FastQCFastQC Report
Sat 31 Dec 2022
SRR5847489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108484
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC7180.6618487518896795No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT5390.4968474613767929No Hit
GAGCCTGGGAGAGGTCAGTGCTCACCAAACATCAGAGAATTCACACAAGGGT1790.1650012905128867No Hit
TGACTTGGGGGGCTAAGGACTTGTTCTATTATAGCACAGTAATCATTTTACT1690.15578334132222263No Hit
CAGTTTGGGATATTCATTTAGCATTAAGTCAAGTAGACTGAGAATGACTCAA1650.15209616164595702No Hit
GTCCTGGGGATCGGACCTCGGCCAGTGTGAGCGGCAAGAGGATAGGAGATTA1640.1511743667268906No Hit
GTTAATGGGACCGTGCCTGGCCTAAACCTTACGCTTTTGAGGTTGAGTGCAG1610.1484089819696914No Hit
ATTAAAGGGCAACAAAGAGAGGTTCCAGAATTTTGGCCTAAGCAGAAAAATA1610.1484089819696914No Hit
TTGCAGGGGCCCGATAGTAAAGAATTTTCTGGCTCAAAATGTTGACAGTATT1590.1465653921315586No Hit
GCCGCAGGGAGATTCCTGCCAACTCAAAATACAGTACAGCTAGTAAAATGTT1590.1465653921315586No Hit
GAGTCTGGGAACTCGCAAAGTAGAATCCTTACCTACTACTCTTCTGATAATA1560.14380000737435936No Hit
GATCTCGGGAAAGTCTTTTGGGGAAGACTGGCAATGTGCTTGAAGACCTTCA1560.14380000737435936No Hit
TATGCAGGGGGAAAACAGCATGGATGTTCCTAAAAAAATTAAAAATAGAACC1520.14011282769809372No Hit
CTACTCTGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAA1480.1364256480218281No Hit
GTGCTCGGGCGATCTCGTCACGGGACAGTGAAAGAGTAAATTAGAAAGTGAA1470.1355038531027617No Hit
GTAGTGGGGATGGGCTTTGGTGGCGCCTCTTTCTTGGTTTCCTGCTGCCCTT1460.13458205818369529No Hit
TTCAGAGGGGTGAAAATGGGATTGGGAAGCGGTATAAAGGAATTTGAAATTT1460.13458205818369529No Hit
GTTTCGGGGCAAACATATTGGTACGCATAGTAAAGTGCATCCTATTGCCAGT1450.13366026326462888No Hit
AAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1440.1327384683455625No Hit
ATTAATGGGTATGATGATATGATGATGATGGATGAATTTTATATATGAAAAA1440.1327384683455625No Hit
AGGAAAGAAATCAGTGTAATAATGCTGAAGTATTTAATAGCAAAGGGTTATG1410.12997308358836326No Hit
CTAATGGGGTTAGAATGGCGATCATTAAAAAGTTGGGAAACAACAGATGTTG1410.12997308358836326No Hit
ACACCAGGGTTACAGGCCTTGACGTGTTAATGATTTCTGGCTTTCCTGATGG1400.12905128866929685No Hit
TAGATGGGGAGACATGGCTTTGAAAATAATTAAATGCTTTGCTAATAGACAA1390.12812949375023044No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAG1390.12812949375023044No Hit
ACAGAAGGGCTAAATGACTTTTGACATACAAACTCTTCTTGAGAATGTTTGT1380.12720769883116403No Hit
TTTCGCGGGCAAGCTCTCCAGATGATCTGAGGCAGGTATCTGAAGGGCTGTT1380.12720769883116403No Hit
CCTCCTAGGGGCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGG1380.12720769883116403No Hit
ATTAAGGGGAGGTACTACCAAGGGGGGAAAATAGATAAATCTAATTGACCTA1380.12720769883116403No Hit
