FastQCFastQC Report
Sat 31 Dec 2022
SRR5847490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108486
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC6880.6341832125804251No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT5170.47655918736058106No Hit
GAGCCTGGGAGAGGTCAGTGCTCACCAAACATCAGAGAATTCACACAAGGGT1800.16592002654720425No Hit
CTACTCTGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAA1700.1567022472945818No Hit
TTCAGAGGGGTGAAAATGGGATTGGGAAGCGGTATAAAGGAATTTGAAATTT1580.14564091219143485No Hit
GATCTCGGGAAAGTCTTTTGGGGAAGACTGGCAATGTGCTTGAAGACCTTCA1580.14564091219143485No Hit
GAGTCTGGGAACTCGCAAAGTAGAATCCTTACCTACTACTCTTCTGATAATA1570.14471913426617258No Hit
AGCTTGGGGGAGGTATCACAGGTACATTGGAAAGAACATGAAATTTGAAATA1540.14195380049038586No Hit
GTTAATGGGACCGTGCCTGGCCTAAACCTTACGCTTTTGAGGTTGAGTGCAG1500.13826668878933687No Hit
GTCCTGGGGATCGGACCTCGGCCAGTGTGAGCGGCAAGAGGATAGGAGATTA1500.13826668878933687No Hit
TGAAGTGGGTGTCTCTTTCCGAGAGTAATGATGAAAACATTGAAGAAACCAT1500.13826668878933687No Hit
TCTATGGGGCATGTGCCATATTATGCCTTTTCTTCAATGTGTGTCAGTTTTG1500.13826668878933687No Hit
TTGCAGGGGCCCGATAGTAAAGAATTTTCTGGCTCAAAATGTTGACAGTATT1480.1364231329388124No Hit
GAAGCTGGGATAACATTAGAATGAGTAGCTGTAGTTGTGTGGACATTTATAA1450.13365779916302564No Hit
TACGAGGGAACCCAATCTCTAGAGTTGCTAAGCTTCTAAAAGCCTTAAATGT1440.1327360212377634No Hit
GCTATAGGGACTGATTCCAATTTATTGCCTGTACCACATTATTCTTATTTTT1430.13181424331250116No Hit
ATATGAGGGAAGGCCCAATGTTATGGCCTAATGAAAGCTATATACTTGTGCT1420.13089246538723892No Hit
TAGATGGGGAGACATGGCTTTGAAAATAATTAAATGCTTTGCTAATAGACAA1400.1290489095367144No Hit
TCATCTGGGGTACTTGTGGAAACTAATCCCTAAATATGAATAGGTTTATATT1400.1290489095367144No Hit
TATTAAGGGTGTTTGTGTGTCCTGGATTATCAGAGAAGCAGTACTTGAAAAT1380.12720535368618993No Hit
AAATGGGGAAGACTAATATTTTACTTGATTTAGGAGGAATGCAGTTCAAAAT1370.1262835757609277No Hit
TATGCAGGGGGAAAACAGCATGGATGTTCCTAAAAAAATTAAAAATAGAACC1370.1262835757609277No Hit
ACGCGGGTAATGTAGAAAGCCACTAAAAAGTCTATTTGAAATTGCTCTTCTT1360.12536179783566542No Hit
ATCTTTGGGGATTTGATTTCTTAGTCAGATGCTGTGGTACTTGGCATTTAAC1360.12536179783566542No Hit
CAGTTTGGGATATTCATTTAGCATTAAGTCAAGTAGACTGAGAATGACTCAA1360.12536179783566542No Hit
AGTTGAGGGATTGTATAAATAATATGCTTTGAGTGGCAGGAAATAAAACTGA1360.12536179783566542No Hit
TGACAAGGGGAAATTTGCATGTGACCTCATCTAGCTATGAATTCTGGGAAGT1340.12351824198514093No Hit
