FastQCFastQC Report
Sat 31 Dec 2022
SRR5847460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178138
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC10140.5692216147032076No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT9300.5220671614141845No Hit
CACACCGGGATGGCTCATTTGTTTTGCAAGGTGAAGAGTTTTTGAAGTTTTT2760.15493606080679026No Hit
CAGCGGGATCTGTACTTTGTTTAACTGAAAAAAATGTAGAAATATATGTAAA2570.14427017256284452No Hit
GCGTTTGGGATATTTCTCCAAAGAAGATACAAAAATGGACAATAAGCACAGG2460.13809518463213913No Hit
CCGGCTGGGGCCGAGGGTGGGGCGCGGGCGTAGTGGCGCCGGGAGTCGCGGG2400.1347270093972089No Hit
TCAAGGGCTAAAATATGGGGGTTAACTTTATTTTGATGAACATTTGAGGTAA2400.1347270093972089No Hit
TGACAAGGGATGCGCAGGCGCCGTGTGGCACTCGGCGGTCGAAAGGGGAGTT2360.13248155924058877No Hit
TACGTCCGGGTAGCCGGGTGTGGTGGCACACACCTGTAATCCCAGCTACATG2310.1296747465448136No Hit
CACTGTGGGTACTTAAGTACAAGTGATATTTAAAAATGATTCCTGAGCCCTT2280.12799065892734846No Hit
TTCATGGGGATGACAATTTGGCCACTGGAAATAGCTACTCCTGGGATAATTT2250.12630657130988335No Hit
AATGCTGGGATGAAATTTTTGGTTCGACGGTGGCATCGAGTCACAGGTTCTG2250.12630657130988335No Hit
GCTATGGGGGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCT2230.12518384623157328No Hit
TGCATGGGGAAGAAAAAAAGAGAGAAGACCCAAGTAAATAAAATCAGAGATG2220.12462248369241823No Hit
AAGTTTGGGCTTGTAAAATGCTTCTAAAAACTAAATTTTTATAAATTTATTT2220.12462248369241823No Hit
ATCATGGGTTGTGATAATTTGCTTCAGCAGCAAATAGAAAACTAATGCAAAA2210.1240611211532632No Hit
ATGGATGGGAGGACAAACCCCAAACAGTTAAATGTTTACTTTAAAAGATATT2190.12293839607495313No Hit
GACTTGGGAGGTCAAATATCTAACCACTTACCAGAAGGATGAGGAAAAGTTG2190.12293839607495313No Hit
TGCTTAGGGTATCATCATCAGGCCTAGAATATCCTAAATAGTTTCTCAATAT2180.12237703353579808No Hit
GCCAATGGGATTGCCCTTTCTTGATAATATCTTTGGATGAACAGACATTTTA2150.12069294591833299No Hit
GGGTTAGGGATTTTTCAGTCGCTATGTATGGATATACCCTGAATTAGATATG2140.12013158337917794No Hit
TACGCTGGGGCTTCTGCAACAGGCGTGGGTCACGCTCTCGCTCGGTCTTTCT2140.12013158337917794No Hit
CTAGCTAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2100.1178861332225578No Hit
AATAGTGGGACTGTTAATTCTAGTCTTCATAAATGTCCATTTAATTTGACAG2080.11676340814424772No Hit
GCACACGGGCAGGAGCAAGCAGCCGTGGACCCCTAAGCCGAGCTGGTGGAAG2050.11507932052678262No Hit
CTTATAGGGTATGTTGCCAGAGGTGCTGTCACTTCCTTAAGCATTCCATAAC2040.11451795798762758No Hit
TAGCATGGGACTGGTTGGTGGTTGGGTTTCTTTGCCCTGCCATGCTTTAAGG2040.11451795798762758No Hit
AAAGTAGGGGAAAATAAAATAAAATAAAATAAATCACAGAAGGAATAAATGA2030.11395659544847253No Hit
GATTTGGGGGGTGCCAGTGCACTCCAGCCTGAGCAACAGCATGAGATCCCAT2020.1133952329093175No Hit
GTCTTTGGGATTGTTTCCAGTTTGAGGATATTACCAACAATGTTAGAGGAAT2020.1133952329093175No Hit
AATTAGGGGAACCAGAGGATTTGCCAGAAACTGTTCCTTACTTAAAAATTTT2010.11283387037016246No Hit
TTATAGGGGCTTCTTTTCCGACAAAACACCAAATGGCGGATGACGCCGGTGC2010.11283387037016246No Hit