TGATCAGGGAGTGAAAGTGAATTACTTATACAAGATTCTAGTTTTACCTAAA1370.12628590391209762No Hit
TCGCGGGAGAAGAAATTAGAGAAGCATTCCGTGTGTTTGATAAGGATGGCAA1370.12628590391209762No Hit
GTGCGTGGGAAAAAAGGGATGAGGTAAGTTAGTATGGAATGAACATTTCTTG1370.12628590391209762No Hit
TAGCCTGGGCAGCAGAACGAGACTCCGTCTCAAATAAATAAATAAATAAAAT1370.12628590391209762No Hit
TTTATAGGGGTTTCGGTGGGAATGACAACTTCGGTCGTGGAGGAAACTTCAG1370.12628590391209762No Hit
CTACATGGGCTGACAAGGGTCTTCTTGCTGCATCATCCCATGGCTGAAGTGG1370.12628590391209762No Hit
CGAATAGGGACGCATTTTGTTTTGCAAAATGAGAAGAGCTTATAAATACTTC1350.12444231407396482No Hit
AGCTTGGGGGAGGTATCACAGGTACATTGGAAAGAACATGAAATTTGAAATA1350.12444231407396482No Hit
GCTATAGGGACTGATTCCAATTTATTGCCTGTACCACATTATTCTTATTTTT1350.12444231407396482No Hit
GTTTATGGGACCGTAGGGCTCTGGAAGGGATTAATAAAATAATATGAGTAAA1340.12352051915489842No Hit
ATAGCGGGGGTTCTGGTTAGTGTAGAATTGTTCCAAAAGAAAATAGGTCAGC1340.12352051915489842No Hit
TCTGGAGGGATAAGTTAGGGAGAAAAAGTAAATGTTTCCATTTTGCACAAAA1340.12352051915489842No Hit
TATCAAGGGAGTCAACACGGAGCAGAGAGGGTGTATCATTAAAAGACCACAC1340.12352051915489842No Hit
CGTTGCGGGTACCTCTGGTAGAATTCGGCTGTGAATCCATCTGGTCCTGGAC1330.122598724235832No Hit
ATCTTTGGGGATTTGATTTCTTAGTCAGATGCTGTGGTACTTGGCATTTAAC1320.1216769293167656No Hit
GCTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1320.1216769293167656No Hit
GCTTTTGGGGGAGATAATTAGTCAAAGGGCACATGGAGTATGGAAGGGGGCA1310.1207551343976992No Hit
TGTTAGGGGATAATGACTTATGAATTAGCACAGTTAAGTTGACACTAGAAAC1310.1207551343976992No Hit
ATACACGGGCATTGCAGGATTTTAAGCTTTAATATATTTGCTGTTTAGACAC1310.1207551343976992No Hit
TACGAGGGAACCCAATCTCTAGAGTTGCTAAGCTTCTAAAAGCCTTAAATGT1310.1207551343976992No Hit
GCTGCTGGGGTGGGATTGCTGGGTTGAATGGTGGTTCTGTTTTGGTTCTTTG1300.1198333394786328No Hit
TTTGTTGGGTCTTCGGGTAAAGATGGCGGAGCGCGGGTACAGCTTTTCGCTG1300.1198333394786328No Hit
CGTTGGGGGTGACAAAAAAAATTAAGTCTTCAAATTTTTTAAAGGTTTTTTT1300.1198333394786328No Hit
GAAGCTGGGATAACATTAGAATGAGTAGCTGTAGTTGTGTGGACATTTATAA1300.1198333394786328No Hit
AACAAAGGGTGATATCATGGCCTCTAACCTGAACTCAGCAGTTCAAGTAGTT1300.1198333394786328No Hit
GAACATGGGAGGAAGAACCAAGGTACTTTTTTTGTAGGCAAAGGAAAAGTAT1300.1198333394786328No Hit
TGCTACCGGGGTCTCTAGGTGGAAAACAGCCTCAGGCCAAATAGGGAAGAAG1290.11891154455956637No Hit
TGAAGTGGGTGTCTCTTTCCGAGAGTAATGATGAAAACATTGAAGAAACCAT1290.11891154455956637No Hit
TCATAAGGGCCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGG1280.11798974964049999No Hit
TATTAAGGGTGTTTGTGTGTCCTGGATTATCAGAGAAGCAGTACTTGAAAAT1280.11798974964049999No Hit
ACATCAGGGGACCTATGGTGGTGACCAATGCATTGGTTACAGAAACTTTAAG1280.11798974964049999No Hit
