FastQCFastQC Report
Sat 31 Dec 2022
SRR5847520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148068
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGCCGTCT9740.6578058729772807No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGC7800.5267849906799579No Hit
GTGCTAGGGCTAATGCACAATAAATAAATAATAAAAGGAAAAGAAGAGCTCA2020.13642380527865575No Hit
AGCATCGGGAGGGGAAAAGATTCAAGCAGTGCCATCTAGACCTTTTCTCTTC1850.12494259394332334No Hit
GTAGGTCGGGCATTCCGAATTAGCCAGTTGGGTTCAGCTTAGATTGTGCGGT1800.12156576707999028No Hit
TTAGCAGGGGATGCCTCCAGCTTTGTTCTTTTGGCTTAGGATTGACTTGGTG1780.12021503633465704No Hit
GGATATGGGAAGAACAGAAAAACAGATTAATTTTTTTGAAAAAAACAGAGAA1710.11548747872599076No Hit
TGCATAGGGATTAATGAGGAAGAAGAGATGAGAAAACAGCATTGCTGTTGAT1710.11548747872599076No Hit
TTTGCAGGGGAAAATGCTAATGAGAAACATGAGATGTAATCACTTGAAGGCA1700.11481211335332416No Hit
CGTGATGGGGAGTATTTCCAGGACAACATTCTAAAAGTACAATTATTTCTTA1680.11346138260799092No Hit
ACTGCTGGGAGTTTCCGGCAGTGTTGACTCAAACATTTGTTGAGTAACTGAG1670.1127860172353243No Hit
GCTACAGGGAACCAACTTACATTAGACAAAATATAGTTTAAGTAAAAAACAA1670.1127860172353243No Hit
AAGTTTGGGAGAATAAAGAACAATAAAGAGGAAGAAAGAAAGAGAGAAAAAG1650.11143528648999108No Hit
TCTGCTCCTCCGTTTCTTTACTTAGTTGAAATGAATAAGCCTGCTTCTCTGA1630.11008455574465785No Hit
TAGCTTGGGATGAGTCTGAATTTTACTGTGTCCCTTGCTGAAAAAAATTCAG1630.11008455574465785No Hit
TGTGCCGGGGAACAAAACAGAGAATAGGAAAAAAATAGAGAAAATGAACAAA1620.10940919037199125No Hit
GAAACTGGGGAGTGACACATCATTCACCACAATGGGTTCCTTGTGGGCAGGG1620.10940919037199125No Hit
AGCAGAGGGATACATGCTTGATTTCTACAATTCACACGTAAGTGTATTATCC1610.10873382499932463No Hit
TGAGGTGGGATTGAAGGAATTGATGCCATTTCAGCCTTTAAAACAATACATA1610.10873382499932463No Hit
TACGGTGGGGGGACTAACTCCTCCGACCTTCAGCAGATCCTGAGCCTACTCG1600.10805845962665803No Hit
TGCCAGGGGAGCCCGGATCGAAGCGCAGGGTGGCAGCCCCGGAGCCGGGGAA1590.10738309425399142No Hit
GAACAAGGGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTTT1590.10738309425399142No Hit
GGACATGGGGAAGCAAGCTGCGGATTCTGAATTTGTTAATTGCACAGCAGCT1590.10738309425399142No Hit
TGTACGGGTAGGTAGGAGGAATAATAGATGGCTGAGAGGCCAGGACCCATGC1580.10670772888132479No Hit
CCTGCTGGAGTGGCAGAAGACTTTTGAGGAGAAGCTCACGCCGTTGCTGAGT1560.10535699813599157No Hit
TTTCCTGGGAGGTTTTCTTTTCTACCCTGAGATTGAACTGTCTGATTTCAAA1550.10468163276332497No Hit
GCAATAGGGGTGGAGAGGAAGCATAACTATCAAAATTGGAGGTTCTTGTTAT1530.10333090201799172No Hit
AAGCATGGGTAACTGGAATGCTGCCTCTTGGGTTCTCACCTGCATGGATGCT1530.10333090201799172No Hit
TTTGGTGGGTTCGAGGTGGAGTCGGAGCTGGGACGGATTGTGGAAAAGGGAT1530.10333090201799172No Hit
ACTAATGGGATAGTCATCTTCTAGATTTCTAGATAAGTATCAGTTTTTTAGA1520.10265553664532512No Hit
TTTTCCGGGAGTTGGAGTTGAGCGGTGGCAATGCCTTTGCTTCATACTTTCT1510.10198017127265852No Hit
AGTGCAGGGATTCAAAGGGATAGTAACGGAACGTCCCAAACCTACAGAAAGA1500.1013048058999919No Hit
CGTTCCGGGGCATCCATCCGGCATGCCAGGTTTGGAAAGAAATCCCGGCCTG1490.1006294405273253No Hit
AGACAGGGGCACTGTGATGCATTCTCAATTTACAATAACCCTCCCTAGAAGC1490.1006294405273253No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)