FastQCFastQC Report
Sat 31 Dec 2022
SRR5847617.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847617.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87832
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGC3830.43605975043264417No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGCCGTCT3610.4110119318699335No Hit
CTGCACTGGGGGGAGACTCAAGCGATGTTTCTCCAAAGCAGCTTCTTGCACC1360.1548410602058475No Hit
GCGTTGCGGGAGCTAAAGGAAGGATACTACCTTCTTACCTTCAGGTAGAAAA1260.14345568813188814No Hit
ATTCGAGGATCTCAGATAGAGAAATACAAACCACCCTTTTACTCATTCACTG1210.13776300209490847No Hit
GTGCGAGGGTTATCCTTGAAATGCACTAAGCCTCATGTTAACTGCTCAAATA1210.13776300209490847No Hit
TATGGCAGGGCATGTCCTGAAGGTCACCTTTGCCTTTGAAAAAGGTTTGATG1190.13548592768011658No Hit
TCCTAGGGGTTGTGGCTTGCTGTTACCAGCTGAGTGACCTTAAGGAAGTCAC1190.13548592768011658No Hit
CACTTAGGGGTGATCTAATTTATTTTCTTTTGGTTTCTTCTTTACATTTACT1170.13320885326532472No Hit
GGAGCGGGGACTTCCTGAAGGTGGACCTCCTCCTGGTCATGGGTACCTCCTT1170.13320885326532472No Hit
CCGCTTGGGGAATAAATAAAAAGCATTTGTAAAGATTAAAAACACACATTAA1170.13320885326532472No Hit
TTTCATGGGGGTAACTCAAATCACAAACATACAACAAATAGACACAAAAGTA1140.12979324164313688No Hit
TTCAGCGGGCATCAACAGACACTTCTCAAAAGAACAAGTGGCCAATAAACAG1130.12865470443574095No Hit
TTGCTTGGGACAGCAGGAATCCTATAAATCACCACTATGGGCTGTGGCCATA1110.1263776300209491No Hit
GTATATGGGAACTTACTGGTTTTTCAGTTTTAGGCATAGTTTATCTGCATTT1100.12523909281355314No Hit
TAAAAAGGGATATATAGCCCGTCCAACATTTCGCTAATAATGTGACGTATCT1100.12523909281355314No Hit
CGAGGAGGGGATCTTGGATGGGGGATCCGCCGGACGCCGGAAGTGGTTCTCC1080.12296201839876128No Hit
GATTCGGGGCAACCAGAAAAGGAAAAAAGAGAGTGGAAGACAAAAACAAAGA1080.12296201839876128No Hit
AGGATGGGAAAAGTCATGCGAGGATGACATACTAAAAAAATAAGATTTGCCA1080.12296201839876128No Hit
CAAAAACAAAAAAGATGCAAAAAGCAATTATAAAATGATGTTTGTTAAATCC1080.12296201839876128No Hit
ACGCCCGGCCCTTCAGCTTGATAGAAGTACAAAGAGAAGGCTAGGGAAATAA1070.12182348119136534No Hit
AGTCCTGGGACAGTTGAATTGTGTGACTTGAAGATTACCAATGATTTTGAGG1060.12068494398396941No Hit
TCAAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.11954640677657347No Hit
CTAGGTGGGGACGCTCTTCCGATCTCGTCAAGGGTGCACCTCCAAGAATCTG1050.11954640677657347No Hit
GTAGAGGGGATATTTTTACAACTAGATGAGGTATTCCTAGGACTCATTCCTG1040.11840786956917751No Hit
CTGTTAGGGGTTAAAAATGCTGTCAAATCCTTAATAGCTACAGGAGCTACTG1030.11726933236178158No Hit
AGGTAGGGGATCCAGGAATAGAGCATGTATGTATTAGTATACGAGAGAGGTA1030.11726933236178158No Hit
CAGGTCAGGTACCTTGGTGATCAGCTACTAGTTCTTCCAGCCCTCATTGAGG1020.11613079515438564No Hit
