FastQCFastQC Report
Sat 31 Dec 2022
SRR5847615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79977
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGC4720.5901696737812121No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGCCGTCT4300.537654575690511No Hit
GCTTTAGGGATCCTGGGATGAAGTTGTTTCGTGCAGTCTATCCCTGTTTCCC1200.1500431374020031No Hit
AGATGCGGGGTTTATTGTTTAATTTATGCCCAAGTGAAGTTGTAAACTTATG1180.14754241844530303No Hit
TTATAAGGGCTTGTGTATTCTAAAAATAAGAGCACAAAAACTTTGAAAGTAA1140.14254098053190292No Hit
TTGTCCGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAAT1130.1412906210535529No Hit
ATTAATGGGCTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAG1110.13878990209685285No Hit
TGGGATGGGGTAGTAAATAGACACACTGGCTTAAATAAATTATTTCTGAGGA1060.13253810470510272No Hit
GGCTCGGGGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTAC1030.12878702627005265No Hit
AGTCCTGGGATATTGCCTCCATTATTTCATCTGTCATATCTTGAACCTTTTC1020.1275366667917026No Hit
GAACTTGGGACGTTATTTTACATTATTTTGTTTTTATTTTTTGTCATGTAAA1020.1275366667917026No Hit
CCACCTGGGGTCTCGCTGTTAGTGCAAGCTGATGGCTTTTGGATATGTTTTT1020.1275366667917026No Hit
AACTCTGGGAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGC1000.12503594783500255No Hit
AAAGAAGGGGTCCTTCGGCCTTGGCTCAGCTCTCCCCGGCTGGCTCCTGGAG990.12378558835665254No Hit
TGAGCAGGGCACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCC990.12378558835665254No Hit
TAAACGGGGTGTTTGATTGAATCTGGCATCATTTCTGAGACAATTCAATTAG980.12253522887830251No Hit
AGTGGGGGCGGTCATTTTGAGCCCCTGTCTGGATGACTTCTTGCGGCTGTTC980.12253522887830251No Hit
GGGTTTGGGATATTCTTTCTAGAAAGATGGAATTGATGTATTTTAAAATACT970.12128486939995248No Hit
ATGCGGGTGAATCTAATAATGAGGAAACATTAGAAAAAAACCAAACTGAGGG970.12128486939995248No Hit
AGTAGTGGGGAAGATAACTGACAAGAAGTATTAAGAAAAGTCTTTAAGAAAA970.12128486939995248No Hit
CTGCCGGGGTGGGGGCTGTTCTGTGGTTCCAGGTATTTTCAAGCCTGTGATT960.12003450992160244No Hit
TACACTGCGGGTGTTAAGTGTAATTACCCTGAAATGTTTGTAATTAATGGAG960.12003450992160244No Hit
TCTATTGGGATATTTGAGCAGGAGTCCTTTAGCAGATAGATGGGATGTCCTT950.11878415044325244No Hit
GGTAATGGGAGTGGAATAAAAGTTTTATGAATGGACTTTTCAACTACTTTCT950.11878415044325244No Hit
GGCTATGGGACCGATCTAATTCGGGGAACCCACAGGGGCAGAGCTTTTTAGG950.11878415044325244No Hit
GTACCAGGGGCTCTTCCGATCTTTGCAGTGGGGCCCTTCCTTCTGTTCTTTT950.11878415044325244No Hit
TCATGTGGGCAGGATTTGAAATGTTGTCTTCTGTGCGGTGTCAGTGGGAGGA950.11878415044325244No Hit
TTAATAGGGTAGATATTCATGATCGCAAACATTTCCAGAGAATAATGTTGGG950.11878415044325244No Hit
AAACGGGGGGTTTACATTTTATGAAAAGTTACATTTTCGTAATGTAACTAAT940.11753379096490241No Hit
TTAGCTGGGGATCTAAAAGTGGGAGCTGGACCCAACTAGGCCATCATGAGTG940.11753379096490241No Hit
CTTGGTGGGGGTTCTACCAAGGCCCTTCCTTGAGCATTTATTATTTAAGTTT940.11753379096490241No Hit
CTATGGGAGATAATTTTCCTTTGGGCTGCATTCATTGTTTTATAACCCTCAT940.11753379096490241No Hit
TCATCAGGGATTGGATAAATATTAGGTGGGCCTCTACTTGTATATTACCAGA940.11753379096490241No Hit
CTGCGAGGGCTCACCAGAGGATCTTCCCTTCCTGGTCCTGCTTGTGCATATG930.11628343148655237No Hit
TGTGTGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGG930.11628343148655237No Hit
ATAATTGGGATACAGAAATTCCATGCCTGCATCCAGTTTTCAGCAGCAAAAG930.11628343148655237No Hit
ACTGGCGGGAACTTTAATACTGGACAAAATATATGAAGCATCATTTTCAGGC920.11503307200820236No Hit
GACGCAGGGACACGCTGCTCGCGCTCTTAATCAAGGAACCCGGTTTGGGGCC920.11503307200820236No Hit
ATGACTGGGGATGGATCTCTGTTAAAAATTTTTAGTGACATAGGAAAATGCT910.11378271252985234No Hit
CATCATGGGCTGAAATTGACGTGAAGCACAGTAAATATGATTTGGTGTCTAT910.11378271252985234No Hit
GCCTAAGGGATAAATCCTCGCTCTCCTCTGTCTCCTGGTTTAGGTGATCTTT910.11378271252985234No Hit
GCAATGGGGTGGATACTATATCTGAAGTCAGAGCATCCACTCCAAAGCAGAG910.11378271252985234No Hit
AGCACTGGGGGGCTCTTTGCTAGTATCTCCATATCTAGACGATGGTTTTAGA910.11378271252985234No Hit
CTCGAGGGGAAGAAAGCATAATCCATAGATGCTAAAACAATTAGGGGTTAAA900.1125323530515023No Hit
GTGACTGGGGGGAATTTATGATTAGAACATTTGAAATCCAGGCAAAGTTTTA900.1125323530515023No Hit
CGAGAAAGGGGCAGGCTCTCATCGAGGCTTATGTAGACATGAGGAAGATTGG900.1125323530515023No Hit
CCATAAGGGCGATCTTTTTAGGGGAAGAAGCAAAAGAAAAATTAAGAAAGTC900.1125323530515023No Hit
TTATTGGGGGCAGTGGGGAGGGGGCTTCCCATTCAATAAATGTTACTGGCTG900.1125323530515023No Hit
TTTCATGGGAGAACAGATCTTTTACTTTGGCCGCTTGAAAAGCTAGTGTACC890.11128199357315227No Hit
CTATGCGGGTCCTATTTTTTCTGGGAGAGGGGGGGATTGATTAGAAAAAAAT890.11128199357315227No Hit
AAACGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.11128199357315227No Hit
AATACTGGAGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG870.10878127461645223No Hit
TACAATGGGATACCAATTTAGGGACATCGTGTTTTCCAAATCCTAAATGAAA870.10878127461645223No Hit
ATGGATGGGATCTCTGGGCTAATTTGTAAGATCTATGTTTTAAAACTCCTTA870.10878127461645223No Hit
GGTTCCGGGCGATCTTAGGCGGGCTTGGGTAGAATACTTAAAAGCTTATGAG870.10878127461645223No Hit
CTATGCGGGAAAATAACATTTCTTTTTGGTGTTTTAACACTTAATTTTATAA860.1075309151381022No Hit
ACGCATGGGAGTTTCCGTCGCCTTGACGACAAGACCACCAACGTGTCCTTGT860.1075309151381022No Hit
AGCAGTGGGGAACTGAATTGTGCTACCATCTGATTCATGCATTCAGCAAGAA860.1075309151381022No Hit
TAAAGCGGGCATGTGTCTTTATAGCAGCATGATTTAACCTCAGTCTTAAGGA860.1075309151381022No Hit
AAGTATGGGTAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTT860.1075309151381022No Hit
ATGCTGGGGAATGACCTCTAAGTGTTGAAGTGAAAGGAAGAATTGCACCTCA860.1075309151381022No Hit
TGCCTGGGCAACAGAGAGAGACTCTGTCTCAAAAACAACAACAACAACAACA850.10628055565975218No Hit
GGCTATGGGGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCG850.10628055565975218No Hit
CGCTCGGGGCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGC840.10503019618140215No Hit
AGAATAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA840.10503019618140215No Hit
TCGCCTGGGTAAGAAAAAATTAGAATGATGTTTGTTCATAAACTGTCTCTTA840.10503019618140215No Hit
GCATAAGGGTAAGTTCGAGGGCGGAGTGGTGATTGCCGCAGACATGCTGGGA840.10503019618140215No Hit
CATGTGGGGGAACCATGAGCCCAGATTTGCAGATAAGTGTTTAAAGTCTATC840.10503019618140215No Hit
AGAAATGGGTCCCTGCACACAGGACTTCCTTCCATTTATTTGTGTTGCCTTT830.10377983670305213No Hit
GCTCTCGGGATGTTTCTTTGTTTACTTCATTTTTTCCTCTAATTATTCAAGA830.10377983670305213No Hit
GACAAGGGCAATCCATTATCAACTAGCTATTTCACAGGATATATTCTGTCTT830.10377983670305213No Hit
ATAGAAGGGGTATCCCAAGCATTAATCAGAGATTCTGGCACATTATAAGCGC830.10377983670305213No Hit
TAAGCCGGGCAGTGTAGAATAGTGGCATAAATAAATGAATTAAGCTACATTT830.10377983670305213No Hit
TGTTATGGGTCTTTAAATGGCCTTTTCAAACATAACTTTTCTTCCCCATCAG830.10377983670305213No Hit
ATGTTAGGGCAGTGGAAGAATTTAAATTTGGCGCTACGATCAAAACTACTGA820.1025294772247021No Hit
GACACGGGAGATCAAGTATTGCCCTTTGAGAAACTGAGAATAGCTCTAATCT820.1025294772247021No Hit
TAGTAGGGGTCTGCTATAATGGCCATTTATACTGAAATTTGAGGTTACAGTG820.1025294772247021No Hit
TGCATTGGGGAAATCTTACCACCTTTCATTTTCCCTCTGTTACCAAATTTCA820.1025294772247021No Hit
AGTGCTGGGGTAGGATTACAGGCATCAGCCCACGGTGCCTGGACACCATGTA820.1025294772247021No Hit
CGTTTTGGGGAAGTGAGTGATTTTCAAACTTTTTGTATTTGGTGGGCTGTCC820.1025294772247021No Hit
ACTCATGGGGACGATGTTCTTTCTGAAATCTTCAGGGTCGGAGTCAGGCTGA820.1025294772247021No Hit
TCAGTCGGGGGAGACGCTGATGGGAGGATGGACGTACTGGTGTCTGAGTGCT810.10127911774635208No Hit
TGCGAAGGGTGCGCACGGGGAGGGTGGTTGGCCCCCATATGGGGAGAGGTCT810.10127911774635208No Hit
TTTTCAGGGATTGTACCACTTTTAATTGCACTTATTATTGCCTTCAGTAAAG810.10127911774635208No Hit
GAAACAGGGACATACCAGAATCTCTGGGACGCATTCAAAGCAATGTGTAGAG810.10127911774635208No Hit
TACGCTGGGGAAGCTCAAGTGTGGAAGCTGAGCACCTGCGACCTGTGTGAGA810.10127911774635208No Hit
GAACCGGGGATAACTATTAATTTTCTTTTGAAAATTTCCCCTTTCTTTGTAA810.10127911774635208No Hit
TTGCAGGGGAGCCAGTGTGATCTGGTTCTGAAACACCACTGACTGAGGCACA810.10127911774635208No Hit
TTCTGTGGGGAGAGCAGTGCTATTTCATCAGCATAACCCTCAACTTGAGACT810.10127911774635208No Hit
ATGTTAGGGCTTCTTGCAGAGATCTTTCACCTCCTTGGTTAGATAAATACCT810.10127911774635208No Hit
TATAAAGGGAGCCCACTGGGGTATTGATTCTCTGAAGTGAGACTAATTCAAG800.10002875826800205No Hit
CTACTTGGGAGGCTGCATTTGCATTTCTCACCTTTACAATTAGGGGCTTGTG800.10002875826800205No Hit
CATAGCGGGATTCTTCACAGAAACAGAAAAAAACAATTCTAAAATTTGTATG800.10002875826800205No Hit
GTCAAGGGACTAAATGGTTGCTAAAAATTACATGTCTTAAATATTGTCTTAA800.10002875826800205No Hit
TCTTCAGGGATGTGCTTCCTTGCTCAGTGCAAAACTGATGATTCTACTTGCT800.10002875826800205No Hit
TTTTGCGGGGTCATGACTCTCTGTTGGTAACAAGCTTTTGGGGAGAAAAAAG800.10002875826800205No Hit
GTAAATGGGAGAACATGAAATTCTGTTGGCAGAAGCAGCATTTTGAAGAAAT800.10002875826800205No Hit
GGTCTTGAACTCCTGACCTCAGGTGATCTGCCCGCCTTGCCCTCCCAATGTG800.10002875826800205No Hit
CAACTGGGTGTTGCCCGGTCTGGTCTCGAACTCCCGGCCTCAAGCAGTCCTC800.10002875826800205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)