FastQCFastQC Report
Sat 31 Dec 2022
SRR5847580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99264
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGC6690.6739603481624759No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGCCGTCT6300.6346711798839458No Hit
CGATAAGGGAGGAAGCTTTTTGGAGAAAAGAAAAAGAACAGCAGCTATTACA1460.14708252740167632No Hit
TTGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1310.13197130883301098No Hit
TCAGAAAGGGCCCAGAAAACTTGTGGGACACAGCTAAAGCACACCTGAGAAA1130.11383784655061252No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCCTGTCTCTTATAC1090.10980818826563507No Hit
TCACATGGGATATCAGGTTGGAGGTACCTATGGGACCACAAGTATACGAGTT1080.10880077369439071No Hit
TTTAGGGGGTGCCGAGAATGAGGTGGGTCATCCGAGAGGGGATTGGAATCCA1070.10779335912314636No Hit
TAAAAGGGAACTAAACTCCTGGTACTGACATTCATACGTGATGCTAAATTGG1070.10779335912314636No Hit
GCTCCCGGGGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1050.10577852998065763No Hit
AAAACCGGGATCAGTAGTCTGAATAGATGAAGTTTTCAGTTTAGGGAGTTTC1050.10577852998065763No Hit
GTTATGGGGGAAGAAGGTTGCATCACGGCTCAAGAAAAAAAAGGAAGAAGAA1050.10577852998065763No Hit
TGACCTGGGAATCATGGCTTCATATATAAATGAATGAATAAGTGGACAAAAT1040.10477111540941328No Hit
TAACAGGGGATCTTACTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT1030.10376370083816894No Hit
TAAAGCGGGGCACCAGGCATTTTTCAGGTATTGATTCATTTGATCCTCAAAA1010.10174887169568021No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)