GTTCTAGGGGACACAGAGAGAGACATTGAGATGAAACGGCAACTACGGCGAC1280.11798974964049999No Hit
TAAATTGGGTACTTAAAGGAACTGATTTTTTAAAATTGGATTGAATCATGGA1270.11706795472143357No Hit
ACGCGGGTAATGTAGAAAGCCACTAAAAAGTCTATTTGAAATTGCTCTTCTT1270.11706795472143357No Hit
TGCGGGGGAAATGTTTCAGGTCCTTCTCATCCGTCAAAGGCTGCAGCCACTA1260.11614615980236717No Hit
TGTTAAGGGTCACTTCTGTAATTACAATTTGTGAGAAATAACAGGGAACAAA1250.11522436488330076No Hit
GTGACTGGGACCTACGGAAAGTCTAAAGTATTCTGGATTGTCTTTAATATTG1250.11522436488330076No Hit
AGCAACGGGATTCCTATCTGATGATTTTAAATGGAACACTTAGAGAAGTAGG1240.11430256996423437No Hit
CCAGCAGGAGCTATTCTCTAGTATTATAAACTGTCATGGTGTGGAACAGATA1240.11430256996423437No Hit
CTCTCGCGGGACCCTTTGCCTGCCCCGAGTGTGGCCAGCGCTTCAGCCAGCG1240.11430256996423437No Hit
AAATGCGGGACAGGGTTTCAACATCAAAAGTGTACAATCACAAGGTTTTAAA1240.11430256996423437No Hit
GAGTGGGACTTGTCTTTGTGATGTTATACATGGAAGTGGAAAAATGTATTTC1230.11338077504516794No Hit
GATAATGGGACTGGTAGTATATGCTGTTTTCTGCTGTCTTTATCCCAAGTTT1230.11338077504516794No Hit
GCTAATGGGGATGGAAGCTTTCTCTTGGTAACAAAAATGGAGGGTGTATTAT1220.11245898012610155No Hit
GCTGCCGGGGATCTTAGAATGGGATACTAAAAACATGACTTGTTAGTTGTAT1220.11245898012610155No Hit
TTATAGGGGGACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGA1220.11245898012610155No Hit
TTGTTTGGGTGTTGTTTAGTGTTATATTCCAGACGTGATACCAAAATTTTGT1220.11245898012610155No Hit
GGAGTGGGGCTACTCCCCTTGGGTGTCACCTCCTTGAGTGAAGTACCTGCTG1220.11245898012610155No Hit
GTGTAAGGGATGGTTGACTCAATGTATAATTAACAAATATACATAGCAGAGT1220.11245898012610155No Hit
GGTGATGGGGTTCTAATAGAAGACTGTATCCCGGTACTGAAGAGGTACGCCA1210.11153718520703514No Hit
GTGTCGGGGGGGAGTAAAAGTATCCAAATAACAACAACAAAAAAAGCATCTG1210.11153718520703514No Hit
GCATTTGGGCATGCCCGCCCGCATAGGACTGATGCTTCATCTGGACTTGAAA1210.11153718520703514No Hit
GAATAAGGGCACTCTTAATTAACGAGGTGTTTGGTCGGAAAGGCATGTGATT1210.11153718520703514No Hit
AGTTGAGGGATTGTATAAATAATATGCTTTGAGTGGCAGGAAATAAAACTGA1210.11153718520703514No Hit
GACACTGGGTACCAGGACATAATTTTTGTCTACGCACTTCTCTCTCATTGAC1200.11061539028796874No Hit
TGACAAGGGGAAATTTGCATGTGACCTCATCTAGCTATGAATTCTGGGAAGT1200.11061539028796874No Hit
TGTTAGGGAGCGACACCACAGGCAAGCTGGGCTTTGAGGAATTCAAGTACTT1200.11061539028796874No Hit
GCACAAAGGGACCCTGAGCCATACGGAAACAACTTGAACTGTATATGGAAGA1200.11061539028796874No Hit
AGCCGAGGGAGTGTGAAAATTGCATTTTTCTTTATTTTATATGCCTGCATTT1200.11061539028796874No Hit
TTTGACGGGAGTGCTGCAGAACTTCGCCATGTGATGACAAACCTTGGAGAGA1200.11061539028796874No Hit
ATATTTGGGATGAACACAAAGGAGCTTTTAAGAGATGTCCATACCCTCCTTT1200.11061539028796874No Hit
GTGACGGGGGACTCAACTGCCCAGACACCTCATTGTACTTGAAAACTGGAAC1190.10969359536890232No Hit
GGCCAAAGGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCC1180.10877180044983592No Hit
TGATTGGGGTCTCAAGGTGGGTAGCTGTTTTACAATTAATTATCCAGTTACT1180.10877180044983592No Hit
ATATGAGGGAAGGCCCAATGTTATGGCCTAATGAAAGCTATATACTTGTGCT1180.10877180044983592No Hit
CTCACAGGGGAGCCCGATTAAAAACAGTGAAATTCAATTCTAAAACGAGAGA1180.10877180044983592No Hit
TCATCTGGGGTACTTGTGGAAACTAATCCCTAAATATGAATAGGTTTATATT1180.10877180044983592No Hit
GTTGCAGGGGAAGTCCAGGATTCTTCTGGCTTCCTGGTTTCCATTTCAATAT1180.10877180044983592No Hit
TGTTTGGGGACTTCTGCTTCCAGTGAAGCTGGCATAACAGAGACCAGGTTTA1180.10877180044983592No Hit
ATCAGCGGGCACTCTAAGAGGAAAACAGAGTAAATCTTTGTGACTTTGGATT1170.10785000553076951No Hit
CGCAGGGGGCAAGGAGTGCAAGCTTACATAAATTGTAAATATGATGCATCTA1170.10785000553076951No Hit
GCTGGAGGGAACTTAGGTAAGGTAAGGTAAAATAACCCTTTAATGTAGTCAT1170.10785000553076951No Hit
TAGGTGGGGATATTTAACTTTAAAGATTCAAGGAAATGATATTGTAAATAAT1170.10785000553076951No Hit
TTTTTAGGGATAAACTCAAGAGAATAATTTTTATAAACCCTGTTTTCCAATC1160.10692821061170311No Hit
GGCACGGGCGCTCTTCCGATCTAGCAAGGGGTTTGACAGACTGCAACCCTGC1160.10692821061170311No Hit
CGCGGTGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC1160.10692821061170311No Hit
GTTCTTGGGGCAATTATAACAGTGGAGTTCCCAAGCTTATTTCCTCTCCAAA1160.10692821061170311No Hit
AAGCGTGGGGCAAATGATTTCATGTGTGATGAGATGGAGCGCTCTTTACATG1150.10600641569263669No Hit
TAACAGCGGGAAGATGGTTTTTATTATTTTTTATACACTGTGTGCTTTTACT1150.10600641569263669No Hit
CTTGTTGGGATGTTTTCAAAGCAAACAATAGAATTCAAGAAAATGCTTAAAA1150.10600641569263669No Hit
AATCAGGGGGGCAGGGGCTGCTGGAGGAGCGTGGCCGCATCCTGCTGAGTCT1150.10600641569263669No Hit
AAGCTAGGGGGCCGTCGGCTCCGCGGCGCCGCCATGGCCGACGTGGAAGACG1150.10600641569263669No Hit
CACGTTGGGTAATGAGGCCTTATTGTCAACCCTCATAAATTCAGAGTATTTC1150.10600641569263669No Hit
GAATGGGTCCTAATCAAAATTCATATTTGCCTAGTCACTGAGGCAAAGAACT1150.10600641569263669No Hit
TAGCTTGGGATGTGGGAATTGTGGGAACTACAATTCAAGATGATATTTGGGT1150.10600641569263669No Hit
CAGCTAGGGAGAGGGACATTTACAGCACTAAATGCTTACAAGAGAAAGCAGG1140.10508462077357031No Hit
CAAGGGGACCTAGAAGAGATGGTCAACTCCTTGCTCAACACAGCGCAGCAGC1140.10508462077357031No Hit
GCTCTCGGGCATTTTTTAAATTGAGGTAAAATTCACATAACATAAAAGTAAC1140.10508462077357031No Hit
CTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1140.10508462077357031No Hit
GTATGCGGGTATTCCATTGAGATATATACATGTTGTTTATTCATCAGCTGAA1140.10508462077357031No Hit
CGAGTTGGGAAAAACCAAACTATTAAAATCCTCCTATAAATATTCTTTTTCT1140.10508462077357031No Hit
CATACTGGGAAGCATGAAAGAACGTGAGCACGGAGAAAAGGAGAGGCAGGTG1140.10508462077357031No Hit
