FastQCFastQC Report
Sat 31 Dec 2022
SRR5847680.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847680.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115231
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGGGTGAATCTCGTATGCCGTCT10230.8877819336810406No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAATCTCGTATGC9740.8452586543551648No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGA1840.15967925297879912No Hit
GACACTGGGATCTCATTGTAGTTTTAATTTGCATTTCTCTGATGACTAGTGA1800.15620796487056435No Hit
CGAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.13538023622115578No Hit
TTCACGGGGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGC1480.12843766000468623No Hit
ACTTTTGGGATATCTTTTACATCTATTTTTCCCAGTCTGTGGCTTGCCTTTT1460.12670201595056885No Hit
TAAACCGGGGTCTCTACAAAAATAAAAGTTAAAAAATATAAAGTTTTAAAAA1460.12670201595056885No Hit
TTGTTTGGGAGCAGCTCCGGCGGCAGCAACAGCGACTACGAGGGATGGCGGC1420.1232307278423341No Hit
AGACAGCGGGATAGCATATCAAACTGACTTTAGAAAAAAACAAACGAGAAAT1410.12236290581527541No Hit
TAGTACGGGTCCTGTTCTAATTTTGCAGTGCAACACAGATATCTGTTTGAAG1410.12236290581527541No Hit
CTCAATGGGAGCCTGGGTGACAGAGTGAGACCCTATCTCAAAACATAAATTT1400.1214950837882167No Hit
ACGACAGGGGAAGCAGCACATTAGTAACCTGACAAGATTTCTTTTTCCCTTT1390.12062726176115801No Hit
GCGAGGGGAATGAAACATTTTGCATAATGAGTGGCTGGATTCAATGGTTTTT1370.11889161770704064No Hit
TCTGTTGGGGAGAGAAACAAATACCATACAAAGATGCTCCAGTACATCTGGC1360.11802379567998195No Hit
ACACAAACATTTAGACCCTAGTAGTCAGTTCGCTTGTTGTTATTCACATGAC1340.11628815162586456No Hit
CACGACGGGTATAGGAAATCTGAATCAATGGGTTTGAATAAAAAAGAGCTTC1320.11455250757174719No Hit
AAAAGTGGGAACATTGATTCTCTGTTGTACTTTTTTGTAACTAATTCTGTGA1320.11455250757174719No Hit
CTAGCAGTGGGAATTGTACCCATAAAAATGGTTAAAATGGTAAATTTTGTTA1310.1136846855446885No Hit
TTCGGGGGGAGAAGACTTCACTGGGACTTACGTCAGTGAGCTTAACATCTTT1300.1128168635176298No Hit
GCCACGGGGAATAAATTAGAATAGAAATTAGATTTAAAAAGCTGAGAGGCCT1300.1128168635176298No Hit
GCTTTCGGGCGCAAACTTTGGGGCTTAAAACAATACAAGTTTATTATCTTAT1290.1119490414905711No Hit
AGACAGGGGATCCCTTTGTATTATAATAGTGACATTTCTAGAAAAGATGCCA1280.11108121946351243No Hit
ACGGCTGGGATAAAGGAAAAGCAAAATCTGTATAACATTATTACTACTTGAA1270.11021339743645374No Hit
ACGCCGGGTATGTTTTTCAAAATTGACCAAATAAAGCAAAAGAGATGAAAAA1270.11021339743645374No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAA1260.10934557540939503No Hit
GTCTCTGGGATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC1260.10934557540939503No Hit