ATCTCCCATTTCCAGTAACACCCCATTTGTTTTATTTATGAGGTATTTATCC1020.11613079515438564No Hit
TAAGTAGGGCAGACAAACAAATGCCGAGTGAGTCACCACTACAAAGCCAGCA1010.11499225794698971No Hit
ACGAAAGGGGATCTTGTGAATTAAGCCAAAAACTAAGTTTGATATAGTTGAA1010.11499225794698971No Hit
AATATAGGGACCACGATTTTCTAAAGTGCATATTAGAATTTTTGTTTTTGTA1010.11499225794698971No Hit
TTTGAAGGGGATGTGTTGTTGAGACATTCTTGTTTTTCCCTACAACTTATCT1010.11499225794698971No Hit
AAACACGGGAGACACCTGGATACAGGAAGCTCAGAGATTCCCCAAGTAGATT990.11271518353219784No Hit
TGAGATGGGAATACAGTGTGTGTTATTACGAGGAGCCAGCAGTTGAGCCTAA980.1115766463248019No Hit
ACTTACGGGGGGCAGAGGCAGGCCACATTCCCACTGGGAAGCATGGCTGTTA980.1115766463248019No Hit
GACCCGGGGTGTCTCATTTTAAGAAATTGCCACAGCAACCCCAACCTTCAGC980.1115766463248019No Hit
ACATACGGGGAGAGGCGACCTAAACATGGAGACAGCGGGCGCTGCAACTGGG980.1115766463248019No Hit
AACGTTGGGCAAAAGAAGACATACATGTGGCTGATAAGCACATGAAAATTGC970.11043810911740597No Hit
TACAAGGGGAAACAGAAAGGGTTACAAAAGAGATGAATGAATTTATCCATAA970.11043810911740597No Hit
ACTGGTGGGACAGGAGGCCCTTGGGAGCCCTCAGATAACTTTCTCATTCTTC970.11043810911740597No Hit
AAATGCGGGACTCTGCAGATAGTGAAAAAGATGGAGGGCCCCAATTTGCAGA970.11043810911740597No Hit
TCCGGGGTATGCAGGGAATGTGTTCGGTGATTTCAGAAAGCTAGAAAGTGTA970.11043810911740597No Hit
TCGTTGGGTATTCTAACTTGTAAATTATATAGCAGCTGGGATGATGTATGTG970.11043810911740597No Hit
GTCGCGGGGTGCTGAAAATACATATTTTAAAAATTAATTTAGAAGTTTCAGA960.10929957191001002No Hit
CTCATCGGGAGAAATAAAAAGTATCTAAATAGAAAAGGAGGAAGGGAAATTG960.10929957191001002No Hit
TGTTCCGGGTGCCAAGAAAAGTTTCTGATGTTTATGTGTGATGTACAGTGAT960.10929957191001002No Hit
GATTCAGGGCAGCTCACTGCAGCCTCCGCCTCTCAGGTTCAAGAAATTCTTC960.10929957191001002No Hit
CTAGAGGGATTTAAGTTATGTAAAAAATTTAATCATTATTTTGATGCTTTAA950.1081610347026141No Hit
AAGCCAGGGGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTT950.1081610347026141No Hit
AGCGGTGGGAAACTTATGAATTGCAGCAAAAGCAATACTAAGAGAGAAGTTT950.1081610347026141No Hit
GACAATGGGGATTTAAAAATACCGAGGTTTATTTCTCATTTGCGCTGTATCT950.1081610347026141No Hit
GGTCACGGGAACAGGAAGCCGAGCTCATTTTTCCTGGAACATAGCTCTAACA950.1081610347026141No Hit
CGGGGGATCTGGATTTTAAAAGTTTTGCATAAACGATTAGCTTTCAAGCTTG940.10702249749521815No Hit
GAGCTTGGGAGGGTGGGTCTCATCTTCAAGTCTGAAGACAGACTCCCTCCAG940.10702249749521815No Hit
GATAAAGGGGTAACCACCATTCTACTCTCTGCTTCTATGAGTTCAAGCTTTT940.10702249749521815No Hit
CGATAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC930.1058839602878222No Hit
TGGCCCGGGTGTGTCAAGGTTGCAACAGAATTTTCACAATTTTTTTGTTGTT930.1058839602878222No Hit
TGTTTGGGGGGAAAATATCTGGAACAAATTTGTCAAGATAGTTAACAGAATT930.1058839602878222No Hit
