FastQCFastQC Report
Sat 31 Dec 2022
SRR5847459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177275
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC10300.5810181920744606No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT8480.4783528416302355No Hit
TGCTTAGGGTATCATCATCAGGCCTAGAATATCCTAAATAGTTTCTCAATAT2700.15230573967000424No Hit
CACACCGGGATGGCTCATTTGTTTTGCAAGGTGAAGAGTTTTTGAAGTTTTT2570.1449725003525596No Hit
TAACAAGGGGGTAACTATCTTTCCCAAACTACTGCTGTTACCAGCAAGTGAT2490.14045973769567058No Hit
TTCATGGGGATGACAATTTGGCCACTGGAAATAGCTACTCCTGGGATAATTT2400.13538287970667043No Hit
TACCACGGGAACTTGAAGACAGTGATTTAAAGTTGGAGTATCCATAAATCAT2340.13199830771400367No Hit
ATGGATGGGAGGACAAACCCCAAACAGTTAAATGTTTACTTTAAAAGATATT2320.1308701170497814No Hit
AATTTAGGGACAATAAGATTACAATTAGACTGGGGAGAGCACTTAAAAAAGG2280.1286137357213369No Hit
GCGTTTGGGATATTTCTCCAAAGAAGATACAAAAATGGACAATAAGCACAGG2270.12804964038922578No Hit
TTATAGGGGCTTCTTTTCCGACAAAACACCAAATGGCGGATGACGCCGGTGC2260.12748554505711465No Hit
TAGCATGGGACTGGTTGGTGGTTGGGTTTCTTTGCCCTGCCATGCTTTAAGG2260.12748554505711465No Hit
TACGTCCGGGTAGCCGGGTGTGGTGGCACACACCTGTAATCCCAGCTACATG2250.12692144972500352No Hit
TGCATGGGGAAGAAAAAAAGAGAGAAGACCCAAGTAAATAAAATCAGAGATG2240.12635735439289242No Hit
GTGCATGGGCAACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGC2240.12635735439289242No Hit
ATGCTTGGGAATGATGTGGCACCTCAAAATGAGATTTTCCCAAGGTTTGTGG2210.12466506839655901No Hit
AAGGTTGGGTGGAAACCAAACTGAATATTGCTATAAATTTTGTTAAAATGTA2210.12466506839655901No Hit
CTAGCTAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2200.1241009730644479No Hit
AATAGTGGGAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTTT2170.12240868706811452No Hit
GTCGACGGGAAGAGAACTGAAAACATATGTCTACACAAAAACTTGTACACTA2150.12128049640389225No Hit
AAGGTAGGGAGGATAAAACCTGAGATATACTTGGAGGAAAATGATTGAATTT2130.12015230573966999No Hit
TCAGTGGGGATGAGATTATTCCTTCAACACAACATATTATTGATTCAACATA2130.12015230573966999No Hit
TTCAGCGGGGAAGTCGGTTTGTACTGATTTGTTTTACTATGTTTTTAAGCTT2100.11846001974333661No Hit
CACAACGGGGCATCGTGAGCTGCAGCAGTTGCACTCTTTTTCAGTGACCTAA2090.1178959244112255No Hit
AGGCGGGGAGGCTGGGAAATACCATTTAACTATCAGTTTAAGATTGAGATTC2090.1178959244112255No Hit
GCATGCGGGAGGCTAAATGTTCCAATTAAAAGGCACAGAGCGGCAAGTTGGA2090.1178959244112255No Hit
AATGTTGGGATTTTGTTGAAGGTCATGTCCTCCAGGTTTCTGCACGTTGCTG2080.11733182907911438No Hit
TCAAGGGCTAAAATATGGGGGTTAACTTTATTTTGATGAACATTTGAGGTAA2080.11733182907911438No Hit
TACGCTGGGGCTTCTGCAACAGGCGTGGGTCACGCTCTCGCTCGGTCTTTCT2070.11676773374700324No Hit
GTTAGGGGGATCGCCATTGTCTGGCCATGGGGAGACTACTAGAATTAATGAG2060.11620363841489212No Hit
GCGACTGGGATAAGGATAGGTGGGAGAGGTCCATTCTTTCATAAGTTTGGAA2060.11620363841489212No Hit
CAATCTGGGAGCGCGGGGAAATGAAATCTAAGATACATGAAGAAATTCTAAG2060.11620363841489212No Hit
TGACAAGGGATGCGCAGGCGCCGTGTGGCACTCGGCGGTCGAAAGGGGAGTT2060.11620363841489212No Hit
TTTCAAGGGGTCTTCTGTAACTTGCTTTTAGCCTTAGGAATTTATTTTAGAG2050.115639543082781No Hit
