FastQCFastQC Report
Sat 31 Dec 2022
SRR5847488.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847488.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108768
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC7360.6766696087084437No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT5290.4863562812591939No Hit
CAGTTTGGGATATTCATTTAGCATTAAGTCAAGTAGACTGAGAATGACTCAA1860.17100617828773168No Hit
GAGCCTGGGAGAGGTCAGTGCTCACCAAACATCAGAGAATTCACACAAGGGT1670.15353780523683436No Hit
GTCCTGGGGATCGGACCTCGGCCAGTGTGAGCGGCAAGAGGATAGGAGATTA1640.15077964107090322No Hit
ATTAAAGGGCAACAAAGAGAGGTTCCAGAATTTTGGCCTAAGCAGAAAAATA1550.14250514857310972No Hit
GATTAAGGGAGCTAAAGAAATAAATTGGTAACAAAATAAAAATAAGAATGCA1510.1388275963518682No Hit
TTTGCCGGGGAGGTGGTATGGATCAACAAATGGACCCACAGATTTTTCTTAT1510.1388275963518682No Hit
AGCTTGGGGGAGGTATCACAGGTACATTGGAAAGAACATGAAATTTGAAATA1500.1379082082965578No Hit
TGCGGGGGAAATGTTTCAGGTCCTTCTCATCCGTCAAAGGCTGCAGCCACTA1490.13698882024124742No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAG1490.13698882024124742No Hit
CCTCCTAGGGGCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGG1470.13515004413062665No Hit
GATCTCGGGAAAGTCTTTTGGGGAAGACTGGCAATGTGCTTGAAGACCTTCA1460.13423065607531628No Hit
AGCCGAGGGAGTGTGAAAATTGCATTTTTCTTTATTTTATATGCCTGCATTT1460.13423065607531628No Hit
TAAATTGGGTACTTAAAGGAACTGATTTTTTAAAATTGGATTGAATCATGGA1450.1333112680200059No Hit
GAGTCTGGGAACTCGCAAAGTAGAATCCTTACCTACTACTCTTCTGATAATA1450.1333112680200059No Hit
TGCTACCGGGGTCTCTAGGTGGAAAACAGCCTCAGGCCAAATAGGGAAGAAG1450.1333112680200059No Hit
TGACTTGGGGGGCTAAGGACTTGTTCTATTATAGCACAGTAATCATTTTACT1450.1333112680200059No Hit
GTGCTCGGGCGATCTCGTCACGGGACAGTGAAAGAGTAAATTAGAAAGTGAA1440.1323918799646955No Hit
TTCAGAGGGGTGAAAATGGGATTGGGAAGCGGTATAAAGGAATTTGAAATTT1430.13147249190938512No Hit
TCGCGGGAGAAGAAATTAGAGAAGCATTCCGTGTGTTTGATAAGGATGGCAA1430.13147249190938512No Hit
ATTAAGGGGAGGTACTACCAAGGGGGGAAAATAGATAAATCTAATTGACCTA1420.13055310385407473No Hit
TACGAGGGAACCCAATCTCTAGAGTTGCTAAGCTTCTAAAAGCCTTAAATGT1410.12963371579876434No Hit
CTCACAGGGGAGCCCGATTAAAAACAGTGAAATTCAATTCTAAAACGAGAGA1410.12963371579876434No Hit
TGAAGTGGGTGTCTCTTTCCGAGAGTAATGATGAAAACATTGAAGAAACCAT1410.12963371579876434No Hit
TTGCAGGGGCCCGATAGTAAAGAATTTTCTGGCTCAAAATGTTGACAGTATT1400.12871432774345395No Hit
