FastQCFastQC Report
Sat 31 Dec 2022
SRR5847518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145171
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGCCGTCT8640.5951601903961535No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGC6900.4753015409413726No Hit
GTGCTAGGGCTAATGCACAATAAATAAATAATAAAAGGAAAAGAAGAGCTCA1890.13019129164915857No Hit
GCTACAGGGAACCAACTTACATTAGACAAAATATAGTTTAAGTAAAAAACAA1760.12123633508069794No Hit
TTAGCAGGGGATGCCTCCAGCTTTGTTCTTTTGGCTTAGGATTGACTTGGTG1750.12054749226773943No Hit
AAGTTTGGGAGAATAAAGAACAATAAAGAGGAAGAAAGAAAGAGAGAAAAAG1740.11985864945478092No Hit
TGCATAGGGATTAATGAGGAAGAAGAGATGAGAAAACAGCATTGCTGTTGAT1710.11779212101590537No Hit
TCTGCTCCTCCGTTTCTTTACTTAGTTGAAATGAATAAGCCTGCTTCTCTGA1700.11710327820294687No Hit
AGTCTAGGGGTTTAAGAAAAATATACTGAATTTATAGAAAGCAAGTATTCTC1690.11641443538998836No Hit
TACGGTGGGGGGACTAACTCCTCCGACCTTCAGCAGATCCTGAGCCTACTCG1670.11503674976407134No Hit
ACTAATGGGATAGTCATCTTCTAGATTTCTAGATAAGTATCAGTTTTTTAGA1620.11159253569927878No Hit
CATCAGGGGACTTTGAAAATTTCTCTTCTATAGTGTGTTTGTTTGATTTTAA1600.11021485007336176No Hit
AGCATCGGGAGGGGAAAAGATTCAAGCAGTGCCATCTAGACCTTTTCTCTTC1590.10952600726040324No Hit
GCTAACGGGCTGACAACAAATACATCATTCTGCAAAAACTGGCAAATGTGTT1580.10883716444744475No Hit
GAACAAGGGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTTT1550.1067706360085692No Hit
CTGGCTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1540.10608179319561069No Hit
TTACACGGGTCAAACAAAAGACTTGCTTCAAGGTGTTTTTAAATAGCAGTGA1530.10539295038265219No Hit
CGTTCCGGGGCATCCATCCGGCATGCCAGGTTTGGAAAGAAATCCCGGCCTG1530.10539295038265219No Hit
TATACAGGGATTTGGAAGTTTTTTACATAGGAGGTACCCAATAAGGATGTTT1520.10470410756969366No Hit
TTTTCCGGGAGTTGGAGTTGAGCGGTGGCAATGCCTTTGCTTCATACTTTCT1520.10470410756969366No Hit
AATGTCGGGACTGCCGTCCTATTTCTCATCCTGAGCACTCAGAAAGACTGCA1520.10470410756969366No Hit
GTGATGGGATTTTTTGACCCTCTGAAAATTATTATACTTCACCTAAATGGAA1520.10470410756969366No Hit
ATGCGGGGACAGTGTTTGAGCTAGAAAAGTTGATAAACTTATATAGCATTTC1500.10332642194377664No Hit
AGCTGGGAACCAATGAGAACAAAGACACAATGTACCAGAATCTCTGGGACAC1480.10194873631785963No Hit
TGTACGGGTAGGTAGGAGGAATAATAGATGGCTGAGAGGCCAGGACCCATGC1480.10194873631785963No Hit
TTCAGCGGGTGACTCAACTGTGGCGATGTGGACGGAGTCACCGAGCTGCTTT1480.10194873631785963No Hit
ATAATCGGGAGGAGAGCAGCGGGGCGAAGCCCAGGCCCCTTTTCACTCCCTC1470.10125989350490111No Hit
TCACATGGGCCTTACAGAGGAGCTGGATATATTCGTTAACATTATGCACATT1460.10057105069194261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)