TCTACAGGGATAATTTGCTAGTGAGCTAAAACAATATAATTTATGTAAATCT1250.10847775338233634No Hit
CTGTGTGGACTGGGAGTCATGAATGTCTTTCCAATAATTCAGGGAATTCTAG1250.10847775338233634No Hit
TACTGCGGGAACCCAAGTCTTGAGCAATGTTTTTCTCAAAAAGCTGCTATCC1250.10847775338233634No Hit
ATAATGGGGTGGAAGTGATAGTAAATATGTCCCATGACAGAATAAGGAGTGA1250.10847775338233634No Hit
GATGAAGGGGAAGAAAACAGAAGGGAAACCCTTCAGGATATGGGTCTAGCGA1250.10847775338233634No Hit
ATTTTCGGGAAACTTAGATAATTAAATGTTACCATTTAAAATACATATCTCT1240.10760993135527767No Hit
AAGGAGGGGCAGGGGAAGACCAAAGTTTGCAACTCGAACTGCTTTCGTCCAT1230.10674210932821897No Hit
GCCCATGGGAAACCTAAACCAGATCCATGTTTTTCTTTGCAATTTAGTGCTC1230.10674210932821897No Hit
TAGACTGGAAGAAAGTGGTTTGTAAATTATGTTCCATTTCATAAATAGACAC1230.10674210932821897No Hit
TGCAAAGGGGTGGTATCTTCTGCCATTAAAGTGGAAAGCTGAAGGAAACTTC1220.10587428730116027No Hit
TTACGAGGGGAGTTTACAGAAAGATATGGTGCTAAAACTTACAAAATACTGC1210.10500646527410158No Hit
ATATTTGGGGCTACCATACAATCCAGCAATGCCACTACTGGCTGTTTATCTA1210.10500646527410158No Hit
ATTCGTGGGCCAAGGTGGACTTGAAAACCTCGAACTTTCAAGAAAGTATTTT1200.10413864324704289No Hit
ACAACCGGGCGCGTTAAGACTTTTAAAGGAAATAGAATCGTGATTAAGAAAT1200.10413864324704289No Hit
CGTAATGGGGGAAGGAACTGGGCAGTCCCACCAGAGCAGAAAGAAAGGAGGC1200.10413864324704289No Hit
CAAAAGGGGACTGCTCCGGGTGTCGGCTCCGGCGGGACGGGCGGTTCTGCGT1200.10413864324704289No Hit
GTTACAGGGATCTGCAGTGCTAAGAACCGTGGACCAGGAAGTGCAATTTTGG1200.10413864324704289No Hit
ACAGATGGGATGTTCAAGAGAAAGCTATGAAACTTCATAATGCTTCTAATTC1190.10327082121998421No Hit
TATTGCGGGATTTCAGTGTCAAATCTCATAGTGTTTGATTGTCTCTCAGTAT1190.10327082121998421No Hit
TTATTAGGGGGATGCAGTGCTCGCTGTTGCTGAATAGGCGATCACAACGTGC1190.10327082121998421No Hit
TTGAGCGGGATCTCATCTTAGTGGACAACCCTGATTGCTAGTGAAGATCAAT1190.10327082121998421No Hit
CATTAGGGGATGGTTGGTCCTTTTATTTCGGAGCCTTCTTTTTCATCATCGC1190.10327082121998421No Hit
CGTTCCGGGGAGACCTTTACTCTGGCAATAGGAATTCACATTTCAAAAGCTG1180.10240299919292552No Hit
TAGTAGAGGGATCGGTTTTTCAAACGAATTCAACATCTGTATTCCTCACCCA1170.10153517716586682No Hit
TTAACAGGGGAGCTATTCCAGTGTTGCAGTAGAATTTATTCCCATTCGGACT1170.10153517716586682No Hit
GTAGAGGGATGGTCTACTTAGGTTGACCCATGTGAAATTGCCAGTATTTAAA1170.10153517716586682No Hit
TCGGCCTTGGCCTTGTCGGCGGCGGACAGGCCGGCGGCGATCTTCGCGGCAC1160.10066735513880813No Hit
CTATCGGGGCACATTGGATTATCTTTTGACCACCTCTGAGAACCATATGGGG1160.10066735513880813No Hit
ACACCGGGACTGGTAGGTGATTTTTATTGCCTCTTTGTGGATGAAACACTAC1160.10066735513880813No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)