TACAACGGGCTCCCAAAGTGCTGGGATTAAAGGCGTGAGCCACCACGTGCGG2050.115639543082781No Hit
CACTGTGGGTACTTAAGTACAAGTGATATTTAAAAATGATTCCTGAGCCCTT2050.115639543082781No Hit
ATCATGGGTTGTGATAATTTGCTTCAGCAGCAAATAGAAAACTAATGCAAAA2040.11507544775066986No Hit
ATCGGGGGAGAGAAATGACATCATTGTGTTTGAGACCATATGGTATCAGAAT2040.11507544775066986No Hit
CTCGTGGGGAGGTTCTCTTTGAGTCAATAGCTTGTCTTCGTCCATCTGTTGA2030.11451135241855874No Hit
GCACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2030.11451135241855874No Hit
GACTTGGGAGGTCAAATATCTAACCACTTACCAGAAGGATGAGGAAAAGTTG2030.11451135241855874No Hit
GTGCCGGGGGGAGATAGTGTAGTGCAAAGGATCATGGTTTGGAATTTGGAAG2010.11338316175433648No Hit
GCACTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2010.11338316175433648No Hit
GTCTTTGGGATTGTTTCCAGTTTGAGGATATTACCAACAATGTTAGAGGAAT2010.11338316175433648No Hit
TCAAGAGGGATTACTGGGCAAATTGGCAAGATAATTATAGAATTGTCATTAA2000.11281906642222536No Hit
ACAACAGGGGGAGGTGTTCACCCGCTTCTGCACCGGCCTCACCCAGATTGAA2000.11281906642222536No Hit
ACGATGGGATCTAGCTGGTTTATTCATTTATCTGCAAATGGACATTTGGCTG2000.11281906642222536No Hit
GATTTGGGGGGTGCCAGTGCACTCCAGCCTGAGCAACAGCATGAGATCCCAT1990.11225497109011423No Hit
TTGACAGGGAAGGAAGTAACTGCAAAACACTAGGCTTTAGTAGGTACTTATA1990.11225497109011423No Hit
GCTATGGGGGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCT1990.11225497109011423No Hit
CTTAGGGGCTTCATAATTGTCATTTTGGAATGAAACAGTTGAGCTATTTTTA1970.11112678042589198No Hit
ATGTTAGGGGACATTTTGGCAGGTTCCAAAAGATGAAACTGATTTAATGCGG1970.11112678042589198No Hit
AATAGTGGGACTGTTAATTCTAGTCTTCATAAATGTCCATTTAATTTGACAG1970.11112678042589198No Hit
GTATGGGGGAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATGA1960.11056268509378085No Hit
ATAGTAGGAACCTGAAATTAATCCATCTGTTTGATGACTTTGTCAATCAGAT1960.11056268509378085No Hit
GACCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1960.11056268509378085No Hit
CAGCGGGATCTGTACTTTGTTTAACTGAAAAAAATGTAGAAATATATGTAAA1960.11056268509378085No Hit
CTCACTGGGACTGTAAGATGGTCAAAAGCTGACAGCCTGTGTATGTGAAAAG1950.10999858976166972No Hit
CAATAGGGGATATGGAACCTGGCAAGACTGAATCATTTTACTGTGAAATATA1940.10943449442955859No Hit
TGCTCAGGGAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT1940.10943449442955859No Hit
TCTATTGGGGGAAAATGGCGGTTTCAAGTCCCCGATTCCGTGCCCACTTCAC1940.10943449442955859No Hit
GCCTCAGGGTGGACTAATAATGGCTGAGGTTTAATGGTAATACTGAATTTAA1940.10943449442955859No Hit
CTTATAGGGTATGTTGCCAGAGGTGCTGTCACTTCCTTAAGCATTCCATAAC1930.10887039909744747No Hit
TGCAGAGGGATGGGGATTCTGAGTCCATGCTGAGTGGGGTTTCTTTTCAGGA1930.10887039909744747No Hit
TACTGTGGGAGTGGAGGAGATGCCCATGAATGTGGCTGACCTTATTTAATTC1930.10887039909744747No Hit
GCTGCAGGGCAAAATAGAAGGGTCCTAAATCACGTTAACTCAAACATTGTAG1920.10830630376533634No Hit
TTAACTGGGGACGTGGGTGGGGCCGGATAAGAGAATAAAAGCAGTCTGCCCC1920.10830630376533634No Hit
CCGGCTGGGGCCGAGGGTGGGGCGCGGGCGTAGTGGCGCCGGGAGTCGCGGG1910.10774220843322521No Hit
GTATTTGGGATGTTAAACAGTGGCTGCAGGAAATAGATCGTTATGCCAGTGA1910.10774220843322521No Hit