TAGATGGGGAGACATGGCTTTGAAAATAATTAAATGCTTTGCTAATAGACAA1390.12779493968814357No Hit
TATTAAGGGTGTTTGTGTGTCCTGGATTATCAGAGAAGCAGTACTTGAAAAT1390.12779493968814357No Hit
ACACCAGGGTTACAGGCCTTGACGTGTTAATGATTTCTGGCTTTCCTGATGG1380.1268755516328332No Hit
GCCGCAGGGAGATTCCTGCCAACTCAAAATACAGTACAGCTAGTAAAATGTT1380.1268755516328332No Hit
GGCCAAAGGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCC1370.1259561635775228No Hit
GATAATGGGACTGGTAGTATATGCTGTTTTCTGCTGTCTTTATCCCAAGTTT1370.1259561635775228No Hit
GCTCTCGGGCATTTTTTAAATTGAGGTAAAATTCACATAACATAAAAGTAAC1360.12503677552221243No Hit
GTTTCGGGGCAAACATATTGGTACGCATAGTAAAGTGCATCCTATTGCCAGT1360.12503677552221243No Hit
GACTGGGTAGATAAATCCTGCATGCCAACATTGTCTCTCAATTGAATGGGAG1350.12411738746690203No Hit
ATCTTTGGGGATTTGATTTCTTAGTCAGATGCTGTGGTACTTGGCATTTAAC1340.12319799941159164No Hit
CTACATGGGCTGACAAGGGTCTTCTTGCTGCATCATCCCATGGCTGAAGTGG1340.12319799941159164No Hit
CTACTCTGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAA1320.12135922330097086No Hit
ATAGCGGGGGTTCTGGTTAGTGTAGAATTGTTCCAAAAGAAAATAGGTCAGC1320.12135922330097086No Hit
TATGCTGATGATGTAGAAGAGTATATGATTGAACGACCAGAGCCAGAATTCC1320.12135922330097086No Hit
CGTTGGGGGTGACAAAAAAAATTAAGTCTTCAAATTTTTTAAAGGTTTTTTT1310.12043983524566049No Hit
CTTGTCGGGAAGTTTTCTCTGTATCAAGTGCAGGAAAGGAACATGGGTAGAG1310.12043983524566049No Hit
CGAGTAGGGAGAAACCATTATGTAGCATGAAAGGCAAGCAGAAAGAGTAGTT1300.1195204471903501No Hit
GATGAGAGGGAGAGTGCGGTTGTACATCCTCCCCACCTCATAACGCAGCCAC1300.1195204471903501No Hit
GTGACTGGGACCTACGGAAAGTCTAAAGTATTCTGGATTGTCTTTAATATTG1300.1195204471903501No Hit
TCGAGGGTGGAAGTTTACAGCTATTACCAGGGGGCTAACTCCCGGGGCATCG1300.1195204471903501No Hit
GCTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1290.11860105913503972No Hit
TGTTTGGGGACTTCTGCTTCCAGTGAAGCTGGCATAACAGAGACCAGGTTTA1290.11860105913503972No Hit
ACGCGGGTAATGTAGAAAGCCACTAAAAAGTCTATTTGAAATTGCTCTTCTT1280.11768167107972932No Hit
TATCAAGGGAGTCAACACGGAGCAGAGAGGGTGTATCATTAAAAGACCACAC1280.11768167107972932No Hit
TGTTAGGGAGCGACACCACAGGCAAGCTGGGCTTTGAGGAATTCAAGTACTT1270.11676228302441895No Hit
GTTAATGGGACCGTGCCTGGCCTAAACCTTACGCTTTTGAGGTTGAGTGCAG1260.11584289496910855No Hit
AGTTGAGGGATTGTATAAATAATATGCTTTGAGTGGCAGGAAATAAAACTGA1260.11584289496910855No Hit
