FastQCFastQC Report
Sat 31 Dec 2022
SRR5847543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96104
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGC3940.40997252975942733No Hit
CTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGCCGTCT3520.3662698743028386No Hit
CAAATACGGGGGTATGCAGGATCCCAGCATTGATACCCAATGACAAACTATG1290.1342295846166653No Hit
GTTTGGGGGATGCCTGTTTGGGTCTCATGGAATCCCAGTCTTTGAAATGGAC1260.13110796636976607No Hit
TAATCAGGGAGATCTTTACAGTGAGAACTGCAAAACACTGTTAAAAGAAATC1220.12694580870723382No Hit
CTCTTAGGGCGGTCAAAAGAATCTGTTCCAGAATTCCCCCTTTCCCCTCCAA1210.12590526929160076No Hit
GATTTAGGGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGT1200.1248647298759677No Hit
TTTACTTAATAAACTGAAAAATTTTTAGGCAGTTTATATTCCTGTTGACTAG1150.11966203279780238No Hit
AATTTAGGGATACTTCCTTTTATTCCTGTTAGATTCACATATATAAAAGACA1140.11862149338216932No Hit
ATTAATGGGGAAACTAGTTGTAACAATTTCATAACTAAAACAAATGTTGTTC1140.11862149338216932No Hit
CATAGCGTGGGATGTACATTTTATAACATTAAAAAAACAAAGCATGGTAGTT1140.11862149338216932No Hit
AACTCAGGGAAACAAAAGACTCGCCAGTCAATGACTTTCAAAGAGAACTAAC1130.11758095396653626No Hit
TAAATAGGGATTCCTACATGAGGCCCCAAGCAATGAATATGCAGCAGGCCAG1120.11654041455090318No Hit
TCCCATGGGTAAATAACCTAATGATGCATCTCAGAGAACTAGAAAAGCAAGA1110.11549987513527012No Hit
TTCCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1100.11445933571963705No Hit
TGACTTGGGGAGCGCACAGCGCGCCTTAGCAGCAGCAGCAGCAGCAGCGGCA1080.11237825688837093No Hit
ATGATAGGGATATAATAGGTGTATAAGGAGAAGTCATCTTACATACTAATTC1080.11237825688837093No Hit
TTGAACGGGTAGAAGTAAAAAAACAATAATGCATATTTCCTCATATCATAAA1070.11133771747273787No Hit
GAGTTGGGGAGCCCAGTGTTTTCTTTAGCCCCTATGATGTTCATTTTTTGTT1070.11133771747273787No Hit
AATGCTGGTGAAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATA1070.11133771747273787No Hit
ACTAGCGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA1060.1102971780571048No Hit
GTGCTAGGGATCTCGTTATAACTGATAGCAGAAATGGGTTTGCTGTGGCCAT1060.1102971780571048No Hit
AACGCCGGGACGCGCGTGAGGGATGTCGCGGGATCTCTATGAAGCACCAGAA1060.1102971780571048No Hit
CTAGCTGGGATGTTAAAGATTTTTGAATTTTGTTAATCTATTTTATTTTCCT1060.1102971780571048No Hit
CGCAATGGGATGCTTGGGGTTATTGGAGCCTGAGCTTGACCTCAAACGGGTG1060.1102971780571048No Hit
ACAATAGGGGCTACCGGAAGTCCGCTCTAGCTCTGGGCCCTACAACTGCACC1050.10925663864147174No Hit
CAAAGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT1040.10821609922583868No Hit
TCGACTGGGACTTATTTGTGCCCAGCTTCCCAAAAAGAAAATTGTTTTGTTA1040.10821609922583868No Hit
CATTATGGGCTGGATGGGGGAGAGAACCTTGGAATCGTCTTTGGACCCACCC1030.10717555981020561No Hit
TACCATGGGGTAATTTTAATTCAATGAAGATATCATTACCAGGTTACTCTTG1030.10717555981020561No Hit
TACCAAGGGTATCTTGTTGCTGTGAGAATTCAATGATATGACTCATGGAAAG1030.10717555981020561No Hit
TAGAGAGGGAGGTTTAAAATCTTTATTGAAGGGATCACTGTAATGTTTTACA1020.10613502039457255No Hit
GTCACCGGGACTAGAAATGGAGTTAGTAAAAGAATGAATAAACTTTGCTTTG1020.10613502039457255No Hit
GAACTAGGGAAGTAAACAGAAATCAGTACTCCGCATGCGCTCCTCTCCTAAG1020.10613502039457255No Hit
CAGTTAGGCTTCCAACTTCATGTACCAGCGTAAAGATTAAAGTGGAAAACTT1010.10509448097893949No Hit
TATATGGGGTTTGTGGCAGCTCTGCATGAAACAATTCTTTTGGCACCATTTT1010.10509448097893949No Hit
TATTAGGGGGATAAGAATGCCAAATAGAACTGAGTCAAAAATATCATTGATC1010.10509448097893949No Hit
TCGACTGGGGAACTTTCCAAAAAAGAAATCAAGAGAACATATCATTTACAGT1010.10509448097893949No Hit
GCATTAGGGTATTTCTGTTTCAGTTCTTTGGACAGTTTGCTAGTGGAAATAG1010.10509448097893949No Hit
GCGCACGGGGGGGGTAACAAACATTTCTCATTCTGTGGGTAAACAATATTAG1000.10405394156330643No Hit
CTTCCGCTGCGGGCCCAGCTGAGTCTATGTAGGTGATGTTTCCAGCTGCCAA990.10301340214767336No Hit
ATGAAGGGGCAGGAACAGTGGGCTGGAGGAAAGTTGTCAATACCTGTAAAGA990.10301340214767336No Hit
GCCTAAGGGACACTAGAGAAAGAATTTTCTGACTGTAGTTTGCAAAATAGGT990.10301340214767336No Hit
TTACATGGGATTTTAAAGAATGGTTCATTTGAGGGAAAAAAGGGTTTCTGAG980.1019728627320403No Hit
AGGACTGGGGCCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAG980.1019728627320403No Hit
CTATTGGGGACACCTACAAGATCTAAAAAACAGACTCAAAAGAGCAAATCTA980.1019728627320403No Hit
TGCTTAGGGATTTTACTGGGCCATGTTTGTTTCCTGGTACTCATGTATTTTT980.1019728627320403No Hit
TACTGCGGGTACTTCTGGAGGAGGTGTTATGATGGATGCCTGACCCTCAGTG980.1019728627320403No Hit
ACGCCTGGCCCTAGTGCTCTTATTTTGTCATGTTGGCTTTTGTTTTTAATGT970.10093232331640721No Hit
GCACATGGGTCCCAGAACAAACAGTTCATCTCCCCGTTTCTGTGCTAGTTAT970.10093232331640721No Hit
AAGGCTGGGATTCCATGAAGCCTTAAGAAAAAAAACTTTTTTTAACTTTCCC970.10093232331640721No Hit
CTCGTTGGGCGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA970.10093232331640721No Hit
AACTCCGGGAATGCCCAAAAATAGCAAGGTGGTAAAAAGAGAATTAGATGAT970.10093232331640721No Hit
GATCAAGGGATCCTGGGAGAAGAATTGCCTCTCAAAGCTGCTTCTATAAGCA970.10093232331640721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)