ATAGCGGGGGTTCTGGTTAGTGTAGAATTGTTCCAAAAGAAAATAGGTCAGC1330.12259646405987869No Hit
GCACAAAGGGACCCTGAGCCATACGGAAACAACTTGAACTGTATATGGAAGA1330.12259646405987869No Hit
GTTTCGGGGCAAACATATTGGTACGCATAGTAAAGTGCATCCTATTGCCAGT1330.12259646405987869No Hit
TGATCAGGGAGTGAAAGTGAATTACTTATACAAGATTCTAGTTTTACCTAAA1320.12167468613461645No Hit
AAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1310.12075290820935419No Hit
TAGCCTGGGCAGCAGAACGAGACTCCGTCTCAAATAAATAAATAAATAAAAT1310.12075290820935419No Hit
GGTGTTGGGGGAAAACTTGGTTTCAATGATGCCCGTGGTTTTGACTCTGGAT1310.12075290820935419No Hit
GATTAAGGGAGCTAAAGAAATAAATTGGTAACAAAATAAAAATAAGAATGCA1300.11983113028409195No Hit
CTTGTTGGGATGTTTTCAAAGCAAACAATAGAATTCAAGAAAATGCTTAAAA1300.11983113028409195No Hit
GCTAACGGGGGAAACAGAAAGCAAACTGATAAAAAGCCAGTGAAGACAAAGA1300.11983113028409195No Hit
TGCTACCGGGGTCTCTAGGTGGAAAACAGCCTCAGGCCAAATAGGGAAGAAG1300.11983113028409195No Hit
TGACTTGGGGGGCTAAGGACTTGTTCTATTATAGCACAGTAATCATTTTACT1300.11983113028409195No Hit
AGCCGAGGGAGTGTGAAAATTGCATTTTTCTTTATTTTATATGCCTGCATTT1300.11983113028409195No Hit
CAATCAGGGGCTTGATATAACTTCAAAGCCGATCTGGGCTGAGTCTTTTGGA1300.11983113028409195No Hit
GATAATGGGACTGGTAGTATATGCTGTTTTCTGCTGTCTTTATCCCAAGTTT1300.11983113028409195No Hit
GCCGCAGGGAGATTCCTGCCAACTCAAAATACAGTACAGCTAGTAAAATGTT1290.11890935235882971No Hit
CTCTCGCGGGACCCTTTGCCTGCCCCGAGTGTGGCCAGCGCTTCAGCCAGCG1290.11890935235882971No Hit
ATTAAAGGGCAACAAAGAGAGGTTCCAGAATTTTGGCCTAAGCAGAAAAATA1290.11890935235882971No Hit
TAGCACGGGGACATCAGCAGTTGACACCTTCCCAGGAGCTGGATGATCTGAT1280.11798757443356746No Hit
ACACGAGGGTAGTGGTGCCATTGAATGATCTTCCAACACAGAGGCGATCACC1280.11798757443356746No Hit
CTAATGGGGTTAGAATGGCGATCATTAAAAAGTTGGGAAACAACAGATGTTG1280.11798757443356746No Hit
TCGCGGGAGAAGAAATTAGAGAAGCATTCCGTGTGTTTGATAAGGATGGCAA1270.11706579650830522No Hit
GCTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.11706579650830522No Hit
ATTAAGGGGAGGTACTACCAAGGGGGGAAAATAGATAAATCTAATTGACCTA1270.11706579650830522No Hit
CTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1260.11614401858304298No Hit
TTTCGCGGGCAAGCTCTCCAGATGATCTGAGGCAGGTATCTGAAGGGCTGTT1260.11614401858304298No Hit
TTTATAGGGGTTTCGGTGGGAATGACAACTTCGGTCGTGGAGGAAACTTCAG1260.11614401858304298No Hit