AATTTAGGGACAATAAGATTACAATTAGACTGGGGAGAGCACTTAAAAAAGG2010.11283387037016246No Hit
AAGGTTGGGTGGAAACCAAACTGAATATTGCTATAAATTTTGTTAAAATGTA2000.11227250783100741No Hit
GTTAGGGGGATCGCCATTGTCTGGCCATGGGGAGACTACTAGAATTAATGAG1990.11171114529185239No Hit
TTCTAAGGGTGTTTAGCAAAAAGCAGAATATTTCTAGCTCCCAGAAGCATGC1980.11114978275269735No Hit
ATCGGGGGAGAGAAATGACATCATTGTGTTTGAGACCATATGGTATCAGAAT1980.11114978275269735No Hit
ATGGATGGGGTGGTATATTTCACAGGAGAAGGGCATATTCAAAATGGCAAGG1970.11058842021354232No Hit
TTCAGCGGGGAAGTCGGTTTGTACTGATTTGTTTTACTATGTTTTTAAGCTT1970.11058842021354232No Hit
TCAAGAGGGATTACTGGGCAAATTGGCAAGATAATTATAGAATTGTCATTAA1970.11058842021354232No Hit
ATCAATGGGACAGTTGGTCAGTTCTACTTCTTGATCCGGAAGCGAATTCATC1970.11058842021354232No Hit
CACGGGGAATGTTAGTCACTTCCAAATGGCCTGTGTTTGATCTCTTTCCTTT1950.10946569513523223No Hit
GCACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1950.10946569513523223No Hit
CTTAAGGGGACTGTTCATCCAGTAAGCCAAGCCAGAAACCTAGGAGTCTTCT1940.10890433259607721No Hit
ATGCTTGGGAATGATGTGGCACCTCAAAATGAGATTTTCCCAAGGTTTGTGG1930.10834297005692217No Hit
GTCGACGGGAAGAGAACTGAAAACATATGTCTACACAAAAACTTGTACACTA1930.10834297005692217No Hit
GTATCAGGGTCTCACAAGGGGATTTGTACGAATTGGAGCAATGTTGGCTTAT1930.10834297005692217No Hit
AGGCGGGGAGGCTGGGAAATACCATTTAACTATCAGTTTAAGATTGAGATTC1930.10834297005692217No Hit
AAGGTAGGGAGGATAAAACCTGAGATATACTTGGAGGAAAATGATTGAATTT1930.10834297005692217No Hit
ATAGTAGGAACCTGAAATTAATCCATCTGTTTGATGACTTTGTCAATCAGAT1920.10778160751776712No Hit
ATCACTGGGGATGAACCTCAGCTATGTAAACCCGAACCACCAATCTAGAAGA1920.10778160751776712No Hit
CTCGTGGGGAGGTTCTCTTTGAGTCAATAGCTTGTCTTCGTCCATCTGTTGA1910.10722024497861209No Hit
ATCTCAGGGGAGCTGCTGAGTGTCTCTGGCTGGAACAATAGTATTTTTTTCT1910.10722024497861209No Hit
ATGTTAGGGGAGAAAAGAATATTAAAGTGTCATGGACATTATAAAATTTGGT1910.10722024497861209No Hit
TCAGTGGGGATGAGATTATTCCTTCAACACAACATATTATTGATTCAACATA1910.10722024497861209No Hit
TTCGGCGGGTAGCAAAAATGGTTGATGGTGATGCTTGACTCTTATAGATGCG1900.10665888243945705No Hit
AATAGTGGGAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTTT1900.10665888243945705No Hit
TTATGAAGGTGGCAGGCACTTCAAACCCTGGTGAGGAATTACCATTTTAAGC1890.106097519900302No Hit
TGCAGAGGGATGGGGATTCTGAGTCCATGCTGAGTGGGGTTTCTTTTCAGGA1890.106097519900302No Hit
GCATGCGGGAGGCTAAATGTTCCAATTAAAAGGCACAGAGCGGCAAGTTGGA1890.106097519900302No Hit
TTTTGCAGGGTGGAAGCAAGACCCAGATACCAACACAACGATATGGCCGGAG1880.10553615736114698No Hit
TGTTTAGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1880.10553615736114698No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTG1870.10497479482199194No Hit
TTTCAAGGGGTCTTCTGTAACTTGCTTTTAGCCTTAGGAATTTATTTTAGAG1870.10497479482199194No Hit
GGCCTAGGGGATTTAAAAACAAAACAAAAATTATCTTTGTAGGTGAATAAGT1870.10497479482199194No Hit
GTATGGGGGAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATGA1870.10497479482199194No Hit
