FastQCFastQC Report
Sat 31 Dec 2022
SRR5847476.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847476.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130880
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC4060.3102078239608802No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT4040.308679706601467No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1940.14822738386308068No Hit
GCTGTGGGGGCCAAGGTAAACTTTGAACTCCACTCATTTCTGTGGACTCAAT1500.11460880195599023No Hit
TCAGTGGGGGATGAACATAAGTGCAAAAATCCTCAATGAAATACTACCAAAC1450.1107885085574572No Hit
AGCCCAGGGATTAAGTGTTGAAATGACATTAGGTAACAGGTTTAGAGACAGT1450.1107885085574572No Hit
GTAAACGGGCAAAAAGAAAAGAAAAGAAAGAAAAGAAACAAACAACCGTCAA1420.10849633251833742No Hit
GTCACCGGGTAGATTTTACAGCTTTGGCTTTCATTTTGTTTAGCTAAAGTCA1390.1062041564792176No Hit
CGAATAGGGAATATTTCAGCTGTTGAAACAGAAGAAGTTGAGCCTGATGAAG1380.10544009779951101No Hit
TACTGAGGGAGGTTTATAGATGGCTACCTTTTAGATACTTTTTATAACATCA1350.1031479217603912No Hit
CAAAGGGGGATTGATTGAGAGTTATGATCTTCTTGTATTCTTTTTCATCAAA1330.10161980440097801No Hit
GAACAGGGGATTTTGCACCATGGATGTAGCTATTGGTCTTATGTGGGGCCAA1320.10085574572127139No Hit
TAGCGAGGGGAAAGTTAAGCAGAGAAATCATAGCCATGGAATAACATACATT1320.10085574572127139No Hit
ACTACTGGGACAAGAAAAAAAGAATGCAAATCACCAATATTAAGAATGGAAA1310.10009168704156479No Hit
AGCCTGGGTGACAGAGTGAGACTCCATCTCAAACAAAAACAAAAACCATTAG1310.10009168704156479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)