TAAGCTGGGGAATAGAATTCGTTCAGCCGAACGAATGAGAATGGATGATTTT1140.10508462077357031No Hit
AGTTGCGGGGAATCTTCCAATTTTATCTATTTGATTCAAATGGTATGTTTTT1130.10416282585450388No Hit
GACACAGGGGCATGAAAAGGCAAAATGTGAGAGAAAGATCTTCTAGGCAGAA1130.10416282585450388No Hit
GTCCTGGGGATGACAAAGTGTAATTAACACTGATGTTTGTGTTAAATTTGCA1130.10416282585450388No Hit
GCTAACGGGGGAAACAGAAAGCAAACTGATAAAAAGCCAGTGAAGACAAAGA1130.10416282585450388No Hit
TTTGCCGGGGAGGTGGTATGGATCAACAAATGGACCCACAGATTTTTCTTAT1130.10416282585450388No Hit
AGCTACGGGATGCAAATAAGACAGTGGAGCTAAATTTTTTAATTAATGAAAG1130.10416282585450388No Hit
CTTGTCGGGAAGTTTTCTCTGTATCAAGTGCAGGAAAGGAACATGGGTAGAG1130.10416282585450388No Hit
CGAGTAGGGAGAAACCATTATGTAGCATGAAAGGCAAGCAGAAAGAGTAGTT1120.10324103093543749No Hit
GATTAAGGGAGCTAAAGAAATAAATTGGTAACAAAATAAAAATAAGAATGCA1120.10324103093543749No Hit
ACAACTGGGAGCCATTGGTTGCTGTTCAGGTCAGCTTTGCTCCTAACAACAC1120.10324103093543749No Hit
TGTCAAGGGAATCATAAAAAGGGATTAATCCAACCATTTTCTAGTAAAGCCA1120.10324103093543749No Hit
ACAAAGGGCCTAGATGTCTATTTATGGGATTACTTTTGCAGAATCATAATTT1120.10324103093543749No Hit
AGGCTCATCCCCAATACCACCATGCTATGCTATGAATAATAAAGTCCTTTGT1110.10231923601637108No Hit
TATTTTGGGATTCTGAGTCAGATCCTGGACTGAGAATCTGGGTCCTTTCTTT1110.10231923601637108No Hit
GATGAGAGGGAGAGTGCGGTTGTACATCCTCCCCACCTCATAACGCAGCCAC1110.10231923601637108No Hit
TAGCACGGGGACATCAGCAGTTGACACCTTCCCAGGAGCTGGATGATCTGAT1110.10231923601637108No Hit
TAAAAGGGGGCCCTCAGTGCAACACGATCACAGCGCCCGGAGACCTGCGGAG1110.10231923601637108No Hit
CAATCAGGGGCTTGATATAACTTCAAAGCCGATCTGGGCTGAGTCTTTTGGA1110.10231923601637108No Hit
TTTAAGGGGCTGTTTAATGGTCTTGGGCTTGGAGCAGTAATTGGCCTGGGAG1100.10139744109730468No Hit
ATGTTTGGGGGGCTGTTTTAACCATGAAAGGACCATAAGAAAGGCAATTCAC1100.10139744109730468No Hit
GTTCTAGGGAGAGGAGCAGTTGGAACCAGCAGTTGAAAAGATAAGCATCATG1100.10139744109730468No Hit
GCATTGGGGACCTATGTAAATAAAGCTGTTAATACAATGCTTTGACCCATAA1100.10139744109730468No Hit
TAGTGCGGGGACACATCAGACACTCCGTCCTCACACTGGCAGGACGGTGTTC1100.10139744109730468No Hit
TTCTAGGGGAATTTCGTGGTTCTCCATTTGGGGTAATTTTTCTCCCTTCCCT1100.10139744109730468No Hit
TGTCAGGGGGGACTGCACAGCCGAAGCAAGGAGAGCTCTCAAGTCACATCAA1100.10139744109730468No Hit
CACAATGGGAGTCTCACTGTGAATCCATTAAGTGACAGACTTTCCCTCCTAA1100.10139744109730468No Hit
TGTACTGGGGTCTCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCG1090.10047564617823826No Hit
CATCATGGGAGGAGTTCATAAGTATATTTAATGAAATTGGTGGTTTTAGGAA1090.10047564617823826No Hit
AATATGGGGCAACAGAGAAAGCTCCAGGAGTACCTAGAAGTCGAAAGGAACT1090.10047564617823826No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)