FastQCFastQC Report
Sat 31 Dec 2022
SRR5847616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80220
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGC5140.6407379705809025No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGCCGTCT4590.5721765145848916No Hit
ATTAATGGGCTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAG1180.14709548740962353No Hit
ATGCGGGTGAATCTAATAATGAGGAAACATTAGAAAAAAACCAAACTGAGGG1100.13712291199202195No Hit
GCTTTAGGGATCCTGGGATGAAGTTGTTTCGTGCAGTCTATCCCTGTTTCCC1090.13587634006482174No Hit
TTAGCTGGGGATCTAAAAGTGGGAGCTGGACCCAACTAGGCCATCATGAGTG1080.13462976813762154No Hit
CTTGGTGGGGGTTCTACCAAGGCCCTTCCTTGAGCATTTATTATTTAAGTTT1080.13462976813762154No Hit
CCATAAGGGCGATCTTTTTAGGGGAAGAAGCAAAAGAAAAATTAAGAAAGTC1080.13462976813762154No Hit
ACTGGCGGGAACTTTAATACTGGACAAAATATATGAAGCATCATTTTCAGGC1060.13213662428322115No Hit
TTATAAGGGCTTGTGTATTCTAAAAATAAGAGCACAAAAACTTTGAAAGTAA1060.13213662428322115No Hit
CTGCGAGGGCTCACCAGAGGATCTTCCCTTCCTGGTCCTGCTTGTGCATATG1040.12964348042882073No Hit
GAACTTGGGACGTTATTTTACATTATTTTGTTTTTATTTTTTGTCATGTAAA1010.12590376464722014No Hit
AGATGCGGGGTTTATTGTTTAATTTATGCCCAAGTGAAGTTGTAAACTTATG1000.12465719272001993No Hit
TTGTCCGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAAT1000.12465719272001993No Hit
TACAATGGGATACCAATTTAGGGACATCGTGTTTTCCAAATCCTAAATGAAA1000.12465719272001993No Hit
ATGTTAGGGCAGTGGAAGAATTTAAATTTGGCGCTACGATCAAAACTACTGA990.12341062079281974No Hit
CATCATGGGCTGAAATTGACGTGAAGCACAGTAAATATGATTTGGTGTCTAT990.12341062079281974No Hit
TTAATAGGGTAGATATTCATGATCGCAAACATTTCCAGAGAATAATGTTGGG990.12341062079281974No Hit
AGAATAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA980.12216404886561956No Hit
AATCCTGGGCTTAAATATATTGGGTTGATCTTTTGTTTTTTAAATTAATATT980.12216404886561956No Hit
AGTAGTGGGGAAGATAACTGACAAGAAGTATTAAGAAAAGTCTTTAAGAAAA970.12091747693841934No Hit
CCACCTGGGGTCTCGCTGTTAGTGCAAGCTGATGGCTTTTGGATATGTTTTT970.12091747693841934No Hit
GGGTTTGGGATATTCTTTCTAGAAAGATGGAATTGATGTATTTTAAAATACT960.11967090501121916No Hit
CTCGAGGGGAAGAAAGCATAATCCATAGATGCTAAAACAATTAGGGGTTAAA950.11842433308401894No Hit
AACTCTGGGAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGC950.11842433308401894No Hit
TACACTGCGGGTGTTAAGTGTAATTACCCTGAAATGTTTGTAATTAATGGAG950.11842433308401894No Hit
GAACCGGGGATAACTATTAATTTTCTTTTGAAAATTTCCCCTTTCTTTGTAA940.11717776115681876No Hit
GAGTCTGGGATTATCAAGAAGCTAGGAGGAAGTTTGGCAGATTCCTATTTAG940.11717776115681876No Hit
CGCCACGGGAGGTTTTAAAAGAAATGTTATGTTTCTTATGACTTGTTTCCAC920.11468461730241836No Hit
GTCAAGGGACTAAATGGTTGCTAAAAATTACATGTCTTAAATATTGTCTTAA920.11468461730241836No Hit
ACTCATGGGGACGATGTTCTTTCTGAAATCTTCAGGGTCGGAGTCAGGCTGA920.11468461730241836No Hit
GCTCAGGGAATCTCATATAAGATAGAACATAATGATAAAAATGAGTGTTTGA910.11343804537521816No Hit
ATAGTCGGGCACTGGGGCAGGGACCATGCTGGGGCCCGGGGCCACCCCCTTC910.11343804537521816No Hit
GGCTATGGGGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCG900.11219147344801794No Hit
AGCAGTGGGGAACTGAATTGTGCTACCATCTGATTCATGCATTCAGCAAGAA900.11219147344801794No Hit
ATGCTGGGGAATGACCTCTAAGTGTTGAAGTGAAAGGAAGAATTGCACCTCA900.11219147344801794No Hit
ATGACTGGGGATGGATCTCTGTTAAAAATTTTTAGTGACATAGGAAAATGCT890.11094490152081776No Hit
ACGCATGGGAGTTTCCGTCGCCTTGACGACAAGACCACCAACGTGTCCTTGT890.11094490152081776No Hit
GGCTCGGGGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTAC890.11094490152081776No Hit
GAACGTGGTATCACCATTGATATCTCCTTGTGGAAATTTGAGACCAGCAAGT890.11094490152081776No Hit
TGGGATGGGGTAGTAAATAGACACACTGGCTTAAATAAATTATTTCTGAGGA880.10969832959361754No Hit
TCATGGGGGACATTATAAATTCACATTATGAACATTATAAGGTTTTTGAAAG880.10969832959361754No Hit
ATAACGGGTGTCCTTTTGTTAGTTTTTAGGAAGGCCTGTCCTCTGGGAGTGA870.10845175766641736No Hit
GCGCTTGGGCATATTCAACATAAATACTCTAACATAATAAATAATAAATTTA870.10845175766641736No Hit
TGTTATGGGTCTTTAAATGGCCTTTTCAAACATAACTTTTCTTCCCCATCAG870.10845175766641736No Hit
TTGTAAGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.10720518573921714No Hit
GTTTGAGGGGAAGTATTAATTTTAGAAAGATGAAAAAGGATAATAAAATTAT860.10720518573921714No Hit
GCAATGGGGTGGATACTATATCTGAAGTCAGAGCATCCACTCCAAAGCAGAG860.10720518573921714No Hit
ATAATTGGGATACAGAAATTCCATGCCTGCATCCAGTTTTCAGCAGCAAAAG860.10720518573921714No Hit
TGAGCAGGGCACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCC860.10720518573921714No Hit
TATAAAGGGTTACTGGCAGTTGTGCAAGTTGAAAGCTTATTCCTTACATCCT860.10720518573921714No Hit
CTAACAGAGGGATATTTAATCATCTCACTATCCATGTAATGTGTTCTCCAAA850.10595861381201696No Hit
AGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA850.10595861381201696No Hit
GCCCAGGGGGAATGTAAATTAGTTTAGCCACTGTGGGAAGCAGTCTGGAGAT850.10595861381201696No Hit
AGAAATGGGTCCCTGCACACAGGACTTCCTTCCATTTATTTGTGTTGCCTTT840.10471204188481677No Hit
AAAGAAGGGGTCCTTCGGCCTTGGCTCAGCTCTCCCCGGCTGGCTCCTGGAG840.10471204188481677No Hit
CTGCCGGGGTGGGGGCTGTTCTGTGGTTCCAGGTATTTTCAAGCCTGTGATT840.10471204188481677No Hit
TACACAGGGGACCAGGAACAGTTTGCTGGCATTTGAGGACGAGGAGTAAGTT840.10471204188481677No Hit
AGTCCTGGGATATTGCCTCCATTATTTCATCTGTCATATCTTGAACCTTTTC840.10471204188481677No Hit
ATGGATGGGATCTCTGGGCTAATTTGTAAGATCTATGTTTTAAAACTCCTTA840.10471204188481677No Hit
GAACAAGGGCAAATGGTATACCTGCGCCCTGCCCTCTACCTTGCCTCACAAT840.10471204188481677No Hit
TAAAGCGGGCATGTGTCTTTATAGCAGCATGATTTAACCTCAGTCTTAAGGA840.10471204188481677No Hit
TCATGTGGGCAGGATTTGAAATGTTGTCTTCTGTGCGGTGTCAGTGGGAGGA840.10471204188481677No Hit
TTGCAGGGGAGCCAGTGTGATCTGGTTCTGAAACACCACTGACTGAGGCACA840.10471204188481677No Hit
TGTGTGGGGTTCAGGATATCACTTATTAGATTGTAGACAAGGAAAATGTACA840.10471204188481677No Hit
CGACTTGGGGAAAATGACTGCTTGAGATTGCCAGTGTGAGAAGTGTGAGACC830.10346546995761655No Hit
GTTTAAGGGAATCATATTTTATATCTTCTTCGAAACCACACTTATCCCCACC830.10346546995761655No Hit
TGTGTGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGG830.10346546995761655No Hit
AACTTTGGGTAGACCTACAATTGATCTATTGAAGTTAGGACTTATTTCTTGA830.10346546995761655No Hit
TTACGTCGGGGGGCTTGTCTTTGTGTTTCTTCACTTTCCCAGTCTCCTCTTA830.10346546995761655No Hit
GTCATCGGGGTGACCTGATAGCTCCCTGGAACTTTGGGTCTGTGTGTGACAC820.10221889803041637No Hit
TAAACGGGGTGTTTGATTGAATCTGGCATCATTTCTGAGACAATTCAATTAG820.10221889803041637No Hit
TGCCTGGGCAACAGAGAGAGACTCTGTCTCAAAAACAACAACAACAACAACA820.10221889803041637No Hit
TTGCGCAGGAGGAGTTATTCATATTTATGTTGACAAAAATTCAGCTCAGGGC820.10221889803041637No Hit
GTACCAGGGGCTCTTCCGATCTTTGCAGTGGGGCCCTTCCTTCTGTTCTTTT820.10221889803041637No Hit
AGCACTGGGGGGCTCTTTGCTAGTATCTCCATATCTAGACGATGGTTTTAGA820.10221889803041637No Hit
GACAAGGGCAATCCATTATCAACTAGCTATTTCACAGGATATATTCTGTCTT810.10097232610321615No Hit
TTTTCAGGGATTGTACCACTTTTAATTGCACTTATTATTGCCTTCAGTAAAG810.10097232610321615No Hit
GGGTGAGGGACATTCAATTATGAGAGATAAACATGTAATCTCAAGTAAAAAT810.10097232610321615No Hit
AAGTATGGGTAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTT810.10097232610321615No Hit
GATACTGGGTTGCACATGTACACAGGCACTCACATGGCACACACATACACTC810.10097232610321615No Hit
TTCTGTGGGGGAGTACAAATTAGACTTATAGTCCTCCTGGAACTTGAGTTAT810.10097232610321615No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)