FastQCFastQC Report
Sat 31 Dec 2022
SRR5847514.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847514.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136714
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGC7600.5559050280146876No Hit
CTTATACACATCTCCGAGCCCACGAGACACCCGTAAATCTCGTATGCCGTCT7440.54420176426701No Hit
ACTCTTGGGTATCTCCCTCTTCGAGACCGCCACGGGCGAGCTGATGATTTAC1740.12727299325599428No Hit
GAAACAGGGATTTAGTCAATTCAACAATTTACAGGGCTGAAAATATTTATTG1550.11337536755562708No Hit
GTCAGGGGGATGCATCGCTGCTTCGTTGTCTGATTTTGCTTAGATTTTGCTT1540.11264391357139723No Hit
CAAGCGGGTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCT1420.103866465760639No Hit
TATAAGGGGAGGGGCGGGGCACCGGCCTCTGGGCGGAGATCTGCTGCCGCGT1420.103866465760639No Hit
TAGCATGGGGCCACTGCACCCAGCCTATTTCATAAATAGTGGATTGCACAGC1390.10167210380794944No Hit
AAGCCAGGCACGTGTGTGAAGGTGCTGACCATCGAGCCCCCACCTGCAGACC1380.10094064982371959No Hit
GCGAACGGGAGAATTAAGTCAATACTTCTACATGCATTATAGACCAAAGGTC1370.10020919583948974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)