TGTTAGGGGATAATGACTTATGAATTAGCACAGTTAAGTTGACACTAGAAAC1250.11522224065778072No Hit
GCTCTCGGGCATTTTTTAAATTGAGGTAAAATTCACATAACATAAAAGTAAC1250.11522224065778072No Hit
TGATTGGGGTCTCAAGGTGGGTAGCTGTTTTACAATTAATTATCCAGTTACT1250.11522224065778072No Hit
AGGAAAGAAATCAGTGTAATAATGCTGAAGTATTTAATAGCAAAGGGTTATG1240.11430046273251848No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAG1240.11430046273251848No Hit
ATACACGGGCATTGCAGGATTTTAAGCTTTAATATATTTGCTGTTTAGACAC1230.11337868480725624No Hit
CTCACAGGGGAGCCCGATTAAAAACAGTGAAATTCAATTCTAAAACGAGAGA1230.11337868480725624No Hit
TGTTTGGGGACTTCTGCTTCCAGTGAAGCTGGCATAACAGAGACCAGGTTTA1230.11337868480725624No Hit
CTTGTCGGGAAGTTTTCTCTGTATCAAGTGCAGGAAAGGAACATGGGTAGAG1230.11337868480725624No Hit
AGGCTCATCCCCAATACCACCATGCTATGCTATGAATAATAAAGTCCTTTGT1220.112456906881994No Hit
AGTGTGGGGAGGCGAGTCATACTCTTGCAAGTAGTTAGCAAAGGGAGGCCCA1220.112456906881994No Hit
GCTGGAGGGAACTTAGGTAAGGTAAGGTAAAATAACCCTTTAATGTAGTCAT1220.112456906881994No Hit
CACGTTGGGTAATGAGGCCTTATTGTCAACCCTCATAAATTCAGAGTATTTC1210.11153512895673175No Hit
TAAGCTGGGGAATAGAATTCGTTCAGCCGAACGAATGAGAATGGATGATTTT1210.11153512895673175No Hit
AAATGCGGGACAGGGTTTCAACATCAAAAGTGTACAATCACAAGGTTTTAAA1210.11153512895673175No Hit
ACAACTGGGAGCCATTGGTTGCTGTTCAGGTCAGCTTTGCTCCTAACAACAC1200.1106133510314695No Hit
TTGGGTGGGCAGGAACCAGGCCACAGAAGAGCGTAGCATACTCTACTAAAAC1200.1106133510314695No Hit
TTAATGGGGAATCCAGAGGAATTCTCACACACAAGGCAAGACGTATGAGGAT1200.1106133510314695No Hit
CTCAGCGGGAAGCGCAAAAAGTATTTCCTCAGACGTTTTGGTCCACTGACAT1200.1106133510314695No Hit
GTTCCAGGGAACTTTGTAATATTTTGGTTAGAAATATTTCAGTTAACTCCAG1200.1106133510314695No Hit
TCATAAGGGCCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGG1190.10969157310620727No Hit
GCTTTTGGGGGAGATAATTAGTCAAAGGGCACATGGAGTATGGAAGGGGGCA1190.10969157310620727No Hit
ACAGAGGGGGAGAAGTGAGCTGAGCGTGTGCGCGGTACGGGGCTCTCCTGCC1190.10969157310620727No Hit
TGTCACGGGGGTATCACAGCGTGCCACACAGGGATTTACGGTGGTCAAGAAG1190.10969157310620727No Hit
AGCAACGGGATTCCTATCTGATGATTTTAAATGGAACACTTAGAGAAGTAGG1190.10969157310620727No Hit
TTTGCCGGGGAGGTGGTATGGATCAACAAATGGACCCACAGATTTTTCTTAT1190.10969157310620727No Hit
ACTCTTGGGCAGCAAGTGGCAGGAGCAGGCGCTGGAGCAGGCCCCGAGGGAG1190.10969157310620727No Hit
GTCACGGGGATACCAAAGCTTGTTTATTTCTGATAATTAACCTAAGCCCTTA1190.10969157310620727No Hit
