FastQCFastQC Report
Sat 31 Dec 2022
SRR5847458.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847458.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177193
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC10340.5835444966787627No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT9370.5288019278413933No Hit
CACACCGGGATGGCTCATTTGTTTTGCAAGGTGAAGAGTTTTTGAAGTTTTT2800.15801978633467462No Hit
TAGCATGGGACTGGTTGGTGGTTGGGTTTCTTTGCCCTGCCATGCTTTAAGG2550.14391087684050724No Hit
TGCTTAGGGTATCATCATCAGGCCTAGAATATCCTAAATAGTTTCTCAATAT2420.1365742439035402No Hit
AATAGTGGGACTGTTAATTCTAGTCTTCATAAATGTCCATTTAATTTGACAG2360.13318810562494005No Hit
ATCATGGGTTGTGATAATTTGCTTCAGCAGCAAATAGAAAACTAATGCAAAA2240.12641582906773968No Hit
TACGTCCGGGTAGCCGGGTGTGGTGGCACACACCTGTAATCCCAGCTACATG2220.1252871163082063No Hit
TACGCTGGGGCTTCTGCAACAGGCGTGGGTCACGCTCTCGCTCGGTCTTTCT2190.12359404716890622No Hit
CACTGTGGGTACTTAAGTACAAGTGATATTTAAAAATGATTCCTGAGCCCTT2180.12302969078913951No Hit
TTCATGGGGATGACAATTTGGCCACTGGAAATAGCTACTCCTGGGATAATTT2150.12133662164983944No Hit
TGACAAGGGATGCGCAGGCGCCGTGTGGCACTCGGCGGTCGAAAGGGGAGTT2140.12077226527007275No Hit
CTAGCTAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2140.12077226527007275No Hit
TACTGTGGGAGTGGAGGAGATGCCCATGAATGTGGCTGACCTTATTTAATTC2130.12020790889030605No Hit
AATGCTGGGATGAAATTTTTGGTTCGACGGTGGCATCGAGTCACAGGTTCTG2120.11964355251053936No Hit
ATGCTTGGGAATGATGTGGCACCTCAAAATGAGATTTTCCCAAGGTTTGTGG2110.11907919613077267No Hit
CAAAAAGGGCAGCTAGAACACCTTCCAGACACTTGCTGGACAGCTATCTTCC2110.11907919613077267No Hit
GCGTTTGGGATATTTCTCCAAAGAAGATACAAAAATGGACAATAAGCACAGG2110.11907919613077267No Hit
GGCTTTGGGACTCAAACTTTCTCCATATCAACAGTAAGACTTTTGTTTTCTT2110.11907919613077267No Hit
GTTAGGGGGATCGCCATTGTCTGGCCATGGGGAGACTACTAGAATTAATGAG2100.11851483975100596No Hit
GCGACTGGGATAAGGATAGGTGGGAGAGGTCCATTCTTTCATAAGTTTGGAA2100.11851483975100596No Hit
TCAAGGGCTAAAATATGGGGGTTAACTTTATTTTGATGAACATTTGAGGTAA2100.11851483975100596No Hit
CTTAGGGGCTTCATAATTGTCATTTTGGAATGAAACAGTTGAGCTATTTTTA2080.11738612699147258No Hit
AGGAGTGGGATGGATAAGAATTCAGTCATAGTCTTGGAAAAAATGACAAGAA2080.11738612699147258No Hit
CACACAGGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGCCACA2080.11738612699147258No Hit
TTCAGCGGGGAAGTCGGTTTGTACTGATTTGTTTTACTATGTTTTTAAGCTT2070.11682177061170587No Hit
AGGCGGGGAGGCTGGGAAATACCATTTAACTATCAGTTTAAGATTGAGATTC2070.11682177061170587No Hit
ACGATGGGATCTAGCTGGTTTATTCATTTATCTGCAAATGGACATTTGGCTG2060.1162574142319392No Hit
GCACTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2060.1162574142319392No Hit
TACCACGGGAACTTGAAGACAGTGATTTAAAGTTGGAGTATCCATAAATCAT2030.1145643450926391No Hit
GCATGCGGGAGGCTAAATGTTCCAATTAAAAGGCACAGAGCGGCAAGTTGGA2030.1145643450926391No Hit
TTGCGCGGGGAAGGGAGGAAAGAAGGATCACTGAGAGGACAGACATATGAGA2010.1134356323331057No Hit
GCACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2010.1134356323331057No Hit