CATACTGGGAAGCATGAAAGAACGTGAGCACGGAGAAAAGGAGAGGCAGGTG1260.11584289496910855No Hit
GCACAAAGGGACCCTGAGCCATACGGAAACAACTTGAACTGTATATGGAAGA1240.11400411885848778No Hit
ACACGAGGGTAGTGGTGCCATTGAATGATCTTCCAACACAGAGGCGATCACC1240.11400411885848778No Hit
GCTATAGGGACTGATTCCAATTTATTGCCTGTACCACATTATTCTTATTTTT1240.11400411885848778No Hit
ATACACGGGCATTGCAGGATTTTAAGCTTTAATATATTTGCTGTTTAGACAC1230.11308473080317741No Hit
AGGAAAGAAATCAGTGTAATAATGCTGAAGTATTTAATAGCAAAGGGTTATG1230.11308473080317741No Hit
AGTGTGGGGAGGCGAGTCATACTCTTGCAAGTAGTTAGCAAAGGGAGGCCCA1230.11308473080317741No Hit
TATGCAGGGGGAAAACAGCATGGATGTTCCTAAAAAAATTAAAAATAGAACC1230.11308473080317741No Hit
GTTCTAGGGGACACAGAGAGAGACATTGAGATGAAACGGCAACTACGGCGAC1230.11308473080317741No Hit
GCCTAAGGGCATTAAAGATAACCCAGACAGAATTTTCACTCACATTAGGATT1220.112165342747867No Hit
TGTTAAGGGTCACTTCTGTAATTACAATTTGTGAGAAATAACAGGGAACAAA1220.112165342747867No Hit
ACAACTGGGAGCCATTGGTTGCTGTTCAGGTCAGCTTTGCTCCTAACAACAC1220.112165342747867No Hit
ATTAATGGGTATGATGATATGATGATGATGGATGAATTTTATATATGAAAAA1220.112165342747867No Hit
TGTCACGGGGGTATCACAGCGTGCCACACAGGGATTTACGGTGGTCAAGAAG1220.112165342747867No Hit
TCACAGGGTAAGATACATCTATTCCTTTTTTTCCAGGGAATGTTATAAAATA1220.112165342747867No Hit
TTTCGCGGGCAAGCTCTCCAGATGATCTGAGGCAGGTATCTGAAGGGCTGTT1220.112165342747867No Hit
GTTTATGGGACCGTAGGGCTCTGGAAGGGATTAATAAAATAATATGAGTAAA1210.11124595469255663No Hit
GCACTGGGGCTGTACTGTCGCGTGCCATTGCTGAGCTGGGCATCTATCCAGC1210.11124595469255663No Hit
ACAGAAGGGCTAAATGACTTTTGACATACAAACTCTTCTTGAGAATGTTTGT1210.11124595469255663No Hit
TATTTTGGGATTCTGAGTCAGATCCTGGACTGAGAATCTGGGTCCTTTCTTT1200.11032656663724624No Hit
TTGGGTGGGCAGGAACCAGGCCACAGAAGAGCGTAGCATACTCTACTAAAAC1200.11032656663724624No Hit
TGACAAGGGGAAATTTGCATGTGACCTCATCTAGCTATGAATTCTGGGAAGT1200.11032656663724624No Hit
GCTAACGGGGGAAACAGAAAGCAAACTGATAAAAAGCCAGTGAAGACAAAGA1200.11032656663724624No Hit
AAATGGGGAAGACTAATATTTTACTTGATTTAGGAGGAATGCAGTTCAAAAT1200.11032656663724624No Hit
GTTATCGGGCCTCCACTCAGAGGCAGAGACTGAGTTAAGTCATAGGTGGCCT1200.11032656663724624No Hit
GTTCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1190.10940717858193587No Hit
CTTGTTGGGATGTTTTCAAAGCAAACAATAGAATTCAAGAAAATGCTTAAAA1190.10940717858193587No Hit
GGCACGGGCGCTCTTCCGATCTAGCAAGGGGTTTGACAGACTGCAACCCTGC1190.10940717858193587No Hit