GTTTATGGGACCGTAGGGCTCTGGAAGGGATTAATAAAATAATATGAGTAAA1180.10876979518094501No Hit
TATGCTGATGATGTAGAAGAGTATATGATTGAACGACCAGAGCCAGAATTCC1180.10876979518094501No Hit
TTTGACGGGAGTGCTGCAGAACTTCGCCATGTGATGACAAACCTTGGAGAGA1180.10876979518094501No Hit
ACATCAGGGGACCTATGGTGGTGACCAATGCATTGGTTACAGAAACTTTAAG1180.10876979518094501No Hit
ACACTCGGGAGCTCAGATCTCCTGAGACTCAATTGCCTATTCTGTTAAAGGG1180.10876979518094501No Hit
AGTTGCGGGGAATCTTCCAATTTTATCTATTTGATTCAAATGGTATGTTTTT1170.10784801725568277No Hit
TTTGTTGGGTCTTCGGGTAAAGATGGCGGAGCGCGGGTACAGCTTTTCGCTG1170.10784801725568277No Hit
GCTGCCGGGGATCTTAGAATGGGATACTAAAAACATGACTTGTTAGTTGTAT1170.10784801725568277No Hit
AACTGTGGGATCCCTTGCTGGTGATACCAGCAATAGTTCTTCCCCGGCCTCC1170.10784801725568277No Hit
AGCTACGGGATGCAAATAAGACAGTGGAGCTAAATTTTTTAATTAATGAAAG1170.10784801725568277No Hit
TAAATTGGGTACTTAAAGGAACTGATTTTTTAAAATTGGATTGAATCATGGA1160.10692623933042053No Hit
ATTAATGGGTATGATGATATGATGATGATGGATGAATTTTATATATGAAAAA1160.10692623933042053No Hit
GCAATGGGCGATCTTTAGCGGGCTAACTGGAGAGAAAAACAGACCAGGAGAG1160.10692623933042053No Hit
TGTTAGGGAGCGACACCACAGGCAAGCTGGGCTTTGAGGAATTCAAGTACTT1160.10692623933042053No Hit
CGCAGGGGGCAAGGAGTGCAAGCTTACATAAATTGTAAATATGATGCATCTA1160.10692623933042053No Hit
CTACATGGGCTGACAAGGGTCTTCTTGCTGCATCATCCCATGGCTGAAGTGG1160.10692623933042053No Hit
AAGCTAGGGGGGTTTTAAGGGGTGTGGCAGGAGGTTTTGGACTCGATGAGTT1160.10692623933042053No Hit
CGAATAGGGACGCATTTTGTTTTGCAAAATGAGAAGAGCTTATAAATACTTC1150.10600446140515826No Hit
AAATTAGGGAGGAACTGTGTGTGTCTTAAGACCGAAGTTCAATATGGTATTT1150.10600446140515826No Hit
CGAGTTGGGAAAAACCAAACTATTAAAATCCTCCTATAAATATTCTTTTTCT1150.10600446140515826No Hit
GATGAGAGGGAGAGTGCGGTTGTACATCCTCCCCACCTCATAACGCAGCCAC1140.10508268347989601No Hit
GGTGATGGGGTTCTAATAGAAGACTGTATCCCGGTACTGAAGAGGTACGCCA1140.10508268347989601No Hit
GTGTCGGGGGGGAGTAAAAGTATCCAAATAACAACAACAAAAAAAGCATCTG1140.10508268347989601No Hit
GTGACTGGGACCTACGGAAAGTCTAAAGTATTCTGGATTGTCTTTAATATTG1140.10508268347989601No Hit
GTGCTCGGGCGATCTCGTCACGGGACAGTGAAAGAGTAAATTAGAAAGTGAA1140.10508268347989601No Hit
GAACATGGGAGGAAGAACCAAGGTACTTTTTTTGTAGGCAAAGGAAAAGTAT1140.10508268347989601No Hit
GTAGTGGGGATGGGCTTTGGTGGCGCCTCTTTCTTGGTTTCCTGCTGCCCTT1130.10416090555463377No Hit
AAAGCTGGGTCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAA1130.10416090555463377No Hit