AAGGTAGGGAGGATAAAACCTGAGATATACTTGGAGGAAAATGATTGAATTT2010.1134356323331057No Hit
AAAATAGGGATACATTCCCATTACAGTAGTAGAACTTAAAAATACTAAACAC2000.11287127595333903No Hit
AAGTTTGGGCTTGTAAAATGCTTCTAAAAACTAAATTTTTATAAATTTATTT2000.11287127595333903No Hit
ATGGATGGGAGGACAAACCCCAAACAGTTAAATGTTTACTTTAAAAGATATT2000.11287127595333903No Hit
AATAGTGGGAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTTT2000.11287127595333903No Hit
TTCTAAGGGTGTTTAGCAAAAAGCAGAATATTTCTAGCTCCCAGAAGCATGC1990.11230691957357232No Hit
TCAGTGGGGATGAGATTATTCCTTCAACACAACATATTATTGATTCAACATA1980.11174256319380561No Hit
TTTAGGGTAGCCGTGATCCTGCAGCATATCCTGACATGGAGTCTGATATACG1970.11117820681403894No Hit
CTGTCTGGGGAGACCATCCTGGGCAACATAGCAAGACCCCTTTATCTATAAA1970.11117820681403894No Hit
TTGACAGGGAAGGAAGTAACTGCAAAACACTAGGCTTTAGTAGGTACTTATA1970.11117820681403894No Hit
GTCGACGGGAAGAGAACTGAAAACATATGTCTACACAAAAACTTGTACACTA1970.11117820681403894No Hit
GACTGCGGGATAATCAACTGTGTATTCTTTTATATAGAATATATACTTTTTT1970.11117820681403894No Hit
GTATGGGGGAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATGA1960.11061385043427223No Hit
GCTCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1950.11004949405450552No Hit
TGCATGGGGAAGAAAAAAAGAGAGAAGACCCAAGTAAATAAAATCAGAGATG1940.10948513767473884No Hit
GATGGTGGGTGAGTTTACCTGATTAAACTTTAAAAGTTAATTGTAGATTTAA1940.10948513767473884No Hit
GTGCCGGGGGGAGATAGTGTAGTGCAAAGGATCATGGTTTGGAATTTGGAAG1940.10948513767473884No Hit
GCTATGGGGGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCT1940.10948513767473884No Hit
GTATCAGGGTCTCACAAGGGGATTTGTACGAATTGGAGCAATGTTGGCTTAT1940.10948513767473884No Hit
TTTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1930.10892078129497215No Hit
TAGCTGGGGGAATGGCATTTTTTAGAATAAAACTTCTTCCATTCTCATTTAC1930.10892078129497215No Hit
CACTGGGGGAGCTTGTCTGACATTTTTTGTGTCTAAAACATTCTGAATTTGG1930.10892078129497215No Hit
CAGCGGGATCTGTACTTTGTTTAACTGAAAAAAATGTAGAAATATATGTAAA1910.10779206853543877No Hit
CGACAGGGAAGAGTGGAAATTTATTAAAATCAGAAAGTATGAGATCCTGTTA1910.10779206853543877No Hit
TGTTGAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC1900.10722771215567206No Hit
GTGTCCGGGATGGTGGTGGAGGACCTGGATATGGCAACCAGGGTGGGGGCTA1890.10666335577590538No Hit
TTGTAGGGGGGAATATGGCGGGGCGTTTGGCTCTTGCTGCAGCCAGAGCTCC1890.10666335577590538No Hit
CACTGCGGGAACGCTTTATAATTGGCATAGACATAAAGGATAAAAGGAAAAT1880.10609899939613868No Hit
TTAACTGGGGACGTGGGTGGGGCCGGATAAGAGAATAAAAGCAGTCTGCCCC1880.10609899939613868No Hit
TCTATTGGGGGAAAATGGCGGTTTCAAGTCCCCGATTCCGTGCCCACTTCAC1880.10609899939613868No Hit
GACTTGGGAGGTCAAATATCTAACCACTTACCAGAAGGATGAGGAAAAGTTG1880.10609899939613868No Hit
ATCGGGGGAGAGAAATGACATCATTGTGTTTGAGACCATATGGTATCAGAAT1880.10609899939613868No Hit
CTACACAGGGGCAACAAATACCTGGGAATTAAAATAATGTGTGTCTGTTGAT1880.10609899939613868No Hit
GTCTTTGGGATTGTTTCCAGTTTGAGGATATTACCAACAATGTTAGAGGAAT1880.10609899939613868No Hit
