FastQCFastQC Report
Sat 31 Dec 2022
SRR5847674.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847674.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64652
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGGGTGAATCTCGTATGCCGTCT9821.5189011940852564No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAATCTCGTATGC9271.4338303532760008No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGA1940.3000680566726474No Hit
TTAGTAGGGAACAGGAGATAATGTTTGTGACATTGAGTTAGGCAAGTTTTTA1130.17478190929901627No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAA1000.15467425601682855No Hit
GGCAAAGGGCTCCTTGTTTTAGGCAAAGGCAGACCCACTATTCCCGATATTT990.15312751345666029No Hit
AGCTAGGGGAAGTGAGAATGCAGTGGAACAATATCCTCAAAGCATGAAATTA990.15312751345666029No Hit
ACAGTTGGGCGCCGGGGGAGGTTGTGGGCAACGGTGGAGGAGGAGAGACGGG940.14539380065581886No Hit
CACGATGGGTCAGGAAAAAAGGAAGATTGAACTAATAAAATTTTATTTCTTA930.14384705809565057No Hit
TGATATGGGCAGTAGGCTGTGTTCCAAGCAGGAATTATTTTCACTTACCAAA930.14384705809565057No Hit
GAATTGGGGGTGTCGACTCGTGGCTTATGAATAAAGGCAGGCAAAGTTTGCA920.14230031553548228No Hit
CACACAGGGACTGAATAGCATGTAAAACTTTCTCATGACTATATCCAAAAAT910.140753572975314No Hit
ACGCTTGGGGGCCTGGAGTTCAAATGATCTGTTCTTCTTTTAGTGCTGGTGG910.140753572975314No Hit
GCGTAAGGGCCGATCTCTTTAAGGGACCTGTGAGAGACCATCTAGCAGATTA910.140753572975314No Hit
AGCTGAGGGCAAGGTGGGAGTTAATAGCAAAAACTATTAAAAACCTAGCCAG910.140753572975314No Hit
GTCCATGGGGTGGAAAAAAAACATGGGAAGGAAAGGTAGGAAGAGAAAGCTT900.1392068304151457No Hit
GCCGTAGGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA900.1392068304151457No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAAACTCGTATGC900.1392068304151457No Hit
GTCAATGGGATTGTGGTATATCCATACCACAAAATACAACCCAGTAACAAAA880.13611334529480915No Hit
CACCAGGGGCAAGCTAACCAATATTTTAATTACAGATCATTAAGCAGCTTCG880.13611334529480915No Hit
AATGATGGGCAAATGGTCATTTAGTGGCATATGGGAGAGACGATAACCAGCA880.13611334529480915No Hit
AAATTAGGGAGCCACCTTGGTGACATAACAGAAACAAAATCATGTTGGTTAC870.13456660273464086No Hit
CAACAAAGGGTCGAGGGTTTGAGGCTTAAACAAGCCAACATATGAATATATG870.13456660273464086No Hit
ACTGCAGGGATTTGAAGAACTTGGAAGTAGTCATGGTTTAGCTGCATTGTCA870.13456660273464086No Hit
GTAGTTGGGCTCTCACTGTAGATTTGCTGTGCCTGTTCATGAATGGAATCAT860.13301986017447257No Hit
CAGAATGGGGCAAGACACACAGCAGGTTGTTCAGACACGGCTTCGCCCTAAT860.13301986017447257No Hit
GCTGCGGGCTTTCCTTAAAGTTCTTACAGTCCACGGGCTAATTTTCTTGTGT860.13301986017447257No Hit
CTAGCTGGGCGGGAATCTTACTGTTCAGGAGCTGTTCCTAGAACTAACTCCC850.13147311761430427No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGGGTGAATCCTGTCTCTTATAC850.13147311761430427No Hit
