FastQCFastQC Report
Sat 31 Dec 2022
SRR5847477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129070
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC5010.38816146277213914No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT3920.3037111644843883No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1740.13481056790888665No Hit
GTCACCGGGTAGATTTTACAGCTTTGGCTTTCATTTTGTTTAGCTAAAGTCA1560.12086464709072596No Hit
TACTGAGGGAGGTTTATAGATGGCTACCTTTTAGATACTTTTTATAACATCA1520.11776555357557913No Hit
GTAAACGGGCAAAAAGAAAAGAAAAGAAAGAAAAGAAACAAACAACCGTCAA1500.11621600681800573No Hit
GAACAGGGGATTTTGCACCATGGATGTAGCTATTGGTCTTATGTGGGGCCAA1480.11466646006043231No Hit
AGCCCAGGGATTAAGTGTTGAAATGACATTAGGTAACAGGTTTAGAGACAGT1460.11311691330285892No Hit
AGCCGGGGGTGAAATGATGAGATGAAGTGAAATGATGAAATGAAATGAAAGA1450.11234213992407222No Hit
TCAGTGGGGGATGAACATAAGTGCAAAAATCCTCAATGAAATACTACCAAAC1450.11234213992407222No Hit
TAGCGAGGGGAAAGTTAAGCAGAGAAATCATAGCCATGGAATAACATACATT1440.11156736654528551No Hit
TGCTAGGGGCTTTCAAATAAATGGCAGTTAACTGGCTTTACAGTAAACATTG1430.11079259316649881No Hit
GATTTAGGGACTAAAGAACATTGGTCTGGGCAAAGATTTCATGAACAAGATC1390.10769349965135198No Hit
GCTGTGGGGGCCAAGGTAAACTTTGAACTCCACTCATTTCTGTGGACTCAAT1350.10459440613620516No Hit
GGCGCGGGGAAGAACCTTTAGATATAAATGCATTTTAACTGTTCTGGAGAGT1340.10381963275741844No Hit
GCGCACGGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGGG1310.10149531262105833No Hit
ATTCATGGGACATTTAAATTTAGTTTAAAAATTTTTTAACTTTTGTGTCAAA1300.10072053924227165No Hit
GCCACTGGGGTAACCTTAAGTGTCAATGGGCTAAATGTACCAATTAAAAGAC1300.10072053924227165No Hit
TCGTCAGGGCGCTCTTCCGATCTTCAAAAGGGAAGGCTTACTTTTTAAATCC1300.10072053924227165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)