAATTAACATTCTTATAGTTA820.10042865890998164No Hit
ACTACAGGGATTTTAAAGAAGGAGGAACTGACACCCATAGGAGTTAAATGTC820.10042865890998164No Hit
ACGTGGGGGAGCCCTCAGATAACTTTCTCATTCTTCCAAAATCAGGCTGGGA820.10042865890998164No Hit
TGTAATGGGCCTCACCCTCTGCACCACTAACCAAGACCTTGTCCTCTTGCCT820.10042865890998164No Hit
TCTAATGGGGTGAGAGTGTAGGGCAGATGGAAGGAAGTGAGGTTAAAGGAGT820.10042865890998164No Hit
GTTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC820.10042865890998164No Hit
TCGCTTGGGCACTGGCCCTTGTGGGAGGAAAAATACTTTTTTAACTTGCTTT820.10042865890998164No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)