FastQCFastQC Report
Sat 31 Dec 2022
SRR5847618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88230
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGC4360.4941629831123201No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGCCGTCT3640.4125580868185425No Hit
TCCTAGGGGTTGTGGCTTGCTGTTACCAGCTGAGTGACCTTAAGGAAGTCAC1340.15187577921341947No Hit
CTGCACTGGGGGGAGACTCAAGCGATGTTTCTCCAAAGCAGCTTCTTGCACC1300.14734217386376516No Hit
TTTCATGGGGGTAACTCAAATCACAAACATACAACAAATAGACACAAAAGTA1210.13714156182704296No Hit
GGAGCGGGGACTTCCTGAAGGTGGACCTCCTCCTGGTCATGGGTACCTCCTT1210.13714156182704296No Hit
GCGTTGCGGGAGCTAAAGGAAGGATACTACCTTCTTACCTTCAGGTAGAAAA1190.1348747591522158No Hit
ATTCGAGGATCTCAGATAGAGAAATACAAACCACCCTTTTACTCATTCACTG1160.13147455513997508No Hit
ACTGGTGGGACAGGAGGCCCTTGGGAGCCCTCAGATAACTTTCTCATTCTTC1130.12807435112773433No Hit
GTCGCGGGGTGCTGAAAATACATATTTTAAAAATTAATTTAGAAGTTTCAGA1120.12694094979032075No Hit
GCACAAGGGCACCACTGCACTCCAGTCTGCTGGACAACAGAGCAAGACTCAG1120.12694094979032075No Hit
TTGGGCGGGGTTCCAAAGTGAGTATCTCACTTACCTGACCCCTCACCCAAGT1110.12580754845290718No Hit
GATTCGGGGCAACCAGAAAAGGAAAAAAGAGAGTGGAAGACAAAAACAAAGA1090.12354074577808002No Hit
CCGCTTGGGGAATAAATAAAAAGCATTTGTAAAGATTAAAAACACACATTAA1090.12354074577808002No Hit
CATGGAGGGGGGGATAACGGCGGCGAGCGGACGGCTGCATTTACGGGGTCTC1080.12240734444066645No Hit
TATACAGGGGCGCGGAAGCGCCGCGGTAGGGTGGGAACCCAAGCGGGAGAGC1080.12240734444066645No Hit
TGTAGCGGGAAAGTGAACATCAAAAACTACATGTCTCAATACTTCCAGAAAA1080.12240734444066645No Hit
AATCGAGGGCATTCTTTACCTCCTATGATTTAGCAATCGTGATGGAGTTGAC1070.12127394310325287No Hit
TTGCTTGGGACAGCAGGAATCCTATAAATCACCACTATGGGCTGTGGCCATA1050.11900714042842571No Hit
TGTAGTGGGAGCTTGTTTGTTGCTGAAATGCTACTTTTTAAAATTTAGATGT1050.11900714042842571No Hit
TACAAGGGGAAACAGAAAGGGTTACAAAAGAGATGAATGAATTTATCCATAA1040.11787373909101213No Hit
CCTGGGGGGAAACTAACCAGTTTTTTAAACTTTAATTCTTAAATTTATGGTT1040.11787373909101213No Hit
GGTGCTGGTGGCATCAATGGTCATGCTGACAGCCCTGCTCCTGTGAACGGGA1040.11787373909101213No Hit
CGTACAGGGAAGGACATCCTGCTGATGCTGAGTGACATGGACATCAACGCCA1030.11674033775359856No Hit
TGGCCCGGGTGTGTCAAGGTTGCAACAGAATTTTCACAATTTTTTTGTTGTT1030.11674033775359856No Hit
AGCGGTGGGAAACTTATGAATTGCAGCAAAAGCAATACTAAGAGAGAAGTTT1030.11674033775359856No Hit
CGGGGGATCTGGATTTTAAAAGTTTTGCATAAACGATTAGCTTTCAAGCTTG1030.11674033775359856No Hit
GAGTTTGGGCACTGCTTCCCTGAGATTTGATGGAGCCCTGAATGTTGACCTG1030.11674033775359856No Hit
AAATAGGGTTCTTAGCCTTCTGTAAAACATGCTAAATATTCTTAGGCGTTCC1020.11560693641618498No Hit