CCGGCTGGGGCCGAGGGTGGGGCGCGGGCGTAGTGGCGCCGGGAGTCGCGGG1870.10553464301637197No Hit
ATCACTGGGGATGAACCTCAGCTATGTAAACCCGAACCACCAATCTAGAAGA1870.10553464301637197No Hit
TTCTATGGGAGAACTGAAAGAGATAGAGACACAAAAAACCCGTCAAAAAAAT1850.10440593025683859No Hit
GTGCATGGGCAACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGC1850.10440593025683859No Hit
GGTCTTGGGCCGATCTAATAAAGGGATTCAAGAATTTATACTTATTTTTTCC1850.10440593025683859No Hit
ATCAATGGGACAGTTGGTCAGTTCTACTTCTTGATCCGGAAGCGAATTCATC1850.10440593025683859No Hit
TTATTAGGGAGATAGATTGAAAGATAAACTTGAAAAGAATTTCTCAGAAAAG1850.10440593025683859No Hit
GGCCTAGGGGATTTAAAAACAAAACAAAAATTATCTTTGTAGGTGAATAAGT1840.10384157387707188No Hit
AATGTGGGTAAGCAAAATCTGTCTTTCAGGACAGGTGCAGTGGGTCGCACCT1840.10384157387707188No Hit
TAGGCTGGGACATAAGAACAAACTACAAAATATTTATGCAAAAATCACAGAA1830.1032772174973052No Hit
CTTACGGGTATTATCTAAATAAAACTCTCCTTGAGATCGATTGATAGTTTTT1830.1032772174973052No Hit
GTTTCTGGGAGAACTACAAAACGCATGTATGATAGTTACTGGCGGCAGTTCA1830.1032772174973052No Hit
AGACCCCGACTCCCTTTGGCTCAGCCCGCGCGCCCCAGGCCCGGCCCGGGCG1830.1032772174973052No Hit
TGACAAGGGGTGTGAACCTAGTTATAATGACTTAAAATTCATGGTCCCAAAC1830.1032772174973052No Hit
CTTATTGTGGGATGCCAACTACTAAGACAAAGCTTTAACAAAGTTTATAGAA1820.1027128611175385No Hit
CATGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1820.1027128611175385No Hit
GCAGCTGGGGAATAGGGGGTGTTTTAACCAATTTATGGTTTTGCATTTGATG1820.1027128611175385No Hit
AAAGTAGGGGAAAATAAAATAAAATAAAATAAATCACAGAAGGAATAAATGA1820.1027128611175385No Hit
TATTTAGGGGATCTGGTGGTGGGATAGTCTAGTTTTTGTATATTGGTTACTT1820.1027128611175385No Hit
TGCTCAGGGAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT1810.1021485047377718No Hit
GGCGTGGGGGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCAGAGCTGCGG1810.1021485047377718No Hit
TTATAGGGGCTTCTTTTCCGACAAAACACCAAATGGCGGATGACGCCGGTGC1800.10158414835800512No Hit
CAATAGGGGATATGGAACCTGGCAAGACTGAATCATTTTACTGTGAAATATA1800.10158414835800512No Hit
TCAAGAGGGATTACTGGGCAAATTGGCAAGATAATTATAGAATTGTCATTAA1800.10158414835800512No Hit
ACAACAGGGGGAGGTGTTCACCCGCTTCTGCACCGGCCTCACCCAGATTGAA1800.10158414835800512No Hit
CTTAAGGGGACTGTTCATCCAGTAAGCCAAGCCAGAAACCTAGGAGTCTTCT1800.10158414835800512No Hit
CAATCTGGGAGCGCGGGGAAATGAAATCTAAGATACATGAAGAAATTCTAAG1800.10158414835800512No Hit
GAAGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1800.10158414835800512No Hit
TTATGAAGGTGGCAGGCACTTCAAACCCTGGTGAGGAATTACCATTTTAAGC1790.10101979197823842No Hit
GTTCCTGGGAGATCACTATCATCTAATATTACCAATGTTTTCTTTTGTTTCT1790.10101979197823842No Hit
AAGCTAGGGGATCCATACGTTACGTTTACATGGTGCGAGGAAAGGGCACCCT1780.10045543559847171No Hit
TTTTACGGGACGGAACTTTCTCATCTACGTGGCCCTCCTGCGAGTCACTCCA1780.10045543559847171No Hit
ATACAAGGGGATCTTGCTTGGGGCTGTATCATAAGTTCAGCCTGTCATACTT1780.10045543559847171No Hit
CGATAGGGGGTGAAAAATTAGGTCTAAACATACAGTAATAAGAAAATAGTTA1780.10045543559847171No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)