TGAATAGGGAATAAGACAAAGTGTATATGGTAAGAGAAAAAAGCTCATCTGA1190.10940717858193587No Hit
AAAGATGGGGAGTGTTTTCCTGTACCATAGTAACTACATTGATTACTAAATC1190.10940717858193587No Hit
CTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1190.10940717858193587No Hit
TGTCAGGGGGGACTGCACAGCCGAAGCAAGGAGAGCTCTCAAGTCACATCAA1190.10940717858193587No Hit
AAATGCGGGACAGGGTTTCAACATCAAAAGTGTACAATCACAAGGTTTTAAA1190.10940717858193587No Hit
TAGCACGGGGACATCAGCAGTTGACACCTTCCCAGGAGCTGGATGATCTGAT1180.10848779052662547No Hit
TATCGACGGGAACCCAGCTCTTTTATCATGAGGAGAGGTAGCCATGCTGACT1180.10848779052662547No Hit
CAATCAGGGGCTTGATATAACTTCAAAGCCGATCTGGGCTGAGTCTTTTGGA1180.10848779052662547No Hit
TCTATGGGGCATGTGCCATATTATGCCTTTTCTTCAATGTGTGTCAGTTTTG1180.10848779052662547No Hit
TCTTGTGGGAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGA1170.10756840247131509No Hit
ACAAAGGGCCTAGATGTCTATTTATGGGATTACTTTTGCAGAATCATAATTT1170.10756840247131509No Hit
TCTGGAGGGATAAGTTAGGGAGAAAAAGTAAATGTTTCCATTTTGCACAAAA1160.1066490144160047No Hit
TGATCAGGGAGTGAAAGTGAATTACTTATACAAGATTCTAGTTTTACCTAAA1160.1066490144160047No Hit
GCATTGGGGACCTATGTAAATAAAGCTGTTAATACAATGCTTTGACCCATAA1160.1066490144160047No Hit
GTGCGTGGGAAAAAAGGGATGAGGTAAGTTAGTATGGAATGAACATTTCTTG1160.1066490144160047No Hit
AGCCATGGGGATCTTGGCTCACTGCAAGCTCTGCCTCCCAGGTTCACGCCAT1150.10572962636069433No Hit
AAATTAGGGAGGAACTGTGTGTGTCTTAAGACCGAAGTTCAATATGGTATTT1150.10572962636069433No Hit
GTTGCAGGGGAAGTCCAGGATTCTTCTGGCTTCCTGGTTTCCATTTCAATAT1150.10572962636069433No Hit
GTGTAAGGGATGGTTGACTCAATGTATAATTAACAAATATACATAGCAGAGT1150.10572962636069433No Hit
ATCGTTGGGAGGAAAAGGAATAGATTTCAGAGCTTATTTAGGAGATAAATTA1140.10481023830538393No Hit
ATCAGCGGGCACTCTAAGAGGAAAACAGAGTAAATCTTTGTGACTTTGGATT1140.10481023830538393No Hit
TTGTTTGGGTGTTGTTTAGTGTTATATTCCAGACGTGATACCAAAATTTTGT1140.10481023830538393No Hit
TAGCCTGGGCAGCAGAACGAGACTCCGTCTCAAATAAATAAATAAATAAAAT1140.10481023830538393No Hit
GTTCCAGGGAACTTTGTAATATTTTGGTTAGAAATATTTCAGTTAACTCCAG1140.10481023830538393No Hit
AAGCTAGGGGGGTTTTAAGGGGTGTGGCAGGAGGTTTTGGACTCGATGAGTT1140.10481023830538393No Hit
AATTTTGGGGATTACTTTTAATATGAAATAATTTTAAAAGGAGGCTTTTTAT1130.10389085025007355No Hit
GTAGTGGGGATGGGCTTTGGTGGCGCCTCTTTCTTGGTTTCCTGCTGCCCTT1130.10389085025007355No Hit
AAATCAGGGGATCTGATCGCGGGAAACTCCAGCATCACGCAATTTTTGCTTT1130.10389085025007355No Hit