TCGAGGGTGGAAGTTTACAGCTATTACCAGGGGGCTAACTCCCGGGGCATCG1130.10416090555463377No Hit
TGTCAAGGGGGGTGGGGTCTTTCCCTTTACAGACGGGGCAGATGCCAGGACT1120.10323912762937153No Hit
ACAGAGGGGTGTCCAAAAGGTTTTCCAAATCTTATAAACTGTAAGTCATTGA1120.10323912762937153No Hit
ACACCAGGGTTACAGGCCTTGACGTGTTAATGATTTCTGGCTTTCCTGATGG1120.10323912762937153No Hit
GTTCTAGGGGACACAGAGAGAGACATTGAGATGAAACGGCAACTACGGCGAC1120.10323912762937153No Hit
AACCCTGGGAATTTAAACAGGGAAGAGGTTTAATGGACTCACAGTTTCACAT1110.10231734970410927No Hit
TTTAAGGGGCTGTTTAATGGTCTTGGGCTTGGAGCAGTAATTGGCCTGGGAG1110.10231734970410927No Hit
ATTCTGGGGCTTGGAAAATTTTGGGTTGCTATGGTGTCTTTAATTTCCTCTT1110.10231734970410927No Hit
CACTTGGGACTTACATAGCAAGATAATGCTAATTTGCCATTTTATCTCAAAT1110.10231734970410927No Hit
GTTCTAGGGTAGTTATAATCCTGTTTTATTATCCATTTGTTATGTGATTACG1110.10231734970410927No Hit
ACTGCAGGGCTACTTATTTACTTCCTTTCTCTTCTCAAAGATAATACATGCG1110.10231734970410927No Hit
TAAAAGGGGGCCCTCAGTGCAACACGATCACAGCGCCCGGAGACCTGCGGAG1110.10231734970410927No Hit
GTCCTGGGGATGACAAAGTGTAATTAACACTGATGTTTGTGTTAAATTTGCA1110.10231734970410927No Hit
AGAACCGGGAGGGCCAAGCATTTTGTTTGTTGGTGTCCTCGTGTCTTCTGGG1110.10231734970410927No Hit
ATATTTGGGATGAACACAAAGGAGCTTTTAAGAGATGTCCATACCCTCCTTT1110.10231734970410927No Hit
ATCGTTGGGAGGAAAAGGAATAGATTTCAGAGCTTATTTAGGAGATAAATTA1100.10139557177884703No Hit
ACTACACGGGTTGGATTGTTCCCTGGAGCCCCTCATTGATCCTTTGAACTTA1100.10139557177884703No Hit
AAATCAGGGGATCTGATCGCGGGAAACTCCAGCATCACGCAATTTTTGCTTT1100.10139557177884703No Hit
GACACTGGGTACCAGGACATAATTTTTGTCTACGCACTTCTCTCTCATTGAC1100.10139557177884703No Hit
ATGTTTGGGGGGCTGTTTTAACCATGAAAGGACCATAAGAAAGGCAATTCAC1100.10139557177884703No Hit
GAGTGGGACTTGTCTTTGTGATGTTATACATGGAAGTGGAAAAATGTATTTC1100.10139557177884703No Hit
ACAGAAGGGCTAAATGACTTTTGACATACAAACTCTTCTTGAGAATGTTTGT1100.10139557177884703No Hit
CCTCCTAGGGGCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGG1100.10139557177884703No Hit
GTTCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1090.10047379385358479No Hit
GCATTTGGGCATGCCCGCCCGCATAGGACTGATGCTTCATCTGGACTTGAAA1090.10047379385358479No Hit
GTGCCGGGTGCATTGTTGGGGGTCAGAAAATGATACCCCATAATATGGCACC1090.10047379385358479No Hit
TAGGTGGGGATATTTAACTTTAAAGATTCAAGGAAATGATATTGTAAATAAT1090.10047379385358479No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)