CTACCAGGGGCAGGGGAAGACCAAAGTTTGCAACTCGAACTGCTTTCGTCCA1910.10774220843322521No Hit
GATGGTGGGTGAGTTTACCTGATTAAACTTTAAAAGTTAATTGTAGATTTAA1900.1071781131011141No Hit
ATGGATGGGGTGGTATATTTCACAGGAGAAGGGCATATTCAAAATGGCAAGG1900.1071781131011141No Hit
ATCAATGGGACAGTTGGTCAGTTCTACTTCTTGATCCGGAAGCGAATTCATC1900.1071781131011141No Hit
TTGTAGGGGGGAATATGGCGGGGCGTTTGGCTCTTGCTGCAGCCAGAGCTCC1890.10661401776900298No Hit
TTATGAAGGTGGCAGGCACTTCAAACCCTGGTGAGGAATTACCATTTTAAGC1880.10604992243689183No Hit
AATATTGGGTAATAACTGCTGGCAGCTTGATAAGAACAAGGTGCTTGCAGAG1880.10604992243689183No Hit
ATCCAAGGGCATGATACCCTGTGCTTCTGAGACAAGAACTCCAGTATTTACA1880.10604992243689183No Hit
TGTTTAGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1870.10548582710478072No Hit
AAGTTTGGGCTTGTAAAATGCTTCTAAAAACTAAATTTTTATAAATTTATTT1860.10492173177266957No Hit
GTCTTAGGGGGAAGTGTGATTTTTGGAAATTCTTAAAAACCACATTTTGAAA1860.10492173177266957No Hit
TTATCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1860.10492173177266957No Hit
GCAGTTGGGGTTCAGTGCTAATTTGCTTACTGTGTTACTGTATTGTTTTCCT1860.10492173177266957No Hit
GATTATGGGCAAGTGAGTGTTGGGTGGGGCAGGGCAAATCACCAAGCAGAGC1850.10435763644055845No Hit
CACACAGGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGCCACA1850.10435763644055845No Hit
CTGACGGGGCTCAATTCTTTTTTTTCCGCCCTTCCTTGGCAATGGCTGGATG1840.10379354110844734No Hit
ACCATGGGGGCAGGCCGTATTCGGACGCGCCAAGCTCGATGCGCGCGACATC1840.10379354110844734No Hit
CAATAAAGGCTGATTTATTTGGACACAAAACAAATTTTTAACTCATAAATGC1830.10322944577633619No Hit
TTTAGGGTAGCCGTGATCCTGCAGCATATCCTGACATGGAGTCTGATATACG1810.10210125511211396No Hit
CACTGTGGGCAGCCATGCCGTCCCGAGATGAATGAACACATCGGCCATGCTG1810.10210125511211396No Hit
CTGTCTGGGGAGACCATCCTGGGCAACATAGCAAGACCCCTTTATCTATAAA1810.10210125511211396No Hit
GACTGGGTGGGCTGGAGGGTGCAATTGAGATGGGCCAAATCTATACCAAGCT1810.10210125511211396No Hit
GGCTTTGGGACTCAAACTTTCTCCATATCAACAGTAAGACTTTTGTTTTCTT1800.10153715978000281No Hit
ACATTGGGGATTAAAACAGATCCAGATTTAAATGACCTTATTATCAAGTTTC1800.10153715978000281No Hit
CGATAGGGGGTGAAAAATTAGGTCTAAACATACAGTAATAAGAAAATAGTTA1800.10153715978000281No Hit
TGTTGAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC1790.1009730644478917No Hit
TTTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1790.1009730644478917No Hit
AGACCCCGACTCCCTTTGGCTCAGCCCGCGCGCCCCAGGCCCGGCCCGGGCG1790.1009730644478917No Hit
CCTTGGATTTACCTGGTCCATTTGGATCAAGTTCTTTCACCTATTCACATGA1790.1009730644478917No Hit
AATGTGGGTAAGCAAAATCTGTCTTTCAGGACAGGTGCAGTGGGTCGCACCT1790.1009730644478917No Hit
GGCCTAGGGGATTTAAAAACAAAACAAAAATTATCTTTGTAGGTGAATAAGT1780.10040896911578055No Hit
GTGTGCGGGGTAATAAATAAGGCTCTGCTAGTAGAGCCTAGACACTGGGAAT1780.10040896911578055No Hit
AGTGCGGGGACCAAAACAGAAATGTAATGTGTTGTATATACCAAACCATGCC1780.10040896911578055No Hit
CAAACAGGGAGAGAAAATCATCAATAAATATTTATTACATTCTGACAGGGTG1780.10040896911578055No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)