GCTTTTGGGGGAGATAATTAGTCAAAGGGCACATGGAGTATGGAAGGGGGCA1130.10389085025007355No Hit
GCTGCCGGGGATCTTAGAATGGGATACTAAAAACATGACTTGTTAGTTGTAT1130.10389085025007355No Hit
CGAGTTGGGAAAAACCAAACTATTAAAATCCTCCTATAAATATTCTTTTTCT1130.10389085025007355No Hit
GACCATGGGAGGAATGAGAATGGTCAAAACATTCAATCGAGTATAAGAATCT1130.10389085025007355No Hit
CAAGGGGACCTAGAAGAGATGGTCAACTCCTTGCTCAACACAGCGCAGCAGC1120.10297146219476316No Hit
GTGACGGGGGACTCAACTGCCCAGACACCTCATTGTACTTGAAAACTGGAAC1120.10297146219476316No Hit
GAGCCGGGGTCAAGAAACTGCAGCAGAAAGTGGCCACCCTGGAGAAGCGCAA1120.10297146219476316No Hit
TGATTGGGGTCTCAAGGTGGGTAGCTGTTTTACAATTAATTATCCAGTTACT1120.10297146219476316No Hit
TATGATGGGCTTCCGATCTAGCAGGGATATGCAACCAACTAAACAGTTATAA1120.10297146219476316No Hit
GAAGCTGGGATAACATTAGAATGAGTAGCTGTAGTTGTGTGGACATTTATAA1120.10297146219476316No Hit
AGCTACGGGATGCAAATAAGACAGTGGAGCTAAATTTTTTAATTAATGAAAG1120.10297146219476316No Hit
ACTACACGGGTTGGATTGTTCCCTGGAGCCCCTCATTGATCCTTTGAACTTA1110.10205207413945278No Hit
AGGCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1110.10205207413945278No Hit
AAGCTAGGGGGCCGTCGGCTCCGCGGCGCCGCCATGGCCGACGTGGAAGACG1110.10205207413945278No Hit
ATATGTGGGAACAAACAGCTGTAGAATGTAATGACCCATCCTATTTCTAAAT1100.10113268608414239No Hit
GATTCTGGGGAAATAGAAAACCTATTTTAGAACTACAGAAATTTCACCCAGG1100.10113268608414239No Hit
TCATCTGGGGTACTTGTGGAAACTAATCCCTAAATATGAATAGGTTTATATT1100.10113268608414239No Hit
TACTTTGGGATTTAAAAGAAATGTTCTCACCTTGGGTTGATTGTGGTATACC1100.10113268608414239No Hit
GCTGGAGGGAACTTAGGTAAGGTAAGGTAAAATAACCCTTTAATGTAGTCAT1100.10113268608414239No Hit
TTCGCCGGGAGCCCTTCTTTGGGAATATAATTATCACCGACGTCACCGCGAA1090.10021329802883201No Hit
GTTCTAGGGAGAGGAGCAGTTGGAACCAGCAGTTGAAAAGATAAGCATCATG1090.10021329802883201No Hit
TTATAGGGGGACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGA1090.10021329802883201No Hit
TTGCGAGGGCTGATAGGACTGCAAAGGAAATTTGAGGCTAAGAGGTATGCAG1090.10021329802883201No Hit
TCTTCTGGGTTTTCCACTATGGCTTCCAGCACTGTCCCGGTGAGCGCTGCTG1090.10021329802883201No Hit
CACGTTGGGTAATGAGGCCTTATTGTCAACCCTCATAAATTCAGAGTATTTC1090.10021329802883201No Hit
CTCTCGCGGGACCCTTTGCCTGCCCCGAGTGTGGCCAGCGCTTCAGCCAGCG1090.10021329802883201No Hit
CTAATGGGGTTAGAATGGCGATCATTAAAAAGTTGGGAAACAACAGATGTTG1090.10021329802883201No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)