GATCACGGGCTTCTGCTTCCTGACAGCTTCTTTTTCAAAATAACATGCAAAA1870.10497479482199194No Hit
GTCTTAGGGGGAAGTGTGATTTTTGGAAATTCTTAAAAACCACATTTTGAAA1870.10497479482199194No Hit
TTTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1870.10497479482199194No Hit
GACTGCGGGATAATCAACTGTGTATTCTTTTATATAGAATATATACTTTTTT1860.10441343228283691No Hit
TGGTAGGGGGAACTTCCGGCTCTCACTGCTAGGGGCTTAAGCGGAGGGAGTC1850.10385206974368186No Hit
TCTATTGGGGGAAAATGGCGGTTTCAAGTCCCCGATTCCGTGCCCACTTCAC1850.10385206974368186No Hit
GATTATGGGCAAGTGAGTGTTGGGTGGGGCAGGGCAAATCACCAAGCAGAGC1850.10385206974368186No Hit
AGGAGTGGGATGGATAAGAATTCAGTCATAGTCTTGGAAAAAATGACAAGAA1850.10385206974368186No Hit
AATGTGGGTAAGCAAAATCTGTCTTTCAGGACAGGTGCAGTGGGTCGCACCT1850.10385206974368186No Hit
ACAACAGGGGGAGGTGTTCACCCGCTTCTGCACCGGCCTCACCCAGATTGAA1840.10329070720452682No Hit
GTATTTGGGATGTTAAACAGTGGCTGCAGGAAATAGATCGTTATGCCAGTGA1840.10329070720452682No Hit
TTCTATGGGAGAACTGAAAGAGATAGAGACACAAAAAACCCGTCAAAAAAAT1830.1027293446653718No Hit
TAACAAGGGGGTAACTATCTTTCCCAAACTACTGCTGTTACCAGCAAGTGAT1830.1027293446653718No Hit
CACTGCGGGAACGCTTTATAATTGGCATAGACATAAAGGATAAAAGGAAAAT1830.1027293446653718No Hit
CAATCTGGGAGCGCGGGGAAATGAAATCTAAGATACATGAAGAAATTCTAAG1830.1027293446653718No Hit
CAAATAGGGATCACTGTGGCTGCCATGGTGGATGGAGGCTGGACAGAACCTG1830.1027293446653718No Hit
ACGATGGGATCTAGCTGGTTTATTCATTTATCTGCAAATGGACATTTGGCTG1830.1027293446653718No Hit
GTGTGCGGGGTAATAAATAAGGCTCTGCTAGTAGAGCCTAGACACTGGGAAT1820.10216798212621676No Hit
AATGTTGGGATTTTGTTGAAGGTCATGTCCTCCAGGTTTCTGCACGTTGCTG1820.10216798212621676No Hit
GCGACTGGGATAAGGATAGGTGGGAGAGGTCCATTCTTTCATAAGTTTGGAA1820.10216798212621676No Hit
TCGTCCGGGTCCCCACGGCTTGTGAGCCCATGCTGGAGCGACCTTTTAAACT1820.10216798212621676No Hit
GTGCATGGGCAACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGC1810.10160661958706171No Hit
CAATAAAGGCTGATTTATTTGGACACAAAACAAATTTTTAACTCATAAATGC1810.10160661958706171No Hit
GTTTCTGGGAGAACTACAAAACGCATGTATGATAGTTACTGGCGGCAGTTCA1810.10160661958706171No Hit
GATCCGGGGGAATAATTGGTGTTCTTGAAGAAAAAGAGAAATCTAAAACTTT1800.10104525704790668No Hit
CGATAGGGGGTGAAAAATTAGGTCTAAACATACAGTAATAAGAAAATAGTTA1800.10104525704790668No Hit
GTTCATGGGGGAGGTCCAACTGCCGTAGACAGGAAGGAATTAAAATTGTCCT1790.10048389450875164No Hit
AAAATAGGGATACATTCCCATTACAGTAGTAGAACTTAAAAATACTAAACAC1790.10048389450875164No Hit
TTGTAGGGGGGAATATGGCGGGGCGTTTGGCTCTTGCTGCAGCCAGAGCTCC1790.10048389450875164No Hit
TATACAGGGATCTTTGAAGAGTCGTGTGGTGTGAATTGGTTTGTGTACATTA1790.10048389450875164No Hit
CTCACTGGGACTGTAAGATGGTCAAAAGCTGACAGCCTGTGTATGTGAAAAG1790.10048389450875164No Hit
GCAGTTGGGGTTCAGTGCTAATTTGCTTACTGTGTTACTGTATTGTTTTCCT1790.10048389450875164No Hit
TATTTAGGGGATCTGGTGGTGGGATAGTCTAGTTTTTGTATATTGGTTACTT1790.10048389450875164No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)