FastQCFastQC Report
Sat 31 Dec 2022
SRR5847542.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847542.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95886
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGC3750.39108941868468805No Hit
CTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGCCGTCT2820.2940992428508854No Hit
GTTTGGGGGATGCCTGTTTGGGTCTCATGGAATCCCAGTCTTTGAAATGGAC1170.12201989862962268No Hit
ACTAGCGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA1150.11993408839663768No Hit
TCGACTGGGGAACTTTCCAAAAAAGAAATCAAGAGAACATATCATTTACAGT1150.11993408839663768No Hit
TCCCATGGGTAAATAACCTAATGATGCATCTCAGAGAACTAGAAAAGCAAGA1150.11993408839663768No Hit
GTGCTAGGGATCTCGTTATAACTGATAGCAGAAATGGGTTTGCTGTGGCCAT1140.11889118328014517No Hit
CGCAATGGGATGCTTGGGGTTATTGGAGCCTGAGCTTGACCTCAAACGGGTG1100.11471956281417517No Hit
AGTAGAGGGACTGCTTCTGTGACAAATATCGTGCCTTAACAATGTGGCTGTC1070.11159084746469765No Hit
TGATAGGGTAAGGAAGAACTAACACAAGCTCTTTTCAAACTGTTCCAAAAAA1070.11159084746469765No Hit
ATTAATGGGGAAACTAGTTGTAACAATTTCATAACTAAAACAAATGTTGTTC1070.11159084746469765No Hit
GTCACAGGGGATGACCTGATCAGAAAATTTGTAGAAGTCTTCAAGCCAGGAA1060.11054794234820517No Hit
AATATTGGGGATGGCTGGATCATTTAAAGTTCTTTTTAGAAAATAACATAAG1040.10846213211522017No Hit
TGGAACGGGGCATTCATGCAAAAAAAACATTTAATCTGCATCTGTTTTAGAA1040.10846213211522017No Hit
TTCCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1040.10846213211522017No Hit
GCACGGGTAGCTAACATTGATTGAGCGTTTACTGTTAATGCTATTCAGGTAT1040.10846213211522017No Hit
CCTGGGGGGGAAACCTCATTGAGATATAACAGGAGTTATTTTAAATGTCCTG1030.10741922699872765No Hit
CAAATACGGGGGTATGCAGGATCCCAGCATTGATACCCAATGACAAACTATG1030.10741922699872765No Hit
TCGACTGGGACTTATTTGTGCCCAGCTTCCCAAAAAGAAAATTGTTTTGTTA1020.10637632188223517No Hit
AGTAACGGGATCTCAATAAAGAAATTGCCACAGCACTGCAGCTTCTTTTCTT1020.10637632188223517No Hit
GCTTACGGGACATGGACCACGGTGTGGTCCTTCCTCACAGTTTTGCTCTTAA1020.10637632188223517No Hit
CTCTTAGGGCGGTCAAAAGAATCTGTTCCAGAATTCCCCCTTTCCCCTCCAA1020.10637632188223517No Hit
TAATCAGGGAGATCTTTACAGTGAGAACTGCAAAACACTGTTAAAAGAAATC1010.10533341676574265No Hit
CAATTAGGGATGGCAAGCCAACGCCACTTATCCAGTGAACCACTATCACGAA1000.10429051164925014No Hit
GACTGAGGGAGTGAAATGGAAGATGCCTATCATGAACATCAGGCAAATCTTT1000.10429051164925014No Hit
AGTGTTGGGTGGTAGCAATTATTATTATAAAAGCAAAACAACCTCTTAAATA1000.10429051164925014No Hit
CTAGCTGGGATGTTAAAGATTTTTGAATTTTGTTAATCTATTTTATTTTCCT1000.10429051164925014No Hit
TTCACGGGGAGACAAGGAAATATGAACACAGACCAAAAGACTGTTAGACACT990.10324760653275765No Hit
CATAGCGTGGGATGTACATTTTATAACATTAAAAAAACAAAGCATGGTAGTT990.10324760653275765No Hit
AATGCTGGGGAGACCAAAAGATTCAAATCTCCTCTTTGTGAAATCCCAGCTG990.10324760653275765No Hit
TTTACTTAATAAACTGAAAAATTTTTAGGCAGTTTATATTCCTGTTGACTAG980.10220470141626514No Hit
GCTACAGGGTGGATAGGTTGATTCCCAACTCCCCGGATGTTTAATAGGTGAG980.10220470141626514No Hit
ACAATGGGGTACAAGGAATGATTCCAGATTCTTGCCATCAATAAGCCTATAC980.10220470141626514No Hit
GATTTAGGGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGT980.10220470141626514No Hit
GCACCTGGGCAATATAGCAAGACCTCGTCTCTGTAAAAAGAAAGAAAGTAAT980.10220470141626514No Hit
GCGCACGGGGGGGGTAACAAACATTTCTCATTCTGTGGGTAAACAATATTAG980.10220470141626514No Hit
TTTGCAGGGATTGCTTTTCTACTTATAAAAGATTACATATTCATAGTAGGAA970.10116179629977265No Hit
CACGCAGGGGGAAGACCTCCCTCCTCCCAGTCCTCAAGAATCTGATGTTCAG970.10116179629977265No Hit
TTGATAGGGAGATCACTGAGGGGGGTGAGTGTCAACATACAAGAATAAAGTG970.10116179629977265No Hit
ATACGCGGGATACTTAACTCATGGTAACATGCTTTAAAAAATTATTTTTCTT970.10116179629977265No Hit
TTATCAGGGTATTAAAATTTCTGCCATTTAGAAGAAGAGAACTACATTCATG970.10116179629977265No Hit
CATGATGGGAGTTTTGTGTAGTTCTCTAATTATCGTGAAGTGTAATAGAAAG970.10116179629977265No Hit
ACGCCTGGCCCTAGTGCTCTTATTTTGTCATGTTGGCTTTTGTTTTTAATGT960.10011889118328014No Hit
GCACATGGGTCCCAGAACAAACAGTTCATCTCCCCGTTTCTGTGCTAGTTAT960.10011889118328014No Hit
GGACTTGGGCATCCCACTTATTGCCAAAGCAGACACACTCACACCAGAGGAA960.10011889118328014No Hit
AACTCCGGGAATGCCCAAAAATAGCAAGGTGGTAAAAAGAGAATTAGATGAT960.10011889118328014No Hit
GATCAAGGGATCCTGGGAGAAGAATTGCCTCTCAAAGCTGCTTCTATAAGCA960.10011889118328014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)