GTTGAGGGGAAGAACATCCTCACAGAGATCTACCGCATCGTGTCACAGAAAC830.1283796324939677No Hit
ATGCAAGGGGTCATTGCTCAATATTAATACATAGAGTAGGATTAGATTGAGG830.1283796324939677No Hit
GTGGTGGGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTC830.1283796324939677No Hit
GAGCTTGGGAAGGGACTTCTTTTTTAGAAATGCATTAAAAATAGTAAGCCTT820.12683288993379943No Hit
AGGCATGGGCACACTGCATTCGACTTGTTATTCTTTTGTGGCTTTGTTTTCT820.12683288993379943No Hit
CAAACTGGGACACCTGAAAAGTGAAACAAACTAAATTAAAATAAAATAAAAG820.12683288993379943No Hit
ATCAATGGGCGATCTCAGAGTGGGCTCCAGTGGTGAAGGGTGCAGCTCTGGT820.12683288993379943No Hit
TCCATTGGGATATGCCATCCGCGAGGCAGCCACCAGCAACCTGAAGAAGCTA820.12683288993379943No Hit
CTTTCGGGGACCTCCAGCAATGGACGCAGAAGAATTGAAGATCTATGAAATG820.12683288993379943No Hit
TCAGCCGGGATTGTAAAGGGATCAATTCAACAAGAAGAGCTAACTACCCTAA820.12683288993379943No Hit
TCTATTGGGCAAGCAGCGAGATTGTGCGAACCATGCTCCGAATGAGTGAGAT810.12528614737363114No Hit
CGACTGGGGATATGAAACCTGTCAATTCAAATGTCAGAACTTACGAGATTCT810.12528614737363114No Hit
TAAGCAGGGATGGAAGACTTCACACTGGCGAGAAGCCCTATGAATGTAAGGA810.12528614737363114No Hit
TGCTTAGGGTTGATATTTTAGTTATTAATTCATATTAACTTTGGCTGAAACT810.12528614737363114No Hit
ACAGTGGGGATTCATTCAAGAGGCAAGACTGTCTATCATAAAAACCTCCTAA800.12373940481346284No Hit
CGTGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC800.12373940481346284No Hit
ATACGAGGGACGTAACAGGAAGTGTGGGTACATCCAGTGACTAGAAAAAACT800.12373940481346284No Hit
CTTCGGGGGATGATATAGACTTTAAAAAATCTTAGTGCATTATTTTAATGTT800.12373940481346284No Hit
ATGAATGGGGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAA800.12373940481346284No Hit
TGTGAAGGGGAATATACATTCTTTTCAGCACCACACCACACCTATCCCAAAA800.12373940481346284No Hit
TCGTCAGGGTATCTTTGGAAACAGATTATTAGAGTAGTGGTTGACTGGGAAA800.12373940481346284No Hit
ACCGGCGGGGAGTGTCATGTTCTTCATTAATAAATATCTCATAGACAAGATC790.12219266225329457No Hit
TGGCAGGGGGAAGTTCTTCTTTATGTAAATGTGTTCTATGGGAGGGACAAGA790.12219266225329457No Hit
TTGATTGGGACATACAGTTTTATAATACTTCTCAAATGCAGATGGTCATGGA790.12219266225329457No Hit
GACATAGGGAGATGCCCCTGCACTTCCCTCCTCAAGTCATCTAAGAAAGAAT780.12064591969312627No Hit
GATGGGGGGGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTT780.12064591969312627No Hit
GGGGCGGGAGAATCGCTTGCATCCGGGAGGTGGAGGTTGCAGTGAGCCGAGA780.12064591969312627No Hit
AATTTAGGGCATCTATTTCTGAGTTGAATACTCAGCTCCCACGAGAAAGAAA780.12064591969312627No Hit
AGCAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC770.11909917713295798No Hit
TAGCAGGGGGGTCATCTAAAGCTGTCAAATAAGACATTCTGTGAAAGGTAAA770.11909917713295798No Hit
TCACAGGGGGCTAAGAGATTGGTCATATAATACAGACTTACAAGAGAGGCTA770.11909917713295798No Hit
AGTCAGGGGATGGTGTTGTATATTATAAACTGGTGACATTCTACTATTGAAT770.11909917713295798No Hit
AGACGTGGGGTATAAAAATGGACATTAAATCATGGCCTTGCATTAAAATATG770.11909917713295798No Hit
TGCGGCGGGGCATGGTGTTCGGATCTTTGGAATTAATAAGTATTGAAATGTT770.11909917713295798No Hit
TGGTCGGGGATTTGTGGTAATCTCATTTGTTGGTTGTACATCTGATCCTTTC760.11755243457278972No Hit
TATCTTGGGGAAAGGGTTCTAGGATCTCACACAAAAAGAATTCGAGGTGAAT760.11755243457278972No Hit
TACCGCGGGCGAGTATGAGCCACACAAAAAATTACAGTGAGAAAATGAGGAA760.11755243457278972No Hit
TCTGAAGGGGACTTTATGGAAAGAAGAAAAGCAACTTTCCTTTTTTGTACAG760.11755243457278972No Hit
GCAAGCGGGCTAGCAGAGTAAAAATACTGTATAAGTTCCGGGATTATTCAAG760.11755243457278972No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGGTGAATCTC750.11600569201262141No Hit
TAAGCTGGGACTGTCTTTCTGAAGGACGATTTCTTAATTCTTACCGAGAGCT750.11600569201262141No Hit
ACAAAAGGGGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCT750.11600569201262141No Hit
TTGTAGGGGATGTAGAAGGAGGTAATTTTCATGCCAGTTCACAGCAGTGGGG750.11600569201262141No Hit
CATTTCGGGAGGGGGTCAGGCACTGCATGCTCGTTCCAGCACCATCTGGGAC750.11600569201262141No Hit
GCTTGCGGGCAAAAAGAAACTATCATATGCGGTATACAAAGTATGACTAAGA740.11445894945245312No Hit
GTTAATGGGATGGAAGTAAGCAGTTTGACAGGTGGCTTATTTGTTTGATTGA740.11445894945245312No Hit
CGTGCAGGGAGGAGTTCCTAAGTGAGCTGCAGAGGAAGGAGGAAGAGATGAG740.11445894945245312No Hit
AGAAAAGGGAGAAGGGACCTTGGTGAAGGAGTGGTGGCTTTGAAACTTCTAT740.11445894945245312No Hit
CACTTAGGGTAACCAAGATGGACCTCAGCCTCCCAAGTAGCTGGGACTACAG740.11445894945245312No Hit
GCAGGTGGGCCTGACCACTCAATCTAAGAGAGCAACCTGTTATTCTTCATAT730.11291220689228486No Hit
TCGCCGGGGGGTTGTAAGTATATGGAGTAAATCCATCCATCTTCTGGAAGAA730.11291220689228486No Hit
TAGTTGGGGATAAAATTTCATGTATATCACATTGAAATGAAATTACAGAGAG730.11291220689228486No Hit
ACCCACCTCGGCCTCCCAAAATGCTGGGATTGCAGGCATGAGCCATTGCACC730.11291220689228486No Hit
TAGTGGGGGATCATGATGTCATAGCAATTTCCTGAGTACCCATTTTCAGATT730.11291220689228486No Hit
TTTACCGGGTATCACTGAATATCCAGTTGATTTATAAAACCCCAGCATCAAC720.11136546433211655No Hit
TCAGCGGGATGTGAGACCAAATTGCGTGTACTATAGCTGGGAGAACTTTTTG720.11136546433211655No Hit
ATTAAAGGGCAGAGCAGCAAAAATAGAAGGTACCATGTGTTCTCATTTTAAT720.11136546433211655No Hit
TCTGCTGGGTATAAATAGAGATATAATAAATTATCCCATTTTATAATATTCT720.11136546433211655No Hit
TTGGCCTGGGGCATCAGAGATGGTAATCTACAGCTCACCTCTGAAGGCAAAT720.11136546433211655No Hit
TATCCTGGGATATAAATAAAATTTGCTGTTAGCTTTAGACCTATATTCCTAA720.11136546433211655No Hit
TGACGTGGGCACATCTAAAAGCCCAGGAACTGATTTTTTTAAGAGGAAAACT720.11136546433211655No Hit
CGCAGCGGGCATTGATTCTTAGAAGAAAAGATTTGTTATTACAAATAAAATT720.11136546433211655No Hit
GAAATAGGGACATACATGTATACATATGATTTTGATGAACTAATCTATAAGT720.11136546433211655No Hit
TGAACTGGGCATATGCAATGCTATTGAGCCATTGTCCCCTCTTAGATTTTGT710.10981872177194826No Hit
TATTAGGGAACATATCGTCTGTTTTAATACGATTTTCTTTTTGAGTACTTGA710.10981872177194826No Hit
CACACTGGGATGGAGAAAGTTCATTAAAACATTCAGTGTATCTTGAGAAATT710.10981872177194826No Hit
CTGTAAGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTAT710.10981872177194826No Hit
AGTTCTGGGGACAGCATGGACTCTAGAGCGAGACCATCCTGGTTCAAATCCT710.10981872177194826No Hit
GTGCCAGGGATCCTGGTCTCCCACACTACTCATCCATCCATTCATTGTATTC710.10981872177194826No Hit
TGGTATCGGGATACCAAACTCATTTGCAGTTCTTAGGTTTGTTGGTTAAAAC700.10827197921178No Hit
CACTGGGGGATTTCAGTCTGATTAAAAGGAACGGAAGCATTTCCATCATAAG700.10827197921178No Hit
TGAAGTGGGCATGTATCCCAGAACTTAAAGTAAAATTTAAGAAATTAAAATT700.10827197921178No Hit
GTACTGGGGGATCTGCGTGTGGGATTTCAATATTTTCAATCCCTGTTTTACT700.10827197921178No Hit
GCTTTTGGGCTGGAAACAATAACATGTCTCTAAGCATTGTTTCTTGTGTTGT700.10827197921178No Hit
TTGCGAGGGCAGCCAAGACGGGGCCCCAACTCCTGGGCCCCCTAAGCCAGGC700.10827197921178No Hit
TCAGTGGGGTTGGGGTTAGATGTTGGGCAGGTTTTATCCTAGACTAATACAT700.10827197921178No Hit
TATAGAGGGCTAATACTTCAGCATCTTTTCTTAACCATAAACAAGAAGTGGA700.10827197921178No Hit
AACTAGGGGCAAGGCTCTTTCTTTGTACAGTCAGTCCAGAAGAGTCTCGGAA700.10827197921178No Hit
AACAGTGGGATGTGGGTCTATAATAATTGTATTTTCAAGAGGTTTTCACTAT700.10827197921178No Hit
TGCGCAGGGTTACCATGCTTAGTGAATCCGTTTCACATAGGTGTTTTGTTTT690.1067252366516117No Hit
CTGTGCGGGAAGTTACAGTGAGCTGTGATCACACCACTGCACTTTGGCCTGG690.1067252366516117No Hit
CTCAGTGGGGCAGCGGGGGTGGCGGAGGAGGCCTCATGGAGGAAATGAACAA690.1067252366516117No Hit
GGTACTGGGGATACAATCAGCAAAGTGTAAATGTGAGAGAAACTACAGGACA690.1067252366516117No Hit
TCTCCCGGGTAATGAGCTAAAATCTTATTTGGAAATATCCTTGTTAGAATTA690.1067252366516117No Hit
TCAGACGGGCAGGAAATGCAAATGCATGGGATTAGGAAATTCATTGGCTTTG690.1067252366516117No Hit
CTACAGGGACAGGCTCAAATTTACGTTTTTAAAGGAAGAATCTATGCAGCTG690.1067252366516117No Hit
GCTCAAGGGGGTGGTGACCAATGCATTGGTTACAGAAACTTTAAGTGAGTGC690.1067252366516117No Hit
TTAATAGGGAAACTGGAGGTTGACATCCCTTTGGTGAAATCCTATTTAGCAC690.1067252366516117No Hit
GGTTCAGGGATGATAAAGGAGGCATAACCAATCGGTGGGAAGAGGAAAAGAT690.1067252366516117No Hit
ACAACAGGAGGGATGGTTGCAGAAGAGTACAATGCCTTTTTCTACTCACTGG680.10517849409144343No Hit
TGGCAGGGGCACTGTAAAAATAAAGACAGTTATAGAACTGACATGTTCACAA680.10517849409144343No Hit
CTACTAGGGGGTTTCAGTCAGGGACATATTTTGTGCAATATTTCTGTGATTG680.10517849409144343No Hit
AGTGGCGGGGGTCACATAGAAAGTTAGAAATTTGTGATTAAAACCTCACGAT680.10517849409144343No Hit
AAACTAGGGACCATAAGGGGAAAGTTAATATTAGCTTTTGTTTGTAAACTAT680.10517849409144343No Hit
AAGCACGGGGTGGATCTCTAGCCCAGCTACAGCAGAATACATTTTACCAGCA680.10517849409144343No Hit
GGACAGGGAACGAAATCACAAAAGGGCTTTGTAAAATTATGATGTACCATTG680.10517849409144343No Hit
AACGCAGGGATGCCATAGTTAATATGTATGGCATTGAGGCTGCGCTGTGGGT680.10517849409144343No Hit
TCAAAAGGGGTGATAACATCTTTATACTTTGAAAAATGTTCCACTTACCCTT680.10517849409144343No Hit
GCACTGGGGATTTCGGAGTTATGGGAAGAAAGCTTTAAAGCTTTCAGCTTCT670.10363175153127514No Hit
ATTTAAGGGAAAGGATTCTGAGGAAGATACTAGTGGTCATGTTATACATTTA670.10363175153127514No Hit
CTCGCAGGGGTGAACCACTATCACGAAAAAAACTCTACCTCTCTATACTAAT670.10363175153127514No Hit
AAAATAGGGGGAAGGGGAAGGTGAGACATCTGAGTGAGCAGAGTGTGGCATC670.10363175153127514No Hit
CGTAATGGGTGGCTAAATCTTGGTATGTGGTTTTTAAACAGTCACTCCGTTT670.10363175153127514No Hit
TGAGTTGGGGGAGAGCATTAATTCCAAGGGCCAAATTTCACAGAGATTTCTG670.10363175153127514No Hit
CTCGACGGGACGTACATGTCAAAGTAATATAATGCCTTGTGGAATATTTAGC670.10363175153127514No Hit
AGCTAAGGGAAGCCTGACAAACCTTACAAAAATTGCATGATAGCATTTGGCT670.10363175153127514No Hit
TAGTGGGGGAGTGTACAAGTTAGTGCTTCTTTTTAATATGTTCACAAAGTTG660.10208500897110684No Hit
TTAGACGGGAGTTTCAAAAGCTTCTTTATAATTGTATTATTTTCTCTAATAT660.10208500897110684No Hit
GCCAGGGGCAGGTAAACTAAGTATATGTTTATTATTGGTGTATTTAATAAGA660.10208500897110684No Hit
TACATGGGGTATATGTATAGATCTTTCAAAATATATGACGGTATACCCGTAT660.10208500897110684No Hit
TGCGTGGGGCGGAGTCGGCATACCTGGCTTCCCCTCGAGGACCTCCCCTCAA660.10208500897110684No Hit
GCTTGAGGGACCCAGCTACAATCCACACCATTGATTCCATCTGTTGTTTCTA660.10208500897110684No Hit
AACGTGGGCTACCAGGCCACAGAAAGCGAGGCTGGTGATATGGACCTGAGTG660.10208500897110684No Hit
ATGCGAGGGATTGCAGCAGCCACTGGCTTTGTCAAAAAGGACCAGTTGGGCA660.10208500897110684No Hit
TGCACGGGGGGAGGAAAGGTGTGGCTATAAACTTTGTTACTGAAGAAGACAA650.10053826641093858No Hit
GAAGTTGGGAATGGAAAAATAGTTTTTTTTACTGGGTTCTCTTACCTTTTTT650.10053826641093858No Hit
ATACCTGGGATATCTGTAATAAATTAATTTTTTGCTCATAGTATTTGGTTAC650.10053826641093858No Hit
TTGATAGGGAGAGTGGCTCCACCCCAGTGATCTGGCCTCAGACAACTACTGG650.10053826641093858No Hit
ATGTCAGGGATGCCATTGCACTCCAGCCTGGGCAACAGGAGCAAAACTGCAT650.10053826641093858No Hit
CAAAAAGGGGTATACATGTATCAAAATATCACAAGTACCTCATAAATGTTTA650.10053826641093858No Hit
TCTCATGGGGGGGTGTACGCGGTGCTTCAGAGGATGGGAACTACAATTCCCA650.10053826641093858No Hit
CATGCTGGGAGAGATCTATTACTAAACACATTACAATCAAGCTGTCAAAAGC650.10053826641093858No Hit
AATAGCGGGATCGGAGTCAGCCAGGAGCTGTCACCAAGGAAACGCTACCTCT650.10053826641093858No Hit
GGTAACGGGCAGATTAAAATTAATTTCATGTGTATATGGTGGAAATTTGTGG650.10053826641093858No Hit
GCCTAAGGGGTGCAGTGTGACTGGCAGCAGAAAGGGGGCATCTACTGGTTTA650.10053826641093858No Hit
GACTGCGGGAATTCAGCAAAAGCAAAATGCTAAGACATATTTTAGTATATTT650.10053826641093858No Hit
GGCGAGCGGGTGTACACTTAGCCCTCAAGTTTACCATTGAGCCTAAACATGA650.10053826641093858No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)