FastQCFastQC Report
Sat 31 Dec 2022
SRR5847619.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847619.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88430
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGC4020.45459685627049645No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGCCGTCT3690.4172792038900825No Hit
TCCTAGGGGTTGTGGCTTGCTGTTACCAGCTGAGTGACCTTAAGGAAGTCAC1450.16397150288363677No Hit
TTGCTTGGGACAGCAGGAATCCTATAAATCACCACTATGGGCTGTGGCCATA1240.14022390591428247No Hit
GCGCTTGGGGGACACCAAAACATTTAAGGGGAAAGTAAAATGCTCCAAATGC1240.14022390591428247No Hit
CTGCACTGGGGGGAGACTCAAGCGATGTTTCTCCAAAGCAGCTTCTTGCACC1220.1379622300124392No Hit
CCGCTTGGGGAATAAATAAAAAGCATTTGTAAAGATTAAAAACACACATTAA1220.1379622300124392No Hit
CTAGAGGGATTTAAGTTATGTAAAAAATTTAATCATTATTTTGATGCTTTAA1180.1334388782087527No Hit
TTTCATGGGGGTAACTCAAATCACAAACATACAACAAATAGACACAAAAGTA1150.1300463643559878No Hit
GTCGCGGGGTGCTGAAAATACATATTTTAAAAATTAATTTAGAAGTTTCAGA1140.12891552640506615No Hit
AATCGAGGGCATTCTTTACCTCCTATGATTTAGCAATCGTGATGGAGTTGAC1140.12891552640506615No Hit
GGAGCGGGGACTTCCTGAAGGTGGACCTCCTCCTGGTCATGGGTACCTCCTT1130.12778468845414453No Hit
AATGCTGGGTCTAGCGGTGGGGGCGGCACGAGGGCTGGGCGGTGGGGTGCGG1110.12552301255230125No Hit
CTAGGTGGGGACGCTCTTCCGATCTCGTCAAGGGTGCACCTCCAAGAATCTG1100.12439217460137961No Hit
AGTCCTGGGACAGTTGAATTGTGTGACTTGAAGATTACCAATGATTTTGAGG1080.12213049869953636No Hit
AAGCCAGGGGTGCCCCGAGGTGAGGCGGCGGGGGCGGTGCAGGAGCTGGCGC1080.12213049869953636No Hit
ACGAAAGGGGATCTTGTGAATTAAGCCAAAAACTAAGTTTGATATAGTTGAA1080.12213049869953636No Hit
GCGTTGCGGGAGCTAAAGGAAGGATACTACCTTCTTACCTTCAGGTAGAAAA1070.12099966074861472No Hit
AGGATGGGAAAAGTCATGCGAGGATGACATACTAAAAAAATAAGATTTGCCA1070.12099966074861472No Hit
GTAGAGGGGATATTTTTACAACTAGATGAGGTATTCCTAGGACTCATTCCTG1060.1198688227976931No Hit
ACATACGGGGAGAGGCGACCTAAACATGGAGACAGCGGGCGCTGCAACTGGG1050.11873798484677146No Hit
CTCATCGGGAGAAATAAAAAGTATCTAAATAGAAAAGGAGGAAGGGAAATTG1030.11647630894492818No Hit
GCACAAGGGCACCACTGCACTCCAGTCTGCTGGACAACAGAGCAAGACTCAG1030.11647630894492818No Hit
TATGGCAGGGCATGTCCTGAAGGTCACCTTTGCCTTTGAAAAAGGTTTGATG1020.11534547099400656No Hit
ACAATGGGGATTCCGGGTGGTTGGCAAACTCATCGTGTCTGTCCTGAGAGGC1020.11534547099400656No Hit
ATATCAGGGCTTAAGTCAGACTTAAAGATTTGAGAAATTATTTGTGTCATTT1000.11308379509216329No Hit
TGTAGTGGGAGCTTGTTTGTTGCTGAAATGCTACTTTTTAAAATTTAGATGT1000.11308379509216329No Hit
CTTGCCGGGTGCTTTTAGAAATTAACTGTGTGGCACACTTATTTTTTGTGCA1000.11308379509216329No Hit
ACGCCCGGCCCTTCAGCTTGATAGAAGTACAAAGAGAAGGCTAGGGAAATAA990.11195295714124168No Hit
CAAAAACAAAAAAGATGCAAAAAGCAATTATAAAATGATGTTTGTTAAATCC990.11195295714124168No Hit
ACTGGTGGGACAGGAGGCCCTTGGGAGCCCTCAGATAACTTTCTCATTCTTC990.11195295714124168No Hit
AGCACAGGGGAGCGCACCCAGGCGCCCGACGCCGCCCGGGTCTGCGCCGCTG990.11195295714124168No Hit
TTGTACGGGAGCAATGTTGAGTGTGACTAAACATCCAAACATTATATCCTTT980.11082211919032003No Hit
GCACACGGGCAAATCGGCCTGGATGTTTGAGGTGTTTCATAGGGGCTTTTCG980.11082211919032003No Hit
CGAGGAGGGGATCTTGGATGGGGGATCCGCCGGACGCCGGAAGTGGTTCTCC980.11082211919032003No Hit
ACGTACGGGGCTTCCTATTTCTGGTGTTTATCATTTTGGTTATTACCTGTTC980.11082211919032003No Hit
GAGCTTGGGAGGGTGGGTCTCATCTTCAAGTCTGAAGACAGACTCCCTCCAG980.11082211919032003No Hit
CCGACTGGGAGGTGTGCACCGCCACACCCAGCTAATTTTTGTGTTTTTTGTA980.11082211919032003No Hit
TACAAGGGGAAACAGAAAGGGTTACAAAAGAGATGAATGAATTTATCCATAA970.10969128123939839No Hit
TTCAGCGGGCATCAACAGACACTTCTCAAAAGAACAAGTGGCCAATAAACAG970.10969128123939839No Hit
GGTGCTGGTGGCATCAATGGTCATGCTGACAGCCCTGCTCCTGTGAACGGGA970.10969128123939839No Hit
ATTCGAGGATCTCAGATAGAGAAATACAAACCACCCTTTTACTCATTCACTG960.10856044328847675No Hit
AAACACGGGAGACACCTGGATACAGGAAGCTCAGAGATTCCCCAAGTAGATT960.10856044328847675No Hit
GTATCAGGGACTTGATATCAGGCTCATCATATGCCCGAAGCTCAGTGGTTAC960.10856044328847675No Hit
CACAACGGGAATGGAAACTCATTAAATATGTTTATATCCATGTTTGAATAAT950.10742960533755513No Hit
TGTAGCGGGAAAGTGAACATCAAAAACTACATGTCTCAATACTTCCAGAAAA950.10742960533755513No Hit
TACCGACGGGCTGATTCAACACTCTGCCACGATGCCCTGAGTAAATTGTCAA950.10742960533755513No Hit
GACTGAGGGGGACATGTTAACCTTAAACGGGCTCAGATGGCATGTTCAGATA940.10629876738663349No Hit
TATACTGGGCGCTGAAAGCTGTGCAGCGCATTGCTGAGTCTCACCTGCAGTC940.10629876738663349No Hit
AAATATGGGAAAGACAAGAAATTATGTACATGTACTAATGTTTTTCCCACAA940.10629876738663349No Hit
CACAGGGTGTTCTGAGGAGTGACCTCTGTAGCCTGGTCGTTTCTCCACAGGC940.10629876738663349No Hit
AAAAAGGGGAGGGTGGTGTGTCCTGGGAGGGCATGGAATCTCTGTGCCACCT940.10629876738663349No Hit
AATTCCCTGGATTATTTTTGTTGTGTTATTAAATAACTTAGAATTTTTTTTA940.10629876738663349No Hit
GGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTA930.10516792943571186No Hit
ACAGTAGGGGGTCATAAGCCTGGTAATATCCCAGAAAGGGATTTCTCCATCA930.10516792943571186No Hit
TTCAATGGGCTATGGTAATGGCTAATATACCATGATGTTTATAGGAGCAAAA930.10516792943571186No Hit
CTGTCAGGGGACTAAGTTCATTAAGCTGCTCGTGTTTTTATGCCTTGACAGT920.10403709148479023No Hit
CTTAGCGGGAGGATCTGTGGCCAAATCCACTGCAATACTACCTGTTGCGTGA920.10403709148479023No Hit
ACTGCCGGGGTGTGGCCCTTGAGCCATGTGGTGTTAGCGTGACATTGGAGAG920.10403709148479023No Hit
TATACAGGGGCGCGGAAGCGCCGCGGTAGGGTGGGAACCCAAGCGGGAGAGC910.1029062535338686No Hit
AACATAGGGATGTCTTCTTTAGACAATAAGTTCTTTAATGTAAAGAACTTCT910.1029062535338686No Hit
AAGACGGGATGACTGTATTTATGTTTTTGCATAAAATAGCTGAATTTTCAGT910.1029062535338686No Hit
GCGCAGGGGTATTTCAGGCTTTCAATTTATTCATGCTTTCATCTGTTTAAAC910.1029062535338686No Hit
ACACAGGGATAGCTCAGTTTGACTTCTCAAACTTTTCAAGGGTTGGGGCAGC910.1029062535338686No Hit
TAAGTAGGGCAGACAAACAAATGCCGAGTGAGTCACCACTACAAAGCCAGCA910.1029062535338686No Hit
ATCTCCCATTTCCAGTAACACCCCATTTGTTTTATTTATGAGGTATTTATCC910.1029062535338686No Hit
GAGTTTGGGCACTGCTTCCCTGAGATTTGATGGAGCCCTGAATGTTGACCTG910.1029062535338686No Hit
TGTTTGGGGGGAAAATATCTGGAACAAATTTGTCAAGATAGTTAACAGAATT900.10177541558294696No Hit
GATTCGGGGCAACCAGAAAAGGAAAAAAGAGAGTGGAAGACAAAAACAAAGA900.10177541558294696No Hit
TAAAAAGGGATATATAGCCCGTCCAACATTTCGCTAATAATGTGACGTATCT900.10177541558294696No Hit
GGTCACGGGAACAGGAAGCCGAGCTCATTTTTCCTGGAACATAGCTCTAACA900.10177541558294696No Hit
AATGCAGGGCTAACACTAATAAAAAGAAAGCGGAAGAATAGATGAATCCACT900.10177541558294696No Hit
TACTACGGGGTTTCATGAACAATATGATACAATAGATTGTCCTAATAATTAC900.10177541558294696No Hit
CACAGAGGGATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTG900.10177541558294696No Hit
TGGCCCGGGTGTGTCAAGGTTGCAACAGAATTTTCACAATTTTTTTGTTGTT890.10064457763202532No Hit
CTTTCGGGGGTAAATTAAAACCAAAGACAAGATTAGCGGCTTCTACTATTTG890.10064457763202532No Hit
CTTCACGGGTGGCTTTGATTTGGTGACATGTAGCAGTTCTGGAAGAATTAAC890.10064457763202532No Hit
CTGTTTGGGGACAGTATGTAGTTGAGTGGAGGCTGGGAGTTTTAAGCATAAA890.10064457763202532No Hit
TAACTGGGAAGCGCGGTTACTATGGCCAGTCTGCCCGATACCGGGACACAGA890.10064457763202532No Hit
GCTTTCGGGGGGAGCTGCTGGTACTCCCGATTGGAGACGTAGAACCGTTACT890.10064457763202532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)