CGTACAGGGAAGGACATCCTGCTGATGCTGAGTGACATGGACATCAACGCCA920.10474542308042625No Hit
AGATTGGGGAGGCCAAATGTGTAGTTGAGAATTTTAAAGCAAAATTTAAATA920.10474542308042625No Hit
TAATGTGGGCGTCATTTAAATTAAAAGTGAAAAGACACTAGAGAAAAATCAA920.10474542308042625No Hit
ACAAATGGGTGTAATACAAGTTGGTTTAGAACCAAAGTGCTTTCTAACTTTC920.10474542308042625No Hit
AGTCTGGGGCGTTTCCGTGGAATCAGGCCGGCTGGTGAGGGTACAGAATGGA920.10474542308042625No Hit
ACAGTAGGGGGTCATAAGCCTGGTAATATCCCAGAAAGGGATTTCTCCATCA920.10474542308042625No Hit
AGATGCGGGGCCCAGACAGCTGGGGTTCACTCAGAGAGGACCCAAGTCCCAG920.10474542308042625No Hit
GCGCTTGGGGGACACCAAAACATTTAAGGGGAAAGTAAAATGCTCCAAATGC910.10360688587303032No Hit
AGCACAGGGGAGCGCACCCAGGCGCCCGACGCCGCCCGGGTCTGCGCCGCTG910.10360688587303032No Hit
AAGACGGGATGACTGTATTTATGTTTTTGCATAAAATAGCTGAATTTTCAGT910.10360688587303032No Hit
TAAACAGGGGACACCTTTCAAAGAATAAATGAAAAATAGAGAGACATACCTG910.10360688587303032No Hit
AATCGAGGGCATTCTTTACCTCCTATGATTTAGCAATCGTGATGGAGTTGAC910.10360688587303032No Hit
CTGTTTGGGGACAGTATGTAGTTGAGTGGAGGCTGGGAGTTTTAAGCATAAA910.10360688587303032No Hit
GACTGAGGGGGACATGTTAACCTTAAACGGGCTCAGATGGCATGTTCAGATA900.1024683486656344No Hit
TGTAGCGGGAAAGTGAACATCAAAAACTACATGTCTCAATACTTCCAGAAAA900.1024683486656344No Hit
TCATCGGGGATTAATTATCATATGCTTTTTTTAATGAAGTGCTTGATCACTT900.1024683486656344No Hit
AATGCAGGGCTAACACTAATAAAAAGAAAGCGGAAGAATAGATGAATCCACT900.1024683486656344No Hit
ATAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.10132981145823845No Hit
AATGCTGGGTCTAGCGGTGGGGGCGGCACGAGGGCTGGGCGGTGGGGTGCGG890.10132981145823845No Hit
ATGTACGGGGAATCTTCAGATTGATCATCATTGCTTGCTGAAAAGTCGAAAT890.10132981145823845No Hit
CACAGGGTGTTCTGAGGAGTGACCTCTGTAGCCTGGTCGTTTCTCCACAGGC890.10132981145823845No Hit
CTGAGATAGGGATAAATATCTAATTGGAGCTCTGGAAAGAAAAAATGGGACA890.10132981145823845No Hit
TGGCCTACCAAAGTGCTGGGATTGCAGGCGTGAACCACTGTGCCGGGCCAGA880.10019127425084251No Hit
ACCTAGGGGACTAATCTCTGGCTGAGGGATGACTTACCTGTTCAGTACTCTA880.10019127425084251No Hit
CACAACGGGAATGGAAACTCATTAAATATGTTTATATCCATGTTTGAATAAT880.10019127425084251No Hit
CTTGCCGGGTGCTTTTAGAAATTAACTGTGTGGCACACTTATTTTTTGTGCA880.10019127425084251No Hit
GGTCTAGGGACGGGATTTGACTTTAAATCCTGGGAATATCTTTGAAATGAAT880.10019127425084251No Hit
TACCTAGGGGGCGTCTTGTTCTTGCCTGGTGTCGGTGGTTAGTTTCTGCGAC880.10019127425084251No Hit
TATACTGGGCGCTGAAAGCTGTGCAGCGCATTGCTGAGTCTCACCTGCAGTC880.10019127425084251No Hit
CGAACTGGGTTCATGTCCAGTGAGCAGTGTTGCGTTTTTCCTTGTAGCATTT880.10019127425084251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)