AACAAAGGGGGAGTTCAAGACCAGACTAGACCACATGGCGAAACCCCGTCTC1020.11560693641618498No Hit
AAATATGGGAAAGACAAGAAATTATGTACATGTACTAATGTTTTTCCCACAA1020.11560693641618498No Hit
AGGATGGGAAAAGTCATGCGAGGATGACATACTAAAAAAATAAGATTTGCCA1020.11560693641618498No Hit
AATATAGGGACCACGATTTTCTAAAGTGCATATTAGAATTTTTGTTTTTGTA1010.1144735350787714No Hit
TTAGTGGTGGGGTAAGTTACACATTTGAAAAGTCGGTTTTTTCTTCAGGCTA1010.1144735350787714No Hit
TACCTAGGGGCTTTTCAACATCATACTAGAAGTTCTAGCTCATGCAATATGA1000.11334013374135782No Hit
GCACACGGGGAAATAAATTAGATCAATCATTGTCCTTAGAAAGCTTACAGTA1000.11334013374135782No Hit
GCTTTCGGGGGGAGCTGCTGGTACTCCCGATTGGAGACGTAGAACCGTTACT990.11220673240394424No Hit
TTTCGGGGGCTGGAGTGCAGTGGTGTGATCTCAGTTCACTGCAACCTCTGCC980.11107333106653067No Hit
TATGGCAGGGCATGTCCTGAAGGTCACCTTTGCCTTTGAAAAAGGTTTGATG970.10993992972911709No Hit
CTGTTAGGGGTTAAAAATGCTGTCAAATCCTTAATAGCTACAGGAGCTACTG970.10993992972911709No Hit
TTCAGCGGGCATCAACAGACACTTCTCAAAAGAACAAGTGGCCAATAAACAG970.10993992972911709No Hit
TACCGGGGATGTAAACCTTTCCAGAAAATTTAAGAGAAATCATTCTTAACTC970.10993992972911709No Hit
AATGCAGGGCTAACACTAATAAAAAGAAAGCGGAAGAATAGATGAATCCACT970.10993992972911709No Hit
GCACACGGGCAAATCGGCCTGGATGTTTGAGGTGTTTCATAGGGGCTTTTCG960.10880652839170352No Hit
TAGCCGGGGAAGTAGATTTTTTTTAACCATGGACAATTGGTAATTTTAAACA960.10880652839170352No Hit
GTATATGGGAACTTACTGGTTTTTCAGTTTTAGGCATAGTTTATCTGCATTT960.10880652839170352No Hit
ACGAAAGGGGATCTTGTGAATTAAGCCAAAAACTAAGTTTGATATAGTTGAA960.10880652839170352No Hit
TAAACTGGGCTCTAAGAGGGGCTGATAAGCAACTTCAGCAAAGTCTCAGGAT950.10767312705428993No Hit
AAATGCGGGACTCTGCAGATAGTGAAAAAGATGGAGGGCCCCAATTTGCAGA950.10767312705428993No Hit
AGCCATGGGCATTTGCAGGTTATTGAAAACTAATCAATCTTCTATGATTAAC950.10767312705428993No Hit
GAGTGTGGGGGAGCTGGGAGACAAGCCCGCGCCCAACACCTTCTATGTAGGC950.10767312705428993No Hit
TCTCACGGGAGAAATTGAAGATGCTTCACCCACCAAGTGTCCAATAACAACC950.10767312705428993No Hit
CCACGGGAAGATTTCCAGTACTATGTTGAGTAGGAGTGGTGAGAGAGGGCAT950.10767312705428993No Hit
CGAGGAGGGGATCTTGGATGGGGGATCCGCCGGACGCCGGAAGTGGTTCTCC940.10653972571687635No Hit
AGATGCGGGGCCCAGACAGCTGGGGTTCACTCAGAGAGGACCCAAGTCCCAG940.10653972571687635No Hit
GTCCATGGGATAACCATAAGACAAGGAACCTAAAATCCTCATGATCTCATTT940.10653972571687635No Hit
CTTGCCGGGTGCTTTTAGAAATTAACTGTGTGGCACACTTATTTTTTGTGCA940.10653972571687635No Hit
GATAAAGGGGTAACCACCATTCTACTCTCTGCTTCTATGAGTTCAAGCTTTT930.10540632437946278No Hit
ACGCCCGGCCCTTCAGCTTGATAGAAGTACAAAGAGAAGGCTAGGGAAATAA930.10540632437946278No Hit
CTGTTTGGGGACAGTATGTAGTTGAGTGGAGGCTGGGAGTTTTAAGCATAAA930.10540632437946278No Hit
