FastQCFastQC Report
Sat 31 Dec 2022
SRR5847544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96074
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGC4310.4486125278431209No Hit
CTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGCCGTCT3450.3590981951412453No Hit
TCCCATGGGTAAATAACCTAATGATGCATCTCAGAGAACTAGAAAAGCAAGA1270.13218977038532798No Hit
CTAGCTGGGATGTTAAAGATTTTTGAATTTTGTTAATCTATTTTATTTTCCT1230.12802631305035703No Hit
CTTGTAGGGTGACATACGTGCCTTAAAAAGGAAAACTAGTGTTGCTATTGTG1180.12282199138164332No Hit
ACTAGCGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA1170.12178112704790058No Hit
AGTAGAGGGACTGCTTCTGTGACAAATATCGTGCCTTAACAATGTGGCTGTC1130.11761766971292963No Hit
ATAAATGGGCCTCCTCCGGCTCCTGCTGACAGCTTGGCTGTAGGAAACTCAG1130.11761766971292963No Hit
AAGCATCGGGACTATTTTAATTTGCAAAATGTTTTTTAAATCAAATATCCTG1120.11657680537918687No Hit
CCTGGGGGGGAAACCTCATTGAGATATAACAGGAGTTATTTTAAATGTCCTG1100.11449507671170141No Hit
GAATGGGAATAGAAAAACTTTTTAAACTGCCTTATCCTACTTTTAAGAACAC1100.11449507671170141No Hit
CAAATACGGGGGTATGCAGGATCCCAGCATTGATACCCAATGACAAACTATG1090.11345421237795865No Hit
ATTAATGGGGAAACTAGTTGTAACAATTTCATAACTAAAACAAATGTTGTTC1080.11241334804421592No Hit
ATATGCGGGCTGTGAAAGCAACATGAAATGAACTGTAGTAGGTGTGTAGTTT1080.11241334804421592No Hit
GTTTGGGGGATGCCTGTTTGGGTCTCATGGAATCCCAGTCTTTGAAATGGAC1070.11137248371047319No Hit
TACTGCGGGTACTTCTGGAGGAGGTGTTATGATGGATGCCTGACCCTCAGTG1070.11137248371047319No Hit
AATGCTGGTGAAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATA1060.11033161937673043No Hit
TTTACTTAATAAACTGAAAAATTTTTAGGCAGTTTATATTCCTGTTGACTAG1040.10824989070924497No Hit
GATACAGGGAGAAAATCTAAATTGTATTAGTGGTTCACATTCTATTTCTATC1030.10720902637550221No Hit
GAGTTGGGGAGCCCAGTGTTTTCTTTAGCCCCTATGATGTTCATTTTTTGTT1030.10720902637550221No Hit
GCCCACCTTGAAGGCTACAATTGAAAATAAAAATTCTGTTCTGAATACAGCC1020.10616816204175948No Hit
CCTGCAGATTGTAAGCAGGCTTGTTCAAATTCGCGATTATATTACTAAAGCT1020.10616816204175948No Hit
TCGACTGGGGAACTTTCCAAAAAAGAAATCAAGAGAACATATCATTTACAGT1020.10616816204175948No Hit
TGCTTAGGGATTTTACTGGGCCATGTTTGTTTCCTGGTACTCATGTATTTTT1020.10616816204175948No Hit
GCTTACGGGACATGGACCACGGTGTGGTCCTTCCTCACAGTTTTGCTCTTAA1020.10616816204175948No Hit
CTCTTAGGGCGGTCAAAAGAATCTGTTCCAGAATTCCCCCTTTCCCCTCCAA1020.10616816204175948No Hit
GATTTAGGGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGT1010.10512729770801674No Hit
CTATGGGCAAAAAATTGTTGAAGACGTGACTTCATAGCAATATATAGAGAAT1010.10512729770801674No Hit
AGCTCAGGGATTGCTATACATATCTGTGTGCAGGTTTTTATGTGGACTTAAA1000.10408643337427399No Hit
GCTATAGGGCAACGAGGTGATCTAGCAGATACATGGCAACGTGTGAACTGCA1000.10408643337427399No Hit
TAAATAGGGATTCCTACATGAGGCCCCAAGCAATGAATATGCAGCAGGCCAG1000.10408643337427399No Hit
TCGACTGGGACTTATTTGTGCCCAGCTTCCCAAAAAGAAAATTGTTTTGTTA990.10304556904053126No Hit
CATAGCGTGGGATGTACATTTTATAACATTAAAAAAACAAAGCATGGTAGTT990.10304556904053126No Hit
AATTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC990.10304556904053126No Hit
ACGCCTGGCCCTAGTGCTCTTATTTTGTCATGTTGGCTTTTGTTTTTAATGT980.10200470470678852No Hit
GTGTAGGGGTTTCTACTTGTTTAAAGAATCTTCTGGTCTGACCACTTAAAGT980.10200470470678852No Hit
CACGCAGGGGGAAGACCTCCCTCCTCCCAGTCCTCAAGAATCTGATGTTCAG980.10200470470678852No Hit
TATTAGGGGGATAAGAATGCCAAATAGAACTGAGTCAAAAATATCATTGATC980.10200470470678852No Hit
CCAGCCTGGGTTACAGAGACCCCATCTGAATTAAAAACATATATAATGTAAT980.10200470470678852No Hit
TTCCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA980.10200470470678852No Hit
CCACATGGGCAGTTGACAAAATAGACTACGAAAGTGGCTTTAACATATCTGA970.10096384037304577No Hit
CACAGTGGGGAGAACAATTAAGGACCTTCAAGCTAGTGAATTGTCAATTCCT970.10096384037304577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)