FastQCFastQC Report
Sat 31 Dec 2022
SRR5847519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147412
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGCCGTCT9110.6179958212357203No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGC8110.5501587387729628No Hit
AGCATCGGGAGGGGAAAAGATTCAAGCAGTGCCATCTAGACCTTTTCTCTTC1850.12549860255610126No Hit
TGCATAGGGATTAATGAGGAAGAAGAGATGAGAAAACAGCATTGCTGTTGAT1830.12414186090684612No Hit
TTATGCGGGCAAAGGAAACTGTCCACTCTTTCTCTAAGTAATTACTTTCCCC1790.12142837760833582No Hit
GCTACAGGGAACCAACTTACATTAGACAAAATATAGTTTAAGTAAAAAACAA1770.12007163595908067No Hit
ATCTTTGGGGGACTGTCCAAGCAGATAGGCTCACACGAGAAACAGTGAGGCT1740.11803652348519796No Hit
TTAGCAGGGGATGCCTCCAGCTTTGTTCTTTTGGCTTAGGATTGACTTGGTG1730.11735815266057037No Hit
AAGCATGGGTAACTGGAATGCTGCCTCTTGGGTTCTCACCTGCATGGATGCT1730.11735815266057037No Hit
ACTGCTGGGAGTTTCCGGCAGTGTTGACTCAAACATTTGTTGAGTAACTGAG1720.1166797818359428No Hit
AGTATGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAG1700.11532304018668765No Hit
AGTCTAGGGGTTTAAGAAAAATATACTGAATTTATAGAAAGCAAGTATTCTC1680.1139662985374325No Hit
GGATATGGGAAGAACAGAAAAACAGATTAATTTTTTTGAAAAAAACAGAGAA1660.11260955688817736No Hit
TCTGCTCCTCCGTTTCTTTACTTAGTTGAAATGAATAAGCCTGCTTCTCTGA1630.11057444441429462No Hit
GTGCTAGGGCTAATGCACAATAAATAAATAATAAAAGGAAAAGAAGAGCTCA1630.11057444441429462No Hit
CAATAAGGGGTTAGCCCTTCTGAATGTTTGTCAATTGTGTTTATTTTTTCAA1630.11057444441429462No Hit
GACTCAGGGGCTTCTGGTGCTCTAGGGTGAGCTCTGCCCGGCTGCAGGGATG1620.10989607358966706No Hit
TACCTAGGGACTACAGCATAAATCTGTTAGCATTATTGCCAAATAAGACAGT1620.10989607358966706No Hit
GTAGGTCGGGCATTCCGAATTAGCCAGTTGGGTTCAGCTTAGATTGTGCGGT1620.10989607358966706No Hit
TGAACTGGGTCTCGGCTCACTGCAACCTCCGCCTCCCAGGTTCTCAAACAGA1600.1085393319404119No Hit
TTGTTCGGGTAAGAACTTTGCAAATACAAACTCCTTAATCCTCCAAGCGACC1600.1085393319404119No Hit
TGAGGTGGGATTGAAGGAATTGATGCCATTTCAGCCTTTAAAACAATACATA1590.10786096111578435No Hit
AACCAAGGGGTCGGTTAGTTGAGATAGGATGTGGTCTTAAGTAGTAAACAGT1590.10786096111578435No Hit
AAGTTTGGGAGAATAAAGAACAATAAAGAGGAAGAAAGAAAGAGAGAAAAAG1560.1058258486419016No Hit
GACGAGGGGCAGAGAAACTGGGCAACAATTTGCTGTAAAAATTGTTGATGTA1560.1058258486419016No Hit
CAACATGGGAGCCTCTCTCAGTTGTCACAGGACTGAAGAGAGTTAGGGCCTT1540.10446910699264646No Hit
GAGGTTGGGGCAGCCTTTAATTTGCTTAGAAGGCAACATTAGAAGGTTAGAG1520.10311236534339133No Hit
GCCTACGGGACAGGTGTGAGCCACTGCGCCCTGCTCGATTATTTTCATATTT1520.10311236534339133No Hit
ACTAATGGGATAGTCATCTTCTAGATTTCTAGATAAGTATCAGTTTTTTAGA1520.10311236534339133No Hit
TGGCGTGGGAGCCCAGGATTGCTACAAAAAGGTCCTTTGAGAAAATTAATTT1520.10311236534339133No Hit
GAAACTGGGGAGTGACACATCATTCACCACAATGGGTTCCTTGTGGGCAGGG1520.10311236534339133No Hit
GGGGCAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1510.10243399451876373No Hit
TAAGCAGGGGCATCCATTTACTATGATTCCGTTAATTTGTTGAATTAAATGC1510.10243399451876373No Hit
GTTCTTGGGACCCTTATGAGATCTCCAGAACTAGACGCCATGGCTGCATTGA1500.10175562369413617No Hit
TTTACAGGGTGGTCTCTTCCAACTTCATGTACCAGCGTAAAGATTAAAGTGG1490.10107725286950858No Hit
GATAATGGGAGGTAAATGTTACTTTTATCCTCAATTCCTTGATGAAGAAACT1490.10107725286950858No Hit
GTTCAAGGGAAGCTATTTCATGCAAACATTATCTAATTTAGCTTAAAAGTGA1490.10107725286950858No Hit
GGACATGGGGAAGCAAGCTGCGGATTCTGAATTTGTTAATTGCACAGCAGCT1490.10107725286950858No Hit
TAGCTTGGGATGAGTCTGAATTTTACTGTGTCCCTTGCTGAAAAAAATTCAG1480.10039888204488101No Hit
ACGTTGGGGGGAAAGGATGAAACTAGAACAAGAACGACAACGTCAACAAAAA1480.10039888204488101No Hit
ATGCGGGGACAGTGTTTGAGCTAGAAAAGTTGATAAACTTATATAGCATTTC1480.10039888204488101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)