FastQCFastQC Report
Sat 31 Dec 2022
SRR5847578.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847578.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96698
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGC6750.6980495977166022No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGCCGTCT5960.6163519410949554No Hit
CGATAAGGGAGGAAGCTTTTTGGAGAAAAGAAAAAGAACAGCAGCTATTACA1710.17683923142153923No Hit
TTGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1460.15098554261722064No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCCTGTCTCT1220.12616600136507475No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCCTGTCTCTTATAC1200.12409770626072927No Hit
TCCTTAGGGTTAAAGTGGTTTTGAATTTTTGGACAAAACTGTCTTTATTTGG1150.11892696849986556No Hit
CTCGAGTCGGGAGCCAAAGTTTAGTAAATTTATTTTGTCAAAACAATTGGTA1140.11789282094769281No Hit
CTAGCTGGGTGATGCTAAAATGAACAAGACATGCTCTCTTTCCTTAGGAAAA1050.10858549297813813No Hit
ATTGGTGGGAGCCTCCCAAGTAGCTGGGGTTACAGGCATGTGCCACCACGGC1040.10755134542596537No Hit
TGACCATGGGGTGATTCTTCTACCAAAATCAAGCTTAACTGTATTAAGCTAG1040.10755134542596537No Hit
GCTCCCGGGGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1020.10548305032161988No Hit
GCTAGTGGGTACAAAAAATAAAAAATACAGGTGGCAGGCTGGATTTGGCCTT1020.10548305032161988No Hit
ATCACAGGCGCCACCTAGTGGCAAATGTTCATGCTGGCTTAAATATCGTTTG1010.10444890276944716No Hit
TACGTAGGGCAAATCGTGGAGAGAATTATGAGTACATTGAACATCATTGAAA1010.10444890276944716No Hit
TCACATGGGATATCAGGTTGGAGGTACCTATGGGACCACAAGTATACGAGTT1010.10444890276944716No Hit
TTTAGGGGGTGCCGAGAATGAGGTGGGTCATCCGAGAGGGGATTGGAATCCA980.10134646011292892No Hit
TAACTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC980.10134646011292892No Hit
TAAAAGGGAACTAAACTCCTGGTACTGACATTCATACGTGATGCTAAATTGG980.10134646011292892No Hit
TATCCAGGGGTAAAAACAGGTTTAGGAATTCAGCAGTAAATTCATTCTTTAG970.10031231256075618No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)