TAAGTAGGGCAGACAAACAAATGCCGAGTGAGTCACCACTACAAAGCCAGCA930.10540632437946278No Hit
CTACGCGGGCTTGAAGAAATAGCATCTATAGGAAATGCGGCAGCAGGAGGAC930.10540632437946278No Hit
GAGCAGGGGAGGGAAAGAAACCAGGCATCTGGTCAAACCAGCAGATCAAAAA930.10540632437946278No Hit
TAATGTGGGCGTCATTTAAATTAAAAGTGAAAAGACACTAGAGAAAAATCAA920.1042729230420492No Hit
ATTATGGGGCAACTATGATAACATAGTGTTAGAATTTTCTTGGTGAACTTAC920.1042729230420492No Hit
CAAAAACAAAAAAGATGCAAAAAGCAATTATAAAATGATGTTTGTTAAATCC920.1042729230420492No Hit
TATATAGGGTATGCGTAATGCTCAGCTTTTTGTAAAGAAATATTTAATTGTA920.1042729230420492No Hit
ACACAGGGATAGCTCAGTTTGACTTCTCAAACTTTTCAAGGGTTGGGGCAGC920.1042729230420492No Hit
AAGACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.1042729230420492No Hit
GCTGCTGGGAGAGGAATGTGACACATCAGCCTAGAAAATCACTTGAAAAGAT920.1042729230420492No Hit
CACAGGGTGTTCTGAGGAGTGACCTCTGTAGCCTGGTCGTTTCTCCACAGGC920.1042729230420492No Hit
TTGTACGGGAGCAATGTTGAGTGTGACTAAACATCCAAACATTATATCCTTT910.10313952170463563No Hit
GGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTA910.10313952170463563No Hit
TCCGGGGTATGCAGGGAATGTGTTCGGTGATTTCAGAAAGCTAGAAAGTGTA910.10313952170463563No Hit
TATGCAGGGGATGAATAGGGAAATGCCAAACTGCAGCAGCGCCAGCTCATAA910.10313952170463563No Hit
TCATTAGGGGAATAAAGCTACACTTCATCAAATAAGGAAACGGGTTTGCATA910.10313952170463563No Hit
TTCCTTGTGGGAGGCGTGGTGCGCGGTAGGAGCTGGGCGCGCACGGCTACCG910.10313952170463563No Hit
CAATGAGGGGAAGTTCATACTGCCAACATACTTTTCACTGAAACAATTTTTA910.10313952170463563No Hit
AGCACAGGGGAGCGCACCCAGGCGCCCGACGCCGCCCGGGTCTGCGCCGCTG910.10313952170463563No Hit
ACAATGGGGATTCCGGGTGGTTGGCAAACTCATCGTGTCTGTCCTGAGAGGC900.10200612036722204No Hit
CTCATCGGGAGAAATAAAAAGTATCTAAATAGAAAAGGAGGAAGGGAAATTG900.10200612036722204No Hit
GAGAATGGGATCCCATAGGTTTTGGTAAGTTGTGTTTCCATTTTCTTTTATT900.10200612036722204No Hit
TAAACAGGGGACACCTTTCAAAGAATAAATGAAAAATAGAGAGACATACCTG900.10200612036722204No Hit
CTAGGACAGGGCAAGCCCCTTGTTCACAAATTAAACAATTAAAATAAATTTT890.10087271902980846No Hit
GCATCAGGGCTTGAGGTTGGGGCTTAGGCCCCTACCAGCCCAGCCCCCAGTG890.10087271902980846No Hit
TTATGGGGGATGTGAATATGATGGAATTCATAGGAATTTGTTCTGAATGAGA890.10087271902980846No Hit
ATCTCCCATTTCCAGTAACACCCCATTTGTTTTATTTATGAGGTATTTATCC890.10087271902980846No Hit
ACATCTGGGGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCACGGCC890.10087271902980846No Hit
TCACTTGGGATCCAAGCAAAAACAAATCTGCTCTTAATGTGGAAACTGTGGC890.10087271902980846No Hit
AATTCCCTGGATTATTTTTGTTGTGTTATTAAATAACTTAGAATTTTTTTTA890.10087271902980846No Hit

[OK